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GlyCosmos6-Glycan-Motif-Image

sentences

{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":60},"obj":"Sentence"},{"id":"T2","span":{"begin":61,"end":114},"obj":"Sentence"},{"id":"T3","span":{"begin":115,"end":226},"obj":"Sentence"},{"id":"T4","span":{"begin":227,"end":377},"obj":"Sentence"},{"id":"T5","span":{"begin":378,"end":463},"obj":"Sentence"},{"id":"T6","span":{"begin":464,"end":615},"obj":"Sentence"},{"id":"T7","span":{"begin":616,"end":788},"obj":"Sentence"},{"id":"T8","span":{"begin":789,"end":936},"obj":"Sentence"},{"id":"T9","span":{"begin":937,"end":1039},"obj":"Sentence"},{"id":"T10","span":{"begin":1040,"end":1196},"obj":"Sentence"},{"id":"T11","span":{"begin":1197,"end":1311},"obj":"Sentence"},{"id":"T12","span":{"begin":1312,"end":1432},"obj":"Sentence"},{"id":"T13","span":{"begin":1433,"end":1559},"obj":"Sentence"},{"id":"T14","span":{"begin":1560,"end":1808},"obj":"Sentence"},{"id":"T15","span":{"begin":1809,"end":1958},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":60},"obj":"Sentence"},{"id":"T2","span":{"begin":61,"end":114},"obj":"Sentence"},{"id":"T3","span":{"begin":115,"end":226},"obj":"Sentence"},{"id":"T4","span":{"begin":227,"end":377},"obj":"Sentence"},{"id":"T5","span":{"begin":378,"end":463},"obj":"Sentence"},{"id":"T6","span":{"begin":464,"end":615},"obj":"Sentence"},{"id":"T7","span":{"begin":616,"end":788},"obj":"Sentence"},{"id":"T8","span":{"begin":789,"end":936},"obj":"Sentence"},{"id":"T9","span":{"begin":937,"end":1039},"obj":"Sentence"},{"id":"T10","span":{"begin":1040,"end":1196},"obj":"Sentence"},{"id":"T11","span":{"begin":1197,"end":1311},"obj":"Sentence"},{"id":"T12","span":{"begin":1312,"end":1432},"obj":"Sentence"},{"id":"T13","span":{"begin":1433,"end":1559},"obj":"Sentence"},{"id":"T14","span":{"begin":1560,"end":1808},"obj":"Sentence"},{"id":"T15","span":{"begin":1809,"end":1958},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Poly-N-acetyllactosaminyl O-glycans attached to leukosialin. The presence of sialyl Le(x) structures in O-glycans.\nPoly-N-acetyllactosamine extension has been found in O-glycans in addition to N-glycans and glycosphingolipids. Attempts were made in HL-60 and K562 cells to determine the amount of poly-N-acetyllactosaminyl O-glycans in the major sialoglycoprotein, leukosialin. Leukosialin was immunoprecipitated from [3H]glucosamine-labeled HL-60 and K562 cells. Glycopeptides were prepared by Pronase digestion, and O-glycan-containing glycopeptides were isolated by affinity chromatography using Jacalin-agarose. The glycopeptides bound to Jacalin-agarose and those unbound were treated with alkaline borohydride, and the released O-glycans were fractionated by Bio-Gel P-4 filtration. Sequential glycosidase digestion of the O-glycans, with or without pretreatment by fucosidase or neuraminidase, revealed the following conclusions. 1) Leukosialin from HL-60 cells contains about 1-2 poly-N-acetyllactosaminyl O-glycan chains/molecule. 2) About 50% of these poly-N-acetyllactosaminyl O-glycans contain sialyl Le(x) termini, NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 (Fuc alpha 1--\u003e3)GlcNAc beta 1--\u003eR. The amount of sialyl Le(x) structure in leukosialin is roughly equivalent to that on cell surfaces of HL-60 cells. 3) Leukosialin from K562 cells, on the other hand, contains no detectable amount of poly-N-acetyllactosaminyl O-glycans. 4) The presence of poly-N-acetyllactosamine in O-glycans is dependent on the core 2 beta 1,6-N-acetylglucosaminyl transferase. 5) Jacalin-agarose binds to sialylated small oligosaccharides such as NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e6) GalNAc but not the hexasaccharide NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4GlcNAc beta 1--\u003e6) GalNAc. These results indicate that the formation of polylactosaminyl O-glycans and sialyl Le(x) structure in O-glycans is dependent on the core 2 formation."}

