PubMed:15044014
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/15044014","sourcedb":"PubMed","sourceid":"15044014","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/15044014","text":"Molecular cloning and characterization of beta1,4-N-acetylgalactosaminyltransferases IV synthesizing N,N'-diacetyllactosediamine.\nA sequence highly homologous to beta1,4-N-acetylgalactosaminyltransferase III (beta4GalNAc-T3) was found in a database of human expressed sequence tags. The full-length open reading frame of the gene, beta4GalNAc-T4 (GenBank accession number AB089939), was cloned using the 5' rapid amplification of cDNA ends method. It encodes a typical type II transmembrane protein of 1039 amino acids having 42.6% identity with beta4GalNAc-T3. The recombinant enzyme transferred N-acetylgalactosamine to N-acetylglucosamine-beta-benzyl with a beta1,4-linkage to form N,N'-diacetyllactosediamine as did beta4GalNAc-T3. In specificity toward oligosaccharide acceptor substrates, it was quite similar to beta4GalNAc-T3 in vitro, however, the tissue distributions of the two enzymes were quite different. These results indicated that the two enzymes have similar roles in different tissues.","tracks":[{"project":"glycogenes","denotations":[{"id":"PD-GlycoGenes20190927-B_T1","span":{"begin":42,"end":84},"obj":"https://acgg.asia/db/ggdb/info/gg107"},{"id":"PD-GlycoGenes20190927-B_T2","span":{"begin":42,"end":84},"obj":"https://acgg.asia/db/ggdb/info/gg106"},{"id":"PD-GlycoGenes20190927-B_T3","span":{"begin":42,"end":84},"obj":"https://acgg.asia/db/ggdb/info/gg105"},{"id":"PD-GlycoGenes20190927-B_T4","span":{"begin":162,"end":203},"obj":"https://acgg.asia/db/ggdb/info/gg107"},{"id":"PD-GlycoGenes20190927-B_T5","span":{"begin":162,"end":203},"obj":"https://acgg.asia/db/ggdb/info/gg106"},{"id":"PD-GlycoGenes20190927-B_T6","span":{"begin":162,"end":203},"obj":"https://acgg.asia/db/ggdb/info/gg105"},{"id":"PD-GlycoGenes20190927-B_T7","span":{"begin":474,"end":476},"obj":"https://acgg.asia/db/ggdb/info/gg111"}],"attributes":[{"subj":"PD-GlycoGenes20190927-B_T1","pred":"source","obj":"glycogenes"},{"subj":"PD-GlycoGenes20190927-B_T2","pred":"source","obj":"glycogenes"},{"subj":"PD-GlycoGenes20190927-B_T3","pred":"source","obj":"glycogenes"},{"subj":"PD-GlycoGenes20190927-B_T4","pred":"source","obj":"glycogenes"},{"subj":"PD-GlycoGenes20190927-B_T5","pred":"source","obj":"glycogenes"},{"subj":"PD-GlycoGenes20190927-B_T6","pred":"source","obj":"glycogenes"},{"subj":"PD-GlycoGenes20190927-B_T7","pred":"source","obj":"glycogenes"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"glycogenes","color":"#ec93e4","default":true}]}]}}