PubMed:14613940
Annnotations
GlyCosmos6-Glycan-Motif-Image
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 106-109 | Glycan_Motif | denotes | GM1 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G48558GR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46613JI |
| T3 | 484-488 | Glycan_Motif | denotes | GD1a | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G69277LC|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46677TE |
| T5 | 490-494 | Glycan_Motif | denotes | GD1b | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G97612UN|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G37184KW |
| T7 | 496-500 | Glycan_Motif | denotes | GT1b | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G40183QN|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G05952XV |
| T9 | 518-531 | Glycan_Motif | denotes | paragloboside | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G58896AZ|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G48059CD|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G40585SO |
| T12 | 547-550 | Glycan_Motif | denotes | GM3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G91237TK|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G56516VH |
| T14 | 595-598 | Glycan_Motif | denotes | GM1 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G48558GR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46613JI |
| T16 | 633-636 | Glycan_Motif | denotes | GM2 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G79389NT|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G61168WC |
| T18 | 641-644 | Glycan_Motif | denotes | GM1 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G48558GR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46613JI |
| T20 | 756-760 | Glycan_Motif | denotes | GD1a | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G69277LC|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46677TE |
| T22 | 946-949 | Glycan_Motif | denotes | GM3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G91237TK|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G56516VH |
| T24 | 1060-1064 | Glycan_Motif | denotes | GD1a | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G69277LC|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46677TE |
| T26 | 1066-1069 | Glycan_Motif | denotes | GM1 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G48558GR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46613JI |
| T28 | 1075-1078 | Glycan_Motif | denotes | GM2 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G79389NT|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G61168WC |
| T30 | 1156-1159 | Glycan_Motif | denotes | GM1 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G48558GR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46613JI |
| T32 | 1386-1389 | Glycan_Motif | denotes | GM1 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G48558GR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46613JI |
| T34 | 1394-1397 | Glycan_Motif | denotes | GM2 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G79389NT|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G61168WC |
| T36 | 1526-1530 | Glycan_Motif | denotes | GD1a | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G69277LC|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46677TE |
| T38 | 1584-1588 | Glycan_Motif | denotes | GD1a | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G69277LC|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46677TE |
| T40 | 1601-1604 | Glycan_Motif | denotes | GM3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G91237TK|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G56516VH |
| T42 | 1661-1664 | Glycan_Motif | denotes | GM1 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G48558GR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46613JI |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-59 | Sentence | denotes | Properties of recombinant human cytosolic sialidase HsNEU2. |
