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PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 112-322 OBJECTIVE denotes Corneal stromal myofibroblasts express the platelet-activating factor (PAF) receptor, but its role is unclear. In the present study, the effect of PAF on induction of metalloproteinases (MMPs) was investigated.
T2 332-967 METHODS denotes Rabbit corneal myofibroblasts were identified by immunodetection of alpha-smooth muscle (alpha-SM)-actin. MT1-MMP, MMP-2, MMP-9, and tissue inhibitor of matrix metalloproteinase (TIMP)-2 were detected by immunofluorescence. Cells were treated with 100 nM cPAF, with or without the PAF antagonist BN 50730 or the furin inhibitor nona-D-arg-NH(2). Gene-expression levels for furin, urokinase plasminogen activator, MMP-2, MMP-9, MT1-MMP, and TIMP-2 were determined by real-time PCR. Protein expression was assessed by Western blot. MMP-2 and -9 activity was determined by gelatin zymography. Active MT1-MMP levels were measured by ELISA.
T3 977-1516 RESULTS denotes cPAF triggered significantly increased MT1-MMP, MMP-2, MMP-9, and TIMP-2 mRNA expression, followed by increased active MT1-MMP protein expression at 12 hours, whereas TIMP-2 protein increased at 24 hours. PAF also induced furin gene expression, followed by increased protein expression. Nona-D-arg-NH(2) blocked cPAF induction of MT1-MMP activity. PAF-treated myofibroblasts showed increased active MMP-9 protein, but unchanged MMP-2 activity. Pretreatment with BN 50730 blocked PAF-induced transcription and translation of these proteins.
T4 1530-2101 CONCLUSIONS denotes PAF, through a receptor-mediated mechanism, induces a specific pattern of furin, MMP, and TIMP-2 expression in corneal myofibroblasts. MMP-2 activity was unchanged by PAF treatment. These results suggest that in response to the inflammatory mediator PAF, induction of MT1-MMP is independent of MMP-2 activity in corneal myofibroblasts. Thus, PAF-mediated changes in extracellular matrix composition surrounding the myofibroblasts could be important in regulating the corneal scarring process. Moreover, PAF antagonists could be useful in maintaining corneal transparency.

c_corpus

Id Subject Object Predicate Lexical cue
T1 0-3 PR:Q6RIA2 denotes PAF
T2 0-3 PR:Q15004 denotes PAF
T5 0-3 PR:000012225 denotes PAF
T7 0-3 PR:Q9CQX4 denotes PAF
T6 0-3 CVCL_XY57 denotes PAF
T3 0-3 CHEBI:52450 denotes PAF
T4 0-3 CHEBI:44811 denotes PAF
T8 12-17 P09958 denotes furin
T9 12-17 P23188 denotes furin
T10 12-17 PR:000003137 denotes furin
T12 12-17 PR:P09958 denotes furin
T13 12-17 Q28193 denotes furin
T14 12-17 PR:P23188 denotes furin
T15 12-17 P23377 denotes furin
T16 12-17 PR:P23377 denotes furin
T11 12-17 D045683 denotes furin
T39 22-25 CVCL_2501 denotes MT1
T24 22-25 GO:0047152 denotes MT1
T33 22-25 GO:0043791 denotes MT1
T42 22-25 GO:0043834 denotes MT1
T58 22-29 Q9XT90 denotes MT1-MMP
T59 22-29 P50281 denotes MT1-MMP
T60 22-29 Q9GLE4 denotes MT1-MMP
T61 22-29 PR:000010474 denotes MT1-MMP
T62 22-29 Q10739 denotes MT1-MMP
T63 22-29 Q95220 denotes MT1-MMP
T65 22-29 P53690 denotes MT1-MMP
T64 22-29 D053511 denotes MT1-MMP
T68 26-29 GO:0004235 denotes MMP
T71 59-62 CHEBI:340824 denotes MMP
T74 59-62 CHEBI:59761 denotes MMP
T75 59-64 PR:P08253 denotes MMP-2
T76 59-64 Q9GLE5 denotes MMP-2
T77 59-64 P08253 denotes MMP-2
T78 59-64 Q90611 denotes MMP-2
T80 59-64 PR:Q8MPP3 denotes MMP-2
T81 59-64 P33436 denotes MMP-2
T82 59-64 PR:Q90611 denotes MMP-2
T83 59-64 PR:P33436 denotes MMP-2
T84 59-64 PR:000010479 denotes MMP-2
T85 59-64 PR:P33434 denotes MMP-2
T86 59-64 P33434 denotes MMP-2
T87 59-64 P50757 denotes MMP-2
T79 59-64 GO:0004228 denotes MMP-2
T88 155-163 CL:0000233 denotes platelet
