PubMed:12122022 JSONTXT

{"project":"Glycan-Motif","denotations":[{"id":"T1","span":{"begin":1044,"end":1051},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"}],"text":"Molecular analysis of a novel family of complex glycoinositolphosphoryl ceramides from Cryptococcus neoformans: structural differences between encapsulated and acapsular yeast forms.\nComplex glycoinositolphosphoryl ceramides (GIPCs) have been purified from a pathogenic encapsulated wild-type (WT) strain of Cryptococcus neoformans var. neoformans and from an acapsular mutant (Cap67). The structures of the GIPCs were determined by a combination of tandem mass spectrometry, nuclear magnetic resonance spectroscopy, methylation analysis, gas chromatography-mass spectrometry, and chemical degradation. The main GIPC from the WT strain had the structure Manp(alpha1-3)[Xylp(beta1-2)] Manp(alpha1-4)Galp(beta1-6)Manp(alpha1-2)Ins-1-phosphoryl ceramide (GIPC A), whereas the compounds from the acapsular mutant were more heterogeneous in their glycan chains, and variants with Manp(alpha1-6) (GIPC B), Manp(alpha1-6) Manp(alpha1-6) (GIPC C), and Manp(alpha1-2)Manp(alpha1-6)Manp(alpha1-6) (GIPC D) substituents linked to the nonreducing terminal mannose residue found in the WT GIPC A were abundant. The ceramide moieties of C. neoformans GIPCs were composed of a C(18) phytosphingosine long-chain base mainly N-acylated with 2-hydroxy-tetracosanoic acid in the WT GIPC while in the acapsular Cap67 mutant GIPCs, as well as 2-hydroxy-tetracosanoic acid, the unusual 2,3-dihydroxy-tetracosanoic acid was characterized. In addition, structural analysis revealed that the amount of GIPC in the WT cells was fourfold less of that in the acapsular mutant."}

{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":1044,"end":1051},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ"}],"text":"Molecular analysis of a novel family of complex glycoinositolphosphoryl ceramides from Cryptococcus neoformans: structural differences between encapsulated and acapsular yeast forms.\nComplex glycoinositolphosphoryl ceramides (GIPCs) have been purified from a pathogenic encapsulated wild-type (WT) strain of Cryptococcus neoformans var. neoformans and from an acapsular mutant (Cap67). The structures of the GIPCs were determined by a combination of tandem mass spectrometry, nuclear magnetic resonance spectroscopy, methylation analysis, gas chromatography-mass spectrometry, and chemical degradation. The main GIPC from the WT strain had the structure Manp(alpha1-3)[Xylp(beta1-2)] Manp(alpha1-4)Galp(beta1-6)Manp(alpha1-2)Ins-1-phosphoryl ceramide (GIPC A), whereas the compounds from the acapsular mutant were more heterogeneous in their glycan chains, and variants with Manp(alpha1-6) (GIPC B), Manp(alpha1-6) Manp(alpha1-6) (GIPC C), and Manp(alpha1-2)Manp(alpha1-6)Manp(alpha1-6) (GIPC D) substituents linked to the nonreducing terminal mannose residue found in the WT GIPC A were abundant. The ceramide moieties of C. neoformans GIPCs were composed of a C(18) phytosphingosine long-chain base mainly N-acylated with 2-hydroxy-tetracosanoic acid in the WT GIPC while in the acapsular Cap67 mutant GIPCs, as well as 2-hydroxy-tetracosanoic acid, the unusual 2,3-dihydroxy-tetracosanoic acid was characterized. In addition, structural analysis revealed that the amount of GIPC in the WT cells was fourfold less of that in the acapsular mutant."