PubMed:15044014 JSONTXT

{"project":"glycogenes","denotations":[{"id":"PD-GlycoGenes20190927-B_T1","span":{"begin":42,"end":84},"obj":"https://acgg.asia/db/ggdb/info/gg107"},{"id":"PD-GlycoGenes20190927-B_T2","span":{"begin":42,"end":84},"obj":"https://acgg.asia/db/ggdb/info/gg106"},{"id":"PD-GlycoGenes20190927-B_T3","span":{"begin":42,"end":84},"obj":"https://acgg.asia/db/ggdb/info/gg105"},{"id":"PD-GlycoGenes20190927-B_T4","span":{"begin":162,"end":203},"obj":"https://acgg.asia/db/ggdb/info/gg107"},{"id":"PD-GlycoGenes20190927-B_T5","span":{"begin":162,"end":203},"obj":"https://acgg.asia/db/ggdb/info/gg106"},{"id":"PD-GlycoGenes20190927-B_T6","span":{"begin":162,"end":203},"obj":"https://acgg.asia/db/ggdb/info/gg105"},{"id":"PD-GlycoGenes20190927-B_T7","span":{"begin":474,"end":476},"obj":"https://acgg.asia/db/ggdb/info/gg111"}],"text":"Molecular cloning and characterization of beta1,4-N-acetylgalactosaminyltransferases IV synthesizing N,N'-diacetyllactosediamine.\nA sequence highly homologous to beta1,4-N-acetylgalactosaminyltransferase III (beta4GalNAc-T3) was found in a database of human expressed sequence tags. The full-length open reading frame of the gene, beta4GalNAc-T4 (GenBank accession number AB089939), was cloned using the 5' rapid amplification of cDNA ends method. It encodes a typical type II transmembrane protein of 1039 amino acids having 42.6% identity with beta4GalNAc-T3. The recombinant enzyme transferred N-acetylgalactosamine to N-acetylglucosamine-beta-benzyl with a beta1,4-linkage to form N,N'-diacetyllactosediamine as did beta4GalNAc-T3. In specificity toward oligosaccharide acceptor substrates, it was quite similar to beta4GalNAc-T3 in vitro, however, the tissue distributions of the two enzymes were quite different. These results indicated that the two enzymes have similar roles in different tissues."}