| T2 | 60-132 | Sentence | denotes | The enzyme hydrolyzes monomerically dispersed GM1 ganglioside molecules. |
| T3 | 133-283 | Sentence | denotes | Recombinant human cytosolic sialidase (HsNEU2), expressed in Escherichia coli, was purified to homogeneity, and its substrate specificity was studied. |
| T4 | 284-660 | Sentence | denotes | HsNEU2 hydrolyzed 4-methylumbelliferyl alpha-NeuAc, alpha 2-->3 sialyllactose, glycoproteins (fetuin, alpha-acid glycoprotein, transferrin, and bovine submaxillary gland mucin), micellar gangliosides GD1a, GD1b, GT1b, and alpha 2-->3 paragloboside, and vesicular GM3. alpha 2-->6 sialyllactose, colominic acid, GM1 oligosaccharide, whereas micellar GM2 and GM1 were resistant. |
| T5 | 661-846 | Sentence | denotes | The optimal pH was 5.6, kinetics Michaelis-Menten type, V(max) varying from 250 IU/mg protein (GD1a) to 0.7 IU/mg protein (alpha(1)-acid glycoprotein), and K(m) in the millimolar range. |
| T6 | 847-1029 | Sentence | denotes | HsNEU2 was activated by detergents (Triton X-100) only with gangliosidic substrates; the change of GM3 from vesicular to mixed micellar aggregation led to a 8.5-fold V(max) increase. |
| T7 | 1030-1108 | Sentence | denotes | HsNEU2 acted on gangliosides (GD1a, GM1, and GM2) at nanomolar concentrations. |
| T8 | 1109-1373 | Sentence | denotes | With these dispersions (studied in detailed on GM1), where monomers are bound to the tube wall or dilutedly associated (1:2000, mol/mol) to Triton X-100 micelles, the V(max) values were 25 and 72 microIU/mg protein, and K(m) was 10 and 15 x 10(-9) m, respectively. |
| T9 | 1374-1431 | Sentence | denotes | Remarkably, GM1 and GM2 were recognized only as monomers. |
| T10 | 1432-1686 | Sentence | denotes | HsNEU2 worked at pH 7.0 with an efficiency (compared with that at pH 5.6) ranging from 4% (on GD1a) to 64% (on alpha(1)-acid glycoprotein), from 7% (on GD1a) to 45% (on GM3) in the presence of Triton X-100, and from 30 to 40% on GM1 monomeric dispersion. |
| T11 | 1687-1888 | Sentence | denotes | These results show that HsNEU2 differentially recognizes the type of sialosyl linkage, the aglycone part of the substrate, and the supramolecular organization (monomer/micelle/vesicle) of gangliosides. |
| T12 | 1889-1999 | Sentence | denotes | The last ability might be relevant in sialidase interactions with gangliosides under physiological conditions. |
GlyCosmos6-Glycan-Motif-Structure
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 106-109 | https://glytoucan.org/Structures/Glycans/G46613JI | denotes | GM1 |
| T2 | 106-109 | https://glytoucan.org/Structures/Glycans/G48558GR | denotes | GM1 |
| T3 | 484-488 | https://glytoucan.org/Structures/Glycans/G46677TE | denotes | GD1a |
| T4 | 484-488 | https://glytoucan.org/Structures/Glycans/G69277LC | denotes | GD1a |
| T5 | 490-494 | https://glytoucan.org/Structures/Glycans/G37184KW | denotes | GD1b |
| T6 | 490-494 | https://glytoucan.org/Structures/Glycans/G97612UN | denotes | GD1b |
| T7 | 496-500 | https://glytoucan.org/Structures/Glycans/G05952XV | denotes | GT1b |
| T8 | 496-500 | https://glytoucan.org/Structures/Glycans/G40183QN | denotes | GT1b |
| T9 | 518-531 | https://glytoucan.org/Structures/Glycans/G40585SO | denotes | paragloboside |
| T10 | 518-531 | https://glytoucan.org/Structures/Glycans/G48059CD | denotes | paragloboside |
| T11 | 518-531 | https://glytoucan.org/Structures/Glycans/G58896AZ | denotes | paragloboside |
| T12 | 547-550 | https://glytoucan.org/Structures/Glycans/G56516VH | denotes | GM3 |
| T13 | 547-550 | https://glytoucan.org/Structures/Glycans/G91237TK | denotes | GM3 |
| T14 | 595-598 | https://glytoucan.org/Structures/Glycans/G46613JI | denotes | GM1 |
| T15 | 595-598 | https://glytoucan.org/Structures/Glycans/G48558GR | denotes | GM1 |