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":182},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":183,"end":385},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":386,"end":602},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":603,"end":1097},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":1098,"end":1415},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":1416,"end":1548},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":182},"obj":"Sentence"},{"id":"T2","span":{"begin":183,"end":385},"obj":"Sentence"},{"id":"T3","span":{"begin":386,"end":602},"obj":"Sentence"},{"id":"T4","span":{"begin":603,"end":1097},"obj":"Sentence"},{"id":"T5","span":{"begin":1098,"end":1415},"obj":"Sentence"},{"id":"T6","span":{"begin":1416,"end":1548},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":182},"obj":"Sentence"},{"id":"T2","span":{"begin":183,"end":385},"obj":"Sentence"},{"id":"T3","span":{"begin":386,"end":602},"obj":"Sentence"},{"id":"T4","span":{"begin":603,"end":1097},"obj":"Sentence"},{"id":"T5","span":{"begin":1098,"end":1415},"obj":"Sentence"},{"id":"T6","span":{"begin":1416,"end":1548},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Molecular analysis of a novel family of complex glycoinositolphosphoryl ceramides from Cryptococcus neoformans: structural differences between encapsulated and acapsular yeast forms.\nComplex glycoinositolphosphoryl ceramides (GIPCs) have been purified from a pathogenic encapsulated wild-type (WT) strain of Cryptococcus neoformans var. neoformans and from an acapsular mutant (Cap67). The structures of the GIPCs were determined by a combination of tandem mass spectrometry, nuclear magnetic resonance spectroscopy, methylation analysis, gas chromatography-mass spectrometry, and chemical degradation. The main GIPC from the WT strain had the structure Manp(alpha1-3)[Xylp(beta1-2)] Manp(alpha1-4)Galp(beta1-6)Manp(alpha1-2)Ins-1-phosphoryl ceramide (GIPC A), whereas the compounds from the acapsular mutant were more heterogeneous in their glycan chains, and variants with Manp(alpha1-6) (GIPC B), Manp(alpha1-6) Manp(alpha1-6) (GIPC C), and Manp(alpha1-2)Manp(alpha1-6)Manp(alpha1-6) (GIPC D) substituents linked to the nonreducing terminal mannose residue found in the WT GIPC A were abundant. The ceramide moieties of C. neoformans GIPCs were composed of a C(18) phytosphingosine long-chain base mainly N-acylated with 2-hydroxy-tetracosanoic acid in the WT GIPC while in the acapsular Cap67 mutant GIPCs, as well as 2-hydroxy-tetracosanoic acid, the unusual 2,3-dihydroxy-tetracosanoic acid was characterized. In addition, structural analysis revealed that the amount of GIPC in the WT cells was fourfold less of that in the acapsular mutant."}

{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":1044,"end":1051},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"}],"text":"Molecular analysis of a novel family of complex glycoinositolphosphoryl ceramides from Cryptococcus neoformans: structural differences between encapsulated and acapsular yeast forms.\nComplex glycoinositolphosphoryl ceramides (GIPCs) have been purified from a pathogenic encapsulated wild-type (WT) strain of Cryptococcus neoformans var. neoformans and from an acapsular mutant (Cap67). The structures of the GIPCs were determined by a combination of tandem mass spectrometry, nuclear magnetic resonance spectroscopy, methylation analysis, gas chromatography-mass spectrometry, and chemical degradation. The main GIPC from the WT strain had the structure Manp(alpha1-3)[Xylp(beta1-2)] Manp(alpha1-4)Galp(beta1-6)Manp(alpha1-2)Ins-1-phosphoryl ceramide (GIPC A), whereas the compounds from the acapsular mutant were more heterogeneous in their glycan chains, and variants with Manp(alpha1-6) (GIPC B), Manp(alpha1-6) Manp(alpha1-6) (GIPC C), and Manp(alpha1-2)Manp(alpha1-6)Manp(alpha1-6) (GIPC D) substituents linked to the nonreducing terminal mannose residue found in the WT GIPC A were abundant. The ceramide moieties of C. neoformans GIPCs were composed of a C(18) phytosphingosine long-chain base mainly N-acylated with 2-hydroxy-tetracosanoic acid in the WT GIPC while in the acapsular Cap67 mutant GIPCs, as well as 2-hydroxy-tetracosanoic acid, the unusual 2,3-dihydroxy-tetracosanoic acid was characterized. In addition, structural analysis revealed that the amount of GIPC in the WT cells was fourfold less of that in the acapsular mutant."}

