PubMed:12810612 JSONTXT

{"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T1","span":{"begin":92,"end":653},"obj":"BACKGROUND"},{"id":"T2","span":{"begin":675,"end":1430},"obj":"RESULTS"},{"id":"T3","span":{"begin":1444,"end":1677},"obj":"CONCLUSIONS"}],"text":"Increased expression of thrombospondin-1 in vessel wall of diabetic Zucker rat.\nBACKGROUND: Thrombospondin-1 (TSP-1) expression in the vascular wall has been related to the development of atherosclerotic lesions and restenosis. TSP-1 promotes the development of neointima and has recently been associated with atherogenesis at a genetic level. Because TSP-1 expression is responsive to glucose stimulation in mesangial cells, we hypothesized that glucose may stimulate its production by vascular cells. Thus, TSP-1 expression in the blood vessel wall may increase, providing a molecular link between diabetes and accelerated vascular lesion development.\nMETHODS AND RESULTS: To determine whether the expression level of TSP-1 in vessel wall is increased in diabetes, aorta and carotid arteries of Zucker rats were used for immunostaining, Western blotting, and in situ RNA hybridization. A significant increase in TSP-1 expression was found in the adventitia of blood vessels from diabetic rats. Consistent with the well-known antiangiogenic effect of TSP-1, the number of vasa vasorum was reduced in aortas from diabetic rats. In cultured endothelial cells, vascular smooth muscle cells, and fibroblasts, TSP-1 expression increased in response to glucose stimulation (\u003e30-fold). After balloon catheter injury to carotid arteries, expression of TSP-1 protein and mRNA was higher at all time points in the vessels of diabetic rats.\nCONCLUSIONS: Increased expression of TSP-1 in blood vessels in diabetes may represent a new link between diabetes, atherogenesis, and accelerated restenosis. This increase in TSP-1 production may be a direct response of vascular cells to glucose."}

{"project":"DisGeNET5_gene_disease","denotations":[{"id":"12810612-10#24#29#gene7057","span":{"begin":1468,"end":1473},"obj":"gene7057"},{"id":"12810612-10#50#58#diseaseC0011847","span":{"begin":1494,"end":1502},"obj":"diseaseC0011847"},{"id":"12810612-10#50#58#diseaseC0011849","span":{"begin":1494,"end":1502},"obj":"diseaseC0011849"},{"id":"12810612-10#92#100#diseaseC0011847","span":{"begin":1536,"end":1544},"obj":"diseaseC0011847"},{"id":"12810612-10#92#100#diseaseC0011849","span":{"begin":1536,"end":1544},"obj":"diseaseC0011849"},{"id":"12810612-10#133#143#diseaseC0333186","span":{"begin":1577,"end":1587},"obj":"diseaseC0333186"}],"relations":[{"id":"24#29#gene705750#58#diseaseC0011847","pred":"associated_with","subj":"12810612-10#24#29#gene7057","obj":"12810612-10#50#58#diseaseC0011847"},{"id":"24#29#gene705750#58#diseaseC0011849","pred":"associated_with","subj":"12810612-10#24#29#gene7057","obj":"12810612-10#50#58#diseaseC0011849"},{"id":"24#29#gene705792#100#diseaseC0011847","pred":"associated_with","subj":"12810612-10#24#29#gene7057","obj":"12810612-10#92#100#diseaseC0011847"},{"id":"24#29#gene705792#100#diseaseC0011849","pred":"associated_with","subj":"12810612-10#24#29#gene7057","obj":"12810612-10#92#100#diseaseC0011849"},{"id":"24#29#gene7057133#143#diseaseC0333186","pred":"associated_with","subj":"12810612-10#24#29#gene7057","obj":"12810612-10#133#143#diseaseC0333186"}],"text":"Increased expression of thrombospondin-1 in vessel wall of diabetic Zucker rat.\nBACKGROUND: Thrombospondin-1 (TSP-1) expression in the vascular wall has been related to the development of atherosclerotic lesions and restenosis. TSP-1 promotes the development of neointima and has recently been associated with atherogenesis at a genetic level. Because TSP-1 expression is responsive to glucose stimulation in mesangial cells, we hypothesized that glucose may stimulate its production by vascular cells. Thus, TSP-1 expression in the blood vessel wall may increase, providing a molecular link between diabetes and accelerated vascular lesion development.\nMETHODS AND RESULTS: To determine whether the expression level of TSP-1 in vessel wall is increased in diabetes, aorta and carotid arteries of Zucker rats were used for immunostaining, Western blotting, and in situ RNA hybridization. A significant increase in TSP-1 expression was found in the adventitia of blood vessels from diabetic rats. Consistent with the well-known antiangiogenic effect of TSP-1, the number of vasa vasorum was reduced in aortas from diabetic rats. In cultured endothelial cells, vascular smooth muscle cells, and fibroblasts, TSP-1 expression increased in response to glucose stimulation (\u003e30-fold). After balloon catheter injury to carotid arteries, expression of TSP-1 protein and mRNA was higher at all time points in the vessels of diabetic rats.\nCONCLUSIONS: Increased expression of TSP-1 in blood vessels in diabetes may represent a new link between diabetes, atherogenesis, and accelerated restenosis. This increase in TSP-1 production may be a direct response of vascular cells to glucose."}

{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":509,"end":514},"obj":"gene:7057"},{"id":"T1","span":{"begin":600,"end":608},"obj":"disease:C0011847"},{"id":"T2","span":{"begin":509,"end":514},"obj":"gene:7057"},{"id":"T3","span":{"begin":600,"end":608},"obj":"disease:C0011849"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"text":"Increased expression of thrombospondin-1 in vessel wall of diabetic Zucker rat.\nBACKGROUND: Thrombospondin-1 (TSP-1) expression in the vascular wall has been related to the development of atherosclerotic lesions and restenosis. TSP-1 promotes the development of neointima and has recently been associated with atherogenesis at a genetic level. Because TSP-1 expression is responsive to glucose stimulation in mesangial cells, we hypothesized that glucose may stimulate its production by vascular cells. Thus, TSP-1 expression in the blood vessel wall may increase, providing a molecular link between diabetes and accelerated vascular lesion development.\nMETHODS AND RESULTS: To determine whether the expression level of TSP-1 in vessel wall is increased in diabetes, aorta and carotid arteries of Zucker rats were used for immunostaining, Western blotting, and in situ RNA hybridization. A significant increase in TSP-1 expression was found in the adventitia of blood vessels from diabetic rats. Consistent with the well-known antiangiogenic effect of TSP-1, the number of vasa vasorum was reduced in aortas from diabetic rats. In cultured endothelial cells, vascular smooth muscle cells, and fibroblasts, TSP-1 expression increased in response to glucose stimulation (\u003e30-fold). After balloon catheter injury to carotid arteries, expression of TSP-1 protein and mRNA was higher at all time points in the vessels of diabetic rats.\nCONCLUSIONS: Increased expression of TSP-1 in blood vessels in diabetes may represent a new link between diabetes, atherogenesis, and accelerated restenosis. This increase in TSP-1 production may be a direct response of vascular cells to glucose."}