Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-88 |
Sentence |
denotes |
Purification and regulatory properties of chicken heart prostaglandin E 9-ketoreductase. |
T2 |
89-259 |
Sentence |
denotes |
Prostaglandin E 9-ketoreductase was purified from chicken heart by ammonium sulfate fractionation, and DEAE-Sephadex, hydroxylapatite and phosphocellulose chromatography. |
T3 |
260-345 |
Sentence |
denotes |
Two peaks of activity were resolved during the phosphocellulose chromatographic step. |
T4 |
346-438 |
Sentence |
denotes |
Both peaks were stimulated by a substance that was not bound to the phosphocellulose column. |
T5 |
439-531 |
Sentence |
denotes |
This stimulatory substance was destroyed by treatment with phosphodiesterase and 0.1 M NaOH. |
T6 |
532-728 |
Sentence |
denotes |
It was heat-stable (100 degrees, 2 min), nondialyzable, and resistant to treatment with pronase, ribonuclease, and deoxyribonuclease; but it was dialyzable after heating or digestion with pronase. |
T7 |
729-864 |
Sentence |
denotes |
Sodium pyrophosphate also enhanced the activities of the prostaglandin E 9-ketoreductases as did angiotensin I; but not angiotensin II. |
T8 |
865-1132 |
Sentence |
denotes |
In the presence of 3':5'-cyclic AMP, AMP, or several other ribonucleotides, the enhancing effects of the natural stimulatory substance, sodium pyrophosphate or angiotensin I were blocked, but these ribonucleotides themselves had little effect on the enzymes activity. |
T9 |
1133-1223 |
Sentence |
denotes |
The substrate specificities of the two prostaglandin E 9-ketoreductases were also studied. |
T10 |
1224-1416 |
Sentence |
denotes |
Both the 9-keto group and the 15-keto group of 15-ketoprostaglandin F2 alpha could be converted to the corresponding hydroxyl group; the 15-keto group was reduced faster than the 9-keto group. |
T11 |
1417-1632 |
Sentence |
denotes |
Prostaglandin D2, a prostaglandin with a 9-hydroxyl and an 11-keto group, could not be converted to prostaglandin F2 alpha nor could cyclohexanone be converted to cyclohexanol by the prostaglandin E 9-ketoreductase. |
T1 |
0-88 |
Sentence |
denotes |
Purification and regulatory properties of chicken heart prostaglandin E 9-ketoreductase. |
T2 |
89-259 |
Sentence |
denotes |
Prostaglandin E 9-ketoreductase was purified from chicken heart by ammonium sulfate fractionation, and DEAE-Sephadex, hydroxylapatite and phosphocellulose chromatography. |
T3 |
260-345 |
Sentence |
denotes |
Two peaks of activity were resolved during the phosphocellulose chromatographic step. |
T4 |
346-438 |
Sentence |
denotes |
Both peaks were stimulated by a substance that was not bound to the phosphocellulose column. |
T5 |
439-531 |
Sentence |
denotes |
This stimulatory substance was destroyed by treatment with phosphodiesterase and 0.1 M NaOH. |
T6 |
532-728 |
Sentence |
denotes |
It was heat-stable (100 degrees, 2 min), nondialyzable, and resistant to treatment with pronase, ribonuclease, and deoxyribonuclease; but it was dialyzable after heating or digestion with pronase. |
T7 |
729-864 |
Sentence |
denotes |
Sodium pyrophosphate also enhanced the activities of the prostaglandin E 9-ketoreductases as did angiotensin I; but not angiotensin II. |
T8 |
865-1132 |
Sentence |
denotes |
In the presence of 3':5'-cyclic AMP, AMP, or several other ribonucleotides, the enhancing effects of the natural stimulatory substance, sodium pyrophosphate or angiotensin I were blocked, but these ribonucleotides themselves had little effect on the enzymes activity. |
T9 |
1133-1223 |
Sentence |
denotes |
The substrate specificities of the two prostaglandin E 9-ketoreductases were also studied. |
T10 |
1224-1416 |
Sentence |
denotes |
Both the 9-keto group and the 15-keto group of 15-ketoprostaglandin F2 alpha could be converted to the corresponding hydroxyl group; the 15-keto group was reduced faster than the 9-keto group. |
T11 |
1417-1632 |
Sentence |
denotes |
Prostaglandin D2, a prostaglandin with a 9-hydroxyl and an 11-keto group, could not be converted to prostaglandin F2 alpha nor could cyclohexanone be converted to cyclohexanol by the prostaglandin E 9-ketoreductase. |