PubMed:1429562 JSONTXT

{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":112},"obj":"Sentence"},{"id":"T2","span":{"begin":113,"end":400},"obj":"Sentence"},{"id":"T3","span":{"begin":401,"end":518},"obj":"Sentence"},{"id":"T4","span":{"begin":519,"end":690},"obj":"Sentence"},{"id":"T5","span":{"begin":691,"end":806},"obj":"Sentence"},{"id":"T6","span":{"begin":807,"end":885},"obj":"Sentence"},{"id":"T7","span":{"begin":886,"end":960},"obj":"Sentence"},{"id":"T8","span":{"begin":961,"end":1055},"obj":"Sentence"},{"id":"T9","span":{"begin":1056,"end":1162},"obj":"Sentence"},{"id":"T10","span":{"begin":1163,"end":1320},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":112},"obj":"Sentence"},{"id":"T2","span":{"begin":113,"end":400},"obj":"Sentence"},{"id":"T3","span":{"begin":401,"end":518},"obj":"Sentence"},{"id":"T4","span":{"begin":519,"end":690},"obj":"Sentence"},{"id":"T5","span":{"begin":691,"end":806},"obj":"Sentence"},{"id":"T6","span":{"begin":807,"end":885},"obj":"Sentence"},{"id":"T7","span":{"begin":886,"end":960},"obj":"Sentence"},{"id":"T8","span":{"begin":961,"end":1055},"obj":"Sentence"},{"id":"T9","span":{"begin":1056,"end":1162},"obj":"Sentence"},{"id":"T10","span":{"begin":1163,"end":1320},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The regulation of the human tumor necrosis factor alpha promoter region in macrophage, T cell, and B cell lines.\nThe 1311-base pair human tumor necrosis factor (TNF) alpha promoter region was fused to the luciferase (Luc) reporter gene and studied in a transient transfection system in three TNF producing cell lines, the U937 macrophage cell line, the MLA 144 T cell line, and the 729-6 B cell line. This full length promoter construct can be induced by phorbol 13-myristate acetate (PMA) in each of these cell types. Analysis of a series of 5'-truncations showed several peaks of basal and PMA induced activity suggesting the presence of several positive and negative regulatory elements. A PMA responsive element was localized to a region between -95 and -36 bp relative to the transcription start site. Within this region, single AP-2- and AP-1-like consensus sequences were noted. These AP-2 and AP-1 sites were each modified with a double point mutation. A modest (20-50%) reduction in TNF promoter activity was observed with the AP-2 site mutation. However, mutation of the AP-1 site markedly diminished both the basal and PMA-activated promoter activity. Also co-transfections of the wild-type promoter construct with an AP-1/c-jun expression vector resulted in augmented basal and PMA-induced promoter activity."}

{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":138,"end":143},"obj":"HP_0002664"}],"text":"The regulation of the human tumor necrosis factor alpha promoter region in macrophage, T cell, and B cell lines.\nThe 1311-base pair human tumor necrosis factor (TNF) alpha promoter region was fused to the luciferase (Luc) reporter gene and studied in a transient transfection system in three TNF producing cell lines, the U937 macrophage cell line, the MLA 144 T cell line, and the 729-6 B cell line. This full length promoter construct can be induced by phorbol 13-myristate acetate (PMA) in each of these cell types. Analysis of a series of 5'-truncations showed several peaks of basal and PMA induced activity suggesting the presence of several positive and negative regulatory elements. A PMA responsive element was localized to a region between -95 and -36 bp relative to the transcription start site. Within this region, single AP-2- and AP-1-like consensus sequences were noted. These AP-2 and AP-1 sites were each modified with a double point mutation. A modest (20-50%) reduction in TNF promoter activity was observed with the AP-2 site mutation. However, mutation of the AP-1 site markedly diminished both the basal and PMA-activated promoter activity. Also co-transfections of the wild-type promoter construct with an AP-1/c-jun expression vector resulted in augmented basal and PMA-induced promoter activity."}

