PubMed:1327803 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/1327803","sourcedb":"PubMed","sourceid":"1327803","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/1327803","text":"Leukotriene B4 transcriptionally activates interleukin-6 expression involving NK-chi B and NF-IL6.\nLeukotriene B4 (LTB4) is a notable participant in inflammation and chemotaxis. It is, however, still unclear whether LTB4 acts in this regard directly or indirectly by stimulating the release of chemotactic and inflammatory cytokines. Here we report that LTB4 induces synthesis of interleukin (IL)-6 by human blood monocytes through transcriptional activation of the IL-6 gene. We furthermore demonstrate that this process involves activation of the transcription factor NF-chi B and, to a lesser extent, of NF-IL6, while the activity of the transcription factor AP-1, shown to otherwise confer IL-6 inducibility, appeared to be unaffected by LTB4. Involvement of NF-chi B and NF-IL6 in induction of IL-6 transcription by monocytes was demonstrated using deleted forms of the IL-6 promoter. Activation of the IL-6 promoter by LTB4 was not only associated with accumulation of the respective transcripts but resulted in synthesis of functional IL-6 protein as well. In addition, LTB4 mediated transactivation of a heterologous promoter construct containing the NF-chi B or the NF-IL6 enhancer, but not the AP-1 enhancer. The signaling events mediating this effect appeared to involve the release of H2O2, since LTB4 failed to induce NF-chi B or NF-IL6 in the presence of the scavenger of H2O2, N-acetyl-L-cysteine.","tracks":[{"project":"Inflammaging","denotations":[{"id":"T1","span":{"begin":0,"end":98},"obj":"Sentence"},{"id":"T2","span":{"begin":99,"end":177},"obj":"Sentence"},{"id":"T3","span":{"begin":178,"end":333},"obj":"Sentence"},{"id":"T4","span":{"begin":334,"end":476},"obj":"Sentence"},{"id":"T5","span":{"begin":477,"end":747},"obj":"Sentence"},{"id":"T6","span":{"begin":748,"end":889},"obj":"Sentence"},{"id":"T7","span":{"begin":890,"end":1063},"obj":"Sentence"},{"id":"T8","span":{"begin":1064,"end":1218},"obj":"Sentence"},{"id":"T9","span":{"begin":1219,"end":1412},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":98},"obj":"Sentence"},{"id":"T2","span":{"begin":99,"end":177},"obj":"Sentence"},{"id":"T3","span":{"begin":178,"end":333},"obj":"Sentence"},{"id":"T4","span":{"begin":334,"end":476},"obj":"Sentence"},{"id":"T5","span":{"begin":477,"end":747},"obj":"Sentence"},{"id":"T6","span":{"begin":748,"end":889},"obj":"Sentence"},{"id":"T7","span":{"begin":890,"end":1063},"obj":"Sentence"},{"id":"T8","span":{"begin":1064,"end":1218},"obj":"Sentence"},{"id":"T9","span":{"begin":1219,"end":1412},"obj":"Sentence"}],"attributes":[{"subj":"T1","pred":"source","obj":"Inflammaging"},{"subj":"T2","pred":"source","obj":"Inflammaging"},{"subj":"T3","pred":"source","obj":"Inflammaging"},{"subj":"T4","pred":"source","obj":"Inflammaging"},{"subj":"T5","pred":"source","obj":"Inflammaging"},{"subj":"T6","pred":"source","obj":"Inflammaging"},{"subj":"T7","pred":"source","obj":"Inflammaging"},{"subj":"T8","