{"project":"GlycoBiology-PACDB","denotations":[{"id":"_T1","span":{"begin":87,"end":110},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC642"},{"id":"_T2","span":{"begin":87,"end":110},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC759"},{"id":"_T3","span":{"begin":87,"end":110},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC597"},{"id":"_T4","span":{"begin":308,"end":331},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC642"},{"id":"_T5","span":{"begin":308,"end":331},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC759"},{"id":"_T6","span":{"begin":308,"end":331},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC597"},{"id":"_T7","span":{"begin":308,"end":347},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC074"},{"id":"_T8","span":{"begin":308,"end":347},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC067"},{"id":"_T9","span":{"begin":308,"end":347},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC753"}],"text":"Molecular analysis of a novel family of complex glycoinositolphosphoryl ceramides from Cryptococcus neoformans: structural differences between encapsulated and acapsular yeast forms.\nComplex glycoinositolphosphoryl ceramides (GIPCs) have been purified from a pathogenic encapsulated wild-type (WT) strain of Cryptococcus neoformans var. neoformans and from an acapsular mutant (Cap67). The structures of the GIPCs were determined by a combination of tandem mass spectrometry, nuclear magnetic resonance spectroscopy, methylation analysis, gas chromatography-mass spectrometry, and chemical degradation. The main GIPC from the WT strain had the structure Manp(alpha1-3)[Xylp(beta1-2)] Manp(alpha1-4)Galp(beta1-6)Manp(alpha1-2)Ins-1-phosphoryl ceramide (GIPC A), whereas the compounds from the acapsular mutant were more heterogeneous in their glycan chains, and variants with Manp(alpha1-6) (GIPC B), Manp(alpha1-6) Manp(alpha1-6) (GIPC C), and Manp(alpha1-2)Manp(alpha1-6)Manp(alpha1-6) (GIPC D) substituents linked to the nonreducing terminal mannose residue found in the WT GIPC A were abundant. The ceramide moieties of C. neoformans GIPCs were composed of a C(18) phytosphingosine long-chain base mainly N-acylated with 2-hydroxy-tetracosanoic acid in the WT GIPC while in the acapsular Cap67 mutant GIPCs, as well as 2-hydroxy-tetracosanoic acid, the unusual 2,3-dihydroxy-tetracosanoic acid was characterized. In addition, structural analysis revealed that the amount of GIPC in the WT cells was fourfold less of that in the acapsular mutant."}

{"project":"GlycoBiology-FMA","denotations":[{"id":"_T1","span":{"begin":539,"end":542},"obj":"FMAID:85816"},{"id":"_T2","span":{"begin":607,"end":611},"obj":"FMAID:214734"},{"id":"_T3","span":{"begin":773,"end":782},"obj":"FMAID:67745"},{"id":"_T4","span":{"begin":773,"end":782},"obj":"FMAID:165656"},{"id":"_T5","span":{"begin":1044,"end":1051},"obj":"FMAID:82801"},{"id":"_T6","span":{"begin":1044,"end":1051},"obj":"FMAID:196796"},{"id":"_T7","span":{"begin":1492,"end":1497},"obj":"FMAID:68646"},{"id":"_T8","span":{"begin":1492,"end":1497},"obj":"FMAID:169002"}],"namespaces":[{"prefix":"FMAID","uri":"http://purl.org/sig/ont/fma/fma"}],"text":"Molecular analysis of a novel family of complex glycoinositolphosphoryl ceramides from Cryptococcus neoformans: structural differences between encapsulated and acapsular yeast forms.