| T16 | 633-636 | https://glytoucan.org/Structures/Glycans/G61168WC | denotes | GM2 |
| T17 | 633-636 | https://glytoucan.org/Structures/Glycans/G79389NT | denotes | GM2 |
| T18 | 641-644 | https://glytoucan.org/Structures/Glycans/G46613JI | denotes | GM1 |
| T19 | 641-644 | https://glytoucan.org/Structures/Glycans/G48558GR | denotes | GM1 |
| T20 | 756-760 | https://glytoucan.org/Structures/Glycans/G46677TE | denotes | GD1a |
| T21 | 756-760 | https://glytoucan.org/Structures/Glycans/G69277LC | denotes | GD1a |
| T22 | 946-949 | https://glytoucan.org/Structures/Glycans/G56516VH | denotes | GM3 |
| T23 | 946-949 | https://glytoucan.org/Structures/Glycans/G91237TK | denotes | GM3 |
| T24 | 1060-1064 | https://glytoucan.org/Structures/Glycans/G46677TE | denotes | GD1a |
| T25 | 1060-1064 | https://glytoucan.org/Structures/Glycans/G69277LC | denotes | GD1a |
| T26 | 1066-1069 | https://glytoucan.org/Structures/Glycans/G46613JI | denotes | GM1 |
| T27 | 1066-1069 | https://glytoucan.org/Structures/Glycans/G48558GR | denotes | GM1 |
| T28 | 1075-1078 | https://glytoucan.org/Structures/Glycans/G61168WC | denotes | GM2 |
| T29 | 1075-1078 | https://glytoucan.org/Structures/Glycans/G79389NT | denotes | GM2 |
| T30 | 1156-1159 | https://glytoucan.org/Structures/Glycans/G46613JI | denotes | GM1 |
| T31 | 1156-1159 | https://glytoucan.org/Structures/Glycans/G48558GR | denotes | GM1 |
| T32 | 1386-1389 | https://glytoucan.org/Structures/Glycans/G46613JI | denotes | GM1 |
| T33 | 1386-1389 | https://glytoucan.org/Structures/Glycans/G48558GR | denotes | GM1 |
| T34 | 1394-1397 | https://glytoucan.org/Structures/Glycans/G61168WC | denotes | GM2 |
| T35 | 1394-1397 | https://glytoucan.org/Structures/Glycans/G79389NT | denotes | GM2 |
| T36 | 1526-1530 | https://glytoucan.org/Structures/Glycans/G46677TE | denotes | GD1a |
| T37 | 1526-1530 | https://glytoucan.org/Structures/Glycans/G69277LC | denotes | GD1a |
| T38 | 1584-1588 | https://glytoucan.org/Structures/Glycans/G46677TE | denotes | GD1a |
| T39 | 1584-1588 | https://glytoucan.org/Structures/Glycans/G69277LC | denotes | GD1a |
| T40 | 1601-1604 | https://glytoucan.org/Structures/Glycans/G56516VH | denotes | GM3 |
| T41 | 1601-1604 | https://glytoucan.org/Structures/Glycans/G91237TK | denotes | GM3 |
| T42 | 1661-1664 | https://glytoucan.org/Structures/Glycans/G46613JI | denotes | GM1 |
| T43 | 1661-1664 | https://glytoucan.org/Structures/Glycans/G48558GR | denotes | GM1 |
Glycosmos6-MAT
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 448-453 | http://purl.obolibrary.org/obo/MAT_0000021 | denotes | gland |
Glycosmos6-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 106-109 | http://www.glycoepitope.jp/epitopes/EP0050 | denotes | GM1 |
| T2 | 484-488 | http://www.glycoepitope.jp/epitopes/EP0056 | denotes | GD1a |
| T3 | 490-494 | http://www.glycoepitope.jp/epitopes/EP0059 | denotes | GD1b |
| T4 | 496-500 | http://www.glycoepitope.jp/epitopes/EP0067 | denotes | GT1b |
| T5 | 518-531 | http://www.glycoepitope.jp/epitopes/EP0075 | denotes | paragloboside |
| T6 | 595-598 | http://www.glycoepitope.jp/epitopes/EP0050 | denotes | GM1 |
| T7 | 641-644 | http://www.glycoepitope.jp/epitopes/EP0050 | denotes | GM1 |
| T8 | 756-760 | http://www.glycoepitope.jp/epitopes/EP0056 | denotes | GD1a |
| T9 | 1060-1064 | http://www.glycoepitope.jp/epitopes/EP0056 | denotes | GD1a |
| T10 | 1066-1069 | http://www.glycoepitope.jp/epitopes/EP0050 | denotes | GM1 |
| T11 | 1156-1159 | http://www.glycoepitope.jp/epitopes/EP0050 | denotes | GM1 |
| T12 | 1386-1389 | http://www.glycoepitope.jp/epitopes/EP0050 | denotes | GM1 |
| T13 | 1526-1530 | http://www.glycoepitope.jp/epitopes/EP0056 | denotes | GD1a |
| T14 | 1584-1588 | http://www.glycoepitope.jp/epitopes/EP0056 | denotes | GD1a |
| T15 | 1661-1664 | http://www.glycoepitope.jp/epitopes/EP0050 | denotes | GM1 |
Glycan-GlyCosmos
