PubMed:14769797 JSONTXT

{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":75},"obj":"Sentence"},{"id":"T2","span":{"begin":76,"end":167},"obj":"Sentence"},{"id":"T3","span":{"begin":168,"end":342},"obj":"Sentence"},{"id":"T4","span":{"begin":343,"end":464},"obj":"Sentence"},{"id":"T5","span":{"begin":465,"end":658},"obj":"Sentence"},{"id":"T6","span":{"begin":659,"end":771},"obj":"Sentence"},{"id":"T7","span":{"begin":772,"end":920},"obj":"Sentence"},{"id":"T8","span":{"begin":921,"end":1101},"obj":"Sentence"},{"id":"T9","span":{"begin":1102,"end":1279},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Identification of a ZU5 and death domain-containing inhibitor of NF-kappaB.\nThe transcription factor NF-kappaB plays important roles in inflammation and cell survival. NF-kappaB is composed of homodimeric and heterodimeric complexes of Rel/NF-kappaB family members, including p65 (RelA), c-Rel (Rel), RelB, NF-kappaB1/p50, and NF-kappaB2/p52. Here we report the identification and characterization of a novel ZU5 and death domain-containing protein designated ZUD. In reporter gene assays, overexpression of ZUD inhibited NF-kappaB-dependent transcription induced by both tumor necrosis factor (TNF) and interleukin-1 and their downstream signaling proteins. Gel shift assays indicated that the overexpression of ZUD inhibited binding of NF-kappaB to its target sequence. ZUD is a cytoplasmic protein, and coimmunoprecipitation assays indicated that ZUD interacted with the NF-kappaB subunit p105 and transactivator p65. Consistent with its role in inhibition of NF-kappaB-dependent transcription, ZUD sensitized cells to apoptosis induced by TNF and the TNF-related apoptosis-inducing ligand (TRAIL). Our findings suggest that ZUD is an inhibitor of NF-kappaB activation and that this protein may provide an alternative regulatory mechanism for NF-kappaB-mediated transcription."}

{"project":"Inflammaging","denotations":[{"id":"T1","span":{"begin":0,"end":75},"obj":"Sentence"},{"id":"T2","span":{"begin":76,"end":167},"obj":"Sentence"},{"id":"T3","span":{"begin":168,"end":342},"obj":"Sentence"},{"id":"T4","span":{"begin":343,"end":464},"obj":"Sentence"},{"id":"T5","span":{"begin":465,"end":658},"obj":"Sentence"},{"id":"T6","span":{"begin":659,"end":771},"obj":"Sentence"},{"id":"T7","span":{"begin":772,"end":920},"obj":"Sentence"},{"id":"T8","span":{"begin":921,"end":1101},"obj":"Sentence"},{"id":"T9","span":{"begin":1102,"end":1279},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":75},"obj":"Sentence"},{"id":"T2","span":{"begin":76,"end":167},"obj":"Sentence"},{"id":"T3","span":{"begin":168,"end":342},"obj":"Sentence"},{"id":"T4","span":{"begin":343,"end":464},"obj":"Sentence"},{"id":"T5","span":{"begin":465,"end":658},"obj":"Sentence"},{"id":"T6","span":{"begin":659,"end":771},"obj":"Sentence"},{"id":"T7","span":{"begin":772,"end":920},"obj":"Sentence"},{"id":"T8","span":{"begin":921,"end":1101},"obj":"Sentence"},{"id":"T9","span":{"begin":1102,"end":1279},"obj":"Sentence"}],"text":"Identification of a ZU5 and death domain-containing inhibitor of NF-kappaB.\nThe transcription factor NF-kappaB plays important roles in inflammation and cell survival. NF-kappaB is composed of homodimeric and heterodimeric complexes of Rel/NF-kappaB family members, including p65 (RelA), c-Rel (Rel), RelB, NF-kappaB1/p50, and NF-kappaB2/p52. Here we report the identification and characterization of a novel ZU5 and death domain-containing protein designated ZUD. In reporter gene assays, overexpression of ZUD inhibited NF-kappaB-dependent transcription induced by both tumor necrosis factor (TNF) and interleukin-1 and their downstream signaling proteins. Gel shift assays indicated that the overexpression of ZUD inhibited binding of NF-kappaB to its target sequence. ZUD is a cytoplasmic protein, and coimmunoprecipitation assays indicated that ZUD interacted with the NF-kappaB subunit p105 and transactivator p65. Consistent with its role in inhibition of NF-kappaB-dependent transcription, ZUD sensitized cells to apoptosis induced by TNF and the TNF-related apoptosis-inducing ligand (TRAIL). Our findings suggest that ZUD is an inhibitor of NF-kappaB activation and that this protein may provide an alternative regulatory mechanism for NF-kappaB-mediated transcription."}

