PubMed:15979052 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/15979052","sourcedb":"PubMed","sourceid":"15979052","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/15979052","text":"The linkage between O-specific caryan and core region in the lipopolysaccharide of Burkholderia caryophylli is furnished by a primer monosaccharide.\nFrom the lipopolysaccharide (LPS) fraction of the plant-pathogenic bacterium Burkholderia caryophylli, the linkage between O-specific caryan and core region was characterised. The LPS fraction was first treated with 48% aqueous HF at 4 degrees C and successively with 1% acetic acid at 100 degrees C. A main oligosaccharide representing the carbohydrate backbone of the core region and a portion of the caryan (three unit of caryose) was isolated by high-performance anion-exchange chromatography. Compositional and methylation analyses, matrix-assisted laser desorption/ionisation mass spectrometry and 2D NMR spectroscopy identified the structure: [carbohydrate structure: see text]. The above residues are alpha-linked pyranose rings, if not stated otherwise. Hep is L-glycero-D-manno-heptose, Car is 4,8-cyclo-3,9-dideoxy-L-erythro-D-ido-nonose and Kdo is 3-deoxy-D-manno-oct-2-ulosonic acid. This finding indicates that QuiNAc residue is the primer monosaccharide, which connects the core oligosaccharide to caryan O-chain.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":148},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":149,"end":324},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":325,"end":449},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":450,"end":646},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":647,"end":834},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":835,"end":911},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":912,"end":1045},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1046,"end":1177},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":148},"obj":"Sentence"},{"id":"T2","span":{"begin":149,"end":324},"obj":"Sentence"},{"id":"T3","span":{"begin":325,"end":449},"obj":"Sentence"},{"id":"T4","span":{"begin":450,"end":646},"obj":"Sentence"},{"id":"T5","span":{"begin":647,"end":834},"obj":"Sentence"},{"id":"T6","span":{"begin":835,"end":911},"obj":"Sentence"},{"id":"T7","span":{"begin":912,"end":1045},"obj":"Sentence"},{"id":"T8","span":{"begin":1046,"end":1177},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"}]},{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":946,"end":949},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0011404"},{"subj":"T1","pred":"source","obj":"mondo_disease"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":83,"end":107},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":226,"end":250},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":946,"end":949},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"28094"},{"id":"A2","pred":"db_id","subj":"T2","obj":"28094"},{"id":"A3","pred":"db_id","subj":"T3","obj":"201850"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"},{"subj":"T3","pred":"source","obj":"NCBITAXON"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":503,"end":511},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0001130"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#ec9397","default":true},{"id":"mondo_disease","color":"#93b1ec"},{"id":"NCBITAXON","color":"#cbec93"},{"id":"Anatomy-UBERON","color":"#ec93e5"}]}]}}