T89 155-181 CHEBI:36707 denotes platelet-activating factor
T90 155-181 CHEBI:52450 denotes platelet-activating factor
T91 155-181 CHEBI:44811 denotes platelet-activating factor
T92 155-181 CHEBI:78103 denotes platelet-activating factor
T93 155-181 D010972 denotes platelet-activating factor
T94 155-181 D010972 denotes platelet-activating factor
T100 183-186 CVCL_XY57 denotes PAF
T97 183-186 CHEBI:52450 denotes PAF
T98 183-186 CHEBI:44811 denotes PAF
T102 183-196 PR:000050017 denotes PAF) receptor
T103 259-262 PR:Q6RIA2 denotes PAF
T104 259-262 PR:Q15004 denotes PAF
T107 259-262 PR:000012225 denotes PAF
T109 259-262 PR:Q9CQX4 denotes PAF
T108 259-262 CVCL_XY57 denotes PAF
T105 259-262 CHEBI:52450 denotes PAF
T106 259-262 CHEBI:44811 denotes PAF
T110 279-297 D045726 denotes metalloproteinases
T111 299-303 D020782 denotes MMPs
T113 332-338 813859 denotes Rabbit
T112 332-338 D011817 denotes Rabbit
T114 332-338 9986 denotes Rabbit
T115 400-405 CHEBI:30216 denotes alpha
T116 406-419 UBERON:0001135 denotes smooth muscle
T117 406-419 UBERON:0000953 denotes smooth muscle
T120 421-426 CHEBI:30216 denotes alpha
T121 431-436 P53491 denotes actin
T122 431-436 P45520 denotes actin
T123 431-436 O00937 denotes actin
T124 431-436 Q05214 denotes actin
T125 431-436 P13363 denotes actin
T126 431-436 Q39596 denotes actin
T127 431-436 P30161 denotes actin
T128 431-436 P53476 denotes actin
T129 431-436 P51775 denotes actin
T130 431-436 P60011 denotes actin
T131 431-436 Q9P4D1 denotes actin
T132 431-436 P50138 denotes actin
T133 431-436 O65314 denotes actin
T134 431-436 P26183 denotes actin
T135 431-436 Q75D00 denotes actin
T136 431-436 P53502 denotes actin
T137 431-436 P11426 denotes actin
T138 431-436 P80709 denotes actin
T139 431-436 Q8X119 denotes actin
T140 431-436 Q6TCF2 denotes actin
T141 431-436 Q99023 denotes actin
T142 431-436 A5DQP9 denotes actin
T143 431-436 P60009 denotes actin
T144 431-436 O65315 denotes actin
T145 431-436 P26182 denotes actin
T146 431-436 Q9UVF3 denotes actin
T147 431-436 Q2U7A3 denotes actin
T148 431-436 Q92192 denotes actin
T149 431-436 Q9UVZ8 denotes actin
T150 431-436 P53689 denotes actin
T151 431-436 P68555 denotes actin
T152 431-436 P53455 denotes actin
T153 431-436 O13419 denotes actin
T154 431-436 P60010 denotes actin
T155 431-436 Q39758 denotes actin
T156 431-436 P81085 denotes actin
T157 431-436 P48465 denotes actin
T158 431-436 P78711 denotes actin
T159 431-436 P17128 denotes actin
T160 431-436 P91754 denotes actin
T161 431-436 Q24733 denotes actin
T162 431-436 P14235 denotes actin
T163 431-436 Q92193 denotes actin
T164 431-436 Q11212 denotes actin
T165 431-436 P45521 denotes actin
T166 431-436 P53477 denotes actin
T167 431-436 P53499 denotes actin
T168 431-436 P24902 denotes actin
T169 431-436 Q8SWN8 denotes actin
T170 431-436 O17320 denotes actin
T171 431-436 P10365 denotes actin
T173 431-436 O16808 denotes actin
T174 431-436 P53498 denotes actin
T175 431-436 Q9UVX4 denotes actin
T176 431-436 O81221 denotes actin
T177 431-436 P20904 denotes actin
T178 431-436 O65316 denotes actin
T179 431-436 O74258 denotes actin
T180 431-436 P53500 denotes actin
T181 431-436 P10989 denotes actin
T182 431-436 P90689 denotes actin
T172 431-436 D000199 denotes actin
T205 438-441 CVCL_2501 denotes MT1
T190 438-441 GO:0047152 denotes MT1
T199 438-441 GO:0043791 denotes MT1
T208 438-441 GO:0043834 denotes MT1
T224 438-445 Q9XT90 denotes MT1-MMP
T225 438-445 P50281 denotes MT1-MMP
T226 438-445 Q9GLE4 denotes MT1-MMP
T227 438-445 PR:000010474 denotes MT1-MMP
T228 438-445 Q10739 denotes MT1-MMP
T229 438-445 Q95220 denotes MT1-MMP
T231 438-445 P53690 denotes MT1-MMP
T230 438-445 D053511 denotes MT1-MMP
T234 442-445 GO:0004235 denotes MMP
T237 447-450 CHEBI:340824 denotes MMP
