PubMed:12634321 JSONTXT

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":206},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":207,"end":428},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":429,"end":568},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":569,"end":773},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":774,"end":1049},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":1050,"end":1215},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":206},"obj":"Sentence"},{"id":"T2","span":{"begin":207,"end":428},"obj":"Sentence"},{"id":"T3","span":{"begin":429,"end":568},"obj":"Sentence"},{"id":"T4","span":{"begin":569,"end":773},"obj":"Sentence"},{"id":"T5","span":{"begin":774,"end":1049},"obj":"Sentence"},{"id":"T6","span":{"begin":1050,"end":1215},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":206},"obj":"Sentence"},{"id":"T2","span":{"begin":207,"end":428},"obj":"Sentence"},{"id":"T3","span":{"begin":429,"end":568},"obj":"Sentence"},{"id":"T4","span":{"begin":569,"end":773},"obj":"Sentence"},{"id":"T5","span":{"begin":774,"end":1049},"obj":"Sentence"},{"id":"T6","span":{"begin":1050,"end":1215},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Complex-type biantennary N-glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [beta]-1,4-galactosyltransferase and [beta]-1,2-N-acetylglucosaminyltransferase II.\nA novel recombinant baculovirus expression vector was used to produce His-tagged human transferrin in a transformed insect cell line (Tn5beta4GalT) that constitutively expresses a mammalian beta-1,4-galactosyltransferase. This virus encoded the His-tagged human transferrin protein in conventional fashion under the control of the very late polyhedrin promoter. In addition, to enhance the synthesis of galactosylated biantennary N-glycans, this virus encoded human beta-1,2- N-acetylglucosaminyltransferase II under the control of an immediate-early (ie1) promoter. Detailed analyses by MALDI-TOF MS, exoglycosidase digestion, and two-dimensional HPLC revealed that the N-glycans on the purified recombinant human transferrin produced by this virus-host system included four different fully galactosylated, biantennary, complex-type glycans. Thus, this study describes a novel baculovirus-host system, which can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N-glycans."}

{"project":"GlycoBiology-FMA","denotations":[{"id":"_T1","span":{"begin":96,"end":101},"obj":"FMAID:169002"},{"id":"_T2","span":{"begin":96,"end":101},"obj":"FMAID:68646"},{"id":"_T3","span":{"begin":481,"end":488},"obj":"FMAID:67257"},{"id":"_T4","span":{"begin":481,"end":488},"obj":"FMAID:165447"},{"id":"_T5","span":{"begin":548,"end":558},"obj":"FMAID:165477"},{"id":"_T6","span":{"begin":1153,"end":1165},"obj":"FMAID:62925"},{"id":"_T7","span":{"begin":1153,"end":1165},"obj":"FMAID:167256"}],"namespaces":[{"prefix":"FMAID","uri":"http://purl.org/sig/ont/fma/fma"}],"text":"Complex-type biantennary N-glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [beta]-1,4-galactosyltransferase and [beta]-1,2-N-acetylglucosaminyltransferase II.\nA novel recombinant baculovirus expression vector was used to produce His-tagged human transferrin in a transformed insect cell line (Tn5beta4GalT) that constitutively expresses a mammalian beta-1,4-galactosyltransferase. This virus encoded the His-tagged human transferrin protein in conventional fashion under the control of the very late polyhedrin promoter. In addition, to enhance the synthesis of galactosylated biantennary N-glycans, this virus encoded human beta-1,2- N-acetylglucosaminyltransferase II under the control of an immediate-early (ie1) promoter. Detailed analyses by MALDI-TOF MS, exoglycosidase digestion, and two-dimensional HPLC revealed that the N-glycans on the purified recombinant human transferrin produced by this virus-host system included four different fully galactosylated, biantennary, complex-type glycans. Thus, this study describes a novel baculovirus-host system, which can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N-glycans."}

