PubMed:15797649 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/15797649","sourcedb":"PubMed","sourceid":"15797649","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/15797649","text":"Maternal autoantibodies from preeclamptic patients activate angiotensin receptors on human mesangial cells and induce interleukin-6 and plasminogen activator inhibitor-1 secretion.\nBACKGROUND: Preeclampsia affects 3-5% of all pregnancies. It is a major cause of maternal and fetal morbidity and mortality. Recent studies demonstrate that autoantibodies against the angiotensin II type 1 (AT(1)) receptor are present in the serum of preeclamptic patients. In this study, we investigated the role of AT(1) receptor-agonistic autoantibody (AT1-AA) regarding interleukin-6 (IL-6) and plasminogen activator inhibitor-1 (Pai-1) secretion in human mesangial cells.\nMETHODS: The study included ten patients: five severely preeclamptic and five normotensive pregnant women. Immunoglobulin-G (IgG) was purified from each individual. The presence of AT1-AA was determined based on its ability to stimulate an increase in the contraction rate of rat neonatal cardiomyocytes. Primary human mesangial cells were chosen to study IgG-induced secretion of IL-6 and Pai-1. Losartan and epitope peptides were used to determine whether AT1-AA interaction with AT(1) receptor was associated with stimulation of IL-6 and Pai-1 secretion and was mediated through AT(1) receptor activation.\nRESULTS: The IgG from preeclamptic patients stimulated an increased contraction rate in rat neonatal cardiomyocytes. The IgG from preeclamptic patients induced the AT(1) receptor-specific secretion of IL-6 and Pai-1 from human mesangial cells at a significantly higher level than that achieved with IgG from normotensive patients. Competition with an epitope peptide suggested that the AT(1) receptor was stimulated by AT1-AA.\nCONCLUSIONS: Our findings suggest that a maternal autoantibody with the ability to activate AT(1) receptors may account for the development of renal damage seen in preeclamptic patients.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":180},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":181,"end":192},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":193,"end":238},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":239,"end":305},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":306,"end":454},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":455,"end":657},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":658,"end":666},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":667,"end":764},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":765,"end":822},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":823,"end":962},"obj":"Sentence"},{"id":"TextSentencer_T11","span":{"begin":963,"end":1054},"obj":"Sentence"},{"id":"TextSentencer_T12","span":{"begin":1055,"end":1266},"obj":"Sentence"},{"id":"TextSentencer_T13","span":{"begin":1267,"end":1275},"obj":"Sentence"},{"id":"TextSentencer_T14","span":{"begin":1276,"end":1383},"obj":"Sentence"},{"id":"TextSentencer_T15","span":{"begin":1384,"end":1597},"obj":"Sentence"},{"id":"TextSentencer_T16","span":{"begin":1598,"end":1693},"obj":"Sentence"},{"id":"TextSentencer_T17","span":{"begin":1694,"end":1706},"obj":"Sentence"},{"id":"TextSentencer_T18","span":{"begin":1707,"end":1880},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":180},"obj":"Sentence"},{"id":"T2","span":{"begin":181,"end":192},"obj":"Sentence"},{"id":"T3","span":{"begin":193,"end":238},"obj":"Sentence"},{"id":"T4","span":{"begin":239,"end":305},"obj":"Sentence"},{"id":"T5","span":{"begin":306,"end":454},"obj":"Sentence"},{"id":"T6","span":{"begin":455,"end":657},"obj":"Sentence"},{"id":"T7","span":{"begin":658,"end":666},"obj":"Sentence"},{"id":"T8","span":{"begin":667,"end":764},"obj":"Sentence"},{"id":"T9","span":{"begin":765,"end":822},"obj":"Sentence"},{"id":"T10","span":{"begin":823,"end":962},"obj":"Sentence"},{"id":"T11","span":{"begin":963,"end":1054},"obj":"Sentence"},{"id":"T12","span":{"begin":1055,"end":1266},"obj":"Sentence"},{"id":"T13","span":{"begin":1267,"end":1275},"obj":"Sentence"},{"id":"T14","span":{"begin":1276,"end":1383},"obj":"Sentence"},{"id":"T15","span":{"begin":1384,"end":1597},"obj":"Sentence"},{"id":"T16","span":{"begin":1598,"end":1693},"obj":"Sentence"},{"id":"T17","span":{"begin":1694,"end":1706},"obj":"Sentence"},{"id":"T18","span":{"begin":1707,"end":1880},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