{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":22,"end":71},"obj":"DNA"},{"id":"T2","span":{"begin":75,"end":85},"obj":"cell_line"},{"id":"T3","span":{"begin":87,"end":93},"obj":"cell_line"},{"id":"T4","span":{"begin":99,"end":111},"obj":"cell_line"},{"id":"T5","span":{"begin":117,"end":187},"obj":"DNA"},{"id":"T6","span":{"begin":205,"end":235},"obj":"DNA"},{"id":"T7","span":{"begin":292,"end":316},"obj":"cell_line"},{"id":"T8","span":{"begin":322,"end":347},"obj":"cell_line"},{"id":"T9","span":{"begin":353,"end":372},"obj":"cell_line"},{"id":"T10","span":{"begin":382,"end":399},"obj":"cell_line"},{"id":"T11","span":{"begin":543,"end":557},"obj":"DNA"},{"id":"T12","span":{"begin":648,"end":656},"obj":"DNA"},{"id":"T13","span":{"begin":693,"end":715},"obj":"DNA"},{"id":"T14","span":{"begin":750,"end":764},"obj":"DNA"},{"id":"T15","span":{"begin":781,"end":805},"obj":"DNA"},{"id":"T16","span":{"begin":834,"end":873},"obj":"DNA"},{"id":"T17","span":{"begin":892,"end":896},"obj":"DNA"},{"id":"T18","span":{"begin":901,"end":911},"obj":"DNA"},{"id":"T19","span":{"begin":992,"end":1004},"obj":"DNA"},{"id":"T20","span":{"begin":1036,"end":1054},"obj":"DNA"},{"id":"T21","span":{"begin":1081,"end":1090},"obj":"DNA"},{"id":"T22","span":{"begin":1192,"end":1220},"obj":"DNA"},{"id":"T23","span":{"begin":1229,"end":1257},"obj":"DNA"}],"text":"The regulation of the human tumor necrosis factor alpha promoter region in macrophage, T cell, and B cell lines.\nThe 1311-base pair human tumor necrosis factor (TNF) alpha promoter region was fused to the luciferase (Luc) reporter gene and studied in a transient transfection system in three TNF producing cell lines, the U937 macrophage cell line, the MLA 144 T cell line, and the 729-6 B cell line. This full length promoter construct can be induced by phorbol 13-myristate acetate (PMA) in each of these cell types. Analysis of a series of 5'-truncations showed several peaks of basal and PMA induced activity suggesting the presence of several positive and negative regulatory elements. A PMA responsive element was localized to a region between -95 and -36 bp relative to the transcription start site. Within this region, single AP-2- and AP-1-like consensus sequences were noted. These AP-2 and AP-1 sites were each modified with a double point mutation. A modest (20-50%) reduction in TNF promoter activity was observed with the AP-2 site mutation. However, mutation of the AP-1 site markedly diminished both the basal and PMA-activated promoter activity. Also co-transfections of the wild-type promoter construct with an AP-1/c-jun expression vector resulted in augmented basal and PMA-induced promoter activity."}

{"project":"genia-medco-coref","denotations":[{"id":"C2","span":{"begin":28,"end":49},"obj":"NP"},{"id":"C1","span":{"begin":18,"end":71},"obj":"NP"},{"id":"C3","span":{"begin":75,"end":111},"obj":"NP"},{"id":"C5","span":{"begin":138,"end":165},"obj":"NP"},{"id":"C4","span":{"begin":113,"end":187},"obj":"NP"},{"id":"C7","span":{"begin":292,"end":295},"obj":"NP"},{"id":"C6","span":{"begin":286,"end":316},"obj":"NP"},{"id":"C8","span":{"begin":318,"end":399},"obj":"NP"},{"id":"C9","span":{"begin":401,"end":436},"obj":"NP"},{"id":"C10","span":{"begin":455,"end":489},"obj":"NP"},{"id":"C11","span":{"begin":501,"end":517},"obj":"NP"},{"id":"C13","span":{"begin":592,"end":595},"obj":"NP"},{"id":"C12","span":{"begin":582,"end":612},"obj":"NP"},{"id":"C14","span":{"begin":693,"end":696},"obj":"NP"},{"id":"C15","span":{"begin":733,"end":805},"obj":"NP"},{"id":"C16","span":{"begin":814,"end":825},"obj":"NP"},{"id":"C17","span":{"begin":827,"end":873},"obj":"NP"},{"id":"C18","span":{"begin":886,"end":911},"obj":"NP"},{"id":"C19","span":{"begin":992,"end":995},"obj":"NP"},{"id":"C20","span":{"begin":1111,"end":1161},"obj":"NP"},{"id":"C21","span":{"begin":1270,"end":1319},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C5","obj":"C2"},{"id":"R2","pred":"coref-ident","subj":"C4","obj":"C1"},{"id":"R3","pred":"coref-ident","subj":"C7","obj":"C5"},{"id":"R4","pred":"coref-ident","subj":"C6","obj":"C3"},{"id":"R5","pred":"coref-appos","subj":"C8","obj":"C6"},{"id":"R6","pred":"coref-ident","subj":"C9","obj":"C4"},{"id":"R7","pred":"coref-other","subj":"C11","obj":"C6"},{"id":"R8","pred":"coref-ident","subj":"C13","obj":"C10"},{"id":"R9","pred":"coref-ident","subj":"C14","obj":"C13"},{"id":"R10","pred":"coref-ident","subj":"C16","obj":"C15"},{"id":"R11","pred":"coref-ident","subj":"C18","obj":"C17"},{"id":"R12","pred":"coref-ident","subj":"C19","obj":"C7"},{"id":"R13","pred":"coref-ident","subj":"C20","obj":"C12"},{"id":"R14","pred":"coref-other","subj":"C21","obj":"C20"}],"text":"The regulation of the human tumor necrosis factor alpha promoter region in macrophage, T cell, and B cell lines.