PubMed:12379519 / 0-1011 JSONTXT

{"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T1","span":{"begin":149,"end":324},"obj":"BACKGROUND"},{"id":"T2","span":{"begin":336,"end":586},"obj":"OBJECTIVE"}],"text":"Invasive properties of fibroblast-like synoviocytes: correlation with growth characteristics and expression of MMP-1, MMP-3, and MMP-10.\nBACKGROUND: Matrix metalloproteinases (MMPs) have a pivotal role in the destruction of cartilage in rheumatoid arthritis (RA), which is mediated by the fibroblast-like synoviocytes (FLS).\nOBJECTIVE: To examine the in vitro invasiveness of synoviocytes obtained from inflamed joints of patients with arthritis in relation to the expression of MMP 1-14, 17, 19, cathepsin-K, the tissue inhibitors of matrix metalloproteinases TIMP-1 and TIMP-2 by FLS.\nMETHODS: FLS were derived from 56 patients (30 with RA, 17 with osteoarthritis (OA), and nine with avascular necrosis (AVN)). Invasive growth of FLS through an artificial matrix (Matrigel) was measured in a transwell system. The number of cells that migrated through the matrix were counted. Proliferation rate was determined by counting the FLS after seven days of culturing. Expression of MMPs, cathepsin-K and TIMPs was i"}

{"project":"DisGeNET5_gene_disease","denotations":[{"id":"12379519-2#143#148#gene4312","span":{"begin":479,"end":484},"obj":"gene4312"},{"id":"12379519-2#161#172#gene1513","span":{"begin":497,"end":508},"obj":"gene1513"},{"id":"12379519-2#236#242#gene7077","span":{"begin":572,"end":578},"obj":"gene7077"},{"id":"12379519-2#100#109#diseaseC0003864","span":{"begin":436,"end":445},"obj":"diseaseC0003864"}],"relations":[{"id":"143#148#gene4312100#109#diseaseC0003864","pred":"associated_with","subj":"12379519-2#143#148#gene4312","obj":"12379519-2#100#109#diseaseC0003864"},{"id":"161#172#gene1513100#109#diseaseC0003864","pred":"associated_with","subj":"12379519-2#161#172#gene1513","obj":"12379519-2#100#109#diseaseC0003864"},{"id":"236#242#gene7077100#109#diseaseC0003864","pred":"associated_with","subj":"12379519-2#236#242#gene7077","obj":"12379519-2#100#109#diseaseC0003864"}],"text":"Invasive properties of fibroblast-like synoviocytes: correlation with growth characteristics and expression of MMP-1, MMP-3, and MMP-10.\nBACKGROUND: Matrix metalloproteinases (MMPs) have a pivotal role in the destruction of cartilage in rheumatoid arthritis (RA), which is mediated by the fibroblast-like synoviocytes (FLS).\nOBJECTIVE: To examine the in vitro invasiveness of synoviocytes obtained from inflamed joints of patients with arthritis in relation to the expression of MMP 1-14, 17, 19, cathepsin-K, the tissue inhibitors of matrix metalloproteinases TIMP-1 and TIMP-2 by FLS.\nMETHODS: FLS were derived from 56 patients (30 with RA, 17 with osteoarthritis (OA), and nine with avascular necrosis (AVN)). Invasive growth of FLS through an artificial matrix (Matrigel) was measured in a transwell system. The number of cells that migrated through the matrix were counted. Proliferation rate was determined by counting the FLS after seven days of culturing. Expression of MMPs, cathepsin-K and TIMPs was i"}