PubMed:1339443 JSONTXT

{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":154,"end":161},"obj":"Glycan_Motif"},{"id":"T3","span":{"begin":163,"end":177},"obj":"Glycan_Motif"},{"id":"T4","span":{"begin":170,"end":177},"obj":"Glycan_Motif"},{"id":"T6","span":{"begin":193,"end":207},"obj":"Glycan_Motif"},{"id":"T7","span":{"begin":200,"end":207},"obj":"Glycan_Motif"},{"id":"T9","span":{"begin":616,"end":623},"obj":"Glycan_Motif"},{"id":"T11","span":{"begin":665,"end":679},"obj":"Glycan_Motif"},{"id":"T12","span":{"begin":672,"end":679},"obj":"Glycan_Motif"},{"id":"T14","span":{"begin":754,"end":768},"obj":"Glycan_Motif"},{"id":"T15","span":{"begin":761,"end":768},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G01187XC"},{"id":"A2","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00051MO"},{"id":"A3","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00054MO"},{"id":"A4","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G01187XC"},{"id":"A5","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00051MO"},{"id":"A6","pred":"image","subj":"T6","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00054MO"},{"id":"A7","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G01187XC"},{"id":"A8","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00051MO"},{"id":"A9","pred":"image","subj":"T9","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G01187XC"},{"id":"A10","pred":"image","subj":"T9","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00051MO"},{"id":"A11","pred":"image","subj":"T11","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00054MO"},{"id":"A12","pred":"image","subj":"T12","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G01187XC"},{"id":"A13","pred":"image","subj":"T12","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00051MO"},{"id":"A14","pred":"image","subj":"T14","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00054MO"},{"id":"A15","pred":"image","subj":"T15","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G01187XC"},{"id":"A16","pred":"image","subj":"T15","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00051MO"}],"text":"Molecular cloning of a fourth member of a human alpha (1,3)fucosyltransferase gene family. Multiple homologous sequences that determine expression of the Lewis x, sialyl Lewis x, and difucosyl sialyl Lewis x epitopes.\nWe and others have previously described the isolation of three human alpha (1,3)fucosyltransferase genes which form the basis of a nascent glycosyltransferase gene family. We now report the molecular cloning and expression of a fourth homologous human alpha (1,3)fucosyltransferase gene. When transfected into mammalian cells, this fucosyltransferase gene is capable of directing expression of the Lewis x (Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc), sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 [Fuc alpha 1--\u003e3]GlcNAc), and difucosyl sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc beta 1--\u003e3 Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc) epitopes. The enzyme shares 85% amino acid sequence identity with Fuc-TIII and 89% identity with Fuc-TV but differs substantially in its acceptor substrate requirements. Polymerase chain reaction analyses demonstrate that the gene is syntenic to Fuc-TIII and Fuc-TV on chromosome 19. Southern blot analyses of human genomic DNA demonstrate that these four alpha (1,3)fucosyltransferase genes account for all DNA sequences that cross-hybridize at low stringency with the Fuc-TIII catalytic domain. Using similar methods, a catalytic domain probe from Fuc-TIV identifies a new class of DNA fragments which do not cross-hybridize with the chromosome 19 fucosyltransferase probes. These results extend the molecular definition of a family of human alpha (1,3)fucosyltransferase genes and provide tools for examining fucosyltransferase gene expression."}

{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":90},"obj":"Sentence"},{"id":"T2","span":{"begin":91,"end":217},"obj":"Sentence"},{"id":"T3","span":{"begin":218,"end":389},"obj":"Sentence"},{"id":"T4","span":{"begin":390,"end":505},"obj":"Sentence"},{"id":"T5","span":{"begin":506,"end":885},"obj":"Sentence"},{"id":"T6","span":{"begin":886,"end":1045},"obj":"Sentence"},{"id":"T7","span":{"begin":1046,"end":1159},"obj":"Sentence"},{"id":"T8","span":{"begin":1160,"end":1372},"obj":"Sentence"},{"id":"T9","span":{"begin":1373,"end":1552},"obj":"Sentence"},{"id":"T10","span":{"begin":1553,"end":1723},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":90},"obj":"Sentence"},{"id":"T2","span":{"begin":91,"end":217},"obj":"Sentence"},{"id":"T3","span":{"begin":218,"end":389},"obj":"Sentence"},{"id":"T4","span":{"begin":390,"end":505},"obj":"Sentence"},{"id":"T5","span":{"begin":506,"end":885},"obj":"Sentence"},{"id":"T6","span":{"begin":886,"end":1045},"obj":"Sentence"},{"id":"T7","span":{"begin":1046,"end":1159},"obj":"Sentence"},{"id":"T8","span":{"begin":1160,"end":1372},"obj":"Sentence"},{"id":"T9","span":{"begin":1373,"end":1552},"obj":"Sentence"},{"id":"T10","span":{"begin":1553,"end":1723},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Molecular cloning of a fourth member of a human alpha (1,3)fucosyltransferase gene family. Multiple homologous sequences that determine expression of the Lewis x, sialyl Lewis x, and difucosyl sialyl Lewis x epitopes.