\nThe 1311-base pair human tumor necrosis factor (TNF) alpha promoter region was fused to the luciferase (Luc) reporter gene and studied in a transient transfection system in three TNF producing cell lines, the U937 macrophage cell line, the MLA 144 T cell line, and the 729-6 B cell line. This full length promoter construct can be induced by phorbol 13-myristate acetate (PMA) in each of these cell types. Analysis of a series of 5'-truncations showed several peaks of basal and PMA induced activity suggesting the presence of several positive and negative regulatory elements. A PMA responsive element was localized to a region between -95 and -36 bp relative to the transcription start site. Within this region, single AP-2- and AP-1-like consensus sequences were noted. These AP-2 and AP-1 sites were each modified with a double point mutation. A modest (20-50%) reduction in TNF promoter activity was observed with the AP-2 site mutation. However, mutation of the AP-1 site markedly diminished both the basal and PMA-activated promoter activity. Also co-transfections of the wild-type promoter construct with an AP-1/c-jun expression vector resulted in augmented basal and PMA-induced promoter activity."}

{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":112},"obj":"Sentence"},{"id":"S2","span":{"begin":113,"end":400},"obj":"Sentence"},{"id":"S3","span":{"begin":401,"end":518},"obj":"Sentence"},{"id":"S4","span":{"begin":519,"end":690},"obj":"Sentence"},{"id":"S5","span":{"begin":691,"end":806},"obj":"Sentence"},{"id":"S6","span":{"begin":807,"end":885},"obj":"Sentence"},{"id":"S7","span":{"begin":886,"end":960},"obj":"Sentence"},{"id":"S8","span":{"begin":961,"end":1055},"obj":"Sentence"},{"id":"S9","span":{"begin":1056,"end":1162},"obj":"Sentence"},{"id":"S10","span":{"begin":1163,"end":1320},"obj":"Sentence"}],"text":"The regulation of the human tumor necrosis factor alpha promoter region in macrophage, T cell, and B cell lines.\nThe 1311-base pair human tumor necrosis factor (TNF) alpha promoter region was fused to the luciferase (Luc) reporter gene and studied in a transient transfection system in three TNF producing cell lines, the U937 macrophage cell line, the MLA 144 T cell line, and the 729-6 B cell line. This full length promoter construct can be induced by phorbol 13-myristate acetate (PMA) in each of these cell types. Analysis of a series of 5'-truncations showed several peaks of basal and PMA induced activity suggesting the presence of several positive and negative regulatory elements. A PMA responsive element was localized to a region between -95 and -36 bp relative to the transcription start site. Within this region, single AP-2- and AP-1-like consensus sequences were noted. These AP-2 and AP-1 sites were each modified with a double point mutation. A modest (20-50%) reduction in TNF promoter activity was observed with the AP-2 site mutation. However, mutation of the AP-1 site markedly diminished both the basal and PMA-activated promoter activity. Also co-transfections of the wild-type promoter construct with an AP-1/c-jun expression vector resulted in augmented basal and PMA-induced promoter activity."}

{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":22,"end":71},"obj":"DNA_domain_or_region"},{"id":"T2","span":{"begin":75,"end":85},"obj":"cell_line"},{"id":"T3","span":{"begin":87,"end":93},"obj":"cell_line"},{"id":"T4","span":{"begin":99,"end":111},"obj":"cell_line"},{"id":"T5","span":{"begin":117,"end":187},"obj":"DNA_domain_or_region"},{"id":"T6","span":{"begin":205,"end":235},"obj":"DNA_molecule"},{"id":"T7","span":{"begin":253,"end":282},"obj":"other_name"},{"id":"T8","span":{"begin":292,"end":316},"obj":"cell_line"},{"id":"T9","span":{"begin":322,"end":347},"obj":"cell_line"},{"id":"T10","span":{"begin":353,"end":372},"obj":"cell_line"},{"id":"T11","span":{"begin":382,"end":399},"obj":"cell_line"},{"id":"T12","span":{"begin":455,"end":483},"obj":"other_organic_compound"},{"id":"T13","span":{"begin":485,"end":488},"obj":"other_organic_compound"},{"id":"T14","span":{"begin":543,"end":557},"obj":"DNA_molecule"},{"id":"T15","span":{"begin":648,"end":656},"obj":"DNA_domain_or_region"},{"id":"T16","span":{"begin":693,"end":696},"obj":"other_