T240 447-450 CHEBI:59761 denotes MMP
T241 447-452 PR:P08253 denotes MMP-2
T242 447-452 Q9GLE5 denotes MMP-2
T243 447-452 P08253 denotes MMP-2
T244 447-452 Q90611 denotes MMP-2
T246 447-452 PR:Q8MPP3 denotes MMP-2
T247 447-452 P33436 denotes MMP-2
T248 447-452 PR:Q90611 denotes MMP-2
T249 447-452 PR:P33436 denotes MMP-2
T250 447-452 PR:000010479 denotes MMP-2
T251 447-452 PR:P33434 denotes MMP-2
T252 447-452 P33434 denotes MMP-2
T253 447-452 P50757 denotes MMP-2
T245 447-452 GO:0004228 denotes MMP-2
T254 451-457 PR:O04529 denotes 2, MMP
T255 454-457 CHEBI:340824 denotes MMP
T258 454-457 CHEBI:59761 denotes MMP
T259 454-459 PR:P50282 denotes MMP-9
T260 454-459 PR:P14780 denotes MMP-9
T261 454-459 P14780 denotes MMP-9
T262 454-459 PR:P41245 denotes MMP-9
T263 454-459 P41246 denotes MMP-9
T264 454-459 P41245 denotes MMP-9
T265 454-459 PR:P52176 denotes MMP-9
T266 454-459 P50282 denotes MMP-9
T268 454-459 PR:000010491 denotes MMP-9
T269 454-459 P52176 denotes MMP-9
T270 454-459 O18733 denotes MMP-9
T267 454-459 GO:0004229 denotes MMP-9
T271 465-471 UBERON:0000479 denotes tissue
T272 472-481 CHEBI:35222 denotes inhibitor
T273 485-491 UBERON:0027368 denotes matrix
T278 511-518 PR:P16035 denotes TIMP)-2
T279 511-518 PR:000016353 denotes TIMP)-2
T280 511-518 O42146 denotes TIMP)-2
T281 511-518 Q9TRZ7 denotes TIMP)-2
T282 511-518 Q9TTY1 denotes TIMP)-2
T283 511-518 Q60453 denotes TIMP)-2
T284 511-518 P16035 denotes TIMP)-2
T286 511-518 P25785 denotes TIMP)-2
T287 511-518 P16368 denotes TIMP)-2
T288 511-518 O77717 denotes TIMP)-2
T289 511-518 PR:P25785 denotes TIMP)-2
T290 511-518 P30121 denotes TIMP)-2
T291 511-518 Q9WUC6 denotes TIMP)-2
T292 511-518 PR:P30121 denotes TIMP)-2
T293 511-518 PR:O42146 denotes TIMP)-2
T285 511-518 D019716 denotes TIMP)-2
T294 587-591 C083283 denotes cPAF
T295 587-591 C083283 denotes cPAF
T296 613-616 PR:Q6RIA2 denotes PAF
T297 613-616 PR:Q15004 denotes PAF
T300 613-616 PR:000012225 denotes PAF
T302 613-616 PR:Q9CQX4 denotes PAF
T301 613-616 CVCL_XY57 denotes PAF
T303 613-627 CHEBI:134182 denotes PAF antagonist
T305 628-636 C066686 denotes BN 50730
T306 628-636 C066686 denotes BN 50730
T307 644-649 P09958 denotes furin
T308 644-649 P23188 denotes furin
T309 644-649 PR:000003137 denotes furin
T311 644-649 PR:P09958 denotes furin
T312 644-649 Q28193 denotes furin
T313 644-649 PR:P23188 denotes furin
T314 644-649 P23377 denotes furin
T315 644-649 PR:P23377 denotes furin
T310 644-649 D045683 denotes furin
T316 650-659 CHEBI:35222 denotes inhibitor
T317 660-664 PR:Q04047 denotes nona
T318 665-670 CHEBI:32693 denotes D-arg
T319 665-670 CHEBI:32691 denotes D-arg
T320 665-670 CHEBI:29953 denotes D-arg
T322 667-670 PR:000013890 denotes arg
T324 667-670 PR:P42684 denotes arg
T325 667-670 PR:Q9P2R6 denotes arg
T327 667-670 PR:Q4JIM5 denotes arg
T331 667-670 PR:000003583 denotes arg
T321 667-670 SO:0001451 denotes arg
T332 667-675 C069339 denotes arg-NH(2
T333 667-675 C069339 denotes arg-NH(2
T341 678-693 GO:0010467 denotes Gene-expression
T342 705-710 P09958 denotes furin
T343 705-710 P23188 denotes furin
T344 705-710 PR:000003137 denotes furin
T346 705-710 PR:P09958 denotes furin
T347 705-710 Q28193 denotes furin
T348 705-710 PR:P23188 denotes furin
T349 705-710 P23377 denotes furin
T350 705-710 PR:P23377 denotes furin
T345 705-710 D045683 denotes furin
T351 712-721 D014568 denotes urokinase
T352 712-743 GO:0008243 denotes urokinase plasminogen activator
T371 722-743 P17811 denotes plasminogen activator
T372 722-743 D010960 denotes plasminogen activator
T373 745-748 CHEBI:340824 denotes MMP
T376 745-748 CHEBI:59761 denotes MMP
T377 745-750 PR:P08253 denotes MMP-2
T378 745-750 Q9GLE5 denotes MMP-2
T379 745-750 P08253 denotes MMP-2