\nComplex glycoinositolphosphoryl ceramides (GIPCs) have been purified from a pathogenic encapsulated wild-type (WT) strain of Cryptococcus neoformans var. neoformans and from an acapsular mutant (Cap67). The structures of the GIPCs were determined by a combination of tandem mass spectrometry, nuclear magnetic resonance spectroscopy, methylation analysis, gas chromatography-mass spectrometry, and chemical degradation. The main GIPC from the WT strain had the structure Manp(alpha1-3)[Xylp(beta1-2)] Manp(alpha1-4)Galp(beta1-6)Manp(alpha1-2)Ins-1-phosphoryl ceramide (GIPC A), whereas the compounds from the acapsular mutant were more heterogeneous in their glycan chains, and variants with Manp(alpha1-6) (GIPC B), Manp(alpha1-6) Manp(alpha1-6) (GIPC C), and Manp(alpha1-2)Manp(alpha1-6)Manp(alpha1-6) (GIPC D) substituents linked to the nonreducing terminal mannose residue found in the WT GIPC A were abundant. The ceramide moieties of C. neoformans GIPCs were composed of a C(18) phytosphingosine long-chain base mainly N-acylated with 2-hydroxy-tetracosanoic acid in the WT GIPC while in the acapsular Cap67 mutant GIPCs, as well as 2-hydroxy-tetracosanoic acid, the unusual 2,3-dihydroxy-tetracosanoic acid was characterized. In addition, structural analysis revealed that the amount of GIPC in the WT cells was fourfold less of that in the acapsular mutant."}

{"project":"uniprot-human","denotations":[{"id":"T1","span":{"begin":612,"end":616},"obj":"http://www.uniprot.org/uniprot/O14908"},{"id":"T2","span":{"begin":752,"end":756},"obj":"http://www.uniprot.org/uniprot/O14908"},{"id":"T3","span":{"begin":891,"end":895},"obj":"http://www.uniprot.org/uniprot/O14908"},{"id":"T4","span":{"begin":931,"end":935},"obj":"http://www.uniprot.org/uniprot/O14908"},{"id":"T5","span":{"begin":988,"end":992},"obj":"http://www.uniprot.org/uniprot/O14908"},{"id":"T6","span":{"begin":1076,"end":1080},"obj":"http://www.uniprot.org/uniprot/O14908"},{"id":"T7","span":{"begin":1263,"end":1267},"obj":"http://www.uniprot.org/uniprot/O14908"},{"id":"T8","span":{"begin":1477,"end":1481},"obj":"http://www.uniprot.org/uniprot/O14908"}],"text":"Molecular analysis of a novel family of complex glycoinositolphosphoryl ceramides from Cryptococcus neoformans: structural differences between encapsulated and acapsular yeast forms.\nComplex glycoinositolphosphoryl ceramides (GIPCs) have been purified from a pathogenic encapsulated wild-type (WT) strain of Cryptococcus neoformans var. neoformans and from an acapsular mutant (Cap67). The structures of the GIPCs were determined by a combination of tandem mass spectrometry, nuclear magnetic resonance spectroscopy, methylation analysis, gas chromatography-mass spectrometry, and chemical degradation. The main GIPC from the WT strain had the structure Manp(alpha1-3)[Xylp(beta1-2)] Manp(alpha1-4)Galp(beta1-6)Manp(alpha1-2)Ins-1-phosphoryl ceramide (GIPC A), whereas the compounds from the acapsular mutant were more heterogeneous in their glycan chains, and variants with Manp(alpha1-6) (GIPC B), Manp(alpha1-6) Manp(alpha1-6) (GIPC C), and Manp(alpha1-2)Manp(alpha1-6)Manp(alpha1-6) (GIPC D) substituents linked to the nonreducing terminal mannose residue found in the WT GIPC A were abundant. The ceramide moieties of C. neoformans GIPCs were composed of a C(18) phytosphingosine long-chain base mainly N-acylated with 2-hydroxy-tetracosanoic acid in the WT GIPC while in the acapsular Cap67 mutant GIPCs, as well as 2-hydroxy-tetracosanoic acid, the unusual 2,3-dihydroxy-tetracosanoic acid was characterized. In addition, structural analysis revealed that the amount of GIPC in the WT cells was fourfold less of that in the acapsular mutant."}