GlyCosmos6-Glycan-Motif-Structure

{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":48,"end":59},"obj":"https://glytoucan.org/Structures/Glycans/G50850NI"},{"id":"T2","span":{"begin":48,"end":59},"obj":"https://glytoucan.org/Structures/Glycans/G78436BB"},{"id":"T3","span":{"begin":84,"end":89},"obj":"https://glytoucan.org/Structures/Glycans/G01187XC"},{"id":"T4","span":{"begin":120,"end":139},"obj":"https://glytoucan.org/Structures/Glycans/G00055MO"},{"id":"T5","span":{"begin":365,"end":376},"obj":"https://glytoucan.org/Structures/Glycans/G50850NI"},{"id":"T6","span":{"begin":365,"end":376},"obj":"https://glytoucan.org/Structures/Glycans/G78436BB"},{"id":"T7","span":{"begin":378,"end":389},"obj":"https://glytoucan.org/Structures/Glycans/G50850NI"},{"id":"T8","span":{"begin":378,"end":389},"obj":"https://glytoucan.org/Structures/Glycans/G78436BB"},{"id":"T9","span":{"begin":940,"end":951},"obj":"https://glytoucan.org/Structures/Glycans/G50850NI"},{"id":"T10","span":{"begin":940,"end":951},"obj":"https://glytoucan.org/Structures/Glycans/G78436BB"},{"id":"T11","span":{"begin":1113,"end":1118},"obj":"https://glytoucan.org/Structures/Glycans/G01187XC"},{"id":"T12","span":{"begin":1218,"end":1223},"obj":"https://glytoucan.org/Structures/Glycans/G01187XC"},{"id":"T13","span":{"begin":1237,"end":1248},"obj":"https://glytoucan.org/Structures/Glycans/G50850NI"},{"id":"T14","span":{"begin":1237,"end":1248},"obj":"https://glytoucan.org/Structures/Glycans/G78436BB"},{"id":"T15","span":{"begin":1315,"end":1326},"obj":"https://glytoucan.org/Structures/Glycans/G50850NI"},{"id":"T16","span":{"begin":1315,"end":1326},"obj":"https://glytoucan.org/Structures/Glycans/G78436BB"},{"id":"T17","span":{"begin":1457,"end":1476},"obj":"https://glytoucan.org/Structures/Glycans/G00055MO"},{"id":"T18","span":{"begin":1892,"end":1897},"obj":"https://glytoucan.org/Structures/Glycans/G01187XC"}],"text":"Poly-N-acetyllactosaminyl O-glycans attached to leukosialin. The presence of sialyl Le(x) structures in O-glycans.\nPoly-N-acetyllactosamine extension has been found in O-glycans in addition to N-glycans and glycosphingolipids. Attempts were made in HL-60 and K562 cells to determine the amount of poly-N-acetyllactosaminyl O-glycans in the major sialoglycoprotein, leukosialin. Leukosialin was immunoprecipitated from [3H]glucosamine-labeled HL-60 and K562 cells. Glycopeptides were prepared by Pronase digestion, and O-glycan-containing glycopeptides were isolated by affinity chromatography using Jacalin-agarose. The glycopeptides bound to Jacalin-agarose and those unbound were treated with alkaline borohydride, and the released O-glycans were fractionated by Bio-Gel P-4 filtration. Sequential glycosidase digestion of the O-glycans, with or without pretreatment by fucosidase or neuraminidase, revealed the following conclusions. 1) Leukosialin from HL-60 cells contains about 1-2 poly-N-acetyllactosaminyl O-glycan chains/molecule. 2) About 50% of these poly-N-acetyllactosaminyl O-glycans contain sialyl Le(x) termini, NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 (Fuc alpha 1--\u003e3)GlcNAc beta 1--\u003eR. The amount of sialyl Le(x) structure in leukosialin is roughly equivalent to that on cell surfaces of HL-60 cells. 3) Leukosialin from K562 cells, on the other hand, contains no detectable amount of poly-N-acetyllactosaminyl O-glycans. 4) The presence of poly-N-acetyllactosamine in O-glycans is dependent on the core 2 beta 1,6-N-acetylglucosaminyl transferase. 5) Jacalin-agarose binds to sialylated small oligosaccharides such as NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e6) GalNAc but not the hexasaccharide NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4GlcNAc beta 1--\u003e6) GalNAc. These results indicate that the formation of polylactosaminyl O-glycans and sialyl Le(x) structure in O-glycans is dependent on the core 2 formation."}