pred":"source","obj":"Inflammaging"},{"subj":"T9","pred":"source","obj":"Inflammaging"},{"subj":"T1","pred":"source","obj":"Inflammaging"},{"subj":"T2","pred":"source","obj":"Inflammaging"},{"subj":"T3","pred":"source","obj":"Inflammaging"},{"subj":"T4","pred":"source","obj":"Inflammaging"},{"subj":"T5","pred":"source","obj":"Inflammaging"},{"subj":"T6","pred":"source","obj":"Inflammaging"},{"subj":"T7","pred":"source","obj":"Inflammaging"},{"subj":"T8","pred":"source","obj":"Inflammaging"},{"subj":"T9","pred":"source","obj":"Inflammaging"}]},{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":43,"end":56},"obj":"protein"},{"id":"T2","span":{"begin":78,"end":86},"obj":"protein"},{"id":"T3","span":{"begin":91,"end":97},"obj":"protein"},{"id":"T4","span":{"begin":310,"end":332},"obj":"protein"},{"id":"T5","span":{"begin":380,"end":398},"obj":"protein"},{"id":"T6","span":{"begin":402,"end":423},"obj":"cell_type"},{"id":"T7","span":{"begin":466,"end":475},"obj":"DNA"},{"id":"T8","span":{"begin":549,"end":569},"obj":"protein"},{"id":"T9","span":{"begin":570,"end":578},"obj":"protein"},{"id":"T10","span":{"begin":607,"end":613},"obj":"protein"},{"id":"T11","span":{"begin":641,"end":661},"obj":"protein"},{"id":"T12","span":{"begin":662,"end":666},"obj":"protein"},{"id":"T13","span":{"begin":694,"end":698},"obj":"protein"},{"id":"T14","span":{"begin":763,"end":771},"obj":"protein"},{"id":"T15","span":{"begin":776,"end":782},"obj":"protein"},{"id":"T16","span":{"begin":799,"end":803},"obj":"protein"},{"id":"T17","span":{"begin":875,"end":888},"obj":"DNA"},{"id":"T18","span":{"begin":908,"end":921},"obj":"DNA"},{"id":"T19","span":{"begin":1042,"end":1054},"obj":"protein"},{"id":"T20","span":{"begin":1112,"end":1143},"obj":"DNA"},{"id":"T21","span":{"begin":1159,"end":1167},"obj":"DNA"},{"id":"T22","span":{"begin":1175,"end":1190},"obj":"DNA"},{"id":"T23","span":{"begin":1204,"end":1217},"obj":"DNA"},{"id":"T24","span":{"begin":1331,"end":1339},"obj":"protein"},{"id":"T25","span":{"begin":1343,"end":1349},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"jnlpba-st-training"},{"subj":"T2","pred":"source","obj":"jnlpba-st-training"},{"subj":"T3","pred":"source","obj":"jnlpba-st-training"},{"subj":"T4","pred":"source","obj":"jnlpba-st-training"},{"subj":"T5","pred":"source","obj":"jnlpba-st-training"},{"subj":"T6","pred":"source","obj":"jnlpba-st-training"},{"subj":"T7","pred":"source","obj":"jnlpba-st-training"},{"subj":"T8","pred":"source","obj":"jnlpba-st-training"},{"subj":"T9","pred":"source","obj":"jnlpba-st-training"},{"subj":"T10","pred":"source","obj":"jnlpba-st-training"},{"subj":"T11","pred":"source","obj":"jnlpba-st-training"},{"subj":"T12","pred":"source","obj":"jnlpba-st-training"},{"subj":"T13","pred":"source","obj":"jnlpba-st-training"},{"subj":"T14","pred":"source","obj":"jnlpba-st-training"},{"subj":"T15","pred":"source","obj":"jnlpba-st-training"},{"subj":"T16","pred":"source","obj":"jnlpba-st-training"},{"subj":"T17","pred":"source","obj":"jnlpba-st-training"},{"subj":"T18","pred":"source","obj":"jnlpba-st-training"},{"subj":"T19","pred":"source","obj":"jnlpba-st-training"},{"subj":"T20","pred":"source","obj":"jnlpba-st-training"},{"subj":"T21","pred":"source","obj":"jnlpba-st-training"},{"subj":"T22","pred":"source","obj":"jnlpba-st-training"},{"subj":"T23","pred":"source","obj":"jnlpba-st-training"},{"subj":"T24","pred":"source","obj":"jnlpba-st-training"},{"subj":"T25","pred":"source","obj":"jnlpba-st-training"}]},{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":98},"obj":"Sentence"},{"id":"S2","span":{"begin":99,"end":177},"obj":"Sentence"},{"id":"S3","span":{"begin":178,"end":333},"obj":"Sentence"},{"id":"S4","span":{"begin":334,"end":476},"obj":"Sentence"},{"id":"S5","span":{"begin":477,"end":747},"obj":"Sentence"},{"id":"S6","span":{"begin":748,"end":889},"obj":"Sentence"},{"id":"S7","span":{"begin":890,"end":1063},"obj":"Sentence"},{"id":"S8","span":{"begin":1064,"end":1218},"obj":"Sentence"},{"id":"S9","span":{"begin":1219,"end":1412},"obj":"Sentence"}],"attributes":[{"subj":"S1","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S2","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S3","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S4","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S5","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S6","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S7","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S8","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S9","pred":"source","obj":"pubmed-sentences-benchmark"}]},{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":0,"end":14},"obj":"NP"},{"id":"C2","span":{"begin":43,"end":56},"obj":"NP"},{"id":"C3","span":{"begin":78,"end":86},"obj":"NP"},{"id":"C4","span":{"begin":91,"end":97},"obj":"NP"},{"id":"C5","span":{"begin":99,"end":120},"obj":"NP"},{"id":"C6","span":{"begin":216,"end":220},"obj":"NP"},{"id":"C7","span":{"begin":354,"end":358},"obj":"NP"},{"id":"C8","span":{"begin":380,"end":398},"obj":"NP"},{"id":"C9","span":{"begin":402,"end":423},"obj":"NP"},{"id":"C11","span":{"begin":466,"end":470},"obj":"NP"},{"id":"C10","span":{"begin":432,"end":475},"obj":"NP"},{"id":"C12","span":{"begin":509,"end":521},"obj":"NP"},{"id":"C13","span":{"begin":545,"end":578},"obj":"NP"},{"id":"C14","span":{"begin":607,"end":613},"obj":"NP"},{"id":"C15","span":{"begin":637,"end":666},"obj":"NP"},{"id":"C16","span":{"begin":694,"end":698},"obj":"NP"},{"id":"C17","span":{"begin":742,"end":746},"obj":"NP"},{"id":"C18","span":{"begin":763,"end":771},"obj":"NP"},{"id":"C19","span":{"begin":776,"end":782},"obj":"NP"},{"id":"C20","span":{"begin":799,"end":803},"obj":"NP"},{"id":"C21","span":{"begin":821,"end":830},"obj":"NP"},{"id":"C23","span":{"begin":875,"end":879},"obj":"NP"},{"id":"C22","span":{"begin":871,"end":888},"obj":"NP"},{"id":"C24","span":{"begin":904,"end":921},"obj":"NP"},{"id":"C25","span":{"begin":925,"end":929},"obj":"NP"},{"id":"C26","span":{"begin":1042,"end":1046},"obj":"NP"},{"id":"C27","span":{"begin":1077,"end":1081},"obj":"NP"},{"id":"C28","span":{"begin":1159,"end":1167},"obj":"NP"},{"id":"C29","span":{"begin":1175,"end":1181},"obj":"NP"},{"id":"C30","span":{"begin":1204,"end":