PubMed:15833836 JSON TXT

Annnotations TAB JSON ListView MergeView

DisGeNET5_gene_disease

DisGeNet-2017-sample

{"project":"DisGeNet-2017-sample","denotations":[{"id":"T584","span":{"begin":1625,"end":1627},"obj":"gene:2099"},{"id":"T585","span":{"begin":1735,"end":1748},"obj":"disease:C0006142"},{"id":"T588","span":{"begin":18,"end":24},"obj":"gene:5458"},{"id":"T589","span":{"begin":86,"end":99},"obj":"disease:C0006142"},{"id":"T590","span":{"begin":76,"end":82},"obj":"gene:3315"},{"id":"T591","span":{"begin":155,"end":161},"obj":"gene:3315"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T584","obj":"T585"},{"id":"R2","pred":"associated_with","subj":"T584","obj":"T585"},{"id":"R1","pred":"associated_with","subj":"T588","obj":"T589"},{"id":"R2","pred":"associated_with","subj":"T588","obj":"T589"},{"id":"R3","pred":"associated_with","subj":"T590","obj":"T589"},{"id":"R4","pred":"associated_with","subj":"T590","obj":"T589"},{"id":"R5","pred":"associated_with","subj":"T590","obj":"T589"},{"id":"R6","pred":"associated_with","subj":"T590","obj":"T589"},{"id":"R7","pred":"associated_with","subj":"T590","obj":"T589"},{"id":"R8","pred":"associated_with","subj":"T590","obj":"T589"},{"id":"R9","pred":"associated_with","subj":"T591","obj":"T589"},{"id":"R10","pred":"associated_with","subj":"T591","obj":"T589"},{"id":"R11","pred":"associated_with","subj":"T591","obj":"T589"},{"id":"R12","pred":"associated_with","subj":"T591","obj":"T589"},{"id":"R13","pred":"associated_with","subj":"T591","obj":"T589"},{"id":"R14","pred":"associated_with","subj":"T591","obj":"T589"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"text":"Expression of the Brn-3b transcription factor correlates with expression of HSP-27 in breast cancer biopsies and is required for maximal activation of the HSP-27 promoter.\nIn breast cancer, overexpression of the small heat shock protein, HSP-27, is associated with increased anchorage-independent growth, increased invasiveness, and resistance to chemotherapeutic drugs and is associated with poor prognosis and reduced disease-free survival. Therefore, factors that increase the expression of HSP-27 in breast cancer are likely to affect the prognosis and outcome of treatment. In this study, we show a strong correlation between elevated levels of the Brn-3b POU transcription factor and high levels of HSP-27 protein in manipulated MCF-7 breast cancer cells as well as in human breast biopsies. Conversely, HSP-27 is decreased on loss of Brn-3b. In cotransfection assays, Brn-3b can strongly transactivate the HSP-27 promoter, supporting a role for direct regulation of HSP-27 expression. Brn-3b also cooperates with the estrogen receptor (ER) to facilitate maximal stimulation of the HSP-27 promoter, with significantly enhanced activity of this promoter observed on coexpression of Brn-3b and ER compared with either alone. RNA interference and site-directed mutagenesis support the requirement for the Brn-3b binding site on the HSP-27 promoter, which facilitates maximal transactivation either alone or on interaction with the ER. Chromatin immunoprecipitation provides evidence for association of Brn-3b with the HSP-27 promoter in the intact cell. Thus, Brn-3b can, directly and indirectly (via interaction with the ER), activate HSP-27 expression, and this may represent one mechanism by which Brn-3b mediates its effects in breast cancer cells."}