{"project":"relna","denotations":[{"id":"T1","span":{"begin":236,"end":239},"obj":"DNA"},{"id":"T2","span":{"begin":276,"end":279},"obj":"DNA"},{"id":"T3","span":{"begin":281,"end":285},"obj":"DNA"},{"id":"T4","span":{"begin":288,"end":293},"obj":"DNA"},{"id":"T5","span":{"begin":295,"end":298},"obj":"DNA"},{"id":"T6","span":{"begin":301,"end":305},"obj":"DNA"},{"id":"T7","span":{"begin":318,"end":321},"obj":"DNA"},{"id":"T8","span":{"begin":338,"end":341},"obj":"DNA"},{"id":"T9","span":{"begin":460,"end":463},"obj":"Protein"},{"id":"T10","span":{"begin":508,"end":511},"obj":"Protein"},{"id":"T11","span":{"begin":713,"end":716},"obj":"Protein"},{"id":"T12","span":{"begin":772,"end":775},"obj":"Protein"},{"id":"T13","span":{"begin":850,"end":853},"obj":"Protein"},{"id":"T14","span":{"begin":892,"end":896},"obj":"DNA"},{"id":"T15","span":{"begin":916,"end":919},"obj":"DNA"},{"id":"T16","span":{"begin":998,"end":1001},"obj":"Protein"},{"id":"T17","span":{"begin":1128,"end":1131},"obj":"Protein"}],"relations":[{"id":"R0","pred":"linked","subj":"T13","obj":"T14"},{"id":"R1","pred":"linked","subj":"T13","obj":"T15"}],"text":"Identification of a ZU5 and death domain-containing inhibitor of NF-kappaB.\nThe transcription factor NF-kappaB plays important roles in inflammation and cell survival. NF-kappaB is composed of homodimeric and heterodimeric complexes of Rel/NF-kappaB family members, including p65 (RelA), c-Rel (Rel), RelB, NF-kappaB1/p50, and NF-kappaB2/p52. Here we report the identification and characterization of a novel ZU5 and death domain-containing protein designated ZUD. In reporter gene assays, overexpression of ZUD inhibited NF-kappaB-dependent transcription induced by both tumor necrosis factor (TNF) and interleukin-1 and their downstream signaling proteins. Gel shift assays indicated that the overexpression of ZUD inhibited binding of NF-kappaB to its target sequence. ZUD is a cytoplasmic protein, and coimmunoprecipitation assays indicated that ZUD interacted with the NF-kappaB subunit p105 and transactivator p65. Consistent with its role in inhibition of NF-kappaB-dependent transcription, ZUD sensitized cells to apoptosis induced by TNF and the TNF-related apoptosis-inducing ligand (TRAIL). Our findings suggest that ZUD is an inhibitor of NF-kappaB activation and that this protein may provide an alternative regulatory mechanism for NF-kappaB-mediated transcription."}

{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":572,"end":577},"obj":"HP_0002664"}],"text":"Identification of a ZU5 and death domain-containing inhibitor of NF-kappaB.\nThe transcription factor NF-kappaB plays important roles in inflammation and cell survival. NF-kappaB is composed of homodimeric and heterodimeric complexes of Rel/NF-kappaB family members, including p65 (RelA), c-Rel (Rel), RelB, NF-kappaB1/p50, and NF-kappaB2/p52. Here we report the identification and characterization of a novel ZU5 and death domain-containing protein designated ZUD. In reporter gene assays, overexpression of ZUD inhibited NF-kappaB-dependent transcription induced by both tumor necrosis factor (TNF) and interleukin-1 and their downstream signaling proteins. Gel shift assays indicated that the overexpression of ZUD inhibited binding of NF-kappaB to its target sequence. ZUD is a cytoplasmic protein, and coimmunoprecipitation assays indicated that ZUD interacted with the NF-kappaB subunit p105 and transactivator p65. Consistent with its role in inhibition of NF-kappaB-dependent transcription, ZUD sensitized cells to apoptosis induced by TNF and the TNF-related apoptosis-inducing ligand (TRAIL). Our findings suggest that ZUD is an inhibitor of NF-kappaB activation and that this protein may provide an alternative regulatory mechanism for NF-kappaB-mediated transcription."}

