PubMed:1533441
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/1533441","sourcedb":"PubMed","sourceid":"1533441","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/1533441","text":"Nuclear factor of activated T cells contains Fos and Jun.\nThe nuclear factor NF-AT (ref. 1) is induced in T cells stimulated through the T-cell receptor/CD3 complex, and is required for interleukin-2 (IL-2) gene induction. Although NF-AT has not been cloned or purified, there is evidence that it is a major target for immunosuppression by cyclosporin A (CsA) and FK506 (refs 2-7). NF-AT induction may require two activation-dependent events: the CsA-sensitive translocation of a pre-existing component and the CsA-resistant synthesis of a nuclear component. Here we report that the newly synthesized nuclear component of NF-AT is the transcription factor AP-1. We show that the inducible nuclear form of NF-AT contains Fos and Jun proteins. Furthermore, we identify a pre-existing NF-AT-binding factor that is present in hypotonic extracts of unstimulated T cells. On the basis of binding, reconstitution and cotransfection experiments, we propose that activation of NF-AT occurs in at least two stages: a CsA-sensitive stage involving modification and/or translocation of the pre-existing NF-AT complex, and a CsA-insensitive stage involving the addition of newly synthesized Fos or Fos/Jun proteins to the pre-existing complex.","tracks":[{"project":"genia-medco-coref","denotations":[{"id":"C2","span":{"begin":18,"end":35},"obj":"NP"},{"id":"C1","span":{"begin":0,"end":35},"obj":"NP"},{"id":"C3","span":{"begin":45,"end":56},"obj":"NP"},{"id":"C4","span":{"begin":58,"end":82},"obj":"NP"},{"id":"C5","span":{"begin":106,"end":164},"obj":"NP"},{"id":"C6","span":{"begin":232,"end":237},"obj":"NP"},{"id":"C7","span":{"begin":294,"end":296},"obj":"NP"},{"id":"C8","span":{"begin":340,"end":359},"obj":"NP"},{"id":"C9","span":{"begin":382,"end":387},"obj":"NP"},{"id":"C10","span":{"begin":447,"end":450},"obj":"NP"},{"id":"C11","span":{"begin":511,"end":514},"obj":"NP"},{"id":"C12","span":{"begin":538,"end":557},"obj":"NP"},{"id":"C14","span":{"begin":622,"end":627},"obj":"NP"},{"id":"C13","span":{"begin":579,"end":627},"obj":"NP"},{"id":"C15","span":{"begin":631,"end":660},"obj":"NP"},{"id":"C16","span":{"begin":705,"end":710},"obj":"NP"},{"id":"C17","span":{"begin":720,"end":740},"obj":"NP"},{"id":"C18","span":{"begin":767,"end":802},"obj":"NP"},{"id":"C19","span":{"begin":803,"end":807},"obj":"NP"},{"id":"C20","span":{"begin":968,"end":973},"obj":"NP"},{"id":"C21","span":{"begin":1007,"end":1010},"obj":"NP"},{"id":"C22","span":{"begin":1074,"end":1104},"obj":"NP"},{"id":"C23","span":{"begin":1112,"end":1115},"obj":"NP"},{"id":"C24","span":{"begin":1205,"end":1229},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-other","subj":"C4","obj":"C1"},{"id":"R2","pred":"coref-other","subj":"C5","obj":"C2"},{"id":"R3","pred":"coref-ident","subj":"C6","obj":"C4"},{"id":"R4","pred":"coref-pron","subj":"C7","obj":"C6"},{"id":"R5","pred":"coref-ident","subj":"C9","obj":"C6"},{"id":"R6","pred":"coref-ident","subj":"C10","obj":"C8"},{"id":"R7","pred":"coref-ident","subj":"C11","obj":"C10"},{"id":"R8","pred":"coref-ident","subj":"C14","obj":"C9"},{"id":"R9","pred":"coref-other","subj":"C13","obj":"C12"},{"id":"R10","pred":"coref-ident","subj":"C15","obj":"C13"},{"id":"R11","pred":"coref-ident","