Glycosmos6-MAT

{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":1357,"end":1361},"obj":"http://purl.obolibrary.org/obo/MAT_0000091"}],"text":"Poly-N-acetyllactosaminyl O-glycans attached to leukosialin. The presence of sialyl Le(x) structures in O-glycans.\nPoly-N-acetyllactosamine extension has been found in O-glycans in addition to N-glycans and glycosphingolipids. Attempts were made in HL-60 and K562 cells to determine the amount of poly-N-acetyllactosaminyl O-glycans in the major sialoglycoprotein, leukosialin. Leukosialin was immunoprecipitated from [3H]glucosamine-labeled HL-60 and K562 cells. Glycopeptides were prepared by Pronase digestion, and O-glycan-containing glycopeptides were isolated by affinity chromatography using Jacalin-agarose. The glycopeptides bound to Jacalin-agarose and those unbound were treated with alkaline borohydride, and the released O-glycans were fractionated by Bio-Gel P-4 filtration. Sequential glycosidase digestion of the O-glycans, with or without pretreatment by fucosidase or neuraminidase, revealed the following conclusions. 1) Leukosialin from HL-60 cells contains about 1-2 poly-N-acetyllactosaminyl O-glycan chains/molecule. 2) About 50% of these poly-N-acetyllactosaminyl O-glycans contain sialyl Le(x) termini, NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 (Fuc alpha 1--\u003e3)GlcNAc beta 1--\u003eR. The amount of sialyl Le(x) structure in leukosialin is roughly equivalent to that on cell surfaces of HL-60 cells. 3) Leukosialin from K562 cells, on the other hand, contains no detectable amount of poly-N-acetyllactosaminyl O-glycans. 4) The presence of poly-N-acetyllactosamine in O-glycans is dependent on the core 2 beta 1,6-N-acetylglucosaminyl transferase. 5) Jacalin-agarose binds to sialylated small oligosaccharides such as NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e6) GalNAc but not the hexasaccharide NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4GlcNAc beta 1--\u003e6) GalNAc. These results indicate that the formation of polylactosaminyl O-glycans and sialyl Le(x) structure in O-glycans is dependent on the core 2 formation."}

