PubMed:16177809 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/16177809","sourcedb":"PubMed","sourceid":"16177809","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/16177809","text":"Atorvastatin downregulates tissue plasminogen activator-aggravated genes mediating coagulation and vascular permeability in single cerebral endothelial cells captured by laser microdissection.\nThe effects of statins on gene expression of cerebral endothelial cells (ECs) in vivo have not been investigated after stroke. We developed a rapid double immunofluorescent staining protocol with antibodies against von Willebrand factor (a marker for endothelium) and glial fibrillary acidic protein (a marker for astrocytes) for laser capture microdissection to isolate single ECs in brain tissue of the rat. Using this protocol in combination with real-time PCR, we found that stroke significantly increased mRNA levels of protease-activated receptor 1 (PAR-1) and tissue factor (TF) in ECs isolated from ischemic cerebral microvessels compared with nonischemic vessels. Treatment of embolic stroke with recombinant human tissue plasminogen activator (rht-PA) 4 h after stroke further elevated PAR-1 mRNA levels nearly 1000-fold in the core and 500-fold in the boundary above the nonstroke group 30 h after stroke, while TF mRNA levels were elevated approximately 10 fold above the nonstroke group. Furthermore, stroke significantly increased matrix metalloproteinase (MMP) 2 and 9 mRNA levels in the ischemic core and boundary regions 6 and 30 h after stroke. Treatment with rht-PA-upregulated MMP2 expression in the ischemic boundary and core. Atorvastatin completely blocked rht-PA upregulation of the above genes, when atorvastatin in combination with rht-PA was administered 4 h after stroke. Monotherapy of atorvastatin 4 h after stroke did not significantly reduce expression of genes examined in the present study. These data provide evidence that atorvastatin reduces exogenous tPA-aggravated cerebral endothelial genes that mediate thrombosis and blood-brain barrier permeability, which could contribute to the beneficial effects of statins on thrombolytic treatment of acute stroke.","tracks":[{"project":"UseCases_ArguminSci_Discourse","denotations":[{"id":"T1","span":{"begin":0,"end":192},"obj":"DRI_Background"},{"id":"T2","span":{"begin":193,"end":319},"obj":"DRI_Background"},{"id":"T3","span":{"begin":320,"end":602},"obj":"DRI_Challenge"},{"id":"T4","span":{"begin":603,"end":865},"obj":"DRI_Outcome"},{"id":"T5","span":{"begin":866,"end":1193},"obj":"DRI_Background"},{"id":"T6","span":{"begin":1194,"end":1355},"obj":"DRI_Background"},{"id":"T7","span":{"begin":1356,"end":1370},"obj":"DRI_Background"},{"id":"T8","span":{"begin":1371,"end":1394},"obj":"Token_Label.OUTSIDE"},{"id":"T9","span":{"begin":1395,"end":1440},"obj":"DRI_Background"},{"id":"T10","span":{"begin":1441,"end":1592},"obj":"DRI_Background"},{"id":"T11","span":{"begin":1593,"end":1717},"obj":"DRI_Background"},{"id":"T12","span":{"begin":1718,"end":1988},"obj":"DRI_Challenge"}],"attributes":[{"subj":"T1","pred":"source","obj":"UseCases_ArguminSci_Discourse"},{"subj":"T2","pred":"source","obj":"UseCases_ArguminSci_Discourse"},{"subj":"T3","pred":"source","obj":"UseCases_ArguminSci_Discourse"},{"subj":"T4","pred":"source","obj":"UseCases_ArguminSci_Discourse"},{"subj":"T5","pred":"source","obj":"UseCases_ArguminSci_Discourse"},{"subj":"T6","pred":"source","obj":"UseCases_ArguminSci_Discourse"},{"subj":"T7","pred":"source","obj":"UseCases_ArguminSci_Discourse"},{"subj":"T8","pred":"source","obj":"UseCases_ArguminSci_Discourse"},{"subj":"T9","pred":"source","obj":"UseCases_ArguminSci_Discourse"},{"subj":"T10","pred":"source","obj":"UseCases_ArguminSci_Discourse"},{"subj":"T11","pred":"source","obj":"UseCases_ArguminSci_Discourse"},{"subj":"T12","pred":"source","obj":"UseCases_ArguminSci_Discourse"}]},{"project":"c_corpus","denotations":[{"id":"T4","span":{"begin":0,"end":12},"obj":"83367"},{"id":"T1","span":{"begin":0,"end":12},"obj":"D000069059"},{"id":"T2","span":{"begin":0,"end":12},"obj":"CHEBI:39548"},{"id":"T3","span":{"begin":0,"end":12},"obj":"D000069059"},{"id":"T5","span":{"begin":27,"end":33},"obj":"UBERON:0000479"},{"id":"T6","span":{"begin":