\nWe and others have previously described the isolation of three human alpha (1,3)fucosyltransferase genes which form the basis of a nascent glycosyltransferase gene family. We now report the molecular cloning and expression of a fourth homologous human alpha (1,3)fucosyltransferase gene. When transfected into mammalian cells, this fucosyltransferase gene is capable of directing expression of the Lewis x (Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc), sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 [Fuc alpha 1--\u003e3]GlcNAc), and difucosyl sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc beta 1--\u003e3 Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc) epitopes. The enzyme shares 85% amino acid sequence identity with Fuc-TIII and 89% identity with Fuc-TV but differs substantially in its acceptor substrate requirements. Polymerase chain reaction analyses demonstrate that the gene is syntenic to Fuc-TIII and Fuc-TV on chromosome 19. Southern blot analyses of human genomic DNA demonstrate that these four alpha (1,3)fucosyltransferase genes account for all DNA sequences that cross-hybridize at low stringency with the Fuc-TIII catalytic domain. Using similar methods, a catalytic domain probe from Fuc-TIV identifies a new class of DNA fragments which do not cross-hybridize with the chromosome 19 fucosyltransferase probes. These results extend the molecular definition of a family of human alpha (1,3)fucosyltransferase genes and provide tools for examining fucosyltransferase gene expression."}

{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":154,"end":161},"obj":"https://glytoucan.org/Structures/Glycans/G00051MO"},{"id":"T2","span":{"begin":154,"end":161},"obj":"https://glytoucan.org/Structures/Glycans/G01187XC"},{"id":"T3","span":{"begin":163,"end":177},"obj":"https://glytoucan.org/Structures/Glycans/G00054MO"},{"id":"T4","span":{"begin":170,"end":177},"obj":"https://glytoucan.org/Structures/Glycans/G00051MO"},{"id":"T5","span":{"begin":170,"end":177},"obj":"https://glytoucan.org/Structures/Glycans/G01187XC"},{"id":"T6","span":{"begin":193,"end":207},"obj":"https://glytoucan.org/Structures/Glycans/G00054MO"},{"id":"T7","span":{"begin":200,"end":207},"obj":"https://glytoucan.org/Structures/Glycans/G00051MO"},{"id":"T8","span":{"begin":200,"end":207},"obj":"https://glytoucan.org/Structures/Glycans/G01187XC"},{"id":"T9","span":{"begin":616,"end":623},"obj":"https://glytoucan.org/Structures/Glycans/G00051MO"},{"id":"T10","span":{"begin":616,"end":623},"obj":"https://glytoucan.org/Structures/Glycans/G01187XC"},{"id":"T11","span":{"begin":665,"end":679},"obj":"https://glytoucan.org/Structures/Glycans/G00054MO"},{"id":"T12","span":{"begin":672,"end":679},"obj":"https://glytoucan.org/Structures/Glycans/G00051MO"},{"id":"T13","span":{"begin":672,"end":679},"obj":"https://glytoucan.org/Structures/Glycans/G01187XC"},{"id":"T14","span":{"begin":754,"end":768},"obj":"https://glytoucan.org/Structures/Glycans/G00054MO"},{"id":"T15","span":{"begin":761,"end":768},"obj":"https://glytoucan.org/Structures/Glycans/G00051MO"},{"id":"T16","span":{"begin":761,"end":768},"obj":"https://glytoucan.org/Structures/Glycans/G01187XC"}],"text":"Molecular cloning of a fourth member of a human alpha (1,3)fucosyltransferase gene family. Multiple homologous sequences that determine expression of the Lewis x, sialyl Lewis x, and difucosyl sialyl Lewis x epitopes.\nWe and others have previously described the isolation of three human alpha (1,3)fucosyltransferase genes which form the basis of a nascent glycosyltransferase gene family. We now report the molecular cloning and expression of a fourth homologous human alpha (1,3)fucosyltransferase gene. When transfected into mammalian cells, this fucosyltransferase gene is capable of directing expression of the Lewis x (Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc), sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 [Fuc alpha 1--\u003e3]GlcNAc), and difucosyl sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc beta 1--\u003e3 Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc) epitopes. The enzyme shares 85% amino acid sequence identity with Fuc-TIII and 89% identity with Fuc-TV but differs substantially in its acceptor substrate requirements. Polymerase chain reaction analyses demonstrate that the gene is syntenic to Fuc-TIII and Fuc-TV on chromosome 19. Southern blot analyses of human genomic DNA demonstrate that these four alpha (1,3)fucosyltransferase genes account for all DNA sequences that cross-hybridize at low stringency with the Fuc-TIII catalytic domain. Using similar methods, a catalytic domain probe from Fuc-TIV identifies a new class of DNA fragments which do not cross-hybridize with the chromosome 19 fucosyltransferase probes. These results extend the molecular definition of a family of human alpha (1,3)fucosyltransferase genes and provide tools for examining fucosyltransferase gene expression."}