{"project":"uniprot-human","denotations":[{"id":"T1","span":{"begin":56,"end":67},"obj":"http://www.uniprot.org/uniprot/P02787"},{"id":"T2","span":{"begin":294,"end":305},"obj":"http://www.uniprot.org/uniprot/P02787"},{"id":"T3","span":{"begin":469,"end":480},"obj":"http://www.uniprot.org/uniprot/P02787"},{"id":"T4","span":{"begin":922,"end":933},"obj":"http://www.uniprot.org/uniprot/P02787"},{"id":"T5","span":{"begin":124,"end":155},"obj":"http://www.uniprot.org/uniprot/O60513"},{"id":"T6","span":{"begin":397,"end":427},"obj":"http://www.uniprot.org/uniprot/O60513"},{"id":"T7","span":{"begin":124,"end":155},"obj":"http://www.uniprot.org/uniprot/Q9UBX8"},{"id":"T8","span":{"begin":397,"end":427},"obj":"http://www.uniprot.org/uniprot/Q9UBX8"},{"id":"T9","span":{"begin":124,"end":155},"obj":"http://www.uniprot.org/uniprot/Q5T4Y5"},{"id":"T10","span":{"begin":397,"end":427},"obj":"http://www.uniprot.org/uniprot/Q5T4Y5"},{"id":"T11","span":{"begin":124,"end":155},"obj":"http://www.uniprot.org/uniprot/Q14523"},{"id":"T12","span":{"begin":397,"end":427},"obj":"http://www.uniprot.org/uniprot/Q14523"},{"id":"T13","span":{"begin":124,"end":155},"obj":"http://www.uniprot.org/uniprot/O60512"},{"id":"T14","span":{"begin":397,"end":427},"obj":"http://www.uniprot.org/uniprot/O60512"},{"id":"T15","span":{"begin":124,"end":155},"obj":"http://www.uniprot.org/uniprot/O43286"},{"id":"T16","span":{"begin":397,"end":427},"obj":"http://www.uniprot.org/uniprot/O43286"},{"id":"T17","span":{"begin":124,"end":155},"obj":"http://www.uniprot.org/uniprot/Q9UBV7"},{"id":"T18","span":{"begin":397,"end":427},"obj":"http://www.uniprot.org/uniprot/Q9UBV7"},{"id":"T19","span":{"begin":167,"end":205},"obj":"http://www.uniprot.org/uniprot/Q10469"},{"id":"T20","span":{"begin":678,"end":717},"obj":"http://www.uniprot.org/uniprot/Q10469"},{"id":"T21","span":{"begin":171,"end":202},"obj":"http://www.uniprot.org/uniprot/Q06430"},{"id":"T22","span":{"begin":683,"end":714},"obj":"http://www.uniprot.org/uniprot/Q06430"},{"id":"T23","span":{"begin":171,"end":202},"obj":"http://www.uniprot.org/uniprot/Q8N0V5"},{"id":"T24","span":{"begin":683,"end":714},"obj":"http://www.uniprot.org/uniprot/Q8N0V5"},{"id":"T25","span":{"begin":171,"end":202},"obj":"http://www.uniprot.org/uniprot/Q8NFS9"},{"id":"T26","span":{"begin":683,"end":714},"obj":"http://www.uniprot.org/uniprot/Q8NFS9"},{"id":"T27","span":{"begin":805,"end":807},"obj":"http://www.uniprot.org/uniprot/Q99707"}],"text":"Complex-type biantennary N-glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [beta]-1,4-galactosyltransferase and [beta]-1,2-N-acetylglucosaminyltransferase II.\nA novel recombinant baculovirus expression vector was used to produce His-tagged human transferrin in a transformed insect cell line (Tn5beta4GalT) that constitutively expresses a mammalian beta-1,4-galactosyltransferase. This virus encoded the His-tagged human transferrin protein in conventional fashion under the control of the very late polyhedrin promoter. In addition, to enhance the synthesis of galactosylated biantennary N-glycans, this virus encoded human beta-1,2- N-acetylglucosaminyltransferase II under the control of an immediate-early (ie1) promoter. Detailed analyses by MALDI-TOF MS, exoglycosidase digestion, and two-dimensional HPLC revealed that the N-glycans on the purified recombinant human transferrin produced by this virus-host system included four different fully galactosylated, biantennary, complex-type glycans. Thus, this study describes a novel baculovirus-host system, which can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N-glycans."}