27,"end":55},"obj":"D010959"},{"id":"T7","span":{"begin":27,"end":55},"obj":"D010959"},{"id":"T26","span":{"begin":34,"end":55},"obj":"P17811"},{"id":"T28","span":{"begin":83,"end":94},"obj":"GO:0050817"},{"id":"T29","span":{"begin":208,"end":215},"obj":"D019161"},{"id":"T30","span":{"begin":208,"end":215},"obj":"CHEBI:87631"},{"id":"T31","span":{"begin":208,"end":215},"obj":"D019161"},{"id":"T32","span":{"begin":219,"end":223},"obj":"SO:0000704"},{"id":"T33","span":{"begin":219,"end":234},"obj":"GO:0010467"},{"id":"T34","span":{"begin":266,"end":269},"obj":"CVCL_Z893"},{"id":"T35","span":{"begin":341,"end":347},"obj":"SO:0000985"},{"id":"T36","span":{"begin":389,"end":399},"obj":"D000906"},{"id":"T37","span":{"begin":408,"end":422},"obj":"D014842"},{"id":"T38","span":{"begin":408,"end":422},"obj":"D014842"},{"id":"T45","span":{"begin":408,"end":429},"obj":"11274"},{"id":"T41","span":{"begin":408,"end":429},"obj":"Q8CIZ8"},{"id":"T42","span":{"begin":408,"end":429},"obj":"P04275"},{"id":"T43","span":{"begin":408,"end":429},"obj":"Q62935"},{"id":"T44","span":{"begin":408,"end":429},"obj":"Q28833"},{"id":"T46","span":{"begin":408,"end":429},"obj":"Q28295"},{"id":"T47","span":{"begin":408,"end":429},"obj":"P80012"},{"id":"T48","span":{"begin":408,"end":429},"obj":"PR:000017364"},{"id":"T39","span":{"begin":408,"end":429},"obj":"D014841"},{"id":"T40","span":{"begin":408,"end":429},"obj":"D014841"},{"id":"T51","span":{"begin":423,"end":432},"obj":"P18266"},{"id":"T52","span":{"begin":423,"end":432},"obj":"P18265"},{"id":"T49","span":{"begin":423,"end":432},"obj":"C413332"},{"id":"T50","span":{"begin":423,"end":432},"obj":"C413332"},{"id":"T53","span":{"begin":423,"end":432},"obj":"CHEBI:79381"},{"id":"T54","span":{"begin":444,"end":455},"obj":"UBERON:0001986"},{"id":"T55","span":{"begin":461,"end":492},"obj":"P47819"},{"id":"T57","span":{"begin":461,"end":492},"obj":"P14136"},{"id":"T58","span":{"begin":461,"end":492},"obj":"PR:000007939"},{"id":"T59","span":{"begin":461,"end":492},"obj":"Q28115"},{"id":"T60","span":{"begin":461,"end":492},"obj":"Q58EE9"},{"id":"T61","span":{"begin":461,"end":492},"obj":"Q5RA72"},{"id":"T63","span":{"begin":461,"end":492},"obj":"P03995"},{"id":"T64","span":{"begin":461,"end":492},"obj":"P48677"},{"id":"T56","span":{"begin":461,"end":492},"obj":"GO:0045101"},{"id":"T65","span":{"begin":461,"end":492},"obj":"GO:0045098"},{"id":"T62","span":{"begin":461,"end":492},"obj":"D005904"},{"id":"T70","span":{"begin":485,"end":492},"obj":"SO:0000104"},{"id":"T71","span":{"begin":485,"end":495},"obj":"Q9J7Z2"},{"id":"T72","span":{"begin":485,"end":495},"obj":"P03064"},{"id":"T73","span":{"begin":485,"end":495},"obj":"Q66929"},{"id":"T74","span":{"begin":485,"end":495},"obj":"Q9IMM4"},{"id":"T75","span":{"begin":485,"end":495},"obj":"Q9DIC5"},{"id":"T76","span":{"begin":485,"end":495},"obj":"Q86XJ0"},{"id":"T77","span":{"begin":485,"end":495},"obj":"Q992J0"},{"id":"T78","span":{"begin":485,"end":495},"obj":"P18164"},{"id":"T79","span":{"begin":485,"end":495},"obj":"Q96631"},{"id":"T80","span":{"begin":485,"end":495},"obj":"Q993M1"},{"id":"T83","span":{"begin":485,"end":495},"obj":"Q9QAZ8"},{"id":"T81","span":{"begin":485,"end":495},"obj":"D013205"},{"id":"T82","span":{"begin":485,"end":495},"obj":"D013205"},{"id":"T84","span":{"begin":571,"end":574},"obj":"CVCL_Z893"},{"id":"T85","span":{"begin":578,"end":583},"obj":"UBERON:6110636"},{"id":"T86","span":{"begin":578,"end":583},"obj":"UBERON:0000955"},{"id":"T87","span":{"begin":584,"end":590},"obj":"UBERON:0000479"},{"id":"T88","span":{"begin":598,"end":601},"obj":"10116"},{"id":"T92","span":{"begin":598,"end":601},"obj":"D051381"},{"id":"T89","span":{"begin":598,"end":601},"obj":"PR:P63003-1"},{"id":"T90","span":{"begin":598,"end":601},"obj":"PR:Q2G0B1"},{"id":"T91","span":{"begin":598,"end":601},"obj":"PR:Q8VHJ4"},{"id":"T93","span":{"begin":653,"end":656},"obj":"Q41249"},{"id":"T94","span":{"begin":653,"end":656},"obj":"O66148"},{"id":"T95","span":{"begin":653,"end":656},"obj":"Q59987"},{"id":"T96","span":{"begin":653,"end":656},"obj":"Q01289"},{"id":"T97","span":{"begin":653,"end":656},"obj":"O80333"},{"id":"T98","span":{"begin":653,"end":656},"obj":"P15904"},{"id":"T99","span":{"begin":653,"end":656},"obj":"