NGLY1-deficiency

{"project":"NGLY1-deficiency","denotations":[{"id":"PD-NGLY1-deficiency-B_T1","span":{"begin":1178,"end":1184},"obj":"chem:24139"},{"id":"PD-NGLY1-deficiency-B_T2","span":{"begin":1526,"end":1546},"obj":"chem:24139"}],"namespaces":[{"prefix":"hgnc","uri":"https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:"},{"prefix":"omim","uri":"https://www.omim.org/entry/"},{"prefix":"chem","uri":"https://pubchem.ncbi.nlm.nih.gov/compound/"}],"text":"Poly-N-acetyllactosaminyl O-glycans attached to leukosialin. The presence of sialyl Le(x) structures in O-glycans.\nPoly-N-acetyllactosamine extension has been found in O-glycans in addition to N-glycans and glycosphingolipids. Attempts were made in HL-60 and K562 cells to determine the amount of poly-N-acetyllactosaminyl O-glycans in the major sialoglycoprotein, leukosialin. Leukosialin was immunoprecipitated from [3H]glucosamine-labeled HL-60 and K562 cells. Glycopeptides were prepared by Pronase digestion, and O-glycan-containing glycopeptides were isolated by affinity chromatography using Jacalin-agarose. The glycopeptides bound to Jacalin-agarose and those unbound were treated with alkaline borohydride, and the released O-glycans were fractionated by Bio-Gel P-4 filtration. Sequential glycosidase digestion of the O-glycans, with or without pretreatment by fucosidase or neuraminidase, revealed the following conclusions. 1) Leukosialin from HL-60 cells contains about 1-2 poly-N-acetyllactosaminyl O-glycan chains/molecule. 2) About 50% of these poly-N-acetyllactosaminyl O-glycans contain sialyl Le(x) termini, NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 (Fuc alpha 1--\u003e3)GlcNAc beta 1--\u003eR. The amount of sialyl Le(x) structure in leukosialin is roughly equivalent to that on cell surfaces of HL-60 cells. 3) Leukosialin from K562 cells, on the other hand, contains no detectable amount of poly-N-acetyllactosaminyl O-glycans. 4) The presence of poly-N-acetyllactosamine in O-glycans is dependent on the core 2 beta 1,6-N-acetylglucosaminyl transferase. 5) Jacalin-agarose binds to sialylated small oligosaccharides such as NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e6) GalNAc but not the hexasaccharide NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4GlcNAc beta 1--\u003e6) GalNAc. These results indicate that the formation of polylactosaminyl O-glycans and sialyl Le(x) structure in O-glycans is dependent on the core 2 formation."}

Anatomy-MAT

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":1357,"end":1361},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000091"}],"text":"Poly-N-acetyllactosaminyl O-glycans attached to leukosialin. The presence of sialyl Le(x) structures in O-glycans.\nPoly-N-acetyllactosamine extension has been found in O-glycans in addition to N-glycans and glycosphingolipids. Attempts were made in HL-60 and K562 cells to determine the amount of poly-N-acetyllactosaminyl O-glycans in the major sialoglycoprotein, leukosialin. Leukosialin was immunoprecipitated from [3H]glucosamine-labeled HL-60 and K562 cells. Glycopeptides were prepared by Pronase digestion, and O-glycan-containing glycopeptides were isolated by affinity chromatography using Jacalin-agarose. The glycopeptides bound to Jacalin-agarose and those unbound were treated with alkaline borohydride, and the released O-glycans were fractionated by Bio-Gel P-4 filtration. Sequential glycosidase digestion of the O-glycans, with or without pretreatment by fucosidase or neuraminidase, revealed the following conclusions. 1) Leukosialin from HL-60 cells contains about 1-2 poly-N-acetyllactosaminyl O-glycan chains/molecule. 2) About 50% of these poly-N-acetyllactosaminyl O-glycans contain sialyl Le(x) termini, NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 (Fuc alpha 1--\u003e3)GlcNAc beta 1--\u003eR. The amount of sialyl Le(x) structure in leukosialin is roughly equivalent to that on cell surfaces of HL-60 cells. 3) Leukosialin from K562 cells, on the other hand, contains no detectable amount of poly-N-acetyllactosaminyl O-glycans. 4) The presence of poly-N-acetyllactosamine in O-glycans is dependent on the core 2 beta 1,6-N-acetylglucosaminyl transferase. 5) Jacalin-agarose binds to sialylated small oligosaccharides such as NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e6) GalNAc but not the hexasaccharide NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4GlcNAc beta 1--\u003e6) GalNAc. These results indicate that the formation of polylactosaminyl O-glycans and sialyl Le(x) structure in O-glycans is dependent on the core 2 formation."}