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 106-109 | Glycan | denotes | GM1 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR |
| T2 | 329-334 | Glycan | denotes | NeuAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR |
| T3 | 484-488 | Glycan | denotes | GD1a | https://api.glycosmos.org/wurcs2image/latest/png/binary/G46677TE |
| T4 | 490-494 | Glycan | denotes | GD1b | https://api.glycosmos.org/wurcs2image/latest/png/binary/G37184KW |
| T5 | 496-500 | Glycan | denotes | GT1b | https://api.glycosmos.org/wurcs2image/latest/png/binary/G40183QN |
| T6 | 518-531 | Glycan | denotes | paragloboside | https://api.glycosmos.org/wurcs2image/latest/png/binary/G58896AZ |
| T7 | 547-550 | Glycan | denotes | GM3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G91237TK |
| T8 | 595-598 | Glycan | denotes | GM1 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR |
| T9 | 633-636 | Glycan | denotes | GM2 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT |
| T10 | 641-644 | Glycan | denotes | GM1 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR |
| T11 | 756-760 | Glycan | denotes | GD1a | https://api.glycosmos.org/wurcs2image/latest/png/binary/G46677TE |
| T12 | 946-949 | Glycan | denotes | GM3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G91237TK |
| T13 | 1060-1064 | Glycan | denotes | GD1a | https://api.glycosmos.org/wurcs2image/latest/png/binary/G46677TE |
| T14 | 1066-1069 | Glycan | denotes | GM1 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR |
| T15 | 1075-1078 | Glycan | denotes | GM2 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT |
| T16 | 1156-1159 | Glycan | denotes | GM1 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR |
| T17 | 1386-1389 | Glycan | denotes | GM1 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR |
| T18 | 1394-1397 | Glycan | denotes | GM2 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT |
| T19 | 1526-1530 | Glycan | denotes | GD1a | https://api.glycosmos.org/wurcs2image/latest/png/binary/G46677TE |
| T20 | 1584-1588 | Glycan | denotes | GD1a | https://api.glycosmos.org/wurcs2image/latest/png/binary/G46677TE |
| T21 | 1601-1604 | Glycan | denotes | GM3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G91237TK |
| T22 | 1661-1664 | Glycan | denotes | GM1 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR |
GlyCosmos-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 106-109 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GM1 | http://www.glycoepitope.jp/epitopes/EP0050 |
| T2 | 484-488 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GD1a | http://www.glycoepitope.jp/epitopes/EP0056 |
| T3 | 490-494 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GD1b | http://www.glycoepitope.jp/epitopes/EP0059 |
| T4 | 496-500 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GT1b | http://www.glycoepitope.jp/epitopes/EP0067 |
| T5 | 518-531 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | paragloboside | http://www.glycoepitope.jp/epitopes/EP0075 |
| T6 | 595-598 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GM1 | http://www.glycoepitope.jp/epitopes/EP0050 |
| T7 | 641-644 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GM1 | http://www.glycoepitope.jp/epitopes/EP0050 |
| T8 | 756-760 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GD1a | http://www.glycoepitope.jp/epitopes/EP0056 |
| T9 | 1060-1064 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GD1a | http://www.glycoepitope.jp/epitopes/EP0056 |
| T10 | 1066-1069 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GM1 | http://www.glycoepitope.jp/epitopes/EP0050 |
| T11 | 1156-1159 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GM1 | http://www.glycoepitope.jp/epitopes/EP0050 |
| T12 | 1386-1389 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GM1 | http://www.glycoepitope.jp/epitopes/EP0050 |