Q9SDT1"},{"id":"T100","span":{"begin":653,"end":656},"obj":"Q39617"},{"id":"T103","span":{"begin":703,"end":707},"obj":"SO:0000234"},{"id":"T101","span":{"begin":703,"end":707},"obj":"D012333"},{"id":"T102","span":{"begin":703,"end":707},"obj":"CHEBI:33699"},{"id":"T104","span":{"begin":718,"end":726},"obj":"P68985"},{"id":"T105","span":{"begin":718,"end":726},"obj":"P30202"},{"id":"T106","span":{"begin":718,"end":726},"obj":"P42672"},{"id":"T107","span":{"begin":718,"end":726},"obj":"P11826"},{"id":"T108","span":{"begin":718,"end":726},"obj":"Q5TJ03"},{"id":"T109","span":{"begin":718,"end":726},"obj":"O71070"},{"id":"T110","span":{"begin":718,"end":726},"obj":"P63122"},{"id":"T111","span":{"begin":718,"end":726},"obj":"P63131"},{"id":"T112","span":{"begin":718,"end":726},"obj":"Q9IIH4"},{"id":"T113","span":{"begin":718,"end":726},"obj":"P10270"},{"id":"T114","span":{"begin":718,"end":726},"obj":"Q8JN66"},{"id":"T115","span":{"begin":718,"end":726},"obj":"O40958"},{"id":"T116","span":{"begin":718,"end":726},"obj":"P10265"},{"id":"T117","span":{"begin":718,"end":726},"obj":"Q9QM72"},{"id":"T118","span":{"begin":718,"end":726},"obj":"P63119"},{"id":"T119","span":{"begin":718,"end":726},"obj":"Q9WF19"},{"id":"T120","span":{"begin":718,"end":726},"obj":"P63120"},{"id":"T121","span":{"begin":718,"end":726},"obj":"P63125"},{"id":"T122","span":{"begin":718,"end":726},"obj":"P63124"},{"id":"T123","span":{"begin":718,"end":726},"obj":"P68986"},{"id":"T124","span":{"begin":718,"end":726},"obj":"Q96629"},{"id":"T125","span":{"begin":718,"end":726},"obj":"P63121"},{"id":"T126","span":{"begin":718,"end":726},"obj":"P09569"},{"id":"T127","span":{"begin":718,"end":726},"obj":"P03253"},{"id":"T128","span":{"begin":718,"end":726},"obj":"P63129"},{"id":"T129","span":{"begin":718,"end":726},"obj":"P10381"},{"id":"T130","span":{"begin":718,"end":726},"obj":"P07885"},{"id":"T131","span":{"begin":718,"end":726},"obj":"Q83906"},{"id":"T132","span":{"begin":718,"end":726},"obj":"P19119"},{"id":"T133","span":{"begin":718,"end":726},"obj":"P63127"},{"id":"T134","span":{"begin":718,"end":726},"obj":"Q84177"},{"id":"T135","span":{"begin":718,"end":726},"obj":"P19151"},{"id":"T136","span":{"begin":718,"end":726},"obj":"Q9Y6I0"},{"id":"T137","span":{"begin":718,"end":726},"obj":"P63123"},{"id":"T138","span":{"begin":718,"end":726},"obj":"P68984"},{"id":"T139","span":{"begin":718,"end":726},"obj":"P87563"},{"id":"T140","span":{"begin":718,"end":726},"obj":"P03252"},{"id":"T141","span":{"begin":718,"end":726},"obj":"O71150"},{"id":"T142","span":{"begin":718,"end":726},"obj":"P11825"},{"id":"T143","span":{"begin":718,"end":747},"obj":"D044463"},{"id":"T144","span":{"begin":749,"end":752},"obj":"CVCL_E801"},{"id":"T145","span":{"begin":749,"end":752},"obj":"44741"},{"id":"T146","span":{"begin":749,"end":754},"obj":"P25116"},{"id":"T147","span":{"begin":749,"end":754},"obj":"P56488"},{"id":"T148","span":{"begin":749,"end":754},"obj":"PR:Q9HAB3"},{"id":"T149","span":{"begin":749,"end":754},"obj":"PR:P19880"},{"id":"T150","span":{"begin":749,"end":754},"obj":"PR:P25116"},{"id":"T151","span":{"begin":749,"end":754},"obj":"PR:P30558"},{"id":"T152","span":{"begin":749,"end":754},"obj":"PR:P26824"},{"id":"T153","span":{"begin":749,"end":754},"obj":"PR:Q10428"},{"id":"T154","span":{"begin":749,"end":754},"obj":"PR:Q1MTN7"},{"id":"T155","span":{"begin":749,"end":754},"obj":"PR:Q9TW45"},{"id":"T156","span":{"begin":749,"end":754},"obj":"P30558"},{"id":"T157","span":{"begin":749,"end":754},"obj":"P47749"},{"id":"T158","span":{"begin":749,"end":754},"obj":"PR:Q9SJH0"},{"id":"T159","span":{"begin":749,"end":754},"obj":"P26824"},{"id":"T160","span":{"begin":749,"end":754},"obj":"PR:000001661"},{"id":"T161","span":{"begin":749,"end":754},"obj":"PR:000008187"},{"id":"T162","span":{"begin":749,"end":754},"obj":"Q00991"},{"id":"T163","span":{"begin":749,"end":754},"obj":"A7YY44"},{"id":"T164","span":{"begin":760,"end":766},"obj":"UBERON:0000479"},{"id":"T165","span":{"begin":760,"end":773},"obj":"P30931"},{"id":"T166","span":{"begin":760,"end":773},"obj":"P13726"},{"id":"T167","span":{"begin":760,"end":773},"obj":"Q9JLU8"},{"id":"T168","span":{"begin":760,"end":773},"obj":"P24055"},{"id":"T169","span":{"begin":760,"end":773},"obj