Glycan-GlyCosmos

GlyCosmos15-CL

{"project":"GlyCosmos15-CL","denotations":[{"id":"T1","span":{"begin":0,"end":4},"obj":"Cell"},{"id":"T3","span":{"begin":115,"end":119},"obj":"Cell"},{"id":"T5","span":{"begin":297,"end":301},"obj":"Cell"},{"id":"T7","span":{"begin":988,"end":992},"obj":"Cell"},{"id":"T9","span":{"begin":1062,"end":1066},"obj":"Cell"},{"id":"T11","span":{"begin":1396,"end":1400},"obj":"Cell"},{"id":"T13","span":{"begin":1452,"end":1456},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000096"},{"id":"A2","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000775"},{"id":"A3","pred":"cl_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL:0000096"},{"id":"A4","pred":"cl_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL:0000775"},{"id":"A5","pred":"cl_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CL:0000096"},{"id":"A6","pred":"cl_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CL:0000775"},{"id":"A7","pred":"cl_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/CL:0000096"},{"id":"A8","pred":"cl_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/CL:0000775"},{"id":"A9","pred":"cl_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/CL:0000096"},{"id":"A10","pred":"cl_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/CL:0000775"},{"id":"A11","pred":"cl_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/CL:0000096"},{"id":"A12","pred":"cl_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/CL:0000775"},{"id":"A13","pred":"cl_id","subj":"T13","obj":"http://purl.obolibrary.org/obo/CL:0000096"},{"id":"A14","pred":"cl_id","subj":"T13","obj":"http://purl.obolibrary.org/obo/CL:0000775"}],"text":"Poly-N-acetyllactosaminyl O-glycans attached to leukosialin. The presence of sialyl Le(x) structures in O-glycans.\nPoly-N-acetyllactosamine extension has been found in O-glycans in addition to N-glycans and glycosphingolipids. Attempts were made in HL-60 and K562 cells to determine the amount of poly-N-acetyllactosaminyl O-glycans in the major sialoglycoprotein, leukosialin. Leukosialin was immunoprecipitated from [3H]glucosamine-labeled HL-60 and K562 cells. Glycopeptides were prepared by Pronase digestion, and O-glycan-containing glycopeptides were isolated by affinity chromatography using Jacalin-agarose. The glycopeptides bound to Jacalin-agarose and those unbound were treated with alkaline borohydride, and the released O-glycans were fractionated by Bio-Gel P-4 filtration. Sequential glycosidase digestion of the O-glycans, with or without pretreatment by fucosidase or neuraminidase, revealed the following conclusions. 1) Leukosialin from HL-60 cells contains about 1-2 poly-N-acetyllactosaminyl O-glycan chains/molecule. 2) About 50% of these poly-N-acetyllactosaminyl O-glycans contain sialyl Le(x) termini, NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 (Fuc alpha 1--\u003e3)GlcNAc beta 1--\u003eR. The amount of sialyl Le(x) structure in leukosialin is roughly equivalent to that on cell surfaces of HL-60 cells. 3) Leukosialin from K562 cells, on the other hand, contains no detectable amount of poly-N-acetyllactosaminyl O-glycans. 4) The presence of poly-N-acetyllactosamine in O-glycans is dependent on the core 2 beta 1,6-N-acetylglucosaminyl transferase. 5) Jacalin-agarose binds to sialylated small oligosaccharides such as NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e6) GalNAc but not the hexasaccharide NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4GlcNAc beta 1--\u003e6) GalNAc. These results indicate that the formation of polylactosaminyl O-glycans and sialyl Le(x) structure in O-glycans is dependent on the core 2 formation."}

GlyCosmos15-UBERON

{"project":"GlyCosmos15-UBERON","denotations":[{"id":"T1","span":{"begin":140,"end":149},"obj":"Body_part"},{"id":"T2","span":{"begin":1357,"end":1361},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_2000106"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0002398"}],"text":"Poly-N-acetyllactosaminyl O-glycans attached to leukosialin. The presence of sialyl Le(x) structures in O-glycans.\nPoly-N-acetyllactosamine extension has been found in O-glycans in addition to N-glycans and glycosphingolipids. Attempts were made in HL-60 and K562 cells to determine the amount of poly-N-acetyllactosaminyl O-glycans in the major sialoglycoprotein, leukosialin. Leukosialin was immunoprecipitated from [3H]glucosamine-labeled HL-60 and K562 cells. Glycopeptides were prepared by Pronase digestion, and O-glycan-containing glycopeptides were isolated by affinity chromatography using Jacalin-agarose. The glycopeptides bound to Jacalin-agarose and those unbound were treated with alkaline borohydride, and the released O-glycans were fractionated by Bio-Gel P-4 filtration. Sequential glycosidase digestion of the O-glycans, with or without pretreatment by fucosidase or neuraminidase, revealed the following conclusions. 1) Leukosialin from HL-60 cells contains about 1-2 poly-N-acetyllactosaminyl O-glycan chains/molecule. 2) About 50% of these poly-N-acetyllactosaminyl O-glycans contain sialyl Le(x) termini, NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 (Fuc alpha 1--\u003e3)GlcNAc beta 1--\u003eR. The amount of sialyl Le(x) structure in leukosialin is roughly equivalent to that on cell surfaces of HL-60 cells. 3) Leukosialin from K562 cells, on the other hand, contains no detectable amount of poly-N-acetyllactosaminyl O-glycans. 4) The presence of poly-N-acetyllactosamine in O-glycans is dependent on the core 2 beta 1,6-N-acetylglucosaminyl transferase. 5) Jacalin-agarose binds to sialylated small oligosaccharides such as NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e6) GalNAc but not the hexasaccharide NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4GlcNAc beta 1--\u003e6) GalNAc. These results indicate that the formation of polylactosaminyl O-glycans and sialyl Le(x) structure in O-glycans is dependent on the core 2 formation."}