1208},"obj":"NP"},{"id":"C31","span":{"begin":1297,"end":1301},"obj":"NP"},{"id":"C32","span":{"begin":1309,"end":1313},"obj":"NP"},{"id":"C33","span":{"begin":1331,"end":1339},"obj":"NP"},{"id":"C34","span":{"begin":1343,"end":1349},"obj":"NP"},{"id":"C36","span":{"begin":1386,"end":1390},"obj":"NP"},{"id":"C35","span":{"begin":1369,"end":1390},"obj":"NP"},{"id":"C37","span":{"begin":1392,"end":1411},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C5","obj":"C1"},{"id":"R2","pred":"coref-ident","subj":"C6","obj":"C5"},{"id":"R3","pred":"coref-ident","subj":"C7","obj":"C6"},{"id":"R4","pred":"coref-ident","subj":"C8","obj":"C2"},{"id":"R5","pred":"coref-ident","subj":"C11","obj":"C8"},{"id":"R6","pred":"coref-ident","subj":"C12","obj":"C10"},{"id":"R7","pred":"coref-ident","subj":"C13","obj":"C3"},{"id":"R8","pred":"coref-ident","subj":"C14","obj":"C4"},{"id":"R9","pred":"coref-ident","subj":"C16","obj":"C11"},{"id":"R10","pred":"coref-ident","subj":"C17","obj":"C7"},{"id":"R11","pred":"coref-ident","subj":"C18","obj":"C13"},{"id":"R12","pred":"coref-ident","subj":"C19","obj":"C14"},{"id":"R13","pred":"coref-ident","subj":"C20","obj":"C16"},{"id":"R14","pred":"coref-other","subj":"C21","obj":"C9"},{"id":"R15","pred":"coref-ident","subj":"C23","obj":"C20"},{"id":"R16","pred":"coref-ident","subj":"C24","obj":"C22"},{"id":"R17","pred":"coref-ident","subj":"C25","obj":"C17"},{"id":"R18","pred":"coref-ident","subj":"C26","obj":"C23"},{"id":"R19","pred":"coref-ident","subj":"C27","obj":"C25"},{"id":"R20","pred":"coref-ident","subj":"C28","obj":"C18"},{"id":"R21","pred":"coref-ident","subj":"C29","obj":"C19"},{"id":"R22","pred":"coref-ident","subj":"C30","obj":"C15"},{"id":"R23","pred":"coref-ident","subj":"C32","obj":"C27"},{"id":"R24","pred":"coref-ident","subj":"C33","obj":"C28"},{"id":"R25","pred":"coref-ident","subj":"C34","obj":"C29"},{"id":"R26","pred":"coref-ident","subj":"C36","obj":"C31"},{"id":"R27","pred":"coref-appos","subj":"C37","obj":"C35"}],"attributes":[{"subj":"C1","pred":"source","obj":"genia-medco-coref"},{"subj":"C2","pred":"source","obj":"genia-medco-coref"},{"subj":"C3","pred":"source","obj":"genia-medco-coref"},{"subj":"C4","pred":"source","obj":"genia-medco-coref"},{"subj":"C5","pred":"source","obj":"genia-medco-coref"},{"subj":"C6","pred":"source","obj":"genia-medco-coref"},{"subj":"C7","pred":"source","obj":"genia-medco-coref"},{"subj":"C8","pred":"source","obj":"genia-medco-coref"},{"subj":"C9","pred":"source","obj":"genia-medco-coref"},{"subj":"C11","pred":"source","obj":"genia-medco-coref"},{"subj":"C10","pred":"source","obj":"genia-medco-coref"},{"subj":"C12","pred":"source","obj":"genia-medco-coref"},{"subj":"C13","pred":"source","obj":"genia-medco-coref"},{"subj":"C14","pred":"source","obj":"genia-medco-coref"},{"subj":"C15","pred":"source","obj":"genia-medco-coref"},{"subj":"C16","pred":"source","obj":"genia-medco-coref"},{"subj":"C17","pred":"source","obj":"genia-medco-coref"},{"subj":"C18","pred":"source","obj":"genia-medco-coref"},{"subj":"C19","pred":"source","obj":"genia-medco-coref"},{"subj":"C20","pred":"source","obj":"genia-medco-