":"P42533"},{"id":"T171","span":{"begin":760,"end":773},"obj":"P20352"},{"id":"T173","span":{"begin":760,"end":773},"obj":"PR:000001940"},{"id":"T170","span":{"begin":760,"end":773},"obj":"D013925"},{"id":"T172","span":{"begin":760,"end":773},"obj":"D013925"},{"id":"T174","span":{"begin":782,"end":785},"obj":"CVCL_Z893"},{"id":"T175","span":{"begin":911,"end":916},"obj":"D006801"},{"id":"T176","span":{"begin":917,"end":923},"obj":"UBERON:0000479"},{"id":"T177","span":{"begin":917,"end":945},"obj":"D010959"},{"id":"T178","span":{"begin":917,"end":945},"obj":"D010959"},{"id":"T197","span":{"begin":924,"end":945},"obj":"P17811"},{"id":"T199","span":{"begin":951,"end":956},"obj":"CVCL_D695"},{"id":"T200","span":{"begin":989,"end":992},"obj":"CVCL_E801"},{"id":"T201","span":{"begin":989,"end":992},"obj":"44741"},{"id":"T202","span":{"begin":989,"end":994},"obj":"P25116"},{"id":"T203","span":{"begin":989,"end":994},"obj":"P56488"},{"id":"T204","span":{"begin":989,"end":994},"obj":"PR:Q9HAB3"},{"id":"T205","span":{"begin":989,"end":994},"obj":"PR:P19880"},{"id":"T206","span":{"begin":989,"end":994},"obj":"PR:P25116"},{"id":"T207","span":{"begin":989,"end":994},"obj":"PR:P30558"},{"id":"T208","span":{"begin":989,"end":994},"obj":"PR:P26824"},{"id":"T209","span":{"begin":989,"end":994},"obj":"PR:Q10428"},{"id":"T210","span":{"begin":989,"end":994},"obj":"PR:Q1MTN7"},{"id":"T211","span":{"begin":989,"end":994},"obj":"PR:Q9TW45"},{"id":"T212","span":{"begin":989,"end":994},"obj":"P30558"},{"id":"T213","span":{"begin":989,"end":994},"obj":"P47749"},{"id":"T214","span":{"begin":989,"end":994},"obj":"PR:Q9SJH0"},{"id":"T215","span":{"begin":989,"end":994},"obj":"P26824"},{"id":"T216","span":{"begin":989,"end":994},"obj":"PR:000001661"},{"id":"T217","span":{"begin":989,"end":994},"obj":"PR:000008187"},{"id":"T218","span":{"begin":989,"end":994},"obj":"Q00991"},{"id":"T219","span":{"begin":989,"end":994},"obj":"A7YY44"},{"id":"T222","span":{"begin":995,"end":999},"obj":"SO:0000234"},{"id":"T220","span":{"begin":995,"end":999},"obj":"D012333"},{"id":"T221","span":{"begin":995,"end":999},"obj":"CHEBI:33699"},{"id":"T223","span":{"begin":1019,"end":1023},"obj":"PR:000022679"},{"id":"T224","span":{"begin":1019,"end":1023},"obj":"PR:Q2FZJ6"},{"id":"T225","span":{"begin":1019,"end":1023},"obj":"PR:A5I112"},{"id":"T226","span":{"begin":1019,"end":1023},"obj":"PR:P24186"},{"id":"T227","span":{"begin":1031,"end":1035},"obj":"GO:0019013"},{"id":"T228","span":{"begin":1044,"end":1048},"obj":"PR:000022679"},{"id":"T229","span":{"begin":1044,"end":1048},"obj":"PR:Q2FZJ6"},{"id":"T230","span":{"begin":1044,"end":1048},"obj":"PR:A5I112"},{"id":"T231","span":{"begin":1044,"end":1048},"obj":"PR:P24186"},{"id":"T232","span":{"begin":1056,"end":1064},"obj":"SO:0000699"},{"id":"T235","span":{"begin":1119,"end":1123},"obj":"SO:0000234"},{"id":"T233","span":{"begin":1119,"end":1123},"obj":"D012333"},{"id":"T234","span":{"begin":1119,"end":1123},"obj":"CHEBI:33699"},{"id":"T236","span":{"begin":1162,"end":1166},"obj":"PR:000022679"},{"id":"T237","span":{"begin":1162,"end":1166},"obj":"PR:Q2FZJ6"},{"id":"T238","span":{"begin":1162,"end":1166},"obj":"PR:A5I112"},{"id":"T239","span":{"begin":1162,"end":1166},"obj":"PR:P24186"},{"id":"T240","span":{"begin":1238,"end":1244},"obj":"UBERON:0027368"},{"id":"T241","span":{"begin":1245,"end":1270},"obj":"D020778"},{"id":"T246","span":{"begin":1264,"end":1270},"obj":"PR:P08253"},{"id":"T247","span":{"begin":1264,"end":1270},"obj":"Q9GLE5"},{"id":"T248","span":{"begin":1264,"end":1270},"obj":"P08253"},{"id":"T249","span":{"begin":1264,"end":1270},"obj":"Q90611"},{"id":"T251","span":{"begin":1264,"end":1270},"obj":"PR:Q8MPP3"},{"id":"T252","span":{"begin":1264,"end":1270},"obj":"P33436"},{"id":"T253","span":{"begin":1264,"end":1270},"obj":"PR:Q90611"},{"id":"T254","span":{"begin":1264,"end":1270},"obj":"PR:P33436"},{"id":"T255","span":{"begin":1264,"end":1270},"obj":"PR:000010479"},{"id":"T256","span":{"begin":1264,"end":1270},"obj":"PR:P33434"},{"id":"T257","span":{"begin":1264,"end":1270},"obj":"P33434"},{"id":"T258","span":{"begin":1264,"end":1270},"obj":"P50757"},{"id":"T250","span":{"begin":1264,"end":1270},"obj":"GO:0004228"},{"id":"T261","span":{"begin":1277,"end":1281},"