organic_compound"},{"id":"T17","span":{"begin":750,"end":764},"obj":"DNA_domain_or_region"},{"id":"T18","span":{"begin":781,"end":805},"obj":"DNA_domain_or_region"},{"id":"T19","span":{"begin":892,"end":896},"obj":"DNA_domain_or_region"},{"id":"T20","span":{"begin":901,"end":911},"obj":"DNA_domain_or_region"},{"id":"T21","span":{"begin":938,"end":959},"obj":"other_name"},{"id":"T22","span":{"begin":992,"end":1004},"obj":"DNA_domain_or_region"},{"id":"T23","span":{"begin":1036,"end":1054},"obj":"DNA_domain_or_region"},{"id":"T24","span":{"begin":1065,"end":1073},"obj":"other_name"},{"id":"T25","span":{"begin":1081,"end":1090},"obj":"DNA_domain_or_region"},{"id":"T26","span":{"begin":1130,"end":1161},"obj":"other_name"},{"id":"T27","span":{"begin":1192,"end":1220},"obj":"DNA_molecule"},{"id":"T28","span":{"begin":1229,"end":1257},"obj":"DNA_molecule"},{"id":"T29","span":{"begin":1290,"end":1293},"obj":"other_organic_compound"}],"text":"The regulation of the human tumor necrosis factor alpha promoter region in macrophage, T cell, and B cell lines.\nThe 1311-base pair human tumor necrosis factor (TNF) alpha promoter region was fused to the luciferase (Luc) reporter gene and studied in a transient transfection system in three TNF producing cell lines, the U937 macrophage cell line, the MLA 144 T cell line, and the 729-6 B cell line. This full length promoter construct can be induced by phorbol 13-myristate acetate (PMA) in each of these cell types. Analysis of a series of 5'-truncations showed several peaks of basal and PMA induced activity suggesting the presence of several positive and negative regulatory elements. A PMA responsive element was localized to a region between -95 and -36 bp relative to the transcription start site. Within this region, single AP-2- and AP-1-like consensus sequences were noted. These AP-2 and AP-1 sites were each modified with a double point mutation. A modest (20-50%) reduction in TNF promoter activity was observed with the AP-2 site mutation. However, mutation of the AP-1 site markedly diminished both the basal and PMA-activated promoter activity. Also co-transfections of the wild-type promoter construct with an AP-1/c-jun expression vector resulted in augmented basal and PMA-induced promoter activity."}

{"project":"HP-phenotype","denotations":[{"id":"T1","span":{"begin":28,"end":33},"obj":"Phenotype"},{"id":"T2","span":{"begin":138,"end":143},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0002664"},{"id":"A2","pred":"hp_id","subj":"T2","obj":"HP:0002664"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}],"text":"The regulation of the human tumor necrosis factor alpha promoter region in macrophage, T cell, and B cell lines.\nThe 1311-base pair human tumor necrosis factor (TNF) alpha promoter region was fused to the luciferase (Luc) reporter gene and studied in a transient transfection system in three TNF producing cell lines, the U937 macrophage cell line, the MLA 144 T cell line, and the 729-6 B cell line. This full length promoter construct can be induced by phorbol 13-myristate acetate (PMA) in each of these cell types. Analysis of a series of 5'-truncations showed several peaks of basal and PMA induced activity suggesting the presence of several positive and negative regulatory elements. A PMA responsive element was localized to a region between -95 and -36 bp relative to the transcription start site. Within this region, single AP-2- and AP-1-like consensus sequences were noted. These AP-2 and AP-1 sites were each modified with a double point mutation. A modest (20-50%) reduction in TNF promoter activity was observed with the AP-2 site mutation. However, mutation of the AP-1 site markedly diminished both the basal and PMA-activated promoter activity. Also co-transfections of the wild-type promoter construct with an AP-1/c-jun expression vector resulted in augmented basal and PMA-induced promoter activity."}