{"project":"uniprot-mouse","denotations":[{"id":"T1","span":{"begin":56,"end":67},"obj":"http://www.uniprot.org/uniprot/Q63915"},{"id":"T2","span":{"begin":294,"end":305},"obj":"http://www.uniprot.org/uniprot/Q63915"},{"id":"T3","span":{"begin":469,"end":480},"obj":"http://www.uniprot.org/uniprot/Q63915"},{"id":"T4","span":{"begin":922,"end":933},"obj":"http://www.uniprot.org/uniprot/Q63915"},{"id":"T5","span":{"begin":86,"end":88},"obj":"http://www.uniprot.org/uniprot/Q76LS9"},{"id":"T6","span":{"begin":134,"end":155},"obj":"http://www.uniprot.org/uniprot/P23336"},{"id":"T7","span":{"begin":406,"end":427},"obj":"http://www.uniprot.org/uniprot/P23336"},{"id":"T8","span":{"begin":171,"end":202},"obj":"http://www.uniprot.org/uniprot/P97402"},{"id":"T9","span":{"begin":683,"end":714},"obj":"http://www.uniprot.org/uniprot/P97402"},{"id":"T10","span":{"begin":805,"end":807},"obj":"http://www.uniprot.org/uniprot/A6H5Y3"}],"text":"Complex-type biantennary N-glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [beta]-1,4-galactosyltransferase and [beta]-1,2-N-acetylglucosaminyltransferase II.\nA novel recombinant baculovirus expression vector was used to produce His-tagged human transferrin in a transformed insect cell line (Tn5beta4GalT) that constitutively expresses a mammalian beta-1,4-galactosyltransferase. This virus encoded the His-tagged human transferrin protein in conventional fashion under the control of the very late polyhedrin promoter. In addition, to enhance the synthesis of galactosylated biantennary N-glycans, this virus encoded human beta-1,2- N-acetylglucosaminyltransferase II under the control of an immediate-early (ie1) promoter. Detailed analyses by MALDI-TOF MS, exoglycosidase digestion, and two-dimensional HPLC revealed that the N-glycans on the purified recombinant human transferrin produced by this virus-host system included four different fully galactosylated, biantennary, complex-type glycans. Thus, this study describes a novel baculovirus-host system, which can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N-glycans."}

{"project":"GlycoBiology-NCBITAXON","denotations":[{"id":"T1","span":{"begin":96,"end":101},"obj":"http://purl.bioontology.org/ontology/STY/T025"},{"id":"T2","span":{"begin":124,"end":128},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/158455"},{"id":"T3","span":{"begin":124,"end":128},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/3554"},{"id":"T4","span":{"begin":161,"end":165},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/3554"},{"id":"T5","span":{"begin":161,"end":165},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/158455"},{"id":"T6","span":{"begin":215,"end":238},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/582816"},{"id":"T7","span":{"begin":227,"end":238},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/558017"},{"id":"T8","span":{"begin":397,"end":401},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/3554"},{"id":"T9","span":{"begin":397,"end":401},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/158455"},{"id":"T10","span":{"begin":434,"end":439},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/184751"},{"id":"T11","span":{"begin":543,"end":547},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/8186"},{"id":"T12","span":{"begin":653,"end":658},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/184751"},{"id":"T13","span":{"begin":673,"end":677},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/158455"},{"id":"T14","span":{"begin":673,"end":677},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/3554"},{"id":"T15","span":{"begin":951,"end":956},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/184751"},{"id":"T16","span":{"begin":1085,"end":1096},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/558017"}],"text":"Complex-type biantennary N-glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [beta]-1,4-galactosyltransferase and [beta]-1,2-N-acetylglucosaminyltransferase II.\nA novel recombinant baculovirus expression vector was used to produce His-tagged human transferrin in a transformed insect cell line (Tn5beta4GalT) that constitutively expresses a mammalian beta-1,4-galactosyltransferase. This virus encoded the His-tagged human transferrin protein in conventional fashion under the control of the very late polyhedrin promoter. In addition, to enhance the synthesis of galactosylated biantennary N-glycans, this virus encoded human beta-1,2- N-acetylglucosaminyltransferase II under the control of an immediate-early (ie1) promoter. Detailed analyses by MALDI-TOF MS, exoglycosidase digestion, and two-dimensional HPLC revealed that the N-glycans on the purified recombinant human transferrin produced by this virus-host system included four different fully galactosylated, biantennary, complex-type glycans. Thus, this study describes a novel baculovirus-host system, which can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N-glycans."}

{"project":"GO-BP","denotations":[{"id":"T1","span":{"begin":124,"end":155},"obj":"http://purl.obolibrary.org/obo/GO_0003945"},{"id":"T2","span":{"begin":397,"end":427},"obj":"http://purl.obolibrary.org/obo/GO_0003945"},{"id":"T3","span":{"begin":171,"end":205},"obj":"http://purl.obolibrary.org/obo/GO_0008455"},{"id":"T4","span":{"begin":683,"end":717},"obj":"http://purl.obolibrary.org/obo/GO_0008455"},{"id":"T5","span":{"begin":597,"end":606},"obj":"http://purl.obolibrary.org/obo/GO_0009058"},{"id":"T6","span":{"begin":824,"end":833},"obj":"http://purl.obolibrary.org/obo/GO_0007586"}],"text":"Complex-type biantennary N-glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [beta]-1,4-galactosyltransferase and [beta]-1,2-N-acetylglucosaminyltransferase II.\nA novel recombinant baculovirus expression vector was used to produce His-tagged human transferrin in a transformed insect cell line (Tn5beta4GalT) that constitutively expresses a mammalian beta-1,4-galactosyltransferase. This virus encoded the His-tagged human transferrin protein in conventional fashion under the control of the very late polyhedrin promoter. In addition, to enhance the synthesis of galactosylated biantennary N-glycans, this virus encoded human beta-1,2- N-acetylglucosaminyltransferase II under the control of an immediate-early (ie1) promoter. Detailed analyses by MALDI-TOF MS, exoglycosidase digestion, and two-dimensional HPLC revealed that the N-glycans on the purified recombinant human transferrin produced by this virus-host system included four different fully galactosylated, biantennary, complex-type glycans. Thus, this study describes a novel baculovirus-host system, which can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N-glycans."}

{"project":"GO-CC","denotations":[{"id":"T1","span":{"begin":96,"end":101},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T2","span":{"begin":330,"end":334},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T3","span":{"begin":957,"end":961},"obj":"http://purl.obolibrary.org/obo/GO_0018995"},{"id":"T4","span":{"begin":1097,"end":1101},"obj":"http://purl.obolibrary.org/obo/GO_0018995"}],"text":"Complex-type biantennary N-glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [beta]-1,4-galactosyltransferase and [beta]-1,2-N-acetylglucosaminyltransferase II.\nA novel recombinant baculovirus expression vector was used to produce His-tagged human transferrin in a transformed insect cell line (Tn5beta4GalT) that constitutively expresses a mammalian beta-1,4-galactosyltransferase. This virus encoded the His-tagged human transferrin protein in conventional fashion under the control of the very late polyhedrin promoter. In addition, to enhance the synthesis of galactosylated biantennary N-glycans, this virus encoded human beta-1,2- N-acetylglucosaminyltransferase II under the control of an immediate-early (ie1) promoter. Detailed analyses by MALDI-TOF MS, exoglycosidase digestion, and two-dimensional HPLC revealed that the N-glycans on the purified recombinant human transferrin produced by this virus-host system included four different fully galactosylated, biantennary, complex-type glycans. Thus, this study describes a novel baculovirus-host system, which can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N-glycans."}

{"project":"bionlp-st-epi-2011-training","denotations":[{"id":"T1","span":{"begin":56,"end":67},"obj":"Protein"},{"id":"T2","span":{"begin":160,"end":205},"obj":"Protein"},{"id":"T3","span":{"begin":294,"end":305},"obj":"Protein"},{"id":"T4","span":{"begin":469,"end":480},"obj":"Protein"},{"id":"T5","span":{"begin":548,"end":558},"obj":"Protein"},{"id":"T6","span":{"begin":673,"end":717},"obj":"Protein"},{"id":"T7","span":{"begin":759,"end":762},"obj":"Protein"},{"id":"T8","span":{"begin":922,"end":933},"obj":"Protein"}],"text":"Complex-type biantennary N-glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [beta]-1,4-galactosyltransferase and [beta]-1,2-N-acetylglucosaminyltransferase II.\nA novel recombinant baculovirus expression vector was used to produce His-tagged human transferrin in a transformed insect cell line (Tn5beta4GalT) that constitutively expresses a mammalian beta-1,4-galactosyltransferase. This virus encoded the His-tagged human transferrin protein in conventional fashion under the control of the very late polyhedrin promoter. In addition, to enhance the synthesis of galactosylated biantennary N-glycans, this virus encoded human beta-1,2- N-acetylglucosaminyltransferase II under the control of an immediate-early (ie1) promoter. Detailed analyses by MALDI-TOF MS, exoglycosidase digestion, and two-dimensional HPLC revealed that the N-glycans on the purified recombinant human transferrin produced by this virus-host system included four different fully galactosylated, biantennary, complex-type glycans. Thus, this study describes a novel baculovirus-host system, which can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N-glycans."}

{"project":"EDAM-topics","denotations":[{"id":"T1","span":{"begin":50,"end":55},"obj":"http://edamontology.org/topic_2815"},{"id":"T2","span":{"begin":288,"end":293},"obj":"http://edamontology.org/topic_2815"},{"id":"T3","span":{"begin":463,"end":468},"obj":"http://edamontology.org/topic_2815"},{"id":"T4","span":{"begin":481,"end":488},"obj":"http://edamontology.org/topic_0078"},{"id":"T5","span":{"begin":559,"end":567},"obj":"http://edamontology.org/topic_1312"},{"id":"T6","span":{"begin":559,"end":567},"obj":"http://edamontology.org/topic_0749"},{"id":"T7","span":{"begin":559,"end":567},"obj":"http://edamontology.org/topic_0111"},{"id":"T8","span":{"begin":667,"end":672},"obj":"http://edamontology.org/topic_2815"},{"id":"T9","span":{"begin":764,"end":772},"obj":"http://edamontology.org/topic_0111"},{"id":"T10","span":{"begin":764,"end":772},"obj":"http://edamontology.org/topic_0749"},{"id":"T11","span":{"begin":764,"end":772},"obj":"http://edamontology.org/topic_1312"},{"id":"T12","span":{"begin":916,"end":921},"obj":"http://edamontology.org/topic_2815"},{"id":"T13","span":{"begin":1061,"end":1066},"obj":"http://edamontology.org/topic_3678"}],"text":"Complex-type biantennary N-glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [beta]-1,4-galactosyltransferase and [beta]-1,2-N-acetylglucosaminyltransferase II.\nA novel recombinant baculovirus expression vector was used to produce His-tagged human transferrin in a transformed insect cell line (Tn5beta4GalT) that constitutively expresses a mammalian beta-1,4-galactosyltransferase. This virus encoded the His-tagged human transferrin protein in conventional fashion under the control of the very late polyhedrin promoter. In addition, to enhance the synthesis of galactosylated biantennary N-glycans, this virus encoded human beta-1,2- N-acetylglucosaminyltransferase II under the control of an immediate-early (ie1) promoter. Detailed analyses by MALDI-TOF MS, exoglycosidase digestion, and two-dimensional HPLC revealed that the N-glycans on the purified recombinant human transferrin produced by this virus-host system included four different fully galactosylated, biantennary, complex-type glycans. Thus, this study describes a novel baculovirus-host system, which can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N-glycans."}

{"project":"EDAM-DFO","denotations":[{"id":"T1","span":{"begin":481,"end":488},"obj":"http://edamontology.org/format_1208"},{"id":"T2","span":{"begin":481,"end":488},"obj":"http://edamontology.org/data_1467"}],"text":"Complex-type biantennary N-glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [beta]-1,4-galactosyltransferase and [beta]-1,2-N-acetylglucosaminyltransferase II.\nA novel recombinant baculovirus expression vector was used to produce His-tagged human transferrin in a transformed insect cell line (Tn5beta4GalT) that constitutively expresses a mammalian beta-1,4-galactosyltransferase. This virus encoded the His-tagged human transferrin protein in conventional fashion under the control of the very late polyhedrin promoter. In addition, to enhance the synthesis of galactosylated biantennary N-glycans, this virus encoded human beta-1,2- N-acetylglucosaminyltransferase II under the control of an immediate-early (ie1) promoter. Detailed analyses by MALDI-TOF MS, exoglycosidase digestion, and two-dimensional HPLC revealed that the N-glycans on the purified recombinant human transferrin produced by this virus-host system included four different fully galactosylated, biantennary, complex-type glycans. Thus, this study describes a novel baculovirus-host system, which can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N-glycans."}

{"project":"GlycoBiology-Motifs","denotations":[{"id":"T1","span":{"begin":25,"end":34},"obj":"http://rdf.glycoinfo.org/glycan/G00027MO"},{"id":"T2","span":{"begin":637,"end":646},"obj":"http://rdf.glycoinfo.org/glycan/G00027MO"},{"id":"T3","span":{"begin":878,"end":887},"obj":"http://rdf.glycoinfo.org/glycan/G00027MO"},{"id":"T4","span":{"begin":1205,"end":1214},"obj":"http://rdf.glycoinfo.org/glycan/G00027MO"}],"text":"Complex-type biantennary N-glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [beta]-1,4-galactosyltransferase and [beta]-1,2-N-acetylglucosaminyltransferase II.\nA novel recombinant baculovirus expression vector was used to produce His-tagged human transferrin in a transformed insect cell line (Tn5beta4GalT) that constitutively expresses a mammalian beta-1,4-galactosyltransferase. This virus encoded the His-tagged human transferrin protein in conventional fashion under the control of the very late polyhedrin promoter. In addition, to enhance the synthesis of galactosylated biantennary N-glycans, this virus encoded human beta-1,2- N-acetylglucosaminyltransferase II under the control of an immediate-early (ie1) promoter. Detailed analyses by MALDI-TOF MS, exoglycosidase digestion, and two-dimensional HPLC revealed that the N-glycans on the purified recombinant human transferrin produced by this virus-host system included four different fully galactosylated, biantennary, complex-type glycans. Thus, this study describes a novel baculovirus-host system, which can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N-glycans."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":50,"end":55},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":73,"end":88},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":288,"end":293},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":463,"end":468},"obj":"OrganismTaxon"},{"id":"T5","span":{"begin":667,"end":672},"obj":"OrganismTaxon"},{"id":"T6","span":{"begin":916,"end":921},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"},{"id":"A2","pred":"db_id","subj":"T2","obj":"7111"},{"id":"A3","pred":"db_id","subj":"T3","obj":"9606"},{"id":"A4","pred":"db_id","subj":"T4","obj":"9606"},{"id":"A5","pred":"db_id","subj":"T5","obj":"9606"},{"id":"A6","pred":"db_id","subj":"T6","obj":"9606"}],"text":"Complex-type biantennary N-glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [beta]-1,4-galactosyltransferase and [beta]-1,2-N-acetylglucosaminyltransferase II.\nA novel recombinant baculovirus expression vector was used to produce His-tagged human transferrin in a transformed insect cell line (Tn5beta4GalT) that constitutively expresses a mammalian beta-1,4-galactosyltransferase. This virus encoded the His-tagged human transferrin protein in conventional fashion under the control of the very late polyhedrin promoter. In addition, to enhance the synthesis of galactosylated biantennary N-glycans, this virus encoded human beta-1,2- N-acetylglucosaminyltransferase II under the control of an immediate-early (ie1) promoter. Detailed analyses by MALDI-TOF MS, exoglycosidase digestion, and two-dimensional HPLC revealed that the N-glycans on the purified recombinant human transferrin produced by this virus-host system included four different fully galactosylated, biantennary, complex-type glycans. Thus, this study describes a novel baculovirus-host system, which can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N-glycans."}