obj":"SO:0000234"},{"id":"T259","span":{"begin":1277,"end":1281},"obj":"D012333"},{"id":"T260","span":{"begin":1277,"end":1281},"obj":"CHEBI:33699"},{"id":"T262","span":{"begin":1305,"end":1309},"obj":"GO:0019013"},{"id":"T263","span":{"begin":1314,"end":1322},"obj":"SO:0000699"},{"id":"T264","span":{"begin":1390,"end":1393},"obj":"CHEBI:340824"},{"id":"T267","span":{"begin":1390,"end":1393},"obj":"CHEBI:59761"},{"id":"T268","span":{"begin":1390,"end":1394},"obj":"PR:P08253"},{"id":"T269","span":{"begin":1390,"end":1394},"obj":"Q9GLE5"},{"id":"T270","span":{"begin":1390,"end":1394},"obj":"P08253"},{"id":"T271","span":{"begin":1390,"end":1394},"obj":"Q90611"},{"id":"T273","span":{"begin":1390,"end":1394},"obj":"PR:Q8MPP3"},{"id":"T274","span":{"begin":1390,"end":1394},"obj":"P33436"},{"id":"T275","span":{"begin":1390,"end":1394},"obj":"PR:Q90611"},{"id":"T276","span":{"begin":1390,"end":1394},"obj":"PR:P33436"},{"id":"T277","span":{"begin":1390,"end":1394},"obj":"PR:000010479"},{"id":"T278","span":{"begin":1390,"end":1394},"obj":"PR:P33434"},{"id":"T279","span":{"begin":1390,"end":1394},"obj":"P33434"},{"id":"T280","span":{"begin":1390,"end":1394},"obj":"P50757"},{"id":"T272","span":{"begin":1390,"end":1394},"obj":"GO:0004228"},{"id":"T281","span":{"begin":1422,"end":1430},"obj":"SO:0000699"},{"id":"T282","span":{"begin":1435,"end":1439},"obj":"GO:0019013"},{"id":"T286","span":{"begin":1441,"end":1453},"obj":"83367"},{"id":"T283","span":{"begin":1441,"end":1453},"obj":"D000069059"},{"id":"T284","span":{"begin":1441,"end":1453},"obj":"CHEBI:39548"},{"id":"T285","span":{"begin":1441,"end":1453},"obj":"D000069059"},{"id":"T290","span":{"begin":1518,"end":1530},"obj":"83367"},{"id":"T287","span":{"begin":1518,"end":1530},"obj":"D000069059"},{"id":"T288","span":{"begin":1518,"end":1530},"obj":"CHEBI:39548"},{"id":"T289","span":{"begin":1518,"end":1530},"obj":"D000069059"},{"id":"T294","span":{"begin":1608,"end":1620},"obj":"83367"},{"id":"T291","span":{"begin":1608,"end":1620},"obj":"D000069059"},{"id":"T292","span":{"begin":1608,"end":1620},"obj":"CHEBI:39548"},{"id":"T293","span":{"begin":1608,"end":1620},"obj":"D000069059"},{"id":"T298","span":{"begin":1751,"end":1763},"obj":"83367"},{"id":"T295","span":{"begin":1751,"end":1763},"obj":"D000069059"},{"id":"T296","span":{"begin":1751,"end":1763},"obj":"CHEBI:39548"},{"id":"T297","span":{"begin":1751,"end":1763},"obj":"D000069059"},{"id":"T302","span":{"begin":1782,"end":1785},"obj":"Q28198"},{"id":"T304","span":{"begin":1782,"end":1785},"obj":"Q5R8J0"},{"id":"T305","span":{"begin":1782,"end":1785},"obj":"P19637"},{"id":"T306","span":{"begin":1782,"end":1785},"obj":"P00750"},{"id":"T307","span":{"begin":1782,"end":1785},"obj":"Q8SQ23"},{"id":"T309","span":{"begin":1782,"end":1785},"obj":"PR:000012825"},{"id":"T310","span":{"begin":1782,"end":1785},"obj":"P11214"},{"id":"T299","span":{"begin":1782,"end":1785},"obj":"CHEBI:53394"},{"id":"T300","span":{"begin":1782,"end":1785},"obj":"CHEBI:15702"},{"id":"T301","span":{"begin":1782,"end":1785},"obj":"CHEBI:61432"},{"id":"T303","span":{"begin":1782,"end":1785},"obj":"CHEBI:37537"},{"id":"T308","span":{"begin":1782,"end":1785},"obj":"GO:0031299"},{"id":"T311","span":{"begin":1837,"end":1847},"obj":"D013927"},{"id":"T312","span":{"begin":1837,"end":1847},"obj":"D013927"},{"id":"T313","span":{"begin":1852,"end":1871},"obj":"UBERON:0000120"},{"id":"T316","span":{"begin":1938,"end":1945},"obj":"D019161"},{"id":"T317","span":{"begin":1938,"end":1945},"obj":"CHEBI:87631"},{"id":"T318","span":{"begin":1938,"end":1945},"obj":"D019161"},{"id":"T319","span":{"begin":1975,"end":1987},"obj":"D020521"},{"id":"T320","span":{"begin":1975,"end":1987},"obj":"D020521"}],"attributes":[{"subj":"T4","pred":"source","obj":"c_corpus"},{"subj":"T1","pred":"source","obj":"c_corpus"},{"subj":"T2","pred":"source","obj":"c_corpus"},{"subj":"T3","pred":"source","obj":"c_corpus"},{"subj":"T5","pred":"source","obj":"c_corpus"},{"subj":"T6","pred":"source","obj":"c_corpus"},{"subj":"T7","pred":"source","obj":"c_corpus"},{"subj":"T26","pred":"source","obj":"c_corpus"},{"subj":"T28","pred":"source","obj":"c_corpus"},{"subj":"T29","pred":"source","obj":"c_corpus"},{"subj":"T30","pred":"source","obj":"c_corpus"},{"subj":"T31","