T380 745-750 Q90611 denotes MMP-2
T382 745-750 PR:Q8MPP3 denotes MMP-2
T383 745-750 P33436 denotes MMP-2
T384 745-750 PR:Q90611 denotes MMP-2
T385 745-750 PR:P33436 denotes MMP-2
T386 745-750 PR:000010479 denotes MMP-2
T387 745-750 PR:P33434 denotes MMP-2
T388 745-750 P33434 denotes MMP-2
T389 745-750 P50757 denotes MMP-2
T381 745-750 GO:0004228 denotes MMP-2
T390 749-755 PR:O04529 denotes 2, MMP
T391 752-755 CHEBI:340824 denotes MMP
T394 752-755 CHEBI:59761 denotes MMP
T395 752-757 PR:P50282 denotes MMP-9
T396 752-757 PR:P14780 denotes MMP-9
T397 752-757 P14780 denotes MMP-9
T398 752-757 PR:P41245 denotes MMP-9
T399 752-757 P41246 denotes MMP-9
T400 752-757 P41245 denotes MMP-9
T401 752-757 PR:P52176 denotes MMP-9
T402 752-757 P50282 denotes MMP-9
T404 752-757 PR:000010491 denotes MMP-9
T405 752-757 P52176 denotes MMP-9
T406 752-757 O18733 denotes MMP-9
T403 752-757 GO:0004229 denotes MMP-9
T429 759-762 CVCL_2501 denotes MT1
T414 759-762 GO:0047152 denotes MT1
T423 759-762 GO:0043791 denotes MT1
T432 759-762 GO:0043834 denotes MT1
T448 759-766 Q9XT90 denotes MT1-MMP
T449 759-766 P50281 denotes MT1-MMP
T450 759-766 Q9GLE4 denotes MT1-MMP
T451 759-766 PR:000010474 denotes MT1-MMP
T452 759-766 Q10739 denotes MT1-MMP
T453 759-766 Q95220 denotes MT1-MMP
T455 759-766 P53690 denotes MT1-MMP
T454 759-766 D053511 denotes MT1-MMP
T458 763-766 GO:0004235 denotes MMP
T465 772-778 PR:P16035 denotes TIMP-2
T466 772-778 PR:000016353 denotes TIMP-2
T467 772-778 O42146 denotes TIMP-2
T468 772-778 Q9TRZ7 denotes TIMP-2
T469 772-778 Q9TTY1 denotes TIMP-2
T470 772-778 Q60453 denotes TIMP-2
T471 772-778 P16035 denotes TIMP-2
T473 772-778 P25785 denotes TIMP-2
T474 772-778 P16368 denotes TIMP-2
T475 772-778 O77717 denotes TIMP-2
T476 772-778 PR:P25785 denotes TIMP-2
T477 772-778 P30121 denotes TIMP-2
T478 772-778 Q9WUC6 denotes TIMP-2
T479 772-778 PR:P30121 denotes TIMP-2
T480 772-778 PR:O42146 denotes TIMP-2
T472 772-778 D019716 denotes TIMP-2
T481 808-811 Q41249 denotes PCR
T482 808-811 O66148 denotes PCR
T483 808-811 Q59987 denotes PCR
T484 808-811 Q01289 denotes PCR
T485 808-811 O80333 denotes PCR
T486 808-811 P15904 denotes PCR
T487 808-811 Q9SDT1 denotes PCR
T488 808-811 Q39617 denotes PCR
T489 813-820 CHEBI:16541 denotes Protein
T490 862-865 CHEBI:340824 denotes MMP
T493 862-865 CHEBI:59761 denotes MMP
T494 862-867 PR:P08253 denotes MMP-2
T495 862-867 Q9GLE5 denotes MMP-2
T496 862-867 P08253 denotes MMP-2
T497 862-867 Q90611 denotes MMP-2
T499 862-867 PR:Q8MPP3 denotes MMP-2
T500 862-867 P33436 denotes MMP-2
T501 862-867 PR:Q90611 denotes MMP-2
T502 862-867 PR:P33436 denotes MMP-2
T503 862-867 PR:000010479 denotes MMP-2
T504 862-867 PR:P33434 denotes MMP-2
T505 862-867 P33434 denotes MMP-2
T506 862-867 P50757 denotes MMP-2
T498 862-867 GO:0004228 denotes MMP-2
T507 902-909 4716 denotes gelatin
T508 902-909 D005780 denotes gelatin
T509 902-909 CHEBI:5291 denotes gelatin
T532 929-932 CVCL_2501 denotes MT1
T517 929-932 GO:0047152 denotes MT1
T526 929-932 GO:0043791 denotes MT1
T535 929-932 GO:0043834 denotes MT1
T551 929-936 Q9XT90 denotes MT1-MMP
T552 929-936 P50281 denotes MT1-MMP
T553 929-936 Q9GLE4 denotes MT1-MMP
T554 929-936 PR:000010474 denotes MT1-MMP
T555 929-936 Q10739 denotes MT1-MMP
T556 929-936 Q95220 denotes MT1-MMP
T558 929-936 P53690 denotes MT1-MMP
T557 929-936 D053511 denotes MT1-MMP
T561 933-936 GO:0004235 denotes MMP
T564 977-981 C083283 denotes cPAF
T565 977-981 C083283 denotes cPAF
T588 1016-1019 CVCL_2501 denotes MT1
T573 1016-1019 GO:0047152 denotes MT1
T582 1016-1019 GO:0043791 denotes MT1
T591 1016-1019 GO:0043834 denotes MT1
T607 1016-1023 Q9XT90 denotes MT1-MMP
T608 1016-1023 P50281 denotes MT1-MMP
T609 1016-1023 Q9GLE4 denotes MT1-MMP