subj":"C16","obj":"C14"},{"id":"R12","pred":"coref-ident","subj":"C17","obj":"C3"},{"id":"R13","pred":"coref-relat","subj":"C19","obj":"C18"},{"id":"R14","pred":"coref-ident","subj":"C20","obj":"C16"},{"id":"R15","pred":"coref-ident","subj":"C21","obj":"C11"},{"id":"R16","pred":"coref-ident","subj":"C23","obj":"C21"},{"id":"R17","pred":"coref-ident","subj":"C24","obj":"C22"}],"attributes":[{"subj":"C2","pred":"source","obj":"genia-medco-coref"},{"subj":"C1","pred":"source","obj":"genia-medco-coref"},{"subj":"C3","pred":"source","obj":"genia-medco-coref"},{"subj":"C4","pred":"source","obj":"genia-medco-coref"},{"subj":"C5","pred":"source","obj":"genia-medco-coref"},{"subj":"C6","pred":"source","obj":"genia-medco-coref"},{"subj":"C7","pred":"source","obj":"genia-medco-coref"},{"subj":"C8","pred":"source","obj":"genia-medco-coref"},{"subj":"C9","pred":"source","obj":"genia-medco-coref"},{"subj":"C10","pred":"source","obj":"genia-medco-coref"},{"subj":"C11","pred":"source","obj":"genia-medco-coref"},{"subj":"C12","pred":"source","obj":"genia-medco-coref"},{"subj":"C14","pred":"source","obj":"genia-medco-coref"},{"subj":"C13","pred":"source","obj":"genia-medco-coref"},{"subj":"C15","pred":"source","obj":"genia-medco-coref"},{"subj":"C16","pred":"source","obj":"genia-medco-coref"},{"subj":"C17","pred":"source","obj":"genia-medco-coref"},{"subj":"C18","pred":"source","obj":"genia-medco-coref"},{"subj":"C19","pred":"source","obj":"genia-medco-coref"},{"subj":"C20","pred":"source","obj":"genia-medco-coref"},{"subj":"C21","pred":"source","obj":"genia-medco-coref"},{"subj":"C22","pred":"source","obj":"genia-medco-coref"},{"subj":"C23","pred":"source","obj":"genia-medco-coref"},{"subj":"C24","pred":"source","obj":"genia-medco-coref"}]},{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":0,"end":14},"obj":"protein"},{"id":"T2","span":{"begin":18,"end":35},"obj":"cell_type"},{"id":"T3","span":{"begin":45,"end":48},"obj":"protein"},{"id":"T4","span":{"begin":53,"end":56},"obj":"protein"},{"id":"T5","span":{"begin":62,"end":82},"obj":"protein"},{"id":"T6","span":{"begin":106,"end":113},"obj":"cell_type"},{"id":"T7","span":{"begin":137,"end":164},"obj":"protein"},{"id":"T8","span":{"begin":186,"end":211},"obj":"DNA"},{"id":"T9","span":{"begin":232,"end":237},"obj":"protein"},{"id":"T10","span":{"begin":382,"end":387},"obj":"protein"},{"id":"T11","span":{"begin":540,"end":557},"obj":"protein"},{"id":"T12","span":{"begin":601,"end":618},"obj":"protein"},{"id":"T13","span":{"begin":622,"end":627},"obj":"protein"},{"id":"T14","span":{"begin":635,"end":655},"obj":"protein"},{"id":"T15","span":{"begin":656,"end":660},"obj":"protein"},{"id":"T16","span":{"begin":705,"end":710},"obj":"protein"},{"id":"T17","span":{"begin":720,"end":723},"obj":"protein"},{"id":"T18","span":{"begin":728,"end":731},"obj":"protein"},{"id":"T19","span":{"begin":769,"end":802},"obj":"protein"},{"id":"T20","span":{"begin":844,"end":864},"obj":"cell_type"},{"id":"T21","span":{"begin":968,"end":973},"obj":"protein"},{"id":"T22","span":{"begin":1091,"end":1104},"obj":"protein"},{"id":"T23","span":{"begin":1178,"end":1181},"obj":"protein"},{"id":"T24","span":{"begin":1185,"end":1201},"obj":"protein"},{"id":"T25","span":{"begin":1209,"end":1229},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"jnlpba-st-training"},{"subj":"T2","pred":"source","obj":"jnlpba-st-training"},{"subj":"T3","pred":