pred":"source","obj":"c_corpus"},{"subj":"T32","pred":"source","obj":"c_corpus"},{"subj":"T33","pred":"source","obj":"c_corpus"},{"subj":"T34","pred":"source","obj":"c_corpus"},{"subj":"T35","pred":"source","obj":"c_corpus"},{"subj":"T36","pred":"source","obj":"c_corpus"},{"subj":"T37","pred":"source","obj":"c_corpus"},{"subj":"T38","pred":"source","obj":"c_corpus"},{"subj":"T45","pred":"source","obj":"c_corpus"},{"subj":"T41","pred":"source","obj":"c_corpus"},{"subj":"T42","pred":"source","obj":"c_corpus"},{"subj":"T43","pred":"source","obj":"c_corpus"},{"subj":"T44","pred":"source","obj":"c_corpus"},{"subj":"T46","pred":"source","obj":"c_corpus"},{"subj":"T47","pred":"source","obj":"c_corpus"},{"subj":"T48","pred":"source","obj":"c_corpus"},{"subj":"T39","pred":"source","obj":"c_corpus"},{"subj":"T40","pred":"source","obj":"c_corpus"},{"subj":"T51","pred":"source","obj":"c_corpus"},{"subj":"T52","pred":"source","obj":"c_corpus"},{"subj":"T49","pred":"source","obj":"c_corpus"},{"subj":"T50","pred":"source","obj":"c_corpus"},{"subj":"T53","pred":"source","obj":"c_corpus"},{"subj":"T54","pred":"source","obj":"c_corpus"},{"subj":"T55","pred":"source","obj":"c_corpus"},{"subj":"T57","pred":"source","obj":"c_corpus"},{"subj":"T58","pred":"source","obj":"c_corpus"},{"subj":"T59","pred":"source","obj":"c_corpus"},{"subj":"T60","pred":"source","obj":"c_corpus"},{"subj":"T61","pred":"source","obj":"c_corpus"},{"subj":"T63","pred":"source","obj":"c_corpus"},{"subj":"T64","pred":"source","obj":"c_corpus"},{"subj":"T56","pred":"source","obj":"c_corpus"},{"subj":"T65","pred":"source","obj":"c_corpus"},{"subj":"T62","pred":"source","obj":"c_corpus"},{"subj":"T70","pred":"source","obj":"c_corpus"},{"subj":"T71","pred":"source","obj":"c_corpus"},{"subj":"T72","pred":"source","obj":"c_corpus"},{"subj":"T73","pred":"source","obj":"c_corpus"},{"subj":"T74","pred":"source","obj":"c_corpus"},{"subj":"T75","pred":"source","obj":"c_corpus"},{"subj":"T76","pred":"source","obj":"c_corpus"},{"subj":"T77","pred":"source","obj":"c_corpus"},{"subj":"T78","pred":"source","obj":"c_corpus"},{"subj":"T79","pred":"source","obj":"c_corpus"},{"subj":"T80","pred":"source","obj":"c_corpus"},{"subj":"T83","pred":"source","obj":"c_corpus"},{"subj":"T81","pred":"source","obj":"c_corpus"},{"subj":"T82","pred":"source","obj":"c_corpus"},{"subj":"T84","pred":"source","obj":"c_corpus"},{"subj":"T85","pred":"source","obj":"c_corpus"},{"subj":"T86","pred":"source","obj":"c_corpus"},{"subj":"T87","pred":"source","obj":"c_corpus"},{"subj":"T88","pred":"source","obj":"c_corpus"},{"subj":"T92","pred":"source","obj":"c_corpus"},{"subj":"T89","pred":"source","obj":"c_corpus"},{"subj":"T90","pred":"source","obj":"c_corpus"},{"subj":"T91","pred":"source","obj":"c_corpus"},{"subj":"T93","pred":"source","obj":"c_corpus"},{"subj":"T94","pred":"source","obj":"c_corpus"},{"subj":"T95","pred":"source","obj":"c_corpus"},{"subj":"T96","pred":"source","obj":"c_corpus"},{"subj":"T97","pred":"source","obj":"c_corpus"},{"subj":"T98","pred":"source","obj":"c_corpus"},{"subj":"T99","pred":"source","obj":"c_corpus"},{"subj":"T100","pred":"source","obj":"c_corpus"},{"subj":"T103","pred":"source","obj":"c_corpus"},{"subj":"T101","pred":"source","obj":"c_corpus"},{"subj":"T102","pred":"source","obj":"c_corpus"},{"subj":"T104","pred":"source","obj":"c_corpus"},{"subj":"T105","pred":"source","obj":"c_corpus"},{"subj":"T106","pred":"source","obj":"c_corpus"},{"subj":"T107","pred":"source","obj":"c_corpus"},{"subj":"T108","pred":"source","obj":"c_corpus"},{"subj":"T109","pred":"source","obj":"c_corpus"},{"subj":"T110","pred":"source","obj":"c_corpus"},{"subj":"T111","pred":"source","obj":"c_corpus"},{"subj":"T112","pred":"source","obj":"c_corpus"},{"subj":"T113","pred":"source","obj":"c_corpus"},{"subj":"T114","pred":"source","obj":"c_corpus"},{"subj":"T115","pred":"source","obj":"c_corpus"},{"subj":"T116","pred":"source","obj":"c_corpus"},{"subj":"T117","pred":"source","obj":"c_corpus"},{"subj":"T118","pred":"source","obj":"c_corpus"},{"subj":"T119","pred":"source","obj":"c_corpus"},{"subj":"T120","pred":"source","obj":"c_corpus"},{"subj":"T121","pred":"source","obj":"c_corpus"},{"subj":"T122","pred":"source","obj":"c_corpus"},{"subj":"T123","pred":"