T610 1016-1023 PR:000010474 denotes MT1-MMP
T611 1016-1023 Q10739 denotes MT1-MMP
T612 1016-1023 Q95220 denotes MT1-MMP
T614 1016-1023 P53690 denotes MT1-MMP
T613 1016-1023 D053511 denotes MT1-MMP
T617 1020-1023 GO:0004235 denotes MMP
T620 1025-1028 CHEBI:340824 denotes MMP
T623 1025-1028 CHEBI:59761 denotes MMP
T624 1025-1030 PR:P08253 denotes MMP-2
T625 1025-1030 Q9GLE5 denotes MMP-2
T626 1025-1030 P08253 denotes MMP-2
T627 1025-1030 Q90611 denotes MMP-2
T629 1025-1030 PR:Q8MPP3 denotes MMP-2
T630 1025-1030 P33436 denotes MMP-2
T631 1025-1030 PR:Q90611 denotes MMP-2
T632 1025-1030 PR:P33436 denotes MMP-2
T633 1025-1030 PR:000010479 denotes MMP-2
T634 1025-1030 PR:P33434 denotes MMP-2
T635 1025-1030 P33434 denotes MMP-2
T636 1025-1030 P50757 denotes MMP-2
T628 1025-1030 GO:0004228 denotes MMP-2
T637 1029-1035 PR:O04529 denotes 2, MMP
T638 1032-1035 CHEBI:340824 denotes MMP
T641 1032-1035 CHEBI:59761 denotes MMP
T642 1032-1037 PR:P50282 denotes MMP-9
T643 1032-1037 PR:P14780 denotes MMP-9
T644 1032-1037 P14780 denotes MMP-9
T645 1032-1037 PR:P41245 denotes MMP-9
T646 1032-1037 P41246 denotes MMP-9
T647 1032-1037 P41245 denotes MMP-9
T648 1032-1037 PR:P52176 denotes MMP-9
T649 1032-1037 P50282 denotes MMP-9
T651 1032-1037 PR:000010491 denotes MMP-9
T652 1032-1037 P52176 denotes MMP-9
T653 1032-1037 O18733 denotes MMP-9
T650 1032-1037 GO:0004229 denotes MMP-9
T658 1043-1049 PR:P16035 denotes TIMP-2
T659 1043-1049 PR:000016353 denotes TIMP-2
T660 1043-1049 O42146 denotes TIMP-2
T661 1043-1049 Q9TRZ7 denotes TIMP-2
T662 1043-1049 Q9TTY1 denotes TIMP-2
T663 1043-1049 Q60453 denotes TIMP-2
T664 1043-1049 P16035 denotes TIMP-2
T666 1043-1049 P25785 denotes TIMP-2
T667 1043-1049 P16368 denotes TIMP-2
T668 1043-1049 O77717 denotes TIMP-2
T669 1043-1049 PR:P25785 denotes TIMP-2
T670 1043-1049 P30121 denotes TIMP-2
T671 1043-1049 Q9WUC6 denotes TIMP-2
T672 1043-1049 PR:P30121 denotes TIMP-2
T673 1043-1049 PR:O42146 denotes TIMP-2
T665 1043-1049 D019716 denotes TIMP-2
T676 1050-1054 SO:0000234 denotes mRNA
T674 1050-1054 D012333 denotes mRNA
T675 1050-1054 CHEBI:33699 denotes mRNA
T699 1096-1099 CVCL_2501 denotes MT1
T684 1096-1099 GO:0047152 denotes MT1
T693 1096-1099 GO:0043791 denotes MT1
T702 1096-1099 GO:0043834 denotes MT1
T718 1096-1103 Q9XT90 denotes MT1-MMP
T719 1096-1103 P50281 denotes MT1-MMP
T720 1096-1103 Q9GLE4 denotes MT1-MMP
T721 1096-1103 PR:000010474 denotes MT1-MMP
T722 1096-1103 Q10739 denotes MT1-MMP
T723 1096-1103 Q95220 denotes MT1-MMP
T725 1096-1103 P53690 denotes MT1-MMP
T724 1096-1103 D053511 denotes MT1-MMP
T728 1100-1103 GO:0004235 denotes MMP
T731 1104-1111 GO:0003675 denotes protein
T735 1104-1111 SO:0000104 denotes protein
T734 1104-1111 PR:000000001 denotes protein
T732 1104-1111 CHEBI:36080 denotes protein
T733 1104-1111 CHEBI:11122 denotes protein
T740 1144-1150 PR:P16035 denotes TIMP-2
T741 1144-1150 PR:000016353 denotes TIMP-2
T742 1144-1150 O42146 denotes TIMP-2
T743 1144-1150 Q9TRZ7 denotes TIMP-2
T744 1144-1150 Q9TTY1 denotes TIMP-2
T745 1144-1150 Q60453 denotes TIMP-2
T746 1144-1150 P16035 denotes TIMP-2
T748 1144-1150 P25785 denotes TIMP-2
T749 1144-1150 P16368 denotes TIMP-2
T750 1144-1150 O77717 denotes TIMP-2
T751 1144-1150 PR:P25785 denotes TIMP-2
T752 1144-1150 P30121 denotes TIMP-2
T753 1144-1150 Q9WUC6 denotes TIMP-2
T754 1144-1150 PR:P30121 denotes TIMP-2
T755 1144-1150 PR:O42146 denotes TIMP-2
T747 1144-1150 D019716 denotes TIMP-2
T756 1151-1158 GO:0003675 denotes protein
T760 1151-1158 SO:0000104 denotes protein
T759 1151-1158 PR:000000001 denotes protein
T757 1151-1158 CHEBI:36080 denotes protein
T758 1151-1158 CHEBI:11122 denotes protein
T762 1182-1185 PR:Q6RIA2 denotes PAF
T763 1182-1185 PR:Q15004 denotes PAF