GlyCosmos15-MAT

{"project":"GlyCosmos15-MAT","denotations":[{"id":"T1","span":{"begin":1357,"end":1361},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000091"}],"text":"Poly-N-acetyllactosaminyl O-glycans attached to leukosialin. The presence of sialyl Le(x) structures in O-glycans.\nPoly-N-acetyllactosamine extension has been found in O-glycans in addition to N-glycans and glycosphingolipids. Attempts were made in HL-60 and K562 cells to determine the amount of poly-N-acetyllactosaminyl O-glycans in the major sialoglycoprotein, leukosialin. Leukosialin was immunoprecipitated from [3H]glucosamine-labeled HL-60 and K562 cells. Glycopeptides were prepared by Pronase digestion, and O-glycan-containing glycopeptides were isolated by affinity chromatography using Jacalin-agarose. The glycopeptides bound to Jacalin-agarose and those unbound were treated with alkaline borohydride, and the released O-glycans were fractionated by Bio-Gel P-4 filtration. Sequential glycosidase digestion of the O-glycans, with or without pretreatment by fucosidase or neuraminidase, revealed the following conclusions. 1) Leukosialin from HL-60 cells contains about 1-2 poly-N-acetyllactosaminyl O-glycan chains/molecule. 2) About 50% of these poly-N-acetyllactosaminyl O-glycans contain sialyl Le(x) termini, NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 (Fuc alpha 1--\u003e3)GlcNAc beta 1--\u003eR. The amount of sialyl Le(x) structure in leukosialin is roughly equivalent to that on cell surfaces of HL-60 cells. 3) Leukosialin from K562 cells, on the other hand, contains no detectable amount of poly-N-acetyllactosaminyl O-glycans. 4) The presence of poly-N-acetyllactosamine in O-glycans is dependent on the core 2 beta 1,6-N-acetylglucosaminyl transferase. 5) Jacalin-agarose binds to sialylated small oligosaccharides such as NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e6) GalNAc but not the hexasaccharide NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4GlcNAc beta 1--\u003e6) GalNAc. These results indicate that the formation of polylactosaminyl O-glycans and sialyl Le(x) structure in O-glycans is dependent on the core 2 formation."}