{"project":"Glycosmos6-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":154,"end":161},"obj":"http://www.glycoepitope.jp/epitopes/EP0011"},{"id":"T2","span":{"begin":163,"end":177},"obj":"http://www.glycoepitope.jp/epitopes/EP0012"},{"id":"T3","span":{"begin":170,"end":177},"obj":"http://www.glycoepitope.jp/epitopes/EP0011"},{"id":"T4","span":{"begin":193,"end":207},"obj":"http://www.glycoepitope.jp/epitopes/EP0012"},{"id":"T5","span":{"begin":200,"end":207},"obj":"http://www.glycoepitope.jp/epitopes/EP0011"},{"id":"T6","span":{"begin":616,"end":623},"obj":"http://www.glycoepitope.jp/epitopes/EP0011"},{"id":"T7","span":{"begin":665,"end":679},"obj":"http://www.glycoepitope.jp/epitopes/EP0012"},{"id":"T8","span":{"begin":672,"end":679},"obj":"http://www.glycoepitope.jp/epitopes/EP0011"},{"id":"T9","span":{"begin":754,"end":768},"obj":"http://www.glycoepitope.jp/epitopes/EP0012"},{"id":"T10","span":{"begin":761,"end":768},"obj":"http://www.glycoepitope.jp/epitopes/EP0011"}],"text":"Molecular cloning of a fourth member of a human alpha (1,3)fucosyltransferase gene family. Multiple homologous sequences that determine expression of the Lewis x, sialyl Lewis x, and difucosyl sialyl Lewis x epitopes.\nWe and others have previously described the isolation of three human alpha (1,3)fucosyltransferase genes which form the basis of a nascent glycosyltransferase gene family. We now report the molecular cloning and expression of a fourth homologous human alpha (1,3)fucosyltransferase gene. When transfected into mammalian cells, this fucosyltransferase gene is capable of directing expression of the Lewis x (Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc), sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 [Fuc alpha 1--\u003e3]GlcNAc), and difucosyl sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc beta 1--\u003e3 Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc) epitopes. The enzyme shares 85% amino acid sequence identity with Fuc-TIII and 89% identity with Fuc-TV but differs substantially in its acceptor substrate requirements. Polymerase chain reaction analyses demonstrate that the gene is syntenic to Fuc-TIII and Fuc-TV on chromosome 19. Southern blot analyses of human genomic DNA demonstrate that these four alpha (1,3)fucosyltransferase genes account for all DNA sequences that cross-hybridize at low stringency with the Fuc-TIII catalytic domain. Using similar methods, a catalytic domain probe from Fuc-TIV identifies a new class of DNA fragments which do not cross-hybridize with the chromosome 19 fucosyltransferase probes. These results extend the molecular definition of a family of human alpha (1,3)fucosyltransferase genes and provide tools for examining fucosyltransferase gene expression."}

{"project":"NGLY1-deficiency","denotations":[{"id":"PD-NGLY1-deficiency-B_T1","span":{"begin":656,"end":662},"obj":"chem:24139"},{"id":"PD-NGLY1-deficiency-B_T2","span":{"begin":731,"end":737},"obj":"chem:24139"},{"id":"PD-NGLY1-deficiency-B_T3","span":{"begin":819,"end":825},"obj":"chem:24139"},{"id":"PD-NGLY1-deficiency-B_T4","span":{"begin":868,"end":874},"obj":"chem:24139"}],"namespaces":[{"prefix":"hgnc","uri":"https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:"},{"prefix":"omim","uri":"https://www.omim.org/entry/"},{"prefix":"chem","uri":"https://pubchem.ncbi.nlm.nih.gov/compound/"}],"text":"Molecular cloning of a fourth member of a human alpha (1,3)fucosyltransferase gene family. Multiple homologous sequences that determine expression of the Lewis x, sialyl Lewis x, and difucosyl sialyl Lewis x epitopes.\nWe and others have previously described the isolation of three human alpha (1,3)fucosyltransferase genes which form the basis of a nascent glycosyltransferase gene family. We now report the molecular cloning and expression of a fourth homologous human alpha (1,3)fucosyltransferase gene. When transfected into mammalian cells, this fucosyltransferase gene is capable of directing expression of the Lewis x (Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc), sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 [Fuc alpha 1--\u003e3]GlcNAc), and difucosyl sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc beta 1--\u003e3 Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc) epitopes. The enzyme shares 85% amino acid sequence identity with Fuc-TIII and 89% identity with Fuc-TV but differs substantially in its acceptor substrate requirements. Polymerase chain reaction analyses demonstrate that the gene is syntenic to Fuc-TIII and Fuc-TV on chromosome 19. Southern blot analyses of human genomic DNA demonstrate that these four alpha (1,3)fucosyltransferase genes account for all DNA sequences that cross-hybridize at low stringency with the Fuc-TIII catalytic domain. Using similar methods, a catalytic domain probe from Fuc-TIV identifies a new class of DNA fragments which do not cross-hybridize with the chromosome 19 fucosyltransferase probes. These results extend the molecular definition of a family of human alpha (1,3)fucosyltransferase genes and provide tools for examining fucosyltransferase gene expression."}