T766 1182-1185 PR:000012225 denotes PAF
T768 1182-1185 PR:Q9CQX4 denotes PAF
T767 1182-1185 CVCL_XY57 denotes PAF
T764 1182-1185 CHEBI:52450 denotes PAF
T765 1182-1185 CHEBI:44811 denotes PAF
T769 1199-1204 P09958 denotes furin
T770 1199-1204 P23188 denotes furin
T771 1199-1204 PR:000003137 denotes furin
T773 1199-1204 PR:P09958 denotes furin
T774 1199-1204 Q28193 denotes furin
T775 1199-1204 PR:P23188 denotes furin
T776 1199-1204 P23377 denotes furin
T777 1199-1204 PR:P23377 denotes furin
T772 1199-1204 D045683 denotes furin
T778 1205-1209 SO:0000704 denotes gene
T779 1205-1220 GO:0010467 denotes gene expression
T780 1244-1251 GO:0003675 denotes protein
T784 1244-1251 SO:0000104 denotes protein
T783 1244-1251 PR:000000001 denotes protein
T781 1244-1251 CHEBI:36080 denotes protein
T782 1244-1251 CHEBI:11122 denotes protein
T785 1264-1268 PR:Q04047 denotes Nona
T786 1269-1274 CHEBI:32693 denotes D-arg
T787 1269-1274 CHEBI:32691 denotes D-arg
T788 1269-1274 CHEBI:29953 denotes D-arg
T790 1271-1274 PR:000013890 denotes arg
T792 1271-1274 PR:P42684 denotes arg
T793 1271-1274 PR:Q9P2R6 denotes arg
T795 1271-1274 PR:Q4JIM5 denotes arg
T799 1271-1274 PR:000003583 denotes arg
T789 1271-1274 SO:0001451 denotes arg
T800 1271-1279 C069339 denotes arg-NH(2
T801 1271-1279 C069339 denotes arg-NH(2
T809 1289-1293 C083283 denotes cPAF
T810 1289-1293 C083283 denotes cPAF
T818 1307-1310 GO:0047152 denotes MT1
T827 1307-1310 GO:0043791 denotes MT1
T836 1307-1310 GO:0043834 denotes MT1
T833 1307-1310 CVCL_2501 denotes MT1
T852 1307-1314 Q9XT90 denotes MT1-MMP
T853 1307-1314 P50281 denotes MT1-MMP
T854 1307-1314 Q9GLE4 denotes MT1-MMP
T855 1307-1314 PR:000010474 denotes MT1-MMP
T856 1307-1314 Q10739 denotes MT1-MMP
T857 1307-1314 Q95220 denotes MT1-MMP
T859 1307-1314 P53690 denotes MT1-MMP
T858 1307-1314 D053511 denotes MT1-MMP
T862 1311-1314 GO:0004235 denotes MMP
T867 1325-1328 CHEBI:52450 denotes PAF
T868 1325-1328 CHEBI:44811 denotes PAF
T865 1325-1328 PR:Q6RIA2 denotes PAF
T866 1325-1328 PR:Q15004 denotes PAF
T869 1325-1328 PR:000012225 denotes PAF
T871 1325-1328 PR:Q9CQX4 denotes PAF
T870 1325-1328 CVCL_XY57 denotes PAF
T872 1376-1379 CHEBI:340824 denotes MMP
T875 1376-1379 CHEBI:59761 denotes MMP
T876 1376-1381 PR:P50282 denotes MMP-9
T877 1376-1381 PR:P14780 denotes MMP-9
T878 1376-1381 P14780 denotes MMP-9
T879 1376-1381 PR:P41245 denotes MMP-9
T880 1376-1381 P41246 denotes MMP-9
T881 1376-1381 P41245 denotes MMP-9
T882 1376-1381 PR:P52176 denotes MMP-9
T883 1376-1381 P50282 denotes MMP-9
T885 1376-1381 PR:000010491 denotes MMP-9
T886 1376-1381 P52176 denotes MMP-9
T887 1376-1381 O18733 denotes MMP-9
T884 1376-1381 GO:0004229 denotes MMP-9
T892 1382-1389 SO:0000104 denotes protein
T889 1382-1389 CHEBI:36080 denotes protein
T890 1382-1389 CHEBI:11122 denotes protein
T891 1382-1389 PR:000000001 denotes protein
T888 1382-1389 GO:0003675 denotes protein
T893 1405-1408 CHEBI:340824 denotes MMP
T896 1405-1408 CHEBI:59761 denotes MMP
T897 1405-1410 PR:P08253 denotes MMP-2
T898 1405-1410 Q9GLE5 denotes MMP-2
T899 1405-1410 P08253 denotes MMP-2
T900 1405-1410 Q90611 denotes MMP-2
T902 1405-1410 PR:Q8MPP3 denotes MMP-2
T903 1405-1410 P33436 denotes MMP-2
T904 1405-1410 PR:Q90611 denotes MMP-2
T905 1405-1410 PR:P33436 denotes MMP-2
T906 1405-1410 PR:000010479 denotes MMP-2
T907 1405-1410 PR:P33434 denotes MMP-2
T908 1405-1410 P33434 denotes MMP-2
T909 1405-1410 P50757 denotes MMP-2
T901 1405-1410 GO:0004228 denotes MMP-2
T910 1439-1447 C066686 denotes BN 50730
T911 1439-1447 C066686 denotes BN 50730
T912 1456-1459 PR:Q6RIA2 denotes PAF
T913 1456-1459 PR:Q15004 denotes PAF
T916 1456-1459 PR:000012225 denotes PAF
T918 1456-1459 PR:Q9CQX4 denotes PAF