coref"},{"subj":"C21","pred":"source","obj":"genia-medco-coref"},{"subj":"C23","pred":"source","obj":"genia-medco-coref"},{"subj":"C22","pred":"source","obj":"genia-medco-coref"},{"subj":"C24","pred":"source","obj":"genia-medco-coref"},{"subj":"C25","pred":"source","obj":"genia-medco-coref"},{"subj":"C26","pred":"source","obj":"genia-medco-coref"},{"subj":"C27","pred":"source","obj":"genia-medco-coref"},{"subj":"C28","pred":"source","obj":"genia-medco-coref"},{"subj":"C29","pred":"source","obj":"genia-medco-coref"},{"subj":"C30","pred":"source","obj":"genia-medco-coref"},{"subj":"C31","pred":"source","obj":"genia-medco-coref"},{"subj":"C32","pred":"source","obj":"genia-medco-coref"},{"subj":"C33","pred":"source","obj":"genia-medco-coref"},{"subj":"C34","pred":"source","obj":"genia-medco-coref"},{"subj":"C36","pred":"source","obj":"genia-medco-coref"},{"subj":"C35","pred":"source","obj":"genia-medco-coref"},{"subj":"C37","pred":"source","obj":"genia-medco-coref"}]},{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":0,"end":14},"obj":"other_organic_compound"},{"id":"T2","span":{"begin":43,"end":56},"obj":"protein_molecule"},{"id":"T3","span":{"begin":78,"end":86},"obj":"protein_molecule"},{"id":"T4","span":{"begin":91,"end":97},"obj":"protein_molecule"},{"id":"T5","span":{"begin":99,"end":113},"obj":"other_organic_compound"},{"id":"T6","span":{"begin":115,"end":119},"obj":"other_organic_compound"},{"id":"T7","span":{"begin":149,"end":161},"obj":"other_name"},{"id":"T8","span":{"begin":166,"end":176},"obj":"other_name"},{"id":"T9","span":{"begin":216,"end":220},"obj":"other_organic_compound"},{"id":"T10","span":{"begin":294,"end":309},"obj":"other_name"},{"id":"T11","span":{"begin":310,"end":332},"obj":"protein_family_or_group"},{"id":"T12","span":{"begin":354,"end":358},"obj":"other_organic_compound"},{"id":"T13","span":{"begin":380,"end":398},"obj":"protein_molecule"},{"id":"T14","span":{"begin":402,"end":423},"obj":"cell_type"},{"id":"T15","span":{"begin":432,"end":458},"obj":"other_name"},{"id":"T16","span":{"begin":466,"end":470},"obj":"protein_molecule"},{"id":"T17","span":{"begin":549,"end":569},"obj":"protein_family_or_group"},{"id":"T18","span":{"begin":570,"end":578},"obj":"protein_molecule"},{"id":"T19","span":{"begin":607,"end":613},"obj":"protein_molecule"},{"id":"T20","span":{"begin":641,"end":661},"obj":"protein_family_or_group"},{"id":"T21","span":{"begin":662,"end":666},"obj":"protein_molecule"},{"id":"T22","span":{"begin":694,"end":698},"obj":"protein_molecule"},{"id":"T23","span":{"begin":742,"end":746},"obj":"other_organic_compound"},{"id":"T24","span":{"begin":763,"end":771},"obj":"protein_molecule"},{"id":"T25","span":{"begin":776,"end":782},"obj":"protein_molecule"},{"id":"T26","span":{"begin":799,"end":803},"obj":"protein_molecule"},{"id":"T27","span":{"begin":875,"end":879},"obj":"protein_molecule"},{"id":"T28","span":{"begin":908,"end":912},"obj":"protein_molecule"},{"id":"T29","span":{"begin":925,"end":929},"obj":"other_organic_compound"},{"id":"T30","span":{"begin":1042,"end":1046},"obj":"protein_molecule"},{"id":"T31","span":{"begin":1077,"end