{"project":"Glycan-GlyCosmos","denotations":[{"id":"T1","span":{"begin":154,"end":161},"obj":"Glycan"},{"id":"T2","span":{"begin":163,"end":177},"obj":"Glycan"},{"id":"T3","span":{"begin":193,"end":207},"obj":"Glycan"},{"id":"T4","span":{"begin":616,"end":623},"obj":"Glycan"},{"id":"T5","span":{"begin":665,"end":679},"obj":"Glycan"},{"id":"T6","span":{"begin":681,"end":687},"obj":"Glycan"},{"id":"T7","span":{"begin":754,"end":768},"obj":"Glycan"},{"id":"T8","span":{"begin":770,"end":776},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G00051MO"},{"id":"A9","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00051MO"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G00054MO"},{"id":"A10","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G00054MO"},{"id":"A11","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO"},{"id":"A4","pred":"glycosmos_id","subj":"T4","obj":"https://glycosmos.org/glycans/show/G00051MO"},{"id":"A12","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00051MO"},{"id":"A5","pred":"glycosmos_id","subj":"T5","obj":"https://glycosmos.org/glycans/show/G00054MO"},{"id":"A13","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO"},{"id":"A6","pred":"glycosmos_id","subj":"T6","obj":"https://glycosmos.org/glycans/show/G76685HR"},{"id":"A14","pred":"image","subj":"T6","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR"},{"id":"A7","pred":"glycosmos_id","subj":"T7","obj":"https://glycosmos.org/glycans/show/G00054MO"},{"id":"A15","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO"},{"id":"A8","pred":"glycosmos_id","subj":"T8","obj":"https://glycosmos.org/glycans/show/G76685HR"},{"id":"A16","pred":"image","subj":"T8","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR"}],"text":"Molecular cloning of a fourth member of a human alpha (1,3)fucosyltransferase gene family. Multiple homologous sequences that determine expression of the Lewis x, sialyl Lewis x, and difucosyl sialyl Lewis x epitopes.\nWe and others have previously described the isolation of three human alpha (1,3)fucosyltransferase genes which form the basis of a nascent glycosyltransferase gene family. We now report the molecular cloning and expression of a fourth homologous human alpha (1,3)fucosyltransferase gene. When transfected into mammalian cells, this fucosyltransferase gene is capable of directing expression of the Lewis x (Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc), sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 [Fuc alpha 1--\u003e3]GlcNAc), and difucosyl sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc beta 1--\u003e3 Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc) epitopes. The enzyme shares 85% amino acid sequence identity with Fuc-TIII and 89% identity with Fuc-TV but differs substantially in its acceptor substrate requirements. Polymerase chain reaction analyses demonstrate that the gene is syntenic to Fuc-TIII and Fuc-TV on chromosome 19. Southern blot analyses of human genomic DNA demonstrate that these four alpha (1,3)fucosyltransferase genes account for all DNA sequences that cross-hybridize at low stringency with the Fuc-TIII catalytic domain. Using similar methods, a catalytic domain probe from Fuc-TIV identifies a new class of DNA fragments which do not cross-hybridize with the chromosome 19 fucosyltransferase probes. These results extend the molecular definition of a family of human alpha (1,3)fucosyltransferase genes and provide tools for examining fucosyltransferase gene expression."}

{"project":"GlyCosmos-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":154,"end":161},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T2","span":{"begin":163,"end":177},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T3","span":{"begin":193,"end":207},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T4","span":{"begin":616,"end":623},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T5","span":{"begin":665,"end":679},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T6","span":{"begin":754,"end":768},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"}],"attributes":[{"id":"A1","pred":"glycoepitope_id","subj":"T1","obj":"http://www.glycoepitope.jp/epitopes/EP0011"},{"id":"A2","pred":"glycoepitope_id","subj":"T2","obj":"http://www.glycoepitope.jp/epitopes/EP0012"},{"id":"A3","pred":"glycoepitope_id","subj":"T3","obj":"http://www.glycoepitope.jp/epitopes/EP0012"},{"id":"A4","pred":"glycoepitope_id","subj":"T4","obj":"http://www.glycoepitope.jp/epitopes/EP0011"},{"id":"A5","pred":"glycoepitope_id","subj":"T5","obj":"http://www.glycoepitope.jp/epitopes/EP0012"},{"id":"A6","pred":"glycoepitope_id","subj":"T6","obj":"http://www.glycoepitope.jp/epitopes/EP0012"}],"text":"Molecular cloning of a fourth member of a human alpha (1,3)fucosyltransferase gene family. Multiple homologous sequences that determine expression of the Lewis x, sialyl Lewis x, and difucosyl sialyl Lewis x epitopes.\nWe and others have previously described the isolation of three human alpha (1,3)fucosyltransferase genes which form the basis of a nascent glycosyltransferase gene family. We now report the molecular cloning and expression of a fourth homologous human alpha (1,3)fucosyltransferase gene. When transfected into mammalian cells, this fucosyltransferase gene is capable of directing expression of the Lewis x (Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc), sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 [Fuc alpha 1--\u003e3]GlcNAc), and difucosyl sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc beta 1--\u003e3 Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc) epitopes. The enzyme shares 85% amino acid sequence identity with Fuc-TIII and 89% identity with Fuc-TV but differs substantially in its acceptor substrate requirements. Polymerase chain reaction analyses demonstrate that the gene is syntenic to Fuc-TIII and Fuc-TV on chromosome 19. Southern blot analyses of human genomic DNA demonstrate that these four alpha (1,3)fucosyltransferase genes account for all DNA sequences that cross-hybridize at low stringency with the Fuc-TIII catalytic domain. Using similar methods, a catalytic domain probe from Fuc-TIV identifies a new class of DNA fragments which do not cross-hybridize with the chromosome 19 fucosyltransferase probes. These results extend the molecular definition of a family of human alpha (1,3)fucosyltransferase genes and provide tools for examining fucosyltransferase gene expression."}