| T13 | 1526-1530 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GD1a | http://www.glycoepitope.jp/epitopes/EP0056 |
| T14 | 1584-1588 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GD1a | http://www.glycoepitope.jp/epitopes/EP0056 |
| T15 | 1661-1664 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GM1 | http://www.glycoepitope.jp/epitopes/EP0050 |
GlyCosmos15-NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 26-31 | OrganismTaxon | denotes | human | 9606 |
| T2 | 145-150 | OrganismTaxon | denotes | human | 9606 |
| T3 | 194-210 | OrganismTaxon | denotes | Escherichia coli | 562 |
| T4 | 428-434 | OrganismTaxon | denotes | bovine | 9913 |
GlyCosmos15-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 435-453 | Body_part | denotes | submaxillary gland | http://purl.obolibrary.org/obo/UBERON_0001736|http://purl.obolibrary.org/obo/UBERON_0001832 |
| T3 | 1194-1198 | Body_part | denotes | tube | http://purl.obolibrary.org/obo/UBERON_0000025 |
| T4 | 1199-1203 | Body_part | denotes | wall | http://purl.obolibrary.org/obo/UBERON_0000060 |
| T5 | 1863-1870 | Body_part | denotes | vesicle | http://purl.obolibrary.org/obo/GO_0031982 |
GlyCosmos15-MAT
| Id | Subject | Object | Predicate | Lexical cue | mat_id |
|---|---|---|---|---|---|
| T1 | 448-453 | Body_part | denotes | gland | http://purl.obolibrary.org/obo/MAT_0000021 |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-59 | Sentence | denotes | Properties of recombinant human cytosolic sialidase HsNEU2. |
| T2 | 60-132 | Sentence | denotes | The enzyme hydrolyzes monomerically dispersed GM1 ganglioside molecules. |
| T3 | 133-283 | Sentence | denotes | Recombinant human cytosolic sialidase (HsNEU2), expressed in Escherichia coli, was purified to homogeneity, and its substrate specificity was studied. |
| T4 | 284-660 | Sentence | denotes | HsNEU2 hydrolyzed 4-methylumbelliferyl alpha-NeuAc, alpha 2-->3 sialyllactose, glycoproteins (fetuin, alpha-acid glycoprotein, transferrin, and bovine submaxillary gland mucin), micellar gangliosides GD1a, GD1b, GT1b, and alpha 2-->3 paragloboside, and vesicular GM3. alpha 2-->6 sialyllactose, colominic acid, GM1 oligosaccharide, whereas micellar GM2 and GM1 were resistant. |
| T5 | 661-846 | Sentence | denotes | The optimal pH was 5.6, kinetics Michaelis-Menten type, V(max) varying from 250 IU/mg protein (GD1a) to 0.7 IU/mg protein (alpha(1)-acid glycoprotein), and K(m) in the millimolar range. |
| T6 | 847-1029 | Sentence | denotes | HsNEU2 was activated by detergents (Triton X-100) only with gangliosidic substrates; the change of GM3 from vesicular to mixed micellar aggregation led to a 8.5-fold V(max) increase. |
| T7 | 1030-1108 | Sentence | denotes | HsNEU2 acted on gangliosides (GD1a, GM1, and GM2) at nanomolar concentrations. |
| T8 | 1109-1373 | Sentence | denotes | With these dispersions (studied in detailed on GM1), where monomers are bound to the tube wall or dilutedly associated (1:2000, mol/mol) to Triton X-100 micelles, the V(max) values were 25 and 72 microIU/mg protein, and K(m) was 10 and 15 x 10(-9) m, respectively. |
| T9 | 1374-1431 | Sentence | denotes | Remarkably, GM1 and GM2 were recognized only as monomers. |
| T10 | 1432-1686 | Sentence | denotes | HsNEU2 worked at pH 7.0 with an efficiency (compared with that at pH 5.6) ranging from 4% (on GD1a) to 64% (on alpha(1)-acid glycoprotein), from 7% (on GD1a) to 45% (on GM3) in the presence of Triton X-100, and from 30 to 40% on GM1 monomeric dispersion. |
| T11 | 1687-1888 | Sentence | denotes | These results show that HsNEU2 differentially recognizes the type of sialosyl linkage, the aglycone part of the substrate, and the supramolecular organization (monomer/micelle/vesicle) of gangliosides. |
| T12 | 1889-1999 | Sentence | denotes | The last ability might be relevant in sialidase interactions with gangliosides under physiological conditions. |