sentences

GlyCosmos15-Sentences

{"project":"GlyCosmos15-Sentences","blocks":[{"id":"T1","span":{"begin":0,"end":60},"obj":"Sentence"},{"id":"T2","span":{"begin":61,"end":114},"obj":"Sentence"},{"id":"T3","span":{"begin":115,"end":226},"obj":"Sentence"},{"id":"T4","span":{"begin":227,"end":377},"obj":"Sentence"},{"id":"T5","span":{"begin":378,"end":463},"obj":"Sentence"},{"id":"T6","span":{"begin":464,"end":615},"obj":"Sentence"},{"id":"T7","span":{"begin":616,"end":788},"obj":"Sentence"},{"id":"T8","span":{"begin":789,"end":936},"obj":"Sentence"},{"id":"T9","span":{"begin":937,"end":1039},"obj":"Sentence"},{"id":"T10","span":{"begin":1040,"end":1311},"obj":"Sentence"},{"id":"T11","span":{"begin":1312,"end":1432},"obj":"Sentence"},{"id":"T12","span":{"begin":1433,"end":1559},"obj":"Sentence"},{"id":"T13","span":{"begin":1560,"end":1808},"obj":"Sentence"},{"id":"T14","span":{"begin":1809,"end":1958},"obj":"Sentence"}],"text":"Poly-N-acetyllactosaminyl O-glycans attached to leukosialin. The presence of sialyl Le(x) structures in O-glycans.\nPoly-N-acetyllactosamine extension has been found in O-glycans in addition to N-glycans and glycosphingolipids. Attempts were made in HL-60 and K562 cells to determine the amount of poly-N-acetyllactosaminyl O-glycans in the major sialoglycoprotein, leukosialin. Leukosialin was immunoprecipitated from [3H]glucosamine-labeled HL-60 and K562 cells. Glycopeptides were prepared by Pronase digestion, and O-glycan-containing glycopeptides were isolated by affinity chromatography using Jacalin-agarose. The glycopeptides bound to Jacalin-agarose and those unbound were treated with alkaline borohydride, and the released O-glycans were fractionated by Bio-Gel P-4 filtration. Sequential glycosidase digestion of the O-glycans, with or without pretreatment by fucosidase or neuraminidase, revealed the following conclusions. 1) Leukosialin from HL-60 cells contains about 1-2 poly-N-acetyllactosaminyl O-glycan chains/molecule. 2) About 50% of these poly-N-acetyllactosaminyl O-glycans contain sialyl Le(x) termini, NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 (Fuc alpha 1--\u003e3)GlcNAc beta 1--\u003eR. The amount of sialyl Le(x) structure in leukosialin is roughly equivalent to that on cell surfaces of HL-60 cells. 3) Leukosialin from K562 cells, on the other hand, contains no detectable amount of poly-N-acetyllactosaminyl O-glycans. 4) The presence of poly-N-acetyllactosamine in O-glycans is dependent on the core 2 beta 1,6-N-acetylglucosaminyl transferase. 5) Jacalin-agarose binds to sialylated small oligosaccharides such as NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e6) GalNAc but not the hexasaccharide NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4GlcNAc beta 1--\u003e6) GalNAc. These results indicate that the formation of polylactosaminyl O-glycans and sialyl Le(x) structure in O-glycans is dependent on the core 2 formation."}

GlyCosmos15-Glycan

Anatomy-UBERON

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":140,"end":149},"obj":"Body_part"},{"id":"T2","span":{"begin":1357,"end":1361},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_2000106"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0002398"}],"text":"Poly-N-acetyllactosaminyl O-glycans attached to leukosialin. The presence of sialyl Le(x) structures in O-glycans.\nPoly-N-acetyllactosamine extension has been found in O-glycans in addition to N-glycans and glycosphingolipids. Attempts were made in HL-60 and K562 cells to determine the amount of poly-N-acetyllactosaminyl O-glycans in the major sialoglycoprotein, leukosialin. Leukosialin was immunoprecipitated from [3H]glucosamine-labeled HL-60 and K562 cells. Glycopeptides were prepared by Pronase digestion, and O-glycan-containing glycopeptides were isolated by affinity chromatography using Jacalin-agarose. The glycopeptides bound to Jacalin-agarose and those unbound were treated with alkaline borohydride, and the released O-glycans were fractionated by Bio-Gel P-4 filtration. Sequential glycosidase digestion of the O-glycans, with or without pretreatment by fucosidase or neuraminidase, revealed the following conclusions. 1) Leukosialin from HL-60 cells contains about 1-2 poly-N-acetyllactosaminyl O-glycan chains/molecule. 2) About 50% of these poly-N-acetyllactosaminyl O-glycans contain sialyl Le(x) termini, NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 (Fuc alpha 1--\u003e3)GlcNAc beta 1--\u003eR. The amount of sialyl Le(x) structure in leukosialin is roughly equivalent to that on cell surfaces of HL-60 cells. 3) Leukosialin from K562 cells, on the other hand, contains no detectable amount of poly-N-acetyllactosaminyl O-glycans. 4) The presence of poly-N-acetyllactosamine in O-glycans is dependent on the core 2 beta 1,6-N-acetylglucosaminyl transferase. 5) Jacalin-agarose binds to sialylated small oligosaccharides such as NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e6) GalNAc but not the hexasaccharide NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4GlcNAc beta 1--\u003e6) GalNAc. These results indicate that the formation of polylactosaminyl O-glycans and sialyl Le(x) structure in O-glycans is dependent on the core 2 formation."}