{"project":"GlyCosmos15-NCBITAXON","denotations":[{"id":"T1","span":{"begin":42,"end":47},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":281,"end":286},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":464,"end":469},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":1186,"end":1191},"obj":"OrganismTaxon"},{"id":"T5","span":{"begin":1614,"end":1619},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9606"},{"id":"A3","pred":"db_id","subj":"T3","obj":"9606"},{"id":"A4","pred":"db_id","subj":"T4","obj":"9606"},{"id":"A5","pred":"db_id","subj":"T5","obj":"9606"}],"text":"Molecular cloning of a fourth member of a human alpha (1,3)fucosyltransferase gene family. Multiple homologous sequences that determine expression of the Lewis x, sialyl Lewis x, and difucosyl sialyl Lewis x epitopes.\nWe and others have previously described the isolation of three human alpha (1,3)fucosyltransferase genes which form the basis of a nascent glycosyltransferase gene family. We now report the molecular cloning and expression of a fourth homologous human alpha (1,3)fucosyltransferase gene. When transfected into mammalian cells, this fucosyltransferase gene is capable of directing expression of the Lewis x (Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc), sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 [Fuc alpha 1--\u003e3]GlcNAc), and difucosyl sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc beta 1--\u003e3 Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc) epitopes. The enzyme shares 85% amino acid sequence identity with Fuc-TIII and 89% identity with Fuc-TV but differs substantially in its acceptor substrate requirements. Polymerase chain reaction analyses demonstrate that the gene is syntenic to Fuc-TIII and Fuc-TV on chromosome 19. Southern blot analyses of human genomic DNA demonstrate that these four alpha (1,3)fucosyltransferase genes account for all DNA sequences that cross-hybridize at low stringency with the Fuc-TIII catalytic domain. Using similar methods, a catalytic domain probe from Fuc-TIV identifies a new class of DNA fragments which do not cross-hybridize with the chromosome 19 fucosyltransferase probes. These results extend the molecular definition of a family of human alpha (1,3)fucosyltransferase genes and provide tools for examining fucosyltransferase gene expression."}

{"project":"GlyCosmos15-UBERON","denotations":[{"id":"T1","span":{"begin":1145,"end":1155},"obj":"Body_part"},{"id":"T2","span":{"begin":1512,"end":1522},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/GO_0005694"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/GO_0005694"}],"text":"Molecular cloning of a fourth member of a human alpha (1,3)fucosyltransferase gene family. Multiple homologous sequences that determine expression of the Lewis x, sialyl Lewis x, and difucosyl sialyl Lewis x epitopes.\nWe and others have previously described the isolation of three human alpha (1,3)fucosyltransferase genes which form the basis of a nascent glycosyltransferase gene family. We now report the molecular cloning and expression of a fourth homologous human alpha (1,3)fucosyltransferase gene. When transfected into mammalian cells, this fucosyltransferase gene is capable of directing expression of the Lewis x (Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc), sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 [Fuc alpha 1--\u003e3]GlcNAc), and difucosyl sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc beta 1--\u003e3 Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc) epitopes. The enzyme shares 85% amino acid sequence identity with Fuc-TIII and 89% identity with Fuc-TV but differs substantially in its acceptor substrate requirements. Polymerase chain reaction analyses demonstrate that the gene is syntenic to Fuc-TIII and Fuc-TV on chromosome 19. Southern blot analyses of human genomic DNA demonstrate that these four alpha (1,3)fucosyltransferase genes account for all DNA sequences that cross-hybridize at low stringency with the Fuc-TIII catalytic domain. Using similar methods, a catalytic domain probe from Fuc-TIV identifies a new class of DNA fragments which do not cross-hybridize with the chromosome 19 fucosyltransferase probes. These results extend the molecular definition of a family of human alpha (1,3)fucosyltransferase genes and provide tools for examining fucosyltransferase gene expression."}