UBERON-AE

{"project":"UBERON-AE","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":86,"end":92},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T2","span":{"begin":175,"end":181},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T3","span":{"begin":504,"end":510},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T4","span":{"begin":741,"end":747},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T5","span":{"begin":781,"end":787},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T6","span":{"begin":1735,"end":1741},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"}],"text":"Expression of the Brn-3b transcription factor correlates with expression of HSP-27 in breast cancer biopsies and is required for maximal activation of the HSP-27 promoter.\nIn breast cancer, overexpression of the small heat shock protein, HSP-27, is associated with increased anchorage-independent growth, increased invasiveness, and resistance to chemotherapeutic drugs and is associated with poor prognosis and reduced disease-free survival. Therefore, factors that increase the expression of HSP-27 in breast cancer are likely to affect the prognosis and outcome of treatment. In this study, we show a strong correlation between elevated levels of the Brn-3b POU transcription factor and high levels of HSP-27 protein in manipulated MCF-7 breast cancer cells as well as in human breast biopsies. Conversely, HSP-27 is decreased on loss of Brn-3b. In cotransfection assays, Brn-3b can strongly transactivate the HSP-27 promoter, supporting a role for direct regulation of HSP-27 expression. Brn-3b also cooperates with the estrogen receptor (ER) to facilitate maximal stimulation of the HSP-27 promoter, with significantly enhanced activity of this promoter observed on coexpression of Brn-3b and ER compared with either alone. RNA interference and site-directed mutagenesis support the requirement for the Brn-3b binding site on the HSP-27 promoter, which facilitates maximal transactivation either alone or on interaction with the ER. Chromatin immunoprecipitation provides evidence for association of Brn-3b with the HSP-27 promoter in the intact cell. Thus, Brn-3b can, directly and indirectly (via interaction with the ER), activate HSP-27 expression, and this may represent one mechanism by which Brn-3b mediates its effects in breast cancer cells."}

sentences

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":171},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":172,"end":442},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":443,"end":578},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":579,"end":797},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":798,"end":848},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":849,"end":991},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":992,"end":1228},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1229,"end":1437},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1438,"end":1556},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":1557,"end":1755},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":171},"obj":"Sentence"},{"id":"T2","span":{"begin":172,"end":442},"obj":"Sentence"},{"id":"T3","span":{"begin":443,"end":578},"obj":"Sentence"},{"id":"T4","span":{"begin":579,"end":797},"obj":"Sentence"},{"id":"T5","span":{"begin":798,"end":848},"obj":"Sentence"},{"id":"T6","span":{"begin":849,"end":991},"obj":"Sentence"},{"id":"T7","span":{"begin":992,"end":1228},"obj":"Sentence"},{"id":"T8","span":{"begin":1229,"end":1437},"obj":"Sentence"},{"id":"T9","span":{"begin":1438,"end":1556},"obj":"Sentence"},{"id":"T10","span":{"begin":1557,"end":1755},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Expression of the Brn-3b transcription factor correlates with expression of HSP-27 in breast cancer biopsies and is required for maximal activation of the HSP-27 promoter.\nIn breast cancer, overexpression of the small heat shock protein, HSP-27, is associated with increased anchorage-independent growth, increased invasiveness, and resistance to chemotherapeutic drugs and is associated with poor prognosis and reduced disease-free survival. Therefore, factors that increase the expression of HSP-27 in breast cancer are likely to affect the prognosis and outcome of treatment. In this study, we show a strong correlation between elevated levels of the Brn-3b POU transcription factor and high levels of HSP-27 protein in manipulated MCF-7 breast cancer cells as well as in human breast biopsies. Conversely, HSP-27 is decreased on loss of Brn-3b. In cotransfection assays, Brn-3b can strongly transactivate the HSP-27 promoter, supporting a role for direct regulation of HSP-27 expression. Brn-3b also cooperates with the estrogen receptor (ER) to facilitate maximal stimulation of the HSP-27 promoter, with significantly enhanced activity of this promoter observed on coexpression of Brn-3b and ER compared with either alone. RNA interference and site-directed mutagenesis support the requirement for the Brn-3b binding site on the HSP-27 promoter, which facilitates maximal transactivation either alone or on interaction with the ER. Chromatin immunoprecipitation provides evidence for association of Brn-3b with the HSP-27 promoter in the intact cell. Thus, Brn-3b can, directly and indirectly (via interaction with the ER), activate HSP-27 expression, and this may represent one mechanism by which Brn-3b mediates its effects in breast cancer cells."}

