PubMed:15864433 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/15864433","sourcedb":"PubMed","sourceid":"15864433","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/15864433","text":"Mutational studies on endo-beta-N-acetylglucosaminidase D which hydrolyzes core portion of asparagine-linked complex type oligosaccharides.\nEndo-beta-N-acetylglucosaminidase D (Endo D) produced by Streptococcus pneumoniae hydrolyzes the di-N-acetylchitobiose structure in the core of complex-type asparagine-linked oligosaccharides, and has a molecular weight of 180 kDa. A truncated Endo D of 102 kDa in which 134 N-terminal amino acids and 599 C-terminal amino acids were deleted, still retained the enzymatic activity. The truncated Endo D has specificity indistinguishable from the intact enzyme, and also acted on the core structure of asparagine-linked oligosaccharides attached to intact IgG. Because of its lower molecular weight, the truncated enzyme may be useful as a tool for protein deglycosylation. The entire region of the truncated Endo D had 32% sequence identity to endo- beta-N-acetylglucosaminidase BH (Endo BH) from Bacillus halodurans, which acted on high-mannose type oligosaccharides. Chimeric constructs of the truncated Endo D and Endo BH showed no activity. Glutamic acid 324 (E 324) in Endo D is conserved in Endo BH and Endo M, and is an essential amino acid in Endo M. Mutation of E324 abolished Endo D activity. The specificity of Endo D for complex type oligosaccharides is probably defined by multiple domains in the Endo D structure.","tracks":[{"project":"ngly1-sample8","denotations":[{"id":"T1","span":{"begin":788,"end":811},"obj":"GO:0006517"}],"attributes":[{"subj":"T1","pred":"source","obj":"ngly1-sample8"}]},{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":978,"end":985},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ"},{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"}]},{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":978,"end":985},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"}]},{"project":"NGLY1-deficiency","denotations":[{"id":"PD-NGLY1-deficiency-B_T1","span":{"begin":22,"end":55},"obj":"hgnc:24622"},{"id":"PD-NGLY1-deficiency-B_T2","span":{"begin":140,"end":173},"obj":"hgnc:24622"},{"id":"PD-NGLY1-deficiency-B_T3","span":{"begin":884,"end":918},"obj":"hgnc:24622"}],"namespaces":[{"prefix":"hgnc","uri":"https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:"},{"prefix":"omim","uri":"https://www.omim.org/entry/"},{"prefix":"chem","uri":"https://pubchem.ncbi.nlm.nih.gov/compound/"}],"attributes":[{"subj":"PD-NGLY1-deficiency-B_T1","pred":"source","obj":"NGLY1-deficiency"},{"subj":"PD-NGLY1-deficiency-B_T2","pred":"source","obj":"NGLY1-deficiency"},{"subj":"PD-NGLY1-deficiency-B_T3","pred":"source","obj":"NGLY1-deficiency"}]},{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":139},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":140,"end":371},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":372,"end":521},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":522,"end":699},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":700,"end":812},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":813,"end":1008},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":1009,"end":1084},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1085,"end":1198},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1199,"end":1242},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":1243,"end":1367},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":139},"obj":"Sentence"},{"id":"T2","span":{"begin":140,"end":371},"obj":"Sentence"},{"id":"T3","span":{"begin":372,"end":521},"obj":"Sentence"},{"id":"T4","span":{"begin":522,"end":699},"obj":"Sentence"},{"id":"T5","span":{"begin":700,"end":812},"obj":"Sentence"},{"id":"T6","span":{"begin":813,"end":1008},"obj":"Sentence"},{"id":"T7","span":{"begin":1009,"end":1084},"obj":"Sentence"},{"id":"T8","span":{"begin":1085,"end":1198},"obj":"Sentence"},{"id":"T9","span":{"begin":1199,"end":1242},"obj":"Sentence"},{"id":"T10","span":{"begin":1243,"end":1367},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T9","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T10","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"}]},{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":197,"end":221},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0005972"},{"subj":"T1","pred":"source","obj":"mondo_disease"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":197,"end":221},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":937,"end":945},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"1313"},{"id":"A2","pred":"db_id","subj":"T2","obj":"1386"},{"id":"A3","pred":"db_id","subj":"T2","obj":"55087"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"}]},{"project":"GlyCosmos15-HP","denotations":[{"id":"T1","span":{"begin":211,"end":221},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0002090"},{"subj":"T1","pred":"source","obj":"GlyCosmos15-HP"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"ngly1-sample8","color":"#acec93","default":true},{"id":"GlyCosmos6-Glycan-Motif-Image","color":"#ec93c6"},{"id":"GlyCosmos6-Glycan-Motif-Structure","color":"#93e0ec"},{"id":"NGLY1-deficiency","color":"#ecdd93"},{"id":"sentences","color":"#c393ec"},{"id":"mondo_disease","color":"#93eca9"},{"id":"NCBITAXON","color":"#ec9396"},{"id":"GlyCosmos15-HP","color":"#93b0ec"}]}]}}