PubMed:14707483 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/14707483","sourcedb":"PubMed","sourceid":"14707483","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/14707483","text":"Heparin inhibits the reconstituted plasma membrane Ca(2+)-ATPase from porcine brain synaptosome.\nHeparin has been shown to be involved in the regulation of cellular Ca(2+) by binding to many proteins with high affinity. Here we examined the effects of heparin on the plasma membrane Ca(2+)-ATPase from porcine brain synaptosome. Our results showed that heparin dramatically inhibited the ATP hydrolysis and Ca(2+) uptake in the presence and absence of calmodulin. Together with controlled proteolysis by trypsin, we concluded that the calmodulin-binding domain of the plasma membrane Ca(2+)-ATPase was less important for the heparin inhibition. Excess phosphatidylserine was able to eliminate the heparin inhibition. We observed that Ca(2+) affinity kept no obvious changes, but the ATP affinity of plasma membrane Ca(2+)-ATPase was apparently decreased in the presence of heparin. Our results indicated that heparin had little effects on ATP or Ca(2+) binding sites of the enzyme.","tracks":[{"project":"GlyCosmos6-UBERON","denotations":[{"id":"T1","span":{"begin":35,"end":50},"obj":"Body_part"},{"id":"T2","span":{"begin":78,"end":83},"obj":"Body_part"},{"id":"T4","span":{"begin":267,"end":282},"obj":"Body_part"},{"id":"T5","span":{"begin":310,"end":315},"obj":"Body_part"},{"id":"T7","span":{"begin":568,"end":583},"obj":"Body_part"},{"id":"T8","span":{"begin":799,"end":814},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/GO_0005886"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0000955"},{"id":"A3","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_6110636"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/GO_0005886"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_0000955"},{"id":"A6","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_6110636"},{"id":"A7","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/GO_0005886"},{"id":"A8","pred":"uberon_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/GO_0005886"},{"subj":"T1","pred":"source","obj":"GlyCosmos6-UBERON"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-UBERON"},{"subj":"T4","pred":"source","obj":"GlyCosmos6-UBERON"},{"subj":"T5","pred":"source","obj":"GlyCosmos6-UBERON"},{"subj":"T7","pred":"source","obj":"GlyCosmos6-UBERON"},{"subj":"T8","pred":"source","obj":"GlyCosmos6-UBERON"}]},{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":0,"end":7},"obj":"Glycan_Motif"},{"id":"T3","span":{"begin":97,"end":104},"obj":"Glycan_Motif"},{"id":"T5","span":{"begin":252,"end":259},"obj":"Glycan_Motif"},{"id":"T7","span":{"begin":353,"end":360},"obj":"Glycan_Motif"},{"id":"T9","span":{"begin":625,"end":632},"obj":"Glycan_Motif"},{"id":"T11","span":{"begin":697,"end":704},"obj":"Glycan_Motif"},{"id":"T13","span":{"begin":873,"end":880},"obj":"Glycan_Motif"},{"id":"T15","span":{"begin":909,"end":916},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR"},{"id":"A2","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO"},{"id":"A3","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR"},{"id":"A4","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO"},{"id":"A5","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR"},{"id":"A6","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO"},{"id":"A7","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR"},{"id":"A8","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO"},{"id":"A9","pred":"image","subj":"T9","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR"},{"id":"A10","pred":"image","subj":"T9","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO"},{"id":"A11","pred":"image","subj":"T11","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR"},{"id":"A12","pred":"image","subj":"T11","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO"},{"id":"A13","pred":"image","subj":"T13","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR"},{"id":"A14","pred":"image","subj":"T13","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO"},{"id":"A15","pred":"image","subj":"T15","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR"},{"id":"A16","pred":"image","subj":"T15","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO"},{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T5","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T7","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T9","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T11","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T13","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T15","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"}]},{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":0,"end":7},"obj":"https://glytoucan.org/Structures/Glycans/G00021MO"},{"id":"T2","span":{"begin":0,"end":7},"obj":"https://glytoucan.org/Structures/Glycans/G54161DR"},{"id":"T3","span":{"begin":97,"end":104},"obj":"https://glytoucan.org/Structures/Glycans/G00021MO"},{"id":"T4","span":{"begin":97,"end":104},"obj":"https://glytoucan.org/Structures/Glycans/G54161DR"},{"id":"T5","span":{"begin":252,"end":259},"obj":"https://glytoucan.org/Structures/Glycans/G00021MO"},{"id":"T6","span":{"begin":252,"end":259},"obj":"https://glytoucan.org/Structures/Glycans/G54161DR"},{"id":"T7","span":{"begin":353,"end":360},"obj":"https://glytoucan.org/Structures/Glycans/G00021MO"},{"id":"T8","span":{"begin":353,"end":360},"obj":"https://glytoucan.org/Structures/Glycans/G54161DR"},{"id":"T9","span":{"begin":625,"end":632},"obj":"https://glytoucan.org/Structures/Glycans/G00021MO"},{"id":"T10","span":{"begin":625,"end":632},"obj":"https://glytoucan.org/Structures/Glycans/G54161DR"},{"id":"T11","span":{"begin":697,"end":704},"obj":"https://glytoucan.org/Structures/Glycans/G00021MO"},{"id":"T12","span":{"begin":697,"end":704},"obj":"https://glytoucan.org/Structures/Glycans/G54161DR"},{"id":"T13","span":{"begin":873,"end":880},"obj":"https://glytoucan.org/Structures/Glycans/G00021MO"},{"id":"T14","span":{"begin":873,"end":880},"obj":"https://glytoucan.org/Structures/Glycans/G54161DR"},{"id":"T15","span":{"begin":909,"end":916},"obj":"https://glytoucan.org/Structures/Glycans/G00021MO"},{"id":"T16","span":{"begin":909,"end":916},"obj":"https://glytoucan.org/Structures/Glycans/G54161DR"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T4","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T5","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T6","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T7","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T8","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T9","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T10","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T11","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T12","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T13","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T14","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T15","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T16","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"}]},{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":96},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":97,"end":219},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":220,"end":328},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":329,"end":463},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":464,"end":644},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":645,"end":716},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":717,"end":881},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":882,"end":981},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":96},"obj":"Sentence"},{"id":"T2","span":{"begin":97,"end":219},"obj":"Sentence"},{"id":"T3","span":{"begin":220,"end":328},"obj":"Sentence"},{"id":"T4","span":{"begin":329,"end":463},"obj":"Sentence"},{"id":"T5","span":{"begin":464,"end":644},"obj":"Sentence"},{"id":"T6","span":{"begin":645,"end":716},"obj":"Sentence"},{"id":"T7","span":{"begin":717,"end":881},"obj":"Sentence"},{"id":"T8","span":{"begin":882,"end":981},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"}]},{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":78,"end":83},"obj":"http://purl.obolibrary.org/obo/MAT_0000098"},{"id":"T2","span":{"begin":310,"end":315},"obj":"http://purl.obolibrary.org/obo/MAT_0000098"}],"attributes":[{"subj":"T1","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T2","pred":"source","obj":"Glycosmos6-MAT"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"GlyCosmos6-UBERON","color":"#94ec93","default":true},{"id":"GlyCosmos6-Glycan-Motif-Image","color":"#ec93af"},{"id":"GlyCosmos6-Glycan-Motif-Structure","color":"#93c9ec"},{"id":"sentences","color":"#e3ec93"},{"id":"Glycosmos6-MAT","color":"#db93ec"}]}]}}