{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":90},"obj":"Sentence"},{"id":"T2","span":{"begin":91,"end":217},"obj":"Sentence"},{"id":"T3","span":{"begin":218,"end":389},"obj":"Sentence"},{"id":"T4","span":{"begin":390,"end":505},"obj":"Sentence"},{"id":"T5","span":{"begin":506,"end":885},"obj":"Sentence"},{"id":"T6","span":{"begin":886,"end":1045},"obj":"Sentence"},{"id":"T7","span":{"begin":1046,"end":1159},"obj":"Sentence"},{"id":"T8","span":{"begin":1160,"end":1372},"obj":"Sentence"},{"id":"T9","span":{"begin":1373,"end":1552},"obj":"Sentence"},{"id":"T10","span":{"begin":1553,"end":1723},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":90},"obj":"Sentence"},{"id":"T2","span":{"begin":91,"end":217},"obj":"Sentence"},{"id":"T3","span":{"begin":218,"end":389},"obj":"Sentence"},{"id":"T4","span":{"begin":390,"end":505},"obj":"Sentence"},{"id":"T5","span":{"begin":506,"end":885},"obj":"Sentence"},{"id":"T6","span":{"begin":886,"end":1045},"obj":"Sentence"},{"id":"T7","span":{"begin":1046,"end":1159},"obj":"Sentence"},{"id":"T8","span":{"begin":1160,"end":1372},"obj":"Sentence"},{"id":"T9","span":{"begin":1373,"end":1552},"obj":"Sentence"},{"id":"T10","span":{"begin":1553,"end":1723},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Molecular cloning of a fourth member of a human alpha (1,3)fucosyltransferase gene family. Multiple homologous sequences that determine expression of the Lewis x, sialyl Lewis x, and difucosyl sialyl Lewis x epitopes.\nWe and others have previously described the isolation of three human alpha (1,3)fucosyltransferase genes which form the basis of a nascent glycosyltransferase gene family. We now report the molecular cloning and expression of a fourth homologous human alpha (1,3)fucosyltransferase gene. When transfected into mammalian cells, this fucosyltransferase gene is capable of directing expression of the Lewis x (Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc), sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 [Fuc alpha 1--\u003e3]GlcNAc), and difucosyl sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc beta 1--\u003e3 Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc) epitopes. The enzyme shares 85% amino acid sequence identity with Fuc-TIII and 89% identity with Fuc-TV but differs substantially in its acceptor substrate requirements. Polymerase chain reaction analyses demonstrate that the gene is syntenic to Fuc-TIII and Fuc-TV on chromosome 19. Southern blot analyses of human genomic DNA demonstrate that these four alpha (1,3)fucosyltransferase genes account for all DNA sequences that cross-hybridize at low stringency with the Fuc-TIII catalytic domain. Using similar methods, a catalytic domain probe from Fuc-TIV identifies a new class of DNA fragments which do not cross-hybridize with the chromosome 19 fucosyltransferase probes. These results extend the molecular definition of a family of human alpha (1,3)fucosyltransferase genes and provide tools for examining fucosyltransferase gene expression."}

{"project":"GlyCosmos15-Sentences","blocks":[{"id":"T1","span":{"begin":0,"end":90},"obj":"Sentence"},{"id":"T2","span":{"begin":91,"end":217},"obj":"Sentence"},{"id":"T3","span":{"begin":218,"end":389},"obj":"Sentence"},{"id":"T4","span":{"begin":390,"end":505},"obj":"Sentence"},{"id":"T5","span":{"begin":506,"end":885},"obj":"Sentence"},{"id":"T6","span":{"begin":886,"end":1045},"obj":"Sentence"},{"id":"T7","span":{"begin":1046,"end":1159},"obj":"Sentence"},{"id":"T8","span":{"begin":1160,"end":1372},"obj":"Sentence"},{"id":"T9","span":{"begin":1373,"end":1552},"obj":"Sentence"},{"id":"T10","span":{"begin":1553,"end":1723},"obj":"Sentence"}],"text":"Molecular cloning of a fourth member of a human alpha (1,3)fucosyltransferase gene family. Multiple homologous sequences that determine expression of the Lewis x, sialyl Lewis x, and difucosyl sialyl Lewis x epitopes.\nWe and others have previously described the isolation of three human alpha (1,3)fucosyltransferase genes which form the basis of a nascent glycosyltransferase gene family. We now report the molecular cloning and expression of a fourth homologous human alpha (1,3)fucosyltransferase gene. When transfected into mammalian cells, this fucosyltransferase gene is capable of directing expression of the Lewis x (Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc), sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 [Fuc alpha 1--\u003e3]GlcNAc), and difucosyl sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc beta 1--\u003e3 Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc) epitopes. The enzyme shares 85% amino acid sequence identity with Fuc-TIII and 89% identity with Fuc-TV but differs substantially in its acceptor substrate requirements. Polymerase chain reaction analyses demonstrate that the gene is syntenic to Fuc-TIII and Fuc-TV on chromosome 19. Southern blot analyses of human genomic DNA demonstrate that these four alpha (1,3)fucosyltransferase genes account for all DNA sequences that cross-hybridize at low stringency with the Fuc-TIII catalytic domain. Using similar methods, a catalytic domain probe from Fuc-TIV identifies a new class of DNA fragments which do not cross-hybridize with the chromosome 19 fucosyltransferase probes. These results extend the molecular definition of a family of human alpha (1,3)fucosyltransferase genes and provide tools for examining fucosyltransferase gene expression."}