T9","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T10","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T11","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T12","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T13","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T14","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T15","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T16","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T17","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T18","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"},{"subj":"T11","pred":"source","obj":"sentences"},{"subj":"T12","pred":"source","obj":"sentences"},{"subj":"T13","pred":"source","obj":"sentences"},{"subj":"T14","pred":"source","obj":"sentences"},{"subj":"T15","pred":"source","obj":"sentences"},{"subj":"T16","pred":"source","obj":"sentences"},{"subj":"T17","pred":"source","obj":"sentences"},{"subj":"T18","pred":"source","obj":"sentences"}]},{"project":"Preeclampsia-compare","denotations":[{"id":"PD-Preeclampsia-B_T1","span":{"begin":29,"end":41},"obj":"ORPHA:275555"},{"id":"PD-Preeclampsia-B_T2","span":{"begin":193,"end":205},"obj":"ORPHA:275555"},{"id":"PD-Preeclampsia-B_T3","span":{"begin":432,"end":444},"obj":"ORPHA:275555"},{"id":"PD-Preeclampsia-B_T4","span":{"begin":714,"end":726},"obj":"ORPHA:275555"},{"id":"PD-Preeclampsia-B_T5","span":{"begin":1289,"end":1301},"obj":"ORPHA:275555"},{"id":"PD-Preeclampsia-B_T6","span":{"begin":1397,"end":1409},"obj":"ORPHA:275555"},{"id":"PD-Preeclampsia-B_T7","span":{"begin":1858,"end":1870},"obj":"ORPHA:275555"}],"namespaces":[{"prefix":"ORPHA","uri":"www.orpha.net/consor/cgi-bin/OC_Exp.php?lng=EN\u0026Expert="}],"attributes":[{"subj":"PD-Preeclampsia-B_T1","pred":"source","obj":"Preeclampsia-compare"},{"subj":"PD-Preeclampsia-B_T2","pred":"source","obj":"Preeclampsia-compare"},{"subj":"PD-Preeclampsia-B_T3","pred":"source","obj":"Preeclampsia-compare"},{"subj":"PD-Preeclampsia-B_T4","pred":"source","obj":"Preeclampsia-compare"},{"subj":"PD-Preeclampsia-B_T5","pred":"source","obj":"Preeclampsia-compare"},{"subj":"PD-Preeclampsia-B_T6","pred":"source","obj":"Preeclampsia-compare"},{"subj":"PD-Preeclampsia-B_T7","pred":"source","obj":"Preeclampsia-compare"}]},{"project":"Preeclampsia","denotations":[{"id":"PD-Preeclampsia-B_T1","span":{"begin":29,"end":50},"obj":"ORPHA:275555"},{"id":"PD-Preeclampsia-B_T2","span":{"begin":193,"end":205},"obj":"ORPHA:275555"},{"id":"PD-Preeclampsia-B_T3","span":{"begin":432,"end":453},"obj":"ORPHA:275555"},{"id":"PD-Preeclampsia-B_T4","span":{"begin":1289,"end":1310},"obj":"ORPHA:275555"},{"id":"PD-Preeclampsia-B_T5","span":{"begin":1397,"end":1418},"obj":"ORPHA:275555"},{"id":"PD-Preeclampsia-B_T6","span":{"begin":1858,"end":1879},"obj":"ORPHA:275555"}],"namespaces":[{"prefix":"ORPHA","uri":"www.orpha.net/consor/cgi-bin/OC_Exp.php?lng=EN\u0026Expert="}],"attributes":[{"subj":"PD-Preeclampsia-B_T1","pred":"source","obj":"Preeclampsia"},{"subj":"PD-Preeclampsia-B_T2","pred":"source","obj":"Preeclampsia"},{"subj":"PD-Preeclampsia-B_T3","pred":"source","obj":"Preeclampsia"},{"subj":"PD-Preeclampsia-B_T4","pred":"source","obj":"Preeclampsia"},{"subj":"PD-Preeclampsia-B_T5","pred":"source","obj":"Preeclampsia"},{"subj":"PD-Preeclampsia-B_T6","pred":"source","obj":"Preeclampsia"}]},{"project":"performance-test","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":423,"end":428},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"attributes":[{"subj":"PD-UBERON-AE-B_T1","pred":"source","obj":"performance-test"}]},{"project":"UBERON-AE","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":423,"end":428},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"attributes":[{"subj":"PD-UBERON-AE-B_T1","pred":"source","obj":"UBERON-AE"}]},{"project":"preeclampsia_genes","denotations":[{"id":"PD-PreeclampsiaGenes-B_T1","span":{"begin":118,"end":131},"obj":"HGNC:IL6"},{"id":"PD-PreeclampsiaGenes-B_T2","span":{"begin":555,"end":568},"obj":"HGNC:IL6"}],"attributes":[{"subj":"PD-PreeclampsiaGenes-B_T1","pred":"source","obj":"preeclampsia_genes"},{"subj":"PD-PreeclampsiaGenes-B_T2","pred":"source","obj":"preeclampsia_genes"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#93ecb9","default":true},{"id":"Preeclampsia-compare","color":"#ec9f93"},{"id":"Preeclampsia","color":"#93a1ec"},{"id":"performance-test","color":"#bbec93"},{"id":"UBERON-AE","color":"#ec93d5"},{"id":"preeclampsia_genes","color":"#93ece9"}]}]}}