PubMed:15993867 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/15993867","sourcedb":"PubMed","sourceid":"15993867","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/15993867","text":"N-Acyl derivatives of glucosamine as acceptor substrates for galactosyltransferase from bone and cartilage cells.\nGlucosamine is commonly used as a nutraceutical by arthritis patients. However, its mode of action is still unknown, and there is controversy about its clinical efficacy. Synthetic N-acyl glucosamines (acyl group\u003e2 carbons) comprise a new class of drugs. We examined these derivatives for their effect in bone and cartilage cells, and for their ability to serve as acceptor substrates for galactosyltransferase. With the exception of N-benzoylglucosamine, compounds of the series were good substrates for galactosyltransferases from bone and cartilage cells, and for purified enzyme from bovine milk. When N-butyrylglucosamine (GlcNBu) was added to the cell medium of primary bovine chondrocytes and human osteoblasts, small amounts were found to enter the cells and a radiolabeled metabolite appeared in the medium. However, GlcNBu did not appear to be incorporated directly into oligosaccharides. GlcNBu at 1 and 5mM concentrations in the glucose-free cell medium of primary human osteoblasts from osteoarthritis patients did not significantly alter cell proliferation or cell differentiation.","tracks":[{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":1055,"end":1062},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG"},{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"}]},{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":88,"end":92},"obj":"http://purl.obolibrary.org/obo/MAT_0000299"},{"id":"T2","span":{"begin":97,"end":106},"obj":"http://purl.obolibrary.org/obo/MAT_0000189"},{"id":"T3","span":{"begin":419,"end":423},"obj":"http://purl.obolibrary.org/obo/MAT_0000299"},{"id":"T4","span":{"begin":428,"end":437},"obj":"http://purl.obolibrary.org/obo/MAT_0000189"},{"id":"T5","span":{"begin":647,"end":651},"obj":"http://purl.obolibrary.org/obo/MAT_0000299"},{"id":"T6","span":{"begin":656,"end":665},"obj":"http://purl.obolibrary.org/obo/MAT_0000189"}],"attributes":[{"subj":"T1","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T2","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T3","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T4","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T5","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T6","pred":"source","obj":"Glycosmos6-MAT"}]},{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":113},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":114,"end":184},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":185,"end":284},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":285,"end":368},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":369,"end":525},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":526,"end":714},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":715,"end":930},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":931,"end":1012},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1013,"end":1209},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":113},"obj":"Sentence"},{"id":"T2","span":{"begin":114,"end":184},"obj":"Sentence"},{"id":"T3","span":{"begin":185,"end":284},"obj":"Sentence"},{"id":"T4","span":{"begin":285,"end":368},"obj":"Sentence"},{"id":"T5","span":{"begin":369,"end":525},"obj":"Sentence"},{"id":"T6","span":{"begin":526,"end":714},"obj":"Sentence"},{"id":"T7","span":{"begin":715,"end":930},"obj":"Sentence"},{"id":"T8","span":{"begin":931,"end":1012},"obj":"Sentence"},{"id":"T9","span":{"begin":1013,"end":1209},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T9","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"}]},{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":1055,"end":1062},"obj":"https://glytoucan.org/Structures/Glycans/G15021LG"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"}]},{"project":"GlyCosmos15-HP","denotations":[{"id":"T1","span":{"begin":165,"end":174},"obj":"Phenotype"},{"id":"T2","span":{"begin":1114,"end":1128},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0001369"},{"id":"A2","pred":"hp_id","subj":"T2","obj":"HP:0002758"},{"subj":"T1","pred":"source","obj":"GlyCosmos15-HP"},{"subj":"T2","pred":"source","obj":"GlyCosmos15-HP"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}]},{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":165,"end":174},"obj":"Disease"},{"id":"T2","span":{"begin":1114,"end":1128},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0005578"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0005178"},{"subj":"T1","pred":"source","obj":"mondo_disease"},{"subj":"T2","pred":"source","obj":"mondo_disease"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":702,"end":708},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":790,"end":796},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":814,"end":819},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":1091,"end":1096},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9913"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9913"},{"id":"A3","pred":"db_id","subj":"T3","obj":"9606"},{"id