{"project":"uniprot-mouse","denotations":[{"id":"T1","span":{"begin":332,"end":335},"obj":"http://www.uniprot.org/uniprot/Q9Z1Q9"},{"id":"T2","span":{"begin":654,"end":658},"obj":"http://www.uniprot.org/uniprot/Q00PI9"},{"id":"T3","span":{"begin":684,"end":688},"obj":"http://www.uniprot.org/uniprot/Q00PI9"},{"id":"T4","span":{"begin":711,"end":715},"obj":"http://www.uniprot.org/uniprot/Q00PI9"},{"id":"T5","span":{"begin":875,"end":879},"obj":"http://www.uniprot.org/uniprot/Q00PI9"},{"id":"T6","span":{"begin":900,"end":904},"obj":"http://www.uniprot.org/uniprot/Q00PI9"},{"id":"T7","span":{"begin":915,"end":919},"obj":"http://www.uniprot.org/uniprot/Q00PI9"},{"id":"T8","span":{"begin":944,"end":948},"obj":"http://www.uniprot.org/uniprot/Q00PI9"},{"id":"T9","span":{"begin":958,"end":962},"obj":"http://www.uniprot.org/uniprot/Q00PI9"},{"id":"T10","span":{"begin":972,"end":976},"obj":"http://www.uniprot.org/uniprot/Q00PI9"}],"text":"Molecular analysis of a novel family of complex glycoinositolphosphoryl ceramides from Cryptococcus neoformans: structural differences between encapsulated and acapsular yeast forms.\nComplex glycoinositolphosphoryl ceramides (GIPCs) have been purified from a pathogenic encapsulated wild-type (WT) strain of Cryptococcus neoformans var. neoformans and from an acapsular mutant (Cap67). The structures of the GIPCs were determined by a combination of tandem mass spectrometry, nuclear magnetic resonance spectroscopy, methylation analysis, gas chromatography-mass spectrometry, and chemical degradation. The main GIPC from the WT strain had the structure Manp(alpha1-3)[Xylp(beta1-2)] Manp(alpha1-4)Galp(beta1-6)Manp(alpha1-2)Ins-1-phosphoryl ceramide (GIPC A), whereas the compounds from the acapsular mutant were more heterogeneous in their glycan chains, and variants with Manp(alpha1-6) (GIPC B), Manp(alpha1-6) Manp(alpha1-6) (GIPC C), and Manp(alpha1-2)Manp(alpha1-6)Manp(alpha1-6) (GIPC D) substituents linked to the nonreducing terminal mannose residue found in the WT GIPC A were abundant. The ceramide moieties of C. neoformans GIPCs were composed of a C(18) phytosphingosine long-chain base mainly N-acylated with 2-hydroxy-tetracosanoic acid in the WT GIPC while in the acapsular Cap67 mutant GIPCs, as well as 2-hydroxy-tetracosanoic acid, the unusual 2,3-dihydroxy-tetracosanoic acid was characterized. In addition, structural analysis revealed that the amount of GIPC in the WT cells was fourfold less of that in the acapsular mutant."}

{"project":"GlycoBiology-NCBITAXON","denotations":[{"id":"T1","span":{"begin":87,"end":99},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/89915"},{"id":"T2","span":{"begin":87,"end":99},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/107441"},{"id":"T3","span":{"begin":87,"end":99},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/106841"},{"id":"T4","span":{"begin":87,"end":99},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/561281"},{"id":"T5","span":{"begin":87,"end":99},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/104409"},{"id":"T6","span":{"begin":87,"end":99},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/214992"},{"id":"T7","span":{"begin":308,"end":320},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/561281"},{"id":"T8","span":{"begin":308,"end":320},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/104409"},{"id":"T9","span":{"begin":308,"end":320},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/89915"},{"id":"T10","span":{"begin":308,"end":320},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/106841"},{"id":"T11","span":{"begin":308,"end":320},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/107441"},{"id":"T12","span":{"begin":308,"end":320},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/214992"},{"id":"T13","span":{"begin":819,"end":832},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/1113441"},{"id":"T14","span":{"begin":819,"end":832},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/691256"},{"id":"T15","span":{"begin":1492,"end":1497},"obj":"http://purl.bioontology.org/ontology/STY/T025"}],"text":"Molecular