performance-test

{"project":"performance-test","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":86,"end":92},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T2","span":{"begin":175,"end":181},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T3","span":{"begin":504,"end":510},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T4","span":{"begin":741,"end":747},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T5","span":{"begin":781,"end":787},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T6","span":{"begin":1735,"end":1741},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"}],"text":"Expression of the Brn-3b transcription factor correlates with expression of HSP-27 in breast cancer biopsies and is required for maximal activation of the HSP-27 promoter.\nIn breast cancer, overexpression of the small heat shock protein, HSP-27, is associated with increased anchorage-independent growth, increased invasiveness, and resistance to chemotherapeutic drugs and is associated with poor prognosis and reduced disease-free survival. Therefore, factors that increase the expression of HSP-27 in breast cancer are likely to affect the prognosis and outcome of treatment. In this study, we show a strong correlation between elevated levels of the Brn-3b POU transcription factor and high levels of HSP-27 protein in manipulated MCF-7 breast cancer cells as well as in human breast biopsies. Conversely, HSP-27 is decreased on loss of Brn-3b. In cotransfection assays, Brn-3b can strongly transactivate the HSP-27 promoter, supporting a role for direct regulation of HSP-27 expression. Brn-3b also cooperates with the estrogen receptor (ER) to facilitate maximal stimulation of the HSP-27 promoter, with significantly enhanced activity of this promoter observed on coexpression of Brn-3b and ER compared with either alone. RNA interference and site-directed mutagenesis support the requirement for the Brn-3b binding site on the HSP-27 promoter, which facilitates maximal transactivation either alone or on interaction with the ER. Chromatin immunoprecipitation provides evidence for association of Brn-3b with the HSP-27 promoter in the intact cell. Thus, Brn-3b can, directly and indirectly (via interaction with the ER), activate HSP-27 expression, and this may represent one mechanism by which Brn-3b mediates its effects in breast cancer cells."}

DisGeNET

{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":1563,"end":1569},"obj":"gene:5458"},{"id":"T1","span":{"begin":1735,"end":1748},"obj":"disease:C0006142"},{"id":"T2","span":{"begin":1563,"end":1569},"obj":"gene:5458"},{"id":"T3","span":{"begin":1735,"end":1748},"obj":"disease:C0678222"},{"id":"T4","span":{"begin":1625,"end":1627},"obj":"gene:2099"},{"id":"T5","span":{"begin":1735,"end":1748},"obj":"disease:C0006142"},{"id":"T6","span":{"begin":1625,"end":1627},"obj":"gene:2099"},{"id":"T7","span":{"begin":1735,"end":1748},"obj":"disease:C0678222"},{"id":"T8","span":{"begin":1704,"end":1710},"obj":"gene:5458"},{"id":"T9","span":{"begin":1735,"end":1748},"obj":"disease:C0006142"},{"id":"T10","span":{"begin":1704,"end":1710},"obj":"gene:5458"},{"id":"T11","span":{"begin":1735,"end":1748},"obj":"disease:C0678222"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"},{"id":"R3","pred":"associated_with","subj":"T4","obj":"T5"},{"id":"R4","pred":"associated_with","subj":"T6","obj":"T7"},{"id":"R5","pred":"associated_with","subj":"T8","obj":"T9"},{"id":"R6","pred":"associated_with","subj":"T10","obj":"T11"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"text":"Expression of the Brn-3b transcription factor correlates with expression of HSP-27 in breast cancer biopsies and is required for maximal activation of the HSP-27 promoter.\nIn breast cancer, overexpression of the small heat shock protein, HSP-27, is associated with increased anchorage-independent growth, increased invasiveness, and resistance to chemotherapeutic drugs and is associated with poor prognosis and reduced disease-free survival. Therefore, factors that increase the expression of HSP-27 in breast cancer are likely to affect the prognosis and outcome of treatment. In this study, we show a strong correlation between elevated levels of the Brn-3b POU transcription factor and high levels of HSP-27 protein in manipulated MCF-7 breast cancer cells as well as in human breast biopsies. Conversely, HSP-27 is decreased on loss of Brn-3b. In cotransfection assays, Brn-3b can strongly transactivate the HSP-27 promoter, supporting a role for direct regulation of HSP-27 expression. Brn-3b also cooperates with the estrogen receptor (ER) to facilitate maximal stimulation of the HSP-27 promoter, with significantly enhanced activity of this promoter observed on coexpression of Brn-3b and ER compared with either alone. RNA interference and site-directed mutagenesis support the requirement for the Brn-3b binding site on the HSP-27 promoter, which facilitates maximal transactivation either alone or on interaction with the ER. Chromatin immunoprecipitation provides evidence for association of Brn-3b with the HSP-27 promoter in the intact cell. Thus, Brn-3b can, directly and indirectly (via interaction with the ER), activate HSP-27 expression, and this may represent one mechanism by which Brn-3b mediates its effects in breast cancer cells."}