T917 1456-1459 CVCL_XY57 denotes PAF
T914 1456-1459 CHEBI:52450 denotes PAF
T915 1456-1459 CHEBI:44811 denotes PAF
T919 1468-1481 GO:0006351 denotes transcription
T920 1486-1497 GO:0006412 denotes translation
T921 1507-1515 CHEBI:36080 denotes proteins
T922 1530-1533 PR:Q6RIA2 denotes PAF
T923 1530-1533 PR:Q15004 denotes PAF
T926 1530-1533 PR:000012225 denotes PAF
T928 1530-1533 PR:Q9CQX4 denotes PAF
T927 1530-1533 CVCL_XY57 denotes PAF
T924 1530-1533 CHEBI:52450 denotes PAF
T925 1530-1533 CHEBI:44811 denotes PAF
T929 1604-1609 P09958 denotes furin
T930 1604-1609 P23188 denotes furin
T931 1604-1609 PR:000003137 denotes furin
T933 1604-1609 PR:P09958 denotes furin
T934 1604-1609 Q28193 denotes furin
T935 1604-1609 PR:P23188 denotes furin
T936 1604-1609 P23377 denotes furin
T937 1604-1609 PR:P23377 denotes furin
T932 1604-1609 D045683 denotes furin
T940 1611-1614 PR:O04529 denotes MMP
T938 1611-1614 CHEBI:340824 denotes MMP
T941 1611-1614 CHEBI:59761 denotes MMP
T939 1611-1614 GO:0004235 denotes MMP
T946 1620-1626 PR:P16035 denotes TIMP-2
T947 1620-1626 PR:000016353 denotes TIMP-2
T948 1620-1626 O42146 denotes TIMP-2
T949 1620-1626 Q9TRZ7 denotes TIMP-2
T950 1620-1626 Q9TTY1 denotes TIMP-2
T951 1620-1626 Q60453 denotes TIMP-2
T952 1620-1626 P16035 denotes TIMP-2
T954 1620-1626 P25785 denotes TIMP-2
T955 1620-1626 P16368 denotes TIMP-2
T956 1620-1626 O77717 denotes TIMP-2
T957 1620-1626 PR:P25785 denotes TIMP-2
T958 1620-1626 P30121 denotes TIMP-2
T959 1620-1626 Q9WUC6 denotes TIMP-2
T960 1620-1626 PR:P30121 denotes TIMP-2
T961 1620-1626 PR:O42146 denotes TIMP-2
T953 1620-1626 D019716 denotes TIMP-2
T962 1665-1668 CHEBI:340824 denotes MMP
T965 1665-1668 CHEBI:59761 denotes MMP
T966 1665-1670 PR:P08253 denotes MMP-2
T967 1665-1670 Q9GLE5 denotes MMP-2
T968 1665-1670 P08253 denotes MMP-2
T969 1665-1670 Q90611 denotes MMP-2
T971 1665-1670 PR:Q8MPP3 denotes MMP-2
T972 1665-1670 P33436 denotes MMP-2
T973 1665-1670 PR:Q90611 denotes MMP-2
T974 1665-1670 PR:P33436 denotes MMP-2
T975 1665-1670 PR:000010479 denotes MMP-2
T976 1665-1670 PR:P33434 denotes MMP-2
T977 1665-1670 P33434 denotes MMP-2
T978 1665-1670 P50757 denotes MMP-2
T970 1665-1670 GO:0004228 denotes MMP-2
T979 1697-1700 PR:Q6RIA2 denotes PAF
T980 1697-1700 PR:Q15004 denotes PAF
T983 1697-1700 PR:000012225 denotes PAF
T985 1697-1700 PR:Q9CQX4 denotes PAF
T984 1697-1700 CVCL_XY57 denotes PAF
T981 1697-1700 CHEBI:52450 denotes PAF
T982 1697-1700 CHEBI:44811 denotes PAF
T986 1780-1783 PR:Q6RIA2 denotes PAF
T987 1780-1783 PR:Q15004 denotes PAF
T990 1780-1783 PR:000012225 denotes PAF
T992 1780-1783 PR:Q9CQX4 denotes PAF
T991 1780-1783 CVCL_XY57 denotes PAF
T988 1780-1783 CHEBI:52450 denotes PAF
T989 1780-1783 CHEBI:44811 denotes PAF
T1015 1798-1801 CVCL_2501 denotes MT1
T1000 1798-1801 GO:0047152 denotes MT1
T1009 1798-1801 GO:0043791 denotes MT1
T1018 1798-1801 GO:0043834 denotes MT1
T1034 1798-1805 Q9XT90 denotes MT1-MMP
T1035 1798-1805 P50281 denotes MT1-MMP
T1036 1798-1805 Q9GLE4 denotes MT1-MMP
T1037 1798-1805 PR:000010474 denotes MT1-MMP
T1038 1798-1805 Q10739 denotes MT1-MMP
T1039 1798-1805 Q95220 denotes MT1-MMP
T1041 1798-1805 P53690 denotes MT1-MMP
T1040 1798-1805 D053511 denotes MT1-MMP
T1044 1802-1805 GO:0004235 denotes MMP
T1047 1824-1827 CHEBI:340824 denotes MMP
T1050 1824-1827 CHEBI:59761 denotes MMP
T1051 1824-1829 PR:P08253 denotes MMP-2
T1052 1824-1829 Q9GLE5 denotes MMP-2
T1053 1824-1829 P08253 denotes MMP-2
T1054 1824-1829 Q90611 denotes MMP-2
T1056 1824-1829 PR:Q8MPP3 denotes MMP-2
T1057 1824-1829 P33436 denotes MMP-2
T1058 1824-1829 PR:Q90611 denotes MMP-2
T1059 1824-1829 PR:P33436 denotes MMP-2
T1060 1824-1829 PR:000010479 denotes MMP-2
T1061 1824-1829 PR:P33434 denotes MMP-2