"source","obj":"jnlpba-st-training"},{"subj":"T4","pred":"source","obj":"jnlpba-st-training"},{"subj":"T5","pred":"source","obj":"jnlpba-st-training"},{"subj":"T6","pred":"source","obj":"jnlpba-st-training"},{"subj":"T7","pred":"source","obj":"jnlpba-st-training"},{"subj":"T8","pred":"source","obj":"jnlpba-st-training"},{"subj":"T9","pred":"source","obj":"jnlpba-st-training"},{"subj":"T10","pred":"source","obj":"jnlpba-st-training"},{"subj":"T11","pred":"source","obj":"jnlpba-st-training"},{"subj":"T12","pred":"source","obj":"jnlpba-st-training"},{"subj":"T13","pred":"source","obj":"jnlpba-st-training"},{"subj":"T14","pred":"source","obj":"jnlpba-st-training"},{"subj":"T15","pred":"source","obj":"jnlpba-st-training"},{"subj":"T16","pred":"source","obj":"jnlpba-st-training"},{"subj":"T17","pred":"source","obj":"jnlpba-st-training"},{"subj":"T18","pred":"source","obj":"jnlpba-st-training"},{"subj":"T19","pred":"source","obj":"jnlpba-st-training"},{"subj":"T20","pred":"source","obj":"jnlpba-st-training"},{"subj":"T21","pred":"source","obj":"jnlpba-st-training"},{"subj":"T22","pred":"source","obj":"jnlpba-st-training"},{"subj":"T23","pred":"source","obj":"jnlpba-st-training"},{"subj":"T24","pred":"source","obj":"jnlpba-st-training"},{"subj":"T25","pred":"source","obj":"jnlpba-st-training"}]},{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":57},"obj":"Sentence"},{"id":"S2","span":{"begin":58,"end":222},"obj":"Sentence"},{"id":"S3","span":{"begin":223,"end":381},"obj":"Sentence"},{"id":"S4","span":{"begin":382,"end":558},"obj":"Sentence"},{"id":"S5","span":{"begin":559,"end":661},"obj":"Sentence"},{"id":"S6","span":{"begin":662,"end":741},"obj":"Sentence"},{"id":"S7","span":{"begin":742,"end":865},"obj":"Sentence"},{"id":"S8","span":{"begin":866,"end":1230},"obj":"Sentence"}],"attributes":[{"subj":"S1","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S2","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S3","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S4","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S5","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S6","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S7","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S8","pred":"source","obj":"pubmed-sentences-benchmark"}]},{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":0,"end":14},"obj":"protein_family_or_group"},{"id":"T2","span":{"begin":18,"end":35},"obj":"cell_type"},{"id":"T3","span":{"begin":45,"end":48},"obj":"protein_molecule"},{"id":"T4","span":{"begin":53,"end":56},"obj":"protein_family_or_group"},{"id":"T5","span":{"begin":62,"end":82},"obj":"protein_molecule"},{"id":"T6","span":{"begin":106,"end":113},"obj":"cell_type"},{"id":"T7","span":{"begin":137,"end":164},"obj":"protein_complex"},{"id":"T8","span":{"begin":186,"end":199},"obj":"protein_molecule"},{"id":"T9","span":{"begin":201,"end":205},"obj":"protein_molecule"},{"id":"T10","span":{"begin":232,"end":237},"obj":"protein_molecule"},{"id":"T11","span":{"begin":340,"end":353},"obj":"other_organic_compound"},{"id":"T12","span":{"begin":355,"end":358},"obj":"other_organic_compound"},{"id":"T13","span":{"begin":364,"end":369},"obj":"other_organic_compound"},{"id":"T14","span":{"begin":382,"end":387},"obj":"protein_molecule"},{"id":"T15","span":{"begin":447,"end":450},"obj":"other_organic_compound"},{"id":"T16","span":{"begin":511,"end":514},"obj