{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":28,"end":33},"obj":"Disease"},{"id":"T2","span":{"begin":138,"end":143},"obj":"Disease"},{"id":"T3","span":{"begin":485,"end":488},"obj":"Disease"},{"id":"T5","span":{"begin":592,"end":595},"obj":"Disease"},{"id":"T7","span":{"begin":693,"end":696},"obj":"Disease"},{"id":"T9","span":{"begin":1130,"end":1133},"obj":"Disease"},{"id":"T11","span":{"begin":1290,"end":1293},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0005070"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0005070"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0016692"},{"id":"A4","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0018687"},{"id":"A5","pred":"mondo_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MONDO_0016692"},{"id":"A6","pred":"mondo_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MONDO_0018687"},{"id":"A7","pred":"mondo_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/MONDO_0016692"},{"id":"A8","pred":"mondo_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/MONDO_0018687"},{"id":"A9","pred":"mondo_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/MONDO_0016692"},{"id":"A10","pred":"mondo_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/MONDO_0018687"},{"id":"A11","pred":"mondo_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/MONDO_0016692"},{"id":"A12","pred":"mondo_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/MONDO_0018687"}],"text":"The regulation of the human tumor necrosis factor alpha promoter region in macrophage, T cell, and B cell lines.\nThe 1311-base pair human tumor necrosis factor (TNF) alpha promoter region was fused to the luciferase (Luc) reporter gene and studied in a transient transfection system in three TNF producing cell lines, the U937 macrophage cell line, the MLA 144 T cell line, and the 729-6 B cell line. This full length promoter construct can be induced by phorbol 13-myristate acetate (PMA) in each of these cell types. Analysis of a series of 5'-truncations showed several peaks of basal and PMA induced activity suggesting the presence of several positive and negative regulatory elements. A PMA responsive element was localized to a region between -95 and -36 bp relative to the transcription start site. Within this region, single AP-2- and AP-1-like consensus sequences were noted. These AP-2 and AP-1 sites were each modified with a double point mutation. A modest (20-50%) reduction in TNF promoter activity was observed with the AP-2 site mutation. However, mutation of the AP-1 site markedly diminished both the basal and PMA-activated promoter activity. Also co-transfections of the wild-type promoter construct with an AP-1/c-jun expression vector resulted in augmented basal and PMA-induced promoter activity."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":22,"end":27},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":132,"end":137},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9606"}],"text":"The regulation of the human tumor necrosis factor alpha promoter region in macrophage, T cell, and B cell lines.\nThe 1311-base pair human tumor necrosis factor (TNF) alpha promoter region was fused to the luciferase (Luc) reporter gene and studied in a transient transfection system in three TNF producing cell lines, the U937 macrophage cell line, the MLA 144 T cell line, and the 729-6 B cell line. This full length promoter construct can be induced by phorbol 13-myristate acetate (PMA) in each of these cell types. Analysis of a series of 5'-truncations showed several peaks of basal and PMA induced activity suggesting the presence of several positive and negative regulatory elements. A PMA responsive element was localized to a region between -95 and -36 bp relative to the transcription start site. Within this region, single AP-2- and AP-1-like consensus sequences were noted. These AP-2 and AP-1 sites were each modified with a double point mutation. A modest (20-50%) reduction in TNF promoter activity was observed with the AP-2 site mutation. However, mutation of the AP-1 site markedly diminished both the basal and PMA-activated promoter activity. Also co-transfections of the wild-type promoter construct with an AP-1/c-jun expression vector resulted in augmented basal and PMA-induced promoter activity."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":75,"end":85},"obj":"Body_part"},{"id":"T2","span":{"begin":87,"end":93},"obj":"Body_part"},{"id":"T3","span":{"begin":99,"end":105},"obj":"Body_part"},{"id":"T4","span":{"begin":327,"end":337},"obj":"Body_part"},{"id":"T5","span":{"begin":361,"end":367},"obj":"Body_part"},{"id":"T6","span":{"begin":388,"end":394},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL_0000235"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL_0000084"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL_0000236"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL_0000235"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CL_0000084"},{"id":"A6","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/CL_0000236"}],"text":"The regulation of the human tumor necrosis factor alpha promoter region in macrophage, T cell, and B cell lines.\nThe 1311-base pair human tumor necrosis factor (TNF) alpha promoter region was fused to the luciferase (Luc) reporter gene and studied in a transient transfection system in three TNF producing cell lines, the U937 macrophage cell line, the MLA 144 T cell line, and the 729-6 B cell line. This full length promoter construct can be induced by phorbol 13-myristate acetate (PMA) in each of these cell types. Analysis of a series of 5'-truncations showed several peaks of basal and PMA induced activity suggesting the presence of several positive and negative regulatory elements. A PMA responsive element was localized to a region between -95 and -36 bp relative to the transcription start site. Within this region, single AP-2- and AP-1-like consensus sequences were noted. These AP-2 and AP-1 sites were each modified with a double point mutation. A modest (20-50%) reduction in TNF promoter activity was observed with the AP-2 site mutation. However, mutation of the AP-1 site markedly diminished both the basal and PMA-activated promoter activity. Also co-transfections of the wild-type promoter construct with an AP-1/c-jun expression vector resulted in augmented basal and PMA-induced promoter activity."}

{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":75,"end":85},"obj":"Cell"},{"id":"T3","span":{"begin":87,"end":93},"obj":"Cell"},{"id":"T4","span":{"begin":99,"end":105},"obj":"Cell"},{"id":"T5","span":{"begin":327,"end":337},"obj":"Cell"},{"id":"T7","span":{"begin":361,"end":367},"obj":"Cell"},{"id":"T8","span":{"begin":388,"end":394},"obj":"Cell"},{"id":"T9","span":{"begin":582,"end":587},"obj":"Cell"},{"id":"T10","span":{"begin":1120,"end":1125},"obj":"Cell"},{"id":"T11","span":{"begin":1280,"end":1285},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000235"},{"id":"A2","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000394"},{"id":"A3","pred":"cl_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A4","pred":"cl_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL:0000236"},{"id":"A5","pred":"cl_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CL:0000235"},{"id":"A6","pred":"cl_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CL:0000394"},{"id":"A7","pred":"cl_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A8","pred":"cl_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/CL:0000236"},{"id":"A9","pred":"cl_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/CL:0002324"},{"id":"A10","pred":"cl_id","subj":"T10","obj":"http://purl.obolibrary.org/obo/CL:0002324"},{"id":"A11","pred":"cl_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/CL:0002324"}],"text":"The regulation of the human tumor necrosis factor alpha promoter region in macrophage, T cell, and B cell lines.\nThe 1311-base pair human tumor necrosis factor (TNF) alpha promoter region was fused to the luciferase (Luc) reporter gene and studied in a transient transfection system in three TNF producing cell lines, the U937 macrophage cell line, the MLA 144 T cell line, and the 729-6 B cell line. This full length promoter construct can be induced by phorbol 13-myristate acetate (PMA) in each of these cell types. Analysis of a series of 5'-truncations showed several peaks of basal and PMA induced activity suggesting the presence of several positive and negative regulatory elements. A PMA responsive element was localized to a region between -95 and -36 bp relative to the transcription start site. Within this region, single AP-2- and AP-1-like consensus sequences were noted. These AP-2 and AP-1 sites were each modified with a double point mutation. A modest (20-50%) reduction in TNF promoter activity was observed with the AP-2 site mutation. However, mutation of the AP-1 site markedly diminished both the basal and PMA-activated promoter activity. Also co-transfections of the wild-type promoter construct with an AP-1/c-jun expression vector resulted in augmented basal and PMA-induced promoter activity."}