T1062 1824-1829 P33434 denotes MMP-2
T1063 1824-1829 P50757 denotes MMP-2
T1055 1824-1829 GO:0004228 denotes MMP-2
T1066 1872-1875 CHEBI:52450 denotes PAF
T1067 1872-1875 CHEBI:44811 denotes PAF
T1064 1872-1875 PR:Q6RIA2 denotes PAF
T1065 1872-1875 PR:Q15004 denotes PAF
T1068 1872-1875 PR:000012225 denotes PAF
T1070 1872-1875 PR:Q9CQX4 denotes PAF
T1069 1872-1875 CVCL_XY57 denotes PAF
T1072 1896-1916 GO:0031012 denotes extracellular matrix
T1073 1910-1916 UBERON:0027368 denotes matrix
T1074 2005-2013 D002921 denotes scarring
T1075 2005-2013 D002921 denotes scarring
T1076 2033-2036 PR:Q6RIA2 denotes PAF
T1077 2033-2036 PR:Q15004 denotes PAF
T1080 2033-2036 PR:000012225 denotes PAF
T1082 2033-2036 PR:Q9CQX4 denotes PAF
T1081 2033-2036 CVCL_XY57 denotes PAF
T1083 2033-2048 CHEBI:134182 denotes PAF antagonists

UseCases_ArguminSci_Discourse

Id Subject Object Predicate Lexical cue
T1 0-102 DRI_Approach denotes PAF-induced furin and MT1-MMP expression is independent of MMP-2 activation in corneal myofibroblasts.
T2 112-222 DRI_Outcome denotes Corneal stromal myofibroblasts express the platelet-activating factor (PAF) receptor, but its role is unclear.
T3 223-322 DRI_Background denotes In the present study, the effect of PAF on induction of metalloproteinases (MMPs) was investigated.
T4 332-437 DRI_Background denotes Rabbit corneal myofibroblasts were identified by immunodetection of alpha-smooth muscle (alpha-SM)-actin.
T5 438-555 DRI_Background denotes MT1-MMP, MMP-2, MMP-9, and tissue inhibitor of matrix metalloproteinase (TIMP)-2 were detected by immunofluorescence.
T6 556-677 DRI_Approach denotes Cells were treated with 100 nM cPAF, with or without the PAF antagonist BN 50730 or the furin inhibitor nona-D-arg-NH(2).
T7 678-812 DRI_Background denotes Gene-expression levels for furin, urokinase plasminogen activator, MMP-2, MMP-9, MT1-MMP, and TIMP-2 were determined by real-time PCR.
T8 813-861 DRI_Background denotes Protein expression was assessed by Western blot.
T9 862-921 DRI_Unspecified denotes MMP-2 and -9 activity was determined by gelatin zymography.
T10 922-967 DRI_Background denotes Active MT1-MMP levels were measured by ELISA.
T11 977-1181 DRI_Background denotes cPAF triggered significantly increased MT1-MMP, MMP-2, MMP-9, and TIMP-2 mRNA expression, followed by increased active MT1-MMP protein expression at 12 hours, whereas TIMP-2 protein increased at 24 hours.
T12 1182-1263 DRI_Background denotes PAF also induced furin gene expression, followed by increased protein expression.
T13 1264-1324 DRI_Unspecified denotes Nona-D-arg-NH(2) blocked cPAF induction of MT1-MMP activity.
T14 1325-1336 REPLACED denotes PAF-treated
T15 1337-1351 Token_Label.OUTSIDE denotes myofibroblasts
T16 1352-1420 DRI_Unspecified denotes showed increased active MMP-9 protein, but unchanged MMP-2 activity.
T17 1421-1516 DRI_Background denotes Pretreatment with BN 50730 blocked PAF-induced transcription and translation of these proteins.
T18 1530-1664 DRI_Approach denotes PAF, through a receptor-mediated mechanism, induces a specific pattern of furin, MMP, and TIMP-2 expression in corneal myofibroblasts.
T19 1665-1711 DRI_Unspecified denotes MMP-2 activity was unchanged by PAF treatment.
T20 1712-1865 DRI_Challenge denotes These results suggest that in response to the inflammatory mediator PAF, induction of MT1-MMP is independent of MMP-2 activity in corneal myofibroblasts.
T21 1866-2022 DRI_Approach denotes Thus, PAF-mediated changes in extracellular matrix composition surrounding the myofibroblasts could be important in regulating the corneal scarring process.
T22 2023-2101 DRI_Outcome denotes Moreover, PAF antagonists could be useful in maintaining corneal transparency.