PubMed:1327574 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/1327574","sourcedb":"PubMed","sourceid":"1327574","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/1327574","text":"Polymicrobial sepsis disrupts normal neutrophil extracellular matrix protein interactions.\nThe purpose of this study was to examine how intra-abdominal sepsis and extracellular matrix proteins (fibronectin, laminin) affect adherent polymorphonuclear leukocyte (PMN) function. Two groups of swine were studied: Group I (n = 5) underwent sham laparotomy; Group II (n = 8) underwent cecal ligation and incision. PMN adherent to either fibronectin (F) or laminin (L) had increased candicidal activity over buffer (B) by Group I but not by post-operative day 8 Group II PMN. (Percent specific release 51Cr-Group I--35.00, 68.25, 64.75% for B, F, and L; P less than 0.001 comparing B vs. F or L; Group II--14.25, 12.50, 12.75% for B, F, and L; P = NS comparing B vs. F or L.) To determine the mechanism for this finding, PMN priming was then assessed by evaluating both PMN adherence to extracellular matrix proteins and the cell surface expression of CR1/CR3 by using sheep RBC opsonized with C3b or C3bi. PMN activation was assayed by using MTT-Formazan, myeloperoxidase, and hypochlorous acid (HOCl) production. Fibronectin and laminin increased PMN adherence and CR1/CR3 expression over buffer by Group I and Group II animals. Fibronectin and laminin increased MTT-Formazan, myeloperoxidase, and HOCl production over buffer by Group I PMN but not POD 8 Group II PMN. These results suggest that untreated intra-abdominal sepsis partially abrogates the effect of extracellular matrix proteins on PMN function; in particular, the activation but not priming of adherent PMN by extracellular matrix proteins is reduced in this clinical situation.","tracks":[{"project":"Inflammaging","denotations":[{"id":"T1","span":{"begin":0,"end":90},"obj":"Sentence"},{"id":"T2","span":{"begin":91,"end":275},"obj":"Sentence"},{"id":"T3","span":{"begin":276,"end":309},"obj":"Sentence"},{"id":"T4","span":{"begin":310,"end":408},"obj":"Sentence"},{"id":"T5","span":{"begin":409,"end":1000},"obj":"Sentence"},{"id":"T6","span":{"begin":1001,"end":1108},"obj":"Sentence"},{"id":"T7","span":{"begin":1109,"end":1224},"obj":"Sentence"},{"id":"T8","span":{"begin":1225,"end":1364},"obj":"Sentence"},{"id":"T9","span":{"begin":1365,"end":1639},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":90},"obj":"Sentence"},{"id":"T2","span":{"begin":91,"end":275},"obj":"Sentence"},{"id":"T3","span":{"begin":276,"end":309},"obj":"Sentence"},{"id":"T4","span":{"begin":310,"end":408},"obj":"Sentence"},{"id":"T5","span":{"begin":409,"end":1000},"obj":"Sentence"},{"id":"T6","span":{"begin":1001,"end":1108},"obj":"Sentence"},{"id":"T7","span":{"begin":1109,"end":1224},"obj":"Sentence"},{"id":"T8","span":{"begin":1225,"end":1364},"obj":"Sentence"},{"id":"T9","span":{"begin":1365,"end":1639},"obj":"Sentence"}],"attributes":[{"subj":"T1","pred":"source","obj":"Inflammaging"},{"subj":"T2","pred":"source","obj":"Inflammaging"},{"subj":"T3","pred":"source","obj":"Inflammaging"},{"subj":"T4","pred":"source","obj":"Inflammaging"},{"subj":"T5","pred":"source","obj":"Inflammaging"},{"subj":"T6","pred":"source","obj":"Inflammaging"},{"subj":"T7","pred":"source","obj":"Inflammaging"},{"subj":"T8","pred":"source","obj":"Inflammaging"},{"subj":"T9","pred":"source","obj":"Inflammaging"},{"subj":"T1","pred":"source","obj":"Inflammaging"},{"subj":"T2","pred":"source","obj":"Inflammaging"},{"subj":"T3","pred":"source","obj":"Inflammaging"},{"subj":"T4","pred":"source","obj":"Inflammaging"},{"subj":"T5","pred":"source","obj":"Inflammaging"},{"subj":"T6","pred":"source","obj":"Inflammaging"},{"subj":"T7","pred":"source","obj":"Inflammaging"},{"subj":"T8","pred":"source","obj":"Inflammaging"},{"subj":"T9","pred":"source","obj":"Inflammaging"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"Inflammaging","color":"#ec93ea","default":true}]}]}}