CL-cell

{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":0,"end":4},"obj":"Cell"},{"id":"T3","span":{"begin":115,"end":119},"obj":"Cell"},{"id":"T5","span":{"begin":297,"end":301},"obj":"Cell"},{"id":"T7","span":{"begin":988,"end":992},"obj":"Cell"},{"id":"T9","span":{"begin":1062,"end":1066},"obj":"Cell"},{"id":"T11","span":{"begin":1396,"end":1400},"obj":"Cell"},{"id":"T13","span":{"begin":1452,"end":1456},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000096"},{"id":"A2","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000775"},{"id":"A3","pred":"cl_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL:0000096"},{"id":"A4","pred":"cl_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL:0000775"},{"id":"A5","pred":"cl_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CL:0000096"},{"id":"A6","pred":"cl_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CL:0000775"},{"id":"A7","pred":"cl_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/CL:0000096"},{"id":"A8","pred":"cl_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/CL:0000775"},{"id":"A9","pred":"cl_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/CL:0000096"},{"id":"A10","pred":"cl_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/CL:0000775"},{"id":"A11","pred":"cl_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/CL:0000096"},{"id":"A12","pred":"cl_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/CL:0000775"},{"id":"A13","pred":"cl_id","subj":"T13","obj":"http://purl.obolibrary.org/obo/CL:0000096"},{"id":"A14","pred":"cl_id","subj":"T13","obj":"http://purl.obolibrary.org/obo/CL:0000775"}],"text":"Poly-N-acetyllactosaminyl O-glycans attached to leukosialin. The presence of sialyl Le(x) structures in O-glycans.\nPoly-N-acetyllactosamine extension has been found in O-glycans in addition to N-glycans and glycosphingolipids. Attempts were made in HL-60 and K562 cells to determine the amount of poly-N-acetyllactosaminyl O-glycans in the major sialoglycoprotein, leukosialin. Leukosialin was immunoprecipitated from [3H]glucosamine-labeled HL-60 and K562 cells. Glycopeptides were prepared by Pronase digestion, and O-glycan-containing glycopeptides were isolated by affinity chromatography using Jacalin-agarose. The glycopeptides bound to Jacalin-agarose and those unbound were treated with alkaline borohydride, and the released O-glycans were fractionated by Bio-Gel P-4 filtration. Sequential glycosidase digestion of the O-glycans, with or without pretreatment by fucosidase or neuraminidase, revealed the following conclusions. 1) Leukosialin from HL-60 cells contains about 1-2 poly-N-acetyllactosaminyl O-glycan chains/molecule. 2) About 50% of these poly-N-acetyllactosaminyl O-glycans contain sialyl Le(x) termini, NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 (Fuc alpha 1--\u003e3)GlcNAc beta 1--\u003eR. The amount of sialyl Le(x) structure in leukosialin is roughly equivalent to that on cell surfaces of HL-60 cells. 3) Leukosialin from K562 cells, on the other hand, contains no detectable amount of poly-N-acetyllactosaminyl O-glycans. 4) The presence of poly-N-acetyllactosamine in O-glycans is dependent on the core 2 beta 1,6-N-acetylglucosaminyl transferase. 5) Jacalin-agarose binds to sialylated small oligosaccharides such as NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e6) GalNAc but not the hexasaccharide NeuNAc alpha 2--\u003e3Gal beta 1--\u003e3(NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4GlcNAc beta 1--\u003e6) GalNAc. These results indicate that the formation of polylactosaminyl O-glycans and sialyl Le(x) structure in O-glycans is dependent on the core 2 formation."}