GlyCosmos15-Sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-59 | Sentence | denotes | Properties of recombinant human cytosolic sialidase HsNEU2. |
| T2 | 60-132 | Sentence | denotes | The enzyme hydrolyzes monomerically dispersed GM1 ganglioside molecules. |
| T3 | 133-283 | Sentence | denotes | Recombinant human cytosolic sialidase (HsNEU2), expressed in Escherichia coli, was purified to homogeneity, and its substrate specificity was studied. |
| T4 | 284-660 | Sentence | denotes | HsNEU2 hydrolyzed 4-methylumbelliferyl alpha-NeuAc, alpha 2-->3 sialyllactose, glycoproteins (fetuin, alpha-acid glycoprotein, transferrin, and bovine submaxillary gland mucin), micellar gangliosides GD1a, GD1b, GT1b, and alpha 2-->3 paragloboside, and vesicular GM3. alpha 2-->6 sialyllactose, colominic acid, GM1 oligosaccharide, whereas micellar GM2 and GM1 were resistant. |
| T5 | 661-846 | Sentence | denotes | The optimal pH was 5.6, kinetics Michaelis-Menten type, V(max) varying from 250 IU/mg protein (GD1a) to 0.7 IU/mg protein (alpha(1)-acid glycoprotein), and K(m) in the millimolar range. |
| T6 | 847-1029 | Sentence | denotes | HsNEU2 was activated by detergents (Triton X-100) only with gangliosidic substrates; the change of GM3 from vesicular to mixed micellar aggregation led to a 8.5-fold V(max) increase. |
| T7 | 1030-1108 | Sentence | denotes | HsNEU2 acted on gangliosides (GD1a, GM1, and GM2) at nanomolar concentrations. |
| T8 | 1109-1373 | Sentence | denotes | With these dispersions (studied in detailed on GM1), where monomers are bound to the tube wall or dilutedly associated (1:2000, mol/mol) to Triton X-100 micelles, the V(max) values were 25 and 72 microIU/mg protein, and K(m) was 10 and 15 x 10(-9) m, respectively. |
| T9 | 1374-1431 | Sentence | denotes | Remarkably, GM1 and GM2 were recognized only as monomers. |
| T10 | 1432-1686 | Sentence | denotes | HsNEU2 worked at pH 7.0 with an efficiency (compared with that at pH 5.6) ranging from 4% (on GD1a) to 64% (on alpha(1)-acid glycoprotein), from 7% (on GD1a) to 45% (on GM3) in the presence of Triton X-100, and from 30 to 40% on GM1 monomeric dispersion. |
| T11 | 1687-1888 | Sentence | denotes | These results show that HsNEU2 differentially recognizes the type of sialosyl linkage, the aglycone part of the substrate, and the supramolecular organization (monomer/micelle/vesicle) of gangliosides. |
| T12 | 1889-1999 | Sentence | denotes | The last ability might be relevant in sialidase interactions with gangliosides under physiological conditions. |
GlyCosmos15-Glycan
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 106-109 | Glycan | denotes | GM1 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR |
| T2 | 329-334 | Glycan | denotes | NeuAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR |
| T3 | 484-488 | Glycan | denotes | GD1a | https://api.glycosmos.org/wurcs2image/latest/png/binary/G46677TE |
| T4 | 490-494 | Glycan | denotes | GD1b | https://api.glycosmos.org/wurcs2image/latest/png/binary/G37184KW |
| T5 | 496-500 | Glycan | denotes | GT1b | https://api.glycosmos.org/wurcs2image/latest/png/binary/G40183QN |
| T6 | 518-531 | Glycan | denotes | paragloboside | https://api.glycosmos.org/wurcs2image/latest/png/binary/G58896AZ |
| T7 | 547-550 | Glycan | denotes | GM3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G91237TK |
| T8 | 595-598 | Glycan | denotes | GM1 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR |
| T9 | 633-636 | Glycan | denotes | GM2 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT |
| T10 | 641-644 | Glycan | denotes | GM1 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR |
| T11 | 756-760 | Glycan | denotes | GD1a | https://api.glycosmos.org/wurcs2image/latest/png/binary/G46677TE |
| T12 | 946-949 | Glycan | denotes | GM3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G91237TK |