{"project":"GlyCosmos15-Glycan","denotations":[{"id":"T1","span":{"begin":154,"end":161},"obj":"Glycan"},{"id":"T2","span":{"begin":163,"end":177},"obj":"Glycan"},{"id":"T3","span":{"begin":193,"end":207},"obj":"Glycan"},{"id":"T4","span":{"begin":616,"end":623},"obj":"Glycan"},{"id":"T5","span":{"begin":665,"end":679},"obj":"Glycan"},{"id":"T6","span":{"begin":681,"end":687},"obj":"Glycan"},{"id":"T7","span":{"begin":754,"end":768},"obj":"Glycan"},{"id":"T8","span":{"begin":770,"end":776},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G00051MO"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G00054MO"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G00054MO"},{"id":"A4","pred":"glycosmos_id","subj":"T4","obj":"https://glycosmos.org/glycans/show/G00051MO"},{"id":"A5","pred":"glycosmos_id","subj":"T5","obj":"https://glycosmos.org/glycans/show/G00054MO"},{"id":"A6","pred":"glycosmos_id","subj":"T6","obj":"https://glycosmos.org/glycans/show/G76685HR"},{"id":"A7","pred":"glycosmos_id","subj":"T7","obj":"https://glycosmos.org/glycans/show/G00054MO"},{"id":"A8","pred":"glycosmos_id","subj":"T8","obj":"https://glycosmos.org/glycans/show/G76685HR"},{"id":"A9","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00051MO"},{"id":"A10","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO"},{"id":"A11","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO"},{"id":"A12","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00051MO"},{"id":"A13","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO"},{"id":"A14","pred":"image","subj":"T6","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR"},{"id":"A15","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO"},{"id":"A16","pred":"image","subj":"T8","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR"}],"text":"Molecular cloning of a fourth member of a human alpha (1,3)fucosyltransferase gene family. Multiple homologous sequences that determine expression of the Lewis x, sialyl Lewis x, and difucosyl sialyl Lewis x epitopes.\nWe and others have previously described the isolation of three human alpha (1,3)fucosyltransferase genes which form the basis of a nascent glycosyltransferase gene family. We now report the molecular cloning and expression of a fourth homologous human alpha (1,3)fucosyltransferase gene. When transfected into mammalian cells, this fucosyltransferase gene is capable of directing expression of the Lewis x (Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc), sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 [Fuc alpha 1--\u003e3]GlcNAc), and difucosyl sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc beta 1--\u003e3 Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc) epitopes. The enzyme shares 85% amino acid sequence identity with Fuc-TIII and 89% identity with Fuc-TV but differs substantially in its acceptor substrate requirements. Polymerase chain reaction analyses demonstrate that the gene is syntenic to Fuc-TIII and Fuc-TV on chromosome 19. Southern blot analyses of human genomic DNA demonstrate that these four alpha (1,3)fucosyltransferase genes account for all DNA sequences that cross-hybridize at low stringency with the Fuc-TIII catalytic domain. Using similar methods, a catalytic domain probe from Fuc-TIV identifies a new class of DNA fragments which do not cross-hybridize with the chromosome 19 fucosyltransferase probes. These results extend the molecular definition of a family of human alpha (1,3)fucosyltransferase genes and provide tools for examining fucosyltransferase gene expression."}

{"project":"GlyCosmos15-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":154,"end":161},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T2","span":{"begin":163,"end":177},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T3","span":{"begin":193,"end":207},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T4","span":{"begin":616,"end":623},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T5","span":{"begin":665,"end":679},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T6","span":{"begin":754,"end":768},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"}],"attributes":[{"id":"A1","pred":"glycoepitope_id","subj":"T1","obj":"http://www.glycoepitope.jp/epitopes/EP0011"},{"id":"A2","pred":"glycoepitope_id","subj":"T2","obj":"http://www.glycoepitope.jp/epitopes/EP0012"},{"id":"A3","pred":"glycoepitope_id","subj":"T3","obj":"http://www.glycoepitope.jp/epitopes/EP0012"},{"id":"A4","pred":"glycoepitope_id","subj":"T4","obj":"http://www.glycoepitope.jp/epitopes/EP0011"},{"id":"A5","pred":"glycoepitope_id","subj":"T5","obj":"http://www.glycoepitope.jp/epitopes/EP0012"},{"id":"A6","pred":"glycoepitope_id","subj":"T6","obj":"http://www.glycoepitope.jp/epitopes/EP0012"}],"text":"Molecular cloning of a fourth member of a human alpha (1,3)fucosyltransferase gene family. Multiple homologous sequences that determine expression of the Lewis x, sialyl Lewis x, and difucosyl sialyl Lewis x epitopes.