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PubMed:16263187 / 136-1930 JSONTXT

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bionlp-st-bb3-2016-training

Id Subject Object Predicate Lexical cue
T2 0-1794 Paragraph denotes The ability of Lactobacillus (Lb.) gasseri K 7 to inhibit adhesion of Escherichia coli O8:K88 to intestinal mucosa was studied on cultured Caco-2 cells and ex vivo on pigs' small intestinal tissue. Lactobacilli were added simultaneously with E. coli, before E. coli and after E. coli for competition, exclusion and displacement assays. The concentration of lactobacilli on fully differentiated Caco-2 cells was 4.5+/-0.3 x 10(8) cfu/well, while the concentration of E. coli varied from 1.5 x 10(6) to 4.3 x 10(8) cfu/well. The number of E. coli adhered to Caco-2 monolayer (cfu/well) was lineary correlated (R(2)=0.97) to the concentration of added cells. In the assay simulating exclusion, E. coli adhesion was reduced by Lb. gasseri K 7 strain by 0.1 to 0.6 log cfu/well. The binding of E. coli was inhibited even more when incubated simultaneously with lactobacilli, particularly at the lowest concentration of E. coli (ratio E. coli/lactobacilli 1:248), where five-times reduction (or 0.7 log) was observed. When adhesion to tissue derived from pigs' jejunum was tested, concentration of E. coli was constant (6.9+/-0.14 x 10(7) cfu/ml), while the concentration of Lb. gasseri K 7 was 5.9 x 10(7) and 1.3 x 10(7) cfu/ml in two independent experiments, respectively. The adhesion of E. coli and Lb. gasseri K 7 cells to jejunal mucosa was similar (1.0+/-0.17 x 10(6) and 1.54+/-0.10 x 10(6) cfu/cm(2)) when the concentrations of single strains in suspensions were approximately the same. No significant competition, exclusion or displacement of E. coli by lactobacilli was observed on jejunal tissue. In conclusion, Lb. gasseri K 7 was found to be effective in reducing E. coli adhesion to Caco-2 enterocytes, but it was not able to do so in ex vivo conditions tested for pig jejunal tissue.
T9 15-46 Bacteria denotes Lactobacillus (Lb.) gasseri K 7
T10 70-93 Bacteria denotes Escherichia coli O8:K88
T11 97-107 Habitat denotes intestinal
T12 97-114 Habitat denotes intestinal mucosa
T13 130-151 Habitat denotes cultured Caco-2 cells
T14 167-171 Habitat denotes pigs
T15 167-196 Habitat denotes pigs' small intestinal tissue
T16 173-189 Habitat denotes small intestinal
T17 198-210 Bacteria denotes Lactobacilli
T18 242-249 Bacteria denotes E. coli
T19 258-265 Bacteria denotes E. coli
T20 276-283 Bacteria denotes E. coli
T21 357-369 Bacteria denotes lactobacilli
T22 373-406 Habitat denotes fully differentiated Caco-2 cells
T23 466-473 Bacteria denotes E. coli
T24 537-544 Bacteria denotes E. coli
T25 556-562 Habitat denotes Caco-2
T26 649-654 Habitat denotes cells
T27 691-698 Bacteria denotes E. coli
T28 723-738 Bacteria denotes Lb. gasseri K 7
T29 789-796 Bacteria denotes E. coli
T30 856-868 Bacteria denotes lactobacilli
T31 914-921 Bacteria denotes E. coli
T32 929-936 Bacteria denotes E. coli
T33 937-949 Bacteria denotes lactobacilli
T34 1029-1035 Habitat denotes tissue
T35 1049-1062 Habitat denotes pigs' jejunum
T36 1049-1053 Habitat denotes pigs
T37 1092-1099 Bacteria denotes E. coli
T38 1169-1184 Bacteria denotes Lb. gasseri K 7
T39 1286-1293 Bacteria denotes E. coli
T40 1298-1313 Bacteria denotes Lb. gasseri K 7
T41 1323-1337 Habitat denotes jejunal mucosa
T42 1323-1330 Habitat denotes jejunal
T43 1548-1555 Bacteria denotes E. coli
T44 1559-1571 Bacteria denotes lactobacilli
T45 1588-1595 Habitat denotes jejunal
T46 1588-1602 Habitat denotes jejunal tissue
T47 1619-1634 Bacteria denotes Lb. gasseri K 7
T48 1673-1680 Bacteria denotes E. coli
T49 1693-1711 Habitat denotes Caco-2 enterocytes
T50 1775-1778 Habitat denotes pig
T51 1775-1793 Habitat denotes pig jejunal tissue
T52 1779-1786 Habitat denotes jejunal