| T13 | 1060-1064 | Glycan | denotes | GD1a | https://api.glycosmos.org/wurcs2image/latest/png/binary/G46677TE |
| T14 | 1066-1069 | Glycan | denotes | GM1 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR |
| T15 | 1075-1078 | Glycan | denotes | GM2 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT |
| T16 | 1156-1159 | Glycan | denotes | GM1 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR |
| T17 | 1386-1389 | Glycan | denotes | GM1 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR |
| T18 | 1394-1397 | Glycan | denotes | GM2 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT |
| T19 | 1526-1530 | Glycan | denotes | GD1a | https://api.glycosmos.org/wurcs2image/latest/png/binary/G46677TE |
| T20 | 1584-1588 | Glycan | denotes | GD1a | https://api.glycosmos.org/wurcs2image/latest/png/binary/G46677TE |
| T21 | 1601-1604 | Glycan | denotes | GM3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G91237TK |
| T22 | 1661-1664 | Glycan | denotes | GM1 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR |
GlyCosmos15-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 106-109 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GM1 | http://www.glycoepitope.jp/epitopes/EP0050 |
| T2 | 484-488 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GD1a | http://www.glycoepitope.jp/epitopes/EP0056 |
| T3 | 490-494 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GD1b | http://www.glycoepitope.jp/epitopes/EP0059 |
| T4 | 496-500 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GT1b | http://www.glycoepitope.jp/epitopes/EP0067 |
| T5 | 518-531 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | paragloboside | http://www.glycoepitope.jp/epitopes/EP0075 |
| T6 | 595-598 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GM1 | http://www.glycoepitope.jp/epitopes/EP0050 |
| T7 | 641-644 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GM1 | http://www.glycoepitope.jp/epitopes/EP0050 |
| T8 | 756-760 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GD1a | http://www.glycoepitope.jp/epitopes/EP0056 |
| T9 | 1060-1064 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GD1a | http://www.glycoepitope.jp/epitopes/EP0056 |
| T10 | 1066-1069 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GM1 | http://www.glycoepitope.jp/epitopes/EP0050 |
| T11 | 1156-1159 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GM1 | http://www.glycoepitope.jp/epitopes/EP0050 |
| T12 | 1386-1389 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GM1 | http://www.glycoepitope.jp/epitopes/EP0050 |
| T13 | 1526-1530 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GD1a | http://www.glycoepitope.jp/epitopes/EP0056 |
| T14 | 1584-1588 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GD1a | http://www.glycoepitope.jp/epitopes/EP0056 |
| T15 | 1661-1664 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | GM1 | http://www.glycoepitope.jp/epitopes/EP0050 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 26-31 | OrganismTaxon | denotes | human | 9606 |
| T2 | 145-150 | OrganismTaxon | denotes | human | 9606 |
| T3 | 194-210 | OrganismTaxon | denotes | Escherichia coli | 562 |
| T4 | 428-434 | OrganismTaxon | denotes | bovine | 9913 |
Anatomy-MAT
| Id | Subject | Object | Predicate | Lexical cue | mat_id |
|---|---|---|---|---|---|
| T1 | 448-453 | Body_part | denotes | gland | http://purl.obolibrary.org/obo/MAT_0000021 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 435-453 | Body_part | denotes | submaxillary gland | http://purl.obolibrary.org/obo/UBERON_0001736|http://purl.obolibrary.org/obo/UBERON_0001832 |
| T3 | 1194-1198 | Body_part | denotes | tube | http://purl.obolibrary.org/obo/UBERON_0000025 |
| T4 | 1199-1203 | Body_part | denotes | wall | http://purl.obolibrary.org/obo/UBERON_0000060 |
| T5 | 1863-1870 | Body_part | denotes | vesicle | http://purl.obolibrary.org/obo/GO_0031982 |