{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":136,"end":148},"obj":"Disease"},{"id":"T2","span":{"begin":572,"end":577},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0021166"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0005070"}],"text":"Identification of a ZU5 and death domain-containing inhibitor of NF-kappaB.\nThe transcription factor NF-kappaB plays important roles in inflammation and cell survival. NF-kappaB is composed of homodimeric and heterodimeric complexes of Rel/NF-kappaB family members, including p65 (RelA), c-Rel (Rel), RelB, NF-kappaB1/p50, and NF-kappaB2/p52. Here we report the identification and characterization of a novel ZU5 and death domain-containing protein designated ZUD. In reporter gene assays, overexpression of ZUD inhibited NF-kappaB-dependent transcription induced by both tumor necrosis factor (TNF) and interleukin-1 and their downstream signaling proteins. Gel shift assays indicated that the overexpression of ZUD inhibited binding of NF-kappaB to its target sequence. ZUD is a cytoplasmic protein, and coimmunoprecipitation assays indicated that ZUD interacted with the NF-kappaB subunit p105 and transactivator p65. Consistent with its role in inhibition of NF-kappaB-dependent transcription, ZUD sensitized cells to apoptosis induced by TNF and the TNF-related apoptosis-inducing ligand (TRAIL). Our findings suggest that ZUD is an inhibitor of NF-kappaB activation and that this protein may provide an alternative regulatory mechanism for NF-kappaB-mediated transcription."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":781,"end":792},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/GO_0005737"}],"text":"Identification of a ZU5 and death domain-containing inhibitor of NF-kappaB.\nThe transcription factor NF-kappaB plays important roles in inflammation and cell survival. NF-kappaB is composed of homodimeric and heterodimeric complexes of Rel/NF-kappaB family members, including p65 (RelA), c-Rel (Rel), RelB, NF-kappaB1/p50, and NF-kappaB2/p52. Here we report the identification and characterization of a novel ZU5 and death domain-containing protein designated ZUD. In reporter gene assays, overexpression of ZUD inhibited NF-kappaB-dependent transcription induced by both tumor necrosis factor (TNF) and interleukin-1 and their downstream signaling proteins. Gel shift assays indicated that the overexpression of ZUD inhibited binding of NF-kappaB to its target sequence. ZUD is a cytoplasmic protein, and coimmunoprecipitation assays indicated that ZUD interacted with the NF-kappaB subunit p105 and transactivator p65. Consistent with its role in inhibition of NF-kappaB-dependent transcription, ZUD sensitized cells to apoptosis induced by TNF and the TNF-related apoptosis-inducing ligand (TRAIL). Our findings suggest that ZUD is an inhibitor of NF-kappaB activation and that this protein may provide an alternative regulatory mechanism for NF-kappaB-mediated transcription."}

{"project":"GlyCosmos15-HP","denotations":[{"id":"T1","span":{"begin":572,"end":577},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0002664"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}],"text":"Identification of a ZU5 and death domain-containing inhibitor of NF-kappaB.\nThe transcription factor NF-kappaB plays important roles in inflammation and cell survival. NF-kappaB is composed of homodimeric and heterodimeric complexes of Rel/NF-kappaB family members, including p65 (RelA), c-Rel (Rel), RelB, NF-kappaB1/p50, and NF-kappaB2/p52. Here we report the identification and characterization of a novel ZU5 and death domain-containing protein designated ZUD. In reporter gene assays, overexpression of ZUD inhibited NF-kappaB-dependent transcription induced by both tumor necrosis factor (TNF) and interleukin-1 and their downstream signaling proteins. Gel shift assays indicated that the overexpression of ZUD inhibited binding of NF-kappaB to its target sequence. ZUD is a cytoplasmic protein, and coimmunoprecipitation assays indicated that ZUD interacted with the NF-kappaB subunit p105 and transactivator p65. Consistent with its role in inhibition of NF-kappaB-dependent transcription, ZUD sensitized cells to apoptosis induced by TNF and the TNF-related apoptosis-inducing ligand (TRAIL). Our findings suggest that ZUD is an inhibitor of NF-kappaB activation and that this protein may provide an alternative regulatory mechanism for NF-kappaB-mediated transcription."}