":1081},"obj":"other_organic_compound"},{"id":"T32","span":{"begin":1112,"end":1143},"obj":"DNA_molecule"},{"id":"T33","span":{"begin":1159,"end":1167},"obj":"protein_molecule"},{"id":"T34","span":{"begin":1175,"end":1181},"obj":"protein_molecule"},{"id":"T35","span":{"begin":1204,"end":1208},"obj":"protein_molecule"},{"id":"T36","span":{"begin":1297,"end":1301},"obj":"inorganic"},{"id":"T37","span":{"begin":1309,"end":1313},"obj":"other_organic_compound"},{"id":"T38","span":{"begin":1331,"end":1339},"obj":"protein_molecule"},{"id":"T39","span":{"begin":1343,"end":1349},"obj":"protein_molecule"},{"id":"T40","span":{"begin":1386,"end":1390},"obj":"inorganic"},{"id":"T41","span":{"begin":1392,"end":1411},"obj":"amino_acid_monomer"}],"attributes":[{"subj":"T1","pred":"source","obj":"GENIAcorpus"},{"subj":"T2","pred":"source","obj":"GENIAcorpus"},{"subj":"T3","pred":"source","obj":"GENIAcorpus"},{"subj":"T4","pred":"source","obj":"GENIAcorpus"},{"subj":"T5","pred":"source","obj":"GENIAcorpus"},{"subj":"T6","pred":"source","obj":"GENIAcorpus"},{"subj":"T7","pred":"source","obj":"GENIAcorpus"},{"subj":"T8","pred":"source","obj":"GENIAcorpus"},{"subj":"T9","pred":"source","obj":"GENIAcorpus"},{"subj":"T10","pred":"source","obj":"GENIAcorpus"},{"subj":"T11","pred":"source","obj":"GENIAcorpus"},{"subj":"T12","pred":"source","obj":"GENIAcorpus"},{"subj":"T13","pred":"source","obj":"GENIAcorpus"},{"subj":"T14","pred":"source","obj":"GENIAcorpus"},{"subj":"T15","pred":"source","obj":"GENIAcorpus"},{"subj":"T16","pred":"source","obj":"GENIAcorpus"},{"subj":"T17","pred":"source","obj":"GENIAcorpus"},{"subj":"T18","pred":"source","obj":"GENIAcorpus"},{"subj":"T19","pred":"source","obj":"GENIAcorpus"},{"subj":"T20","pred":"source","obj":"GENIAcorpus"},{"subj":"T21","pred":"source","obj":"GENIAcorpus"},{"subj":"T22","pred":"source","obj":"GENIAcorpus"},{"subj":"T23","pred":"source","obj":"GENIAcorpus"},{"subj":"T24","pred":"source","obj":"GENIAcorpus"},{"subj":"T25","pred":"source","obj":"GENIAcorpus"},{"subj":"T26","pred":"source","obj":"GENIAcorpus"},{"subj":"T27","pred":"source","obj":"GENIAcorpus"},{"subj":"T28","pred":"source","obj":"GENIAcorpus"},{"subj":"T29","pred":"source","obj":"GENIAcorpus"},{"subj":"T30","pred":"source","obj":"GENIAcorpus"},{"subj":"T31","pred":"source","obj":"GENIAcorpus"},{"subj":"T32","pred":"source","obj":"GENIAcorpus"},{"subj":"T33","pred":"source","obj":"GENIAcorpus"},{"subj":"T34","pred":"source","obj":"GENIAcorpus"},{"subj":"T35","pred":"source","obj":"GENIAcorpus"},{"subj":"T36","pred":"source","obj":"GENIAcorpus"},{"subj":"T37","pred":"source","obj":"GENIAcorpus"},{"subj":"T38","pred":"source","obj":"GENIAcorpus"},{"subj":"T39","pred":"source","obj":"GENIAcorpus"},{"subj":"T40","pred":"source","obj":"GENIAcorpus"},{"subj":"T41","pred":"source","obj":"GENIAcorpus"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"Inflammaging","color":"#eca493","default":true},{"id":"jnlpba-st-training","color":"#939bec"},{"id":"pubmed-sentences-benchmark","color":"#b5ec93"},{"id":"genia-medco-coref","color":"#ec93cf"},{"id":"GENIAcorpus","color":"#93e9ec"}]}]}}