":"A4","pred":"db_id","subj":"T4","obj":"9606"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"},{"subj":"T3","pred":"source","obj":"NCBITAXON"},{"subj":"T4","pred":"source","obj":"NCBITAXON"}]},{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":88,"end":92},"obj":"Body_part"},{"id":"T2","span":{"begin":97,"end":106},"obj":"Body_part"},{"id":"T3","span":{"begin":419,"end":423},"obj":"Body_part"},{"id":"T4","span":{"begin":428,"end":437},"obj":"Body_part"},{"id":"T5","span":{"begin":647,"end":651},"obj":"Body_part"},{"id":"T6","span":{"begin":656,"end":665},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000299"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000189"},{"id":"A3","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000299"},{"id":"A4","pred":"mat_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/MAT_0000189"},{"id":"A5","pred":"mat_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MAT_0000299"},{"id":"A6","pred":"mat_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/MAT_0000189"},{"subj":"T1","pred":"source","obj":"Anatomy-MAT"},{"subj":"T2","pred":"source","obj":"Anatomy-MAT"},{"subj":"T3","pred":"source","obj":"Anatomy-MAT"},{"subj":"T4","pred":"source","obj":"Anatomy-MAT"},{"subj":"T5","pred":"source","obj":"Anatomy-MAT"},{"subj":"T6","pred":"source","obj":"Anatomy-MAT"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":88,"end":92},"obj":"Body_part"},{"id":"T3","span":{"begin":97,"end":112},"obj":"Body_part"},{"id":"T4","span":{"begin":419,"end":423},"obj":"Body_part"},{"id":"T6","span":{"begin":428,"end":443},"obj":"Body_part"},{"id":"T7","span":{"begin":647,"end":651},"obj":"Body_part"},{"id":"T9","span":{"begin":656,"end":671},"obj":"Body_part"},{"id":"T10","span":{"begin":709,"end":713},"obj":"Body_part"},{"id":"T11","span":{"begin":797,"end":809},"obj":"Body_part"},{"id":"T12","span":{"begin":820,"end":831},"obj":"Body_part"},{"id":"T13","span":{"begin":1097,"end":1108},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0001474"},{"id":"A2","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0002481"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL_0000138"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0001474"},{"id":"A5","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0002481"},{"id":"A6","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/CL_0000138"},{"id":"A7","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/UBERON_0001474"},{"id":"A8","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/UBERON_0002481"},{"id":"A9","pred":"uberon_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/CL_0000138"},{"id":"A10","pred":"uberon_id","subj":"T10","obj":"http://purl.obolibrary.org/obo/UBERON_0001913"},{"id":"A11","pred":"uberon_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/CL_0000138"},{"id":"A12","pred":"uberon_id","subj":"T12","obj":"http://purl.obolibrary.org/obo/CL_0000062"},{"id":"A13","pred":"uberon_id","subj":"T13","obj":"http://purl.obolibrary.org/obo/CL_0000062"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T3","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T4","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T6","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T7","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T9","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T10","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T11","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T12","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T13","pred":"source","obj":"Anatomy-UBERON"}]},{"project":"Glycosmos15-CL","denotations":[{"id":"T1","span":{"begin":97,"end":112},"obj":"Cell"},{"id":"T2","span":{"begin":428,"end":443},"obj":"Cell"},{"id":"T3","span":{"begin":656,"end":671},"obj":"Cell"},{"id":"T4","span":{"begin":797,"end":809},"obj":"Cell"},{"id":"T5","span":{"begin":820,"end":831},"obj":"Cell"},{"id":"T6","span":{"begin":1097,"end":1108},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000138"},{"id":"A2","pred":"cl_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL:0000138"},{"id":"A3","pred":"cl_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL:0000138"},{"id":"A4","pred":"cl_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL:0000138"},{"id":"A5","pred":"cl_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CL:0000062"},{"id":"A6","pred":"cl_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/CL:0000062"},{"subj":"T1","pred":"source","obj":"Glycosmos15-CL"},{"subj":"T2","pred":"source","obj":"Glycosmos15-CL"},{"subj":"T3","pred":"source","obj":"Glycosmos15-CL"},{"subj":"T4","pred":"source","obj":"Glycosmos15-CL"},{"subj":"T5","pred":"source","obj":"Glycosmos15-CL"},{"subj":"T6","pred":"source","obj":"Glycosmos15-CL"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"GlyCosmos6-Glycan-Motif-Image","color":"#93d6ec","default":true},{"id":"Glycosmos6-MAT","color":"#ece793"},{"id":"sentences","color":"#cd93ec"},{"id":"GlyCosmos6-Glycan-Motif-Structure","color":"#93ecb3"},{"id":"GlyCosmos15-HP","color":"#ec9993"},{"id":"mondo_disease","color":"#93a6ec"},{"id":"NCBITAXON","color":"#c0ec93"},{"id":"Anatomy-MAT","color":"#ec93da"},{"id":"Anatomy-UBERON","color":"#93ece4"},{"id":"Glycosmos15-CL","color":"#ecca93"}]}]}}