analysis of a novel family of complex glycoinositolphosphoryl ceramides from Cryptococcus neoformans: structural differences between encapsulated and acapsular yeast forms.\nComplex glycoinositolphosphoryl ceramides (GIPCs) have been purified from a pathogenic encapsulated wild-type (WT) strain of Cryptococcus neoformans var. neoformans and from an acapsular mutant (Cap67). The structures of the GIPCs were determined by a combination of tandem mass spectrometry, nuclear magnetic resonance spectroscopy, methylation analysis, gas chromatography-mass spectrometry, and chemical degradation. The main GIPC from the WT strain had the structure Manp(alpha1-3)[Xylp(beta1-2)] Manp(alpha1-4)Galp(beta1-6)Manp(alpha1-2)Ins-1-phosphoryl ceramide (GIPC A), whereas the compounds from the acapsular mutant were more heterogeneous in their glycan chains, and variants with Manp(alpha1-6) (GIPC B), Manp(alpha1-6) Manp(alpha1-6) (GIPC C), and Manp(alpha1-2)Manp(alpha1-6)Manp(alpha1-6) (GIPC D) substituents linked to the nonreducing terminal mannose residue found in the WT GIPC A were abundant. The ceramide moieties of C. neoformans GIPCs were composed of a C(18) phytosphingosine long-chain base mainly N-acylated with 2-hydroxy-tetracosanoic acid in the WT GIPC while in the acapsular Cap67 mutant GIPCs, as well as 2-hydroxy-tetracosanoic acid, the unusual 2,3-dihydroxy-tetracosanoic acid was characterized. In addition, structural analysis revealed that the amount of GIPC in the WT cells was fourfold less of that in the acapsular mutant."}

{"project":"GO-BP","denotations":[{"id":"T1","span":{"begin":517,"end":528},"obj":"http://purl.obolibrary.org/obo/GO_0032259"},{"id":"T2","span":{"begin":539,"end":542},"obj":"http://purl.obolibrary.org/obo/GO_0034005"},{"id":"T3","span":{"begin":590,"end":601},"obj":"http://purl.obolibrary.org/obo/GO_0009056"},{"id":"T4","span":{"begin":731,"end":741},"obj":"http://purl.obolibrary.org/obo/GO_0016310"}],"text":"Molecular analysis of a novel family of complex glycoinositolphosphoryl ceramides from Cryptococcus neoformans: structural differences between encapsulated and acapsular yeast forms.\nComplex glycoinositolphosphoryl ceramides (GIPCs) have been purified from a pathogenic encapsulated wild-type (WT) strain of Cryptococcus neoformans var. neoformans and from an acapsular mutant (Cap67). The structures of the GIPCs were determined by a combination of tandem mass spectrometry, nuclear magnetic resonance spectroscopy, methylation analysis, gas chromatography-mass spectrometry, and chemical degradation. The main GIPC from the WT strain had the structure Manp(alpha1-3)[Xylp(beta1-2)] Manp(alpha1-4)Galp(beta1-6)Manp(alpha1-2)Ins-1-phosphoryl ceramide (GIPC A), whereas the compounds from the acapsular mutant were more heterogeneous in their glycan chains, and variants with Manp(alpha1-6) (GIPC B), Manp(alpha1-6) Manp(alpha1-6) (GIPC C), and Manp(alpha1-2)Manp(alpha1-6)Manp(alpha1-6) (GIPC D) substituents linked to the nonreducing terminal mannose residue found in the WT GIPC A were abundant. The ceramide moieties of C. neoformans GIPCs were composed of a C(18) phytosphingosine long-chain base mainly N-acylated with 2-hydroxy-tetracosanoic acid in the WT GIPC while in the acapsular Cap67 mutant GