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PubMed:12634321 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-206 Sentence denotes Complex-type biantennary N-glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [beta]-1,4-galactosyltransferase and [beta]-1,2-N-acetylglucosaminyltransferase II.
TextSentencer_T2 207-428 Sentence denotes A novel recombinant baculovirus expression vector was used to produce His-tagged human transferrin in a transformed insect cell line (Tn5beta4GalT) that constitutively expresses a mammalian beta-1,4-galactosyltransferase.
TextSentencer_T3 429-568 Sentence denotes This virus encoded the His-tagged human transferrin protein in conventional fashion under the control of the very late polyhedrin promoter.
TextSentencer_T4 569-773 Sentence denotes In addition, to enhance the synthesis of galactosylated biantennary N-glycans, this virus encoded human beta-1,2- N-acetylglucosaminyltransferase II under the control of an immediate-early (ie1) promoter.
TextSentencer_T5 774-1049 Sentence denotes Detailed analyses by MALDI-TOF MS, exoglycosidase digestion, and two-dimensional HPLC revealed that the N-glycans on the purified recombinant human transferrin produced by this virus-host system included four different fully galactosylated, biantennary, complex-type glycans.
TextSentencer_T6 1050-1215 Sentence denotes Thus, this study describes a novel baculovirus-host system, which can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N-glycans.
T1 0-206 Sentence denotes Complex-type biantennary N-glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [beta]-1,4-galactosyltransferase and [beta]-1,2-N-acetylglucosaminyltransferase II.
T2 207-428 Sentence denotes A novel recombinant baculovirus expression vector was used to produce His-tagged human transferrin in a transformed insect cell line (Tn5beta4GalT) that constitutively expresses a mammalian beta-1,4-galactosyltransferase.
T3 429-568 Sentence denotes This virus encoded the His-tagged human transferrin protein in conventional fashion under the control of the very late polyhedrin promoter.
T4 569-773 Sentence denotes In addition, to enhance the synthesis of galactosylated biantennary N-glycans, this virus encoded human beta-1,2- N-acetylglucosaminyltransferase II under the control of an immediate-early (ie1) promoter.
T5 774-1049 Sentence denotes Detailed analyses by MALDI-TOF MS, exoglycosidase digestion, and two-dimensional HPLC revealed that the N-glycans on the purified recombinant human transferrin produced by this virus-host system included four different fully galactosylated, biantennary, complex-type glycans.
T6 1050-1215 Sentence denotes Thus, this study describes a novel baculovirus-host system, which can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N-glycans.
T1 0-206 Sentence denotes Complex-type biantennary N-glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [beta]-1,4-galactosyltransferase and [beta]-1,2-N-acetylglucosaminyltransferase II.
T2 207-428 Sentence denotes A novel recombinant baculovirus expression vector was used to produce His-tagged human transferrin in a transformed insect cell line (Tn5beta4GalT) that constitutively expresses a mammalian beta-1,4-galactosyltransferase.
T3 429-568 Sentence denotes This virus encoded the His-tagged human transferrin protein in conventional fashion under the control of the very late polyhedrin promoter.
T4 569-773 Sentence denotes In addition, to enhance the synthesis of galactosylated biantennary N-glycans, this virus encoded human beta-1,2- N-acetylglucosaminyltransferase II under the control of an immediate-early (ie1) promoter.
T5 774-1049 Sentence denotes Detailed analyses by MALDI-TOF MS, exoglycosidase digestion, and two-dimensional HPLC revealed that the N-glycans on the purified recombinant human transferrin produced by this virus-host system included four different fully galactosylated, biantennary, complex-type glycans.
T6 1050-1215 Sentence denotes Thus, this study describes a novel baculovirus-host system, which can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N-glycans.

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 96-101 FMAID:169002 denotes cells
_T2 96-101 FMAID:68646 denotes cells
_T3 481-488 FMAID:67257 denotes protein
_T4 481-488 FMAID:165447 denotes protein
_T5 548-558 FMAID:165477 denotes polyhedrin
_T6 1153-1165 FMAID:62925 denotes glycoprotein
_T7 1153-1165 FMAID:167256 denotes glycoprotein

uniprot-human

Id Subject Object Predicate Lexical cue
T1 56-67 http://www.uniprot.org/uniprot/P02787 denotes transferrin
T2 294-305 http://www.uniprot.org/uniprot/P02787 denotes transferrin
T3 469-480 http://www.uniprot.org/uniprot/P02787 denotes transferrin
T4 922-933 http://www.uniprot.org/uniprot/P02787 denotes transferrin
T5 124-155 http://www.uniprot.org/uniprot/O60513 denotes beta]-1,4-galactosyltransferase
T6 397-427 http://www.uniprot.org/uniprot/O60513 denotes beta-1,4-galactosyltransferase
T7 124-155 http://www.uniprot.org/uniprot/Q9UBX8 denotes beta]-1,4-galactosyltransferase
T8 397-427 http://www.uniprot.org/uniprot/Q9UBX8 denotes beta-1,4-galactosyltransferase
T9 124-155 http://www.uniprot.org/uniprot/Q5T4Y5 denotes beta]-1,4-galactosyltransferase
T10 397-427 http://www.uniprot.org/uniprot/Q5T4Y5 denotes beta-1,4-galactosyltransferase
T11 124-155 http://www.uniprot.org/uniprot/Q14523 denotes beta]-1,4-galactosyltransferase
T12 397-427 http://www.uniprot.org/uniprot/Q14523 denotes beta-1,4-galactosyltransferase
T13 124-155 http://www.uniprot.org/uniprot/O60512 denotes beta]-1,4-galactosyltransferase
T14 397-427 http://www.uniprot.org/uniprot/O60512 denotes beta-1,4-galactosyltransferase
T15 124-155 http://www.uniprot.org/uniprot/O43286 denotes beta]-1,4-galactosyltransferase
T16 397-427 http://www.uniprot.org/uniprot/O43286 denotes beta-1,4-galactosyltransferase
T17 124-155 http://www.uniprot.org/uniprot/Q9UBV7 denotes beta]-1,4-galactosyltransferase
T18 397-427 http://www.uniprot.org/uniprot/Q9UBV7 denotes beta-1,4-galactosyltransferase
T19 167-205 http://www.uniprot.org/uniprot/Q10469 denotes 1,2-N-acetylglucosaminyltransferase II
T20 678-717 http://www.uniprot.org/uniprot/Q10469 denotes 1,2- N-acetylglucosaminyltransferase II
T21 171-202 http://www.uniprot.org/uniprot/Q06430 denotes N-acetylglucosaminyltransferase
T22 683-714 http://www.uniprot.org/uniprot/Q06430 denotes N-acetylglucosaminyltransferase
T23 171-202 http://www.uniprot.org/uniprot/Q8N0V5 denotes N-acetylglucosaminyltransferase
T24 683-714 http://www.uniprot.org/uniprot/Q8N0V5 denotes N-acetylglucosaminyltransferase
T25 171-202 http://www.uniprot.org/uniprot/Q8NFS9 denotes N-acetylglucosaminyltransferase
T26 683-714 http://www.uniprot.org/uniprot/Q8NFS9 denotes N-acetylglucosaminyltransferase
T27 805-807 http://www.uniprot.org/uniprot/Q99707 denotes MS

uniprot-mouse

Id Subject Object Predicate Lexical cue
T1 56-67 http://www.uniprot.org/uniprot/Q63915 denotes transferrin
T2 294-305 http://www.uniprot.org/uniprot/Q63915 denotes transferrin
T3 469-480 http://www.uniprot.org/uniprot/Q63915 denotes transferrin
T4 922-933 http://www.uniprot.org/uniprot/Q63915 denotes transferrin
T5 86-88 http://www.uniprot.org/uniprot/Q76LS9 denotes ni
T6 134-155 http://www.uniprot.org/uniprot/P23336 denotes galactosyltransferase
T7 406-427 http://www.uniprot.org/uniprot/P23336 denotes galactosyltransferase
T8 171-202 http://www.uniprot.org/uniprot/P97402 denotes N-acetylglucosaminyltransferase
T9 683-714 http://www.uniprot.org/uniprot/P97402 denotes N-acetylglucosaminyltransferase
T10 805-807 http://www.uniprot.org/uniprot/A6H5Y3 denotes MS

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 96-101 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T2 124-128 http://purl.bioontology.org/ontology/NCBITAXON/158455 denotes beta
T3 124-128 http://purl.bioontology.org/ontology/NCBITAXON/3554 denotes beta
T4 161-165 http://purl.bioontology.org/ontology/NCBITAXON/3554 denotes beta
T5 161-165 http://purl.bioontology.org/ontology/NCBITAXON/158455 denotes beta
T6 215-238 http://purl.bioontology.org/ontology/NCBITAXON/582816 denotes recombinant baculovirus
T7 227-238 http://purl.bioontology.org/ontology/NCBITAXON/558017 denotes baculovirus
T8 397-401 http://purl.bioontology.org/ontology/NCBITAXON/3554 denotes beta
T9 397-401 http://purl.bioontology.org/ontology/NCBITAXON/158455 denotes beta
T10 434-439 http://purl.bioontology.org/ontology/NCBITAXON/184751 denotes virus
T11 543-547 http://purl.bioontology.org/ontology/NCBITAXON/8186 denotes late
T12 653-658 http://purl.bioontology.org/ontology/NCBITAXON/184751 denotes virus
T13 673-677 http://purl.bioontology.org/ontology/NCBITAXON/158455 denotes beta
T14 673-677 http://purl.bioontology.org/ontology/NCBITAXON/3554 denotes beta
T15 951-956 http://purl.bioontology.org/ontology/NCBITAXON/184751 denotes virus
T16 1085-1096 http://purl.bioontology.org/ontology/NCBITAXON/558017 denotes baculovirus

GO-BP

Id Subject Object Predicate Lexical cue
T1 124-155 http://purl.obolibrary.org/obo/GO_0003945 denotes beta]-1,4-galactosyltransferase
T2 397-427 http://purl.obolibrary.org/obo/GO_0003945 denotes beta-1,4-galactosyltransferase
T3 171-205 http://purl.obolibrary.org/obo/GO_0008455 denotes N-acetylglucosaminyltransferase II
T4 683-717 http://purl.obolibrary.org/obo/GO_0008455 denotes N-acetylglucosaminyltransferase II
T5 597-606 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis
T6 824-833 http://purl.obolibrary.org/obo/GO_0007586 denotes digestion

GO-CC

Id Subject Object Predicate Lexical cue
T1 96-101 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2 330-334 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T3 957-961 http://purl.obolibrary.org/obo/GO_0018995 denotes host
T4 1097-1101 http://purl.obolibrary.org/obo/GO_0018995 denotes host

bionlp-st-epi-2011-training

Id Subject Object Predicate Lexical cue
T1 56-67 Protein denotes transferrin
T2 160-205 Protein denotes [beta]-1,2-N-acetylglucosaminyltransferase II
T3 294-305 Protein denotes transferrin
T4 469-480 Protein denotes transferrin
T5 548-558 Protein denotes polyhedrin
T6 673-717 Protein denotes beta-1,2- N-acetylglucosaminyltransferase II
T7 759-762 Protein denotes ie1
T8 922-933 Protein denotes transferrin

EDAM-topics

Id Subject Object Predicate Lexical cue
T1 50-55 http://edamontology.org/topic_2815 denotes human
T2 288-293 http://edamontology.org/topic_2815 denotes human
T3 463-468 http://edamontology.org/topic_2815 denotes human
T4 481-488 http://edamontology.org/topic_0078 denotes protein
T5 559-567 http://edamontology.org/topic_1312 denotes promoter
T6 559-567 http://edamontology.org/topic_0749 denotes promoter
T7 559-567 http://edamontology.org/topic_0111 denotes promoter
T8 667-672 http://edamontology.org/topic_2815 denotes human
T9 764-772 http://edamontology.org/topic_0111 denotes promoter
T10 764-772 http://edamontology.org/topic_0749 denotes promoter
T11 764-772 http://edamontology.org/topic_1312 denotes promoter
T12 916-921 http://edamontology.org/topic_2815 denotes human
T13 1061-1066 http://edamontology.org/topic_3678 denotes study

EDAM-DFO

Id Subject Object Predicate Lexical cue
T1 481-488 http://edamontology.org/format_1208 denotes protein
T2 481-488 http://edamontology.org/data_1467 denotes protein

GlycoBiology-Motifs

Id Subject Object Predicate Lexical cue
T1 25-34 http://rdf.glycoinfo.org/glycan/G00027MO denotes N-glycans
T2 637-646 http://rdf.glycoinfo.org/glycan/G00027MO denotes N-glycans
T3 878-887 http://rdf.glycoinfo.org/glycan/G00027MO denotes N-glycans
T4 1205-1214 http://rdf.glycoinfo.org/glycan/G00027MO denotes N-glycans

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 50-55 OrganismTaxon denotes human 9606
T2 73-88 OrganismTaxon denotes Trichoplusia ni 7111
T3 288-293 OrganismTaxon denotes human 9606
T4 463-468 OrganismTaxon denotes human 9606
T5 667-672 OrganismTaxon denotes human 9606
T6 916-921 OrganismTaxon denotes human 9606