source","obj":"c_corpus"},{"subj":"T124","pred":"source","obj":"c_corpus"},{"subj":"T125","pred":"source","obj":"c_corpus"},{"subj":"T126","pred":"source","obj":"c_corpus"},{"subj":"T127","pred":"source","obj":"c_corpus"},{"subj":"T128","pred":"source","obj":"c_corpus"},{"subj":"T129","pred":"source","obj":"c_corpus"},{"subj":"T130","pred":"source","obj":"c_corpus"},{"subj":"T131","pred":"source","obj":"c_corpus"},{"subj":"T132","pred":"source","obj":"c_corpus"},{"subj":"T133","pred":"source","obj":"c_corpus"},{"subj":"T134","pred":"source","obj":"c_corpus"},{"subj":"T135","pred":"source","obj":"c_corpus"},{"subj":"T136","pred":"source","obj":"c_corpus"},{"subj":"T137","pred":"source","obj":"c_corpus"},{"subj":"T138","pred":"source","obj":"c_corpus"},{"subj":"T139","pred":"source","obj":"c_corpus"},{"subj":"T140","pred":"source","obj":"c_corpus"},{"subj":"T141","pred":"source","obj":"c_corpus"},{"subj":"T142","pred":"source","obj":"c_corpus"},{"subj":"T143","pred":"source","obj":"c_corpus"},{"subj":"T144","pred":"source","obj":"c_corpus"},{"subj":"T145","pred":"source","obj":"c_corpus"},{"subj":"T146","pred":"source","obj":"c_corpus"},{"subj":"T147","pred":"source","obj":"c_corpus"},{"subj":"T148","pred":"source","obj":"c_corpus"},{"subj":"T149","pred":"source","obj":"c_corpus"},{"subj":"T150","pred":"source","obj":"c_corpus"},{"subj":"T151","pred":"source","obj":"c_corpus"},{"subj":"T152","pred":"source","obj":"c_corpus"},{"subj":"T153","pred":"source","obj":"c_corpus"},{"subj":"T154","pred":"source","obj":"c_corpus"},{"subj":"T155","pred":"source","obj":"c_corpus"},{"subj":"T156","pred":"source","obj":"c_corpus"},{"subj":"T157","pred":"source","obj":"c_corpus"},{"subj":"T158","pred":"source","obj":"c_corpus"},{"subj":"T159","pred":"source","obj":"c_corpus"},{"subj":"T160","pred":"source","obj":"c_corpus"},{"subj":"T161","pred":"source","obj":"c_corpus"},{"subj":"T162","pred":"source","obj":"c_corpus"},{"subj":"T163","pred":"source","obj":"c_corpus"},{"subj":"T164","pred":"source","obj":"c_corpus"},{"subj":"T165","pred":"source","obj":"c_corpus"},{"subj":"T166","pred":"source","obj":"c_corpus"},{"subj":"T167","pred":"source","obj":"c_corpus"},{"subj":"T168","pred":"source","obj":"c_corpus"},{"subj":"T169","pred":"source","obj":"c_corpus"},{"subj":"T171","pred":"source","obj":"c_corpus"},{"subj":"T173","pred":"source","obj":"c_corpus"},{"subj":"T170","pred":"source","obj":"c_corpus"},{"subj":"T172","pred":"source","obj":"c_corpus"},{"subj":"T174","pred":"source","obj":"c_corpus"},{"subj":"T175","pred":"source","obj":"c_corpus"},{"subj":"T176","pred":"source","obj":"c_corpus"},{"subj":"T177","pred":"source","obj":"c_corpus"},{"subj":"T178","pred":"source","obj":"c_corpus"},{"subj":"T197","pred":"source","obj":"c_corpus"},{"subj":"T199","pred":"source","obj":"c_corpus"},{"subj":"T200","pred":"source","obj":"c_corpus"},{"subj":"T201","pred":"source","obj":"c_corpus"},{"subj":"T202","pred":"source","obj":"c_corpus"},{"subj":"T203","pred":"source","obj":"c_corpus"},{"subj":"T204","pred":"source","obj":"c_corpus"},{"subj":"T205","pred":"source","obj":"c_corpus"},{"subj":"T206","pred":"source","obj":"c_corpus"},{"subj":"T207","pred":"source","obj":"c_corpus"},{"subj":"T208","pred":"source","obj":"c_corpus"},{"subj":"T209","pred":"source","obj":"c_corpus"},{"subj":"T210","pred":"source","obj":"c_corpus"},{"subj":"T211","pred":"source","obj":"c_corpus"},{"subj":"T212","pred":"source","obj":"c_corpus"},{"subj":"T213","pred":"source","obj":"c_corpus"},{"subj":"T214","pred":"source","obj":"c_corpus"},{"subj":"T215","pred":"source","obj":"c_corpus"},{"subj":"T216","pred":"source","obj":"c_corpus"},{"subj":"T217","pred":"source","obj":"c_corpus"},{"subj":"T218","pred":"source","obj":"c_corpus"},{"subj":"T219","pred":"source","obj":"c_corpus"},{"subj":"T222","pred":"source","obj":"c_corpus"},{"subj":"T220","pred":"source","obj":"c_corpus"},{"subj":"T221","pred":"source","obj":"c_corpus"},{"subj":"T223","pred":"source","obj":"c_corpus"},{"subj":"T224","pred":"source","obj":"c_corpus"},{"subj":"T225","pred":"source","obj":"c_corpus"},{"subj":"T226","pred":"source","obj":"c_corpus"},{"subj":"T227","pred":"source","obj":"c_corpus"},{"subj":"T228","pred":"source","obj":"c_corpus"},{"subj":"T229","