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PubMed:12912968 JSONTXT

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DisGeNET

Id Subject Object Predicate Lexical cue
T0 90-93 gene:9235 denotes NK4
T1 71-88 disease:C0235974 denotes pancreatic cancer
T2 90-93 gene:9235 denotes NK4
T3 71-88 disease:C0346647 denotes pancreatic cancer
R1 T0 T1 associated_with NK4,pancreatic cancer
R2 T2 T3 associated_with NK4,pancreatic cancer

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
12912968-0#90#93#gene9235 90-93 gene9235 denotes NK4
12912968-0#71#88#diseaseC0235974 71-88 diseaseC0235974 denotes pancreatic cancer
12912968-0#71#88#diseaseC0346647 71-88 diseaseC0346647 denotes pancreatic cancer
90#93#gene923571#88#diseaseC0235974 12912968-0#90#93#gene9235 12912968-0#71#88#diseaseC0235974 associated_with NK4,pancreatic cancer
90#93#gene923571#88#diseaseC0346647 12912968-0#90#93#gene9235 12912968-0#71#88#diseaseC0346647 associated_with NK4,pancreatic cancer

PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 155-636 OBJECTIVE denotes Pancreatic resection for pancreatic cancer is the only curative modality, but the high incidence of local recurrence after surgery results in a very poor prognosis. This study aims to develop a new therapeutic tool that could inhibit the growth of remnant cancer cells, which is based on local delivery of NK4 (hepatocyte growth factor antagonist) secreted from an NK4 gene-transduced oral mucosal epithelial cell (OMEC) sheet (NK4-sheet), which is adhered to the resected surface.
T2 658-1093 METHODS denotes OMECs, harvested and cultured according to 3T3 feeder layer technique, were seeded on a collagen mesh-overlayered, biodegradable VICRYL mesh to produce an OMEC sheet. NK4 gene transduction was mediated by recombinant adenovirus (Ad-NK4). Applicability of OMECs for cell-based NK4 delivery was examined. An experimental model using nude mice was established to determine the effect of an NK4-sheet on both tumor growth and angiogenesis.
T3 1103-1390 RESULTS denotes NK4 secreted from Ad-NK4-transduced OMECs suppressed MRC-5-induced invasion of pancreatic cancer cell lines. Heterotopically implanted gene-transduced OMECs remained for >/==" BORDER="0">10 days while gradually decreasing. NK4-sheets inhibited both angiogenesis and tumor growth in vivo.
T4 1403-1877 CONCLUSIONS denotes Autologous OMEC was found to be suited to this purpose because of no secretion of hepatocyte growth factor, ease in harvesting from a patient, reasonably high proliferation potential, and no immune reaction. Although NK4-sheets under development exhibited a low level and short period of NK4 secretion, it is expected that this system may have a great potentiality of protein delivery system to target tissue at clinical situations when it is loaded with multilayered OMECs.