PubMed:12912968 JSONTXT

{"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T1","span":{"begin":155,"end":636},"obj":"OBJECTIVE"},{"id":"T2","span":{"begin":658,"end":1093},"obj":"METHODS"},{"id":"T3","span":{"begin":1103,"end":1390},"obj":"RESULTS"},{"id":"T4","span":{"begin":1403,"end":1877},"obj":"CONCLUSIONS"}],"text":"Cell-based protein delivery system for the inhibition of the growth of pancreatic cancer: NK4 gene-transduced oral mucosal epithelial cell sheet.\nPURPOSE: Pancreatic resection for pancreatic cancer is the only curative modality, but the high incidence of local recurrence after surgery results in a very poor prognosis. This study aims to develop a new therapeutic tool that could inhibit the growth of remnant cancer cells, which is based on local delivery of NK4 (hepatocyte growth factor antagonist) secreted from an NK4 gene-transduced oral mucosal epithelial cell (OMEC) sheet (NK4-sheet), which is adhered to the resected surface.\nEXPERIMENTAL DESIGN: OMECs, harvested and cultured according to 3T3 feeder layer technique, were seeded on a collagen mesh-overlayered, biodegradable VICRYL mesh to produce an OMEC sheet. NK4 gene transduction was mediated by recombinant adenovirus (Ad-NK4). Applicability of OMECs for cell-based NK4 delivery was examined. An experimental model using nude mice was established to determine the effect of an NK4-sheet on both tumor growth and angiogenesis.\nRESULTS: NK4 secreted from Ad-NK4-transduced OMECs suppressed MRC-5-induced invasion of pancreatic cancer cell lines. Heterotopically implanted gene-transduced OMECs remained for \u003e/==\" BORDER=\"0\"\u003e10 days while gradually decreasing. NK4-sheets inhibited both angiogenesis and tumor growth in vivo.\nCONCLUSION: Autologous OMEC was found to be suited to this purpose because of no secretion of hepatocyte growth factor, ease in harvesting from a patient, reasonably high proliferation potential, and no immune reaction. Although NK4-sheets under development exhibited a low level and short period of NK4 secretion, it is expected that this system may have a great potentiality of protein delivery system to target tissue at clinical situations when it is loaded with multilayered OMECs."}

{"project":"DisGeNET5_gene_disease","denotations":[{"id":"12912968-0#90#93#gene9235","span":{"begin":90,"end":93},"obj":"gene9235"},{"id":"12912968-0#71#88#diseaseC0235974","span":{"begin":71,"end":88},"obj":"diseaseC0235974"},{"id":"12912968-0#71#88#diseaseC0346647","span":{"begin":71,"end":88},"obj":"diseaseC0346647"}],"relations":[{"id":"90#93#gene923571#88#diseaseC0235974","pred":"associated_with","subj":"12912968-0#90#93#gene9235","obj":"12912968-0#71#88#diseaseC0235974"},{"id":"90#93#gene923571#88#diseaseC0346647","pred":"associated_with","subj":"12912968-0#90#93#gene9235","obj":"12912968-0#71#88#diseaseC0346647"}],"text":"Cell-based protein delivery system for the inhibition of the growth of pancreatic cancer: NK4 gene-transduced oral mucosal epithelial cell sheet.\nPURPOSE: Pancreatic resection for pancreatic cancer is the only curative modality, but the high incidence of local recurrence after surgery results in a very poor prognosis. This study aims to develop a new therapeutic tool that could inhibit the growth of remnant cancer cells, which is based on local delivery of NK4 (hepatocyte growth factor antagonist) secreted from an NK4 gene-transduced oral mucosal epithelial cell (OMEC) sheet (NK4-sheet), which is adhered to the resected surface.\nEXPERIMENTAL DESIGN: OMECs, harvested and cultured according to 3T3 feeder layer technique, were seeded on a collagen mesh-overlayered, biodegradable VICRYL mesh to produce an OMEC sheet. NK4 gene transduction was mediated by recombinant adenovirus (Ad-NK4). Applicability of OMECs for cell-based NK4 delivery was examined. An experimental model using nude mice was established to determine the effect of an NK4-sheet on both tumor growth and angiogenesis.\nRESULTS: NK4 secreted from Ad-NK4-transduced OMECs suppressed MRC-5-induced invasion of pancreatic cancer cell lines. Heterotopically implanted gene-transduced OMECs remained for \u003e/==\" BORDER=\"0\"\u003e10 days while gradually decreasing. NK4-sheets inhibited both angiogenesis and tumor growth in vivo.\nCONCLUSION: Autologous OMEC was found to be suited to this purpose because of no secretion of hepatocyte growth factor, ease in harvesting from a patient, reasonably high proliferation potential, and no immune reaction. Although NK4-sheets under development exhibited a low level and short period of NK4 secretion, it is expected that this system may have a great potentiality of protein delivery system to target tissue at clinical situations when it is loaded with multilayered OMECs."}

{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":90,"end":93},"obj":"gene:9235"},{"id":"T1","span":{"begin":71,"end":88},"obj":"disease:C0235974"},{"id":"T2","span":{"begin":90,"end":93},"obj":"gene:9235"},{"id":"T3","span":{"begin":71,"end":88},"obj":"disease:C0346647"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"text":"Cell-based protein delivery system for the inhibition of the growth of pancreatic cancer: NK4 gene-transduced oral mucosal epithelial cell sheet.\nPURPOSE: Pancreatic resection for pancreatic cancer is the only curative modality, but the high incidence of local recurrence after surgery results in a very poor prognosis. This study aims to develop a new therapeutic tool that could inhibit the growth of remnant cancer cells, which is based on local delivery of NK4 (hepatocyte growth factor antagonist) secreted from an NK4 gene-transduced oral mucosal epithelial cell (OMEC) sheet (NK4-sheet), which is adhered to the resected surface.\nEXPERIMENTAL DESIGN: OMECs, harvested and cultured according to 3T3 feeder layer technique, were seeded on a collagen mesh-overlayered, biodegradable VICRYL mesh to produce an OMEC sheet. NK4 gene transduction was mediated by recombinant adenovirus (Ad-NK4). Applicability of OMECs for cell-based NK4 delivery was examined. An experimental model using nude mice was established to determine the effect of an NK4-sheet on both tumor growth and angiogenesis.\nRESULTS: NK4 secreted from Ad-NK4-transduced OMECs suppressed MRC-5-induced invasion of pancreatic cancer cell lines. Heterotopically implanted gene-transduced OMECs remained for \u003e/==\" BORDER=\"0\"\u003e10 days while gradually decreasing. NK4-sheets inhibited both angiogenesis and tumor growth in vivo.\nCONCLUSION: Autologous OMEC was found to be suited to this purpose because of no secretion of hepatocyte growth factor, ease in harvesting from a patient, reasonably high proliferation potential, and no immune reaction. Although NK4-sheets under development exhibited a low level and short period of NK4 secretion, it is expected that this system may have a great potentiality of protein delivery system to target tissue at clinical situations when it is loaded with multilayered OMECs."}