pred":"source","obj":"c_corpus"},{"subj":"T230","pred":"source","obj":"c_corpus"},{"subj":"T231","pred":"source","obj":"c_corpus"},{"subj":"T232","pred":"source","obj":"c_corpus"},{"subj":"T235","pred":"source","obj":"c_corpus"},{"subj":"T233","pred":"source","obj":"c_corpus"},{"subj":"T234","pred":"source","obj":"c_corpus"},{"subj":"T236","pred":"source","obj":"c_corpus"},{"subj":"T237","pred":"source","obj":"c_corpus"},{"subj":"T238","pred":"source","obj":"c_corpus"},{"subj":"T239","pred":"source","obj":"c_corpus"},{"subj":"T240","pred":"source","obj":"c_corpus"},{"subj":"T241","pred":"source","obj":"c_corpus"},{"subj":"T246","pred":"source","obj":"c_corpus"},{"subj":"T247","pred":"source","obj":"c_corpus"},{"subj":"T248","pred":"source","obj":"c_corpus"},{"subj":"T249","pred":"source","obj":"c_corpus"},{"subj":"T251","pred":"source","obj":"c_corpus"},{"subj":"T252","pred":"source","obj":"c_corpus"},{"subj":"T253","pred":"source","obj":"c_corpus"},{"subj":"T254","pred":"source","obj":"c_corpus"},{"subj":"T255","pred":"source","obj":"c_corpus"},{"subj":"T256","pred":"source","obj":"c_corpus"},{"subj":"T257","pred":"source","obj":"c_corpus"},{"subj":"T258","pred":"source","obj":"c_corpus"},{"subj":"T250","pred":"source","obj":"c_corpus"},{"subj":"T261","pred":"source","obj":"c_corpus"},{"subj":"T259","pred":"source","obj":"c_corpus"},{"subj":"T260","pred":"source","obj":"c_corpus"},{"subj":"T262","pred":"source","obj":"c_corpus"},{"subj":"T263","pred":"source","obj":"c_corpus"},{"subj":"T264","pred":"source","obj":"c_corpus"},{"subj":"T267","pred":"source","obj":"c_corpus"},{"subj":"T268","pred":"source","obj":"c_corpus"},{"subj":"T269","pred":"source","obj":"c_corpus"},{"subj":"T270","pred":"source","obj":"c_corpus"},{"subj":"T271","pred":"source","obj":"c_corpus"},{"subj":"T273","pred":"source","obj":"c_corpus"},{"subj":"T274","pred":"source","obj":"c_corpus"},{"subj":"T275","pred":"source","obj":"c_corpus"},{"subj":"T276","pred":"source","obj":"c_corpus"},{"subj":"T277","pred":"source","obj":"c_corpus"},{"subj":"T278","pred":"source","obj":"c_corpus"},{"subj":"T279","pred":"source","obj":"c_corpus"},{"subj":"T280","pred":"source","obj":"c_corpus"},{"subj":"T272","pred":"source","obj":"c_corpus"},{"subj":"T281","pred":"source","obj":"c_corpus"},{"subj":"T282","pred":"source","obj":"c_corpus"},{"subj":"T286","pred":"source","obj":"c_corpus"},{"subj":"T283","pred":"source","obj":"c_corpus"},{"subj":"T284","pred":"source","obj":"c_corpus"},{"subj":"T285","pred":"source","obj":"c_corpus"},{"subj":"T290","pred":"source","obj":"c_corpus"},{"subj":"T287","pred":"source","obj":"c_corpus"},{"subj":"T288","pred":"source","obj":"c_corpus"},{"subj":"T289","pred":"source","obj":"c_corpus"},{"subj":"T294","pred":"source","obj":"c_corpus"},{"subj":"T291","pred":"source","obj":"c_corpus"},{"subj":"T292","pred":"source","obj":"c_corpus"},{"subj":"T293","pred":"source","obj":"c_corpus"},{"subj":"T298","pred":"source","obj":"c_corpus"},{"subj":"T295","pred":"source","obj":"c_corpus"},{"subj":"T296","pred":"source","obj":"c_corpus"},{"subj":"T297","pred":"source","obj":"c_corpus"},{"subj":"T302","pred":"source","obj":"c_corpus"},{"subj":"T304","pred":"source","obj":"c_corpus"},{"subj":"T305","pred":"source","obj":"c_corpus"},{"subj":"T306","pred":"source","obj":"c_corpus"},{"subj":"T307","pred":"source","obj":"c_corpus"},{"subj":"T309","pred":"source","obj":"c_corpus"},{"subj":"T310","pred":"source","obj":"c_corpus"},{"subj":"T299","pred":"source","obj":"c_corpus"},{"subj":"T300","pred":"source","obj":"c_corpus"},{"subj":"T301","pred":"source","obj":"c_corpus"},{"subj":"T303","pred":"source","obj":"c_corpus"},{"subj":"T308","pred":"source","obj":"c_corpus"},{"subj":"T311","pred":"source","obj":"c_corpus"},{"subj":"T312","pred":"source","obj":"c_corpus"},{"subj":"T313","pred":"source","obj":"c_corpus"},{"subj":"T316","pred":"source","obj":"c_corpus"},{"subj":"T317","pred":"source","obj":"c_corpus"},{"subj":"T318","pred":"source","obj":"c_corpus"},{"subj":"T319","pred":"source","obj":"c_corpus"},{"subj":"T320","pred":"source","obj":"c_corpus"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"UseCases_ArguminSci_Discourse","color":"#93b1ec","default":true},{"id":"c_corpus","color":"#cbec93"}]}]}}