":"other_organic_compound"},{"id":"T17","span":{"begin":540,"end":557},"obj":"protein_family_or_group"},{"id":"T18","span":{"begin":601,"end":618},"obj":"protein_molecule"},{"id":"T19","span":{"begin":622,"end":627},"obj":"protein_molecule"},{"id":"T20","span":{"begin":635,"end":655},"obj":"protein_family_or_group"},{"id":"T21","span":{"begin":656,"end":660},"obj":"protein_molecule"},{"id":"T22","span":{"begin":705,"end":710},"obj":"protein_molecule"},{"id":"T23","span":{"begin":720,"end":723},"obj":"protein_molecule"},{"id":"T24","span":{"begin":728,"end":731},"obj":"protein_family_or_group"},{"id":"T25","span":{"begin":769,"end":781},"obj":"protein_molecule"},{"id":"T26","span":{"begin":782,"end":787},"obj":"protein_molecule"},{"id":"T27","span":{"begin":844,"end":856},"obj":"cell_type"},{"id":"T28","span":{"begin":857,"end":864},"obj":"cell_type"},{"id":"T29","span":{"begin":968,"end":973},"obj":"protein_molecule"},{"id":"T30","span":{"begin":1007,"end":1010},"obj":"other_organic_compound"},{"id":"T31","span":{"begin":1091,"end":1096},"obj":"protein_molecule"},{"id":"T32","span":{"begin":1112,"end":1115},"obj":"other_organic_compound"},{"id":"T33","span":{"begin":1178,"end":1181},"obj":"protein_molecule"},{"id":"T34","span":{"begin":1185,"end":1201},"obj":"protein_molecule"},{"id":"T35","span":{"begin":1209,"end":1229},"obj":"protein_complex"}],"attributes":[{"subj":"T1","pred":"source","obj":"GENIAcorpus"},{"subj":"T2","pred":"source","obj":"GENIAcorpus"},{"subj":"T3","pred":"source","obj":"GENIAcorpus"},{"subj":"T4","pred":"source","obj":"GENIAcorpus"},{"subj":"T5","pred":"source","obj":"GENIAcorpus"},{"subj":"T6","pred":"source","obj":"GENIAcorpus"},{"subj":"T7","pred":"source","obj":"GENIAcorpus"},{"subj":"T8","pred":"source","obj":"GENIAcorpus"},{"subj":"T9","pred":"source","obj":"GENIAcorpus"},{"subj":"T10","pred":"source","obj":"GENIAcorpus"},{"subj":"T11","pred":"source","obj":"GENIAcorpus"},{"subj":"T12","pred":"source","obj":"GENIAcorpus"},{"subj":"T13","pred":"source","obj":"GENIAcorpus"},{"subj":"T14","pred":"source","obj":"GENIAcorpus"},{"subj":"T15","pred":"source","obj":"GENIAcorpus"},{"subj":"T16","pred":"source","obj":"GENIAcorpus"},{"subj":"T17","pred":"source","obj":"GENIAcorpus"},{"subj":"T18","pred":"source","obj":"GENIAcorpus"},{"subj":"T19","pred":"source","obj":"GENIAcorpus"},{"subj":"T20","pred":"source","obj":"GENIAcorpus"},{"subj":"T21","pred":"source","obj":"GENIAcorpus"},{"subj":"T22","pred":"source","obj":"GENIAcorpus"},{"subj":"T23","pred":"source","obj":"GENIAcorpus"},{"subj":"T24","pred":"source","obj":"GENIAcorpus"},{"subj":"T25","pred":"source","obj":"GENIAcorpus"},{"subj":"T26","pred":"source","obj":"GENIAcorpus"},{"subj":"T27","pred":"source","obj":"GENIAcorpus"},{"subj":"T28","pred":"source","obj":"GENIAcorpus"},{"subj":"T29","pred":"source","obj":"GENIAcorpus"},{"subj":"T30","pred":"source","obj":"GENIAcorpus"},{"subj":"T31","pred":"source","obj":"GENIAcorpus"},{"subj":"T32","pred":"source","obj":"GENIAcorpus"},{"subj":"T33","pred":"source","obj":"GENIAcorpus"},{"subj":"T34","pred":"source","obj":"GENIAcorpus"},{"subj":"T35","pred":"source","obj":"GENIAcorpus"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"genia-medco-coref","color":"#d893ec","default":true},{"id":"jnlpba-st-training","color":"#93ecbe"},{"id":"pubmed-sentences-benchmark","color":"#eca493"},{"id":"GENIAcorpus","color":"#939cec"}]}]}}