\nWe and others have previously described the isolation of three human alpha (1,3)fucosyltransferase genes which form the basis of a nascent glycosyltransferase gene family. We now report the molecular cloning and expression of a fourth homologous human alpha (1,3)fucosyltransferase gene. When transfected into mammalian cells, this fucosyltransferase gene is capable of directing expression of the Lewis x (Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc), sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 [Fuc alpha 1--\u003e3]GlcNAc), and difucosyl sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc beta 1--\u003e3 Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc) epitopes. The enzyme shares 85% amino acid sequence identity with Fuc-TIII and 89% identity with Fuc-TV but differs substantially in its acceptor substrate requirements. Polymerase chain reaction analyses demonstrate that the gene is syntenic to Fuc-TIII and Fuc-TV on chromosome 19. Southern blot analyses of human genomic DNA demonstrate that these four alpha (1,3)fucosyltransferase genes account for all DNA sequences that cross-hybridize at low stringency with the Fuc-TIII catalytic domain. Using similar methods, a catalytic domain probe from Fuc-TIV identifies a new class of DNA fragments which do not cross-hybridize with the chromosome 19 fucosyltransferase probes. These results extend the molecular definition of a family of human alpha (1,3)fucosyltransferase genes and provide tools for examining fucosyltransferase gene expression."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":42,"end":47},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":281,"end":286},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":464,"end":469},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":1186,"end":1191},"obj":"OrganismTaxon"},{"id":"T5","span":{"begin":1614,"end":1619},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9606"},{"id":"A3","pred":"db_id","subj":"T3","obj":"9606"},{"id":"A4","pred":"db_id","subj":"T4","obj":"9606"},{"id":"A5","pred":"db_id","subj":"T5","obj":"9606"}],"text":"Molecular cloning of a fourth member of a human alpha (1,3)fucosyltransferase gene family. Multiple homologous sequences that determine expression of the Lewis x, sialyl Lewis x, and difucosyl sialyl Lewis x epitopes.\nWe and others have previously described the isolation of three human alpha (1,3)fucosyltransferase genes which form the basis of a nascent glycosyltransferase gene family. We now report the molecular cloning and expression of a fourth homologous human alpha (1,3)fucosyltransferase gene. When transfected into mammalian cells, this fucosyltransferase gene is capable of directing expression of the Lewis x (Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc), sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 [Fuc alpha 1--\u003e3]GlcNAc), and difucosyl sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc beta 1--\u003e3 Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc) epitopes. The enzyme shares 85% amino acid sequence identity with Fuc-TIII and 89% identity with Fuc-TV but differs substantially in its acceptor substrate requirements. Polymerase chain reaction analyses demonstrate that the gene is syntenic to Fuc-TIII and Fuc-TV on chromosome 19. Southern blot analyses of human genomic DNA demonstrate that these four alpha (1,3)fucosyltransferase genes account for all DNA sequences that cross-hybridize at low stringency with the Fuc-TIII catalytic domain. Using similar methods, a catalytic domain probe from Fuc-TIV identifies a new class of DNA fragments which do not cross-hybridize with the chromosome 19 fucosyltransferase probes. These results extend the molecular definition of a family of human alpha (1,3)fucosyltransferase genes and provide tools for examining fucosyltransferase gene expression."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":1145,"end":1155},"obj":"Body_part"},{"id":"T2","span":{"begin":1512,"end":1522},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/GO_0005694"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/GO_0005694"}],"text":"Molecular cloning of a fourth member of a human alpha (1,3)fucosyltransferase gene family. Multiple homologous sequences that determine expression of the Lewis x, sialyl Lewis x, and difucosyl sialyl Lewis x epitopes.\nWe and others have previously described the isolation of three human alpha (1,3)fucosyltransferase genes which form the basis of a nascent glycosyltransferase gene family. We now report the molecular cloning and expression of a fourth homologous human alpha (1,3)fucosyltransferase gene. When transfected into mammalian cells, this fucosyltransferase gene is capable of directing expression of the Lewis x (Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc), sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4 [Fuc alpha 1--\u003e3]GlcNAc), and difucosyl sialyl Lewis x (NeuNAc alpha 2--\u003e3Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc beta 1--\u003e3 Gal beta 1--\u003e4[Fuc alpha 1--\u003e3]GlcNAc) epitopes. The enzyme shares 85% amino acid sequence identity with Fuc-TIII and 89% identity with Fuc-TV but differs substantially in its acceptor substrate requirements. Polymerase chain reaction analyses demonstrate that the gene is syntenic to Fuc-TIII and Fuc-TV on chromosome 19. Southern blot analyses of human genomic DNA demonstrate that these four alpha (1,3)fucosyltransferase genes account for all DNA sequences that cross-hybridize at low stringency with the Fuc-TIII catalytic domain. Using similar methods, a catalytic domain probe from Fuc-TIV identifies a new class of DNA fragments which do not cross-hybridize with the chromosome 19 fucosyltransferase probes. These results extend the molecular definition of a family of human alpha (1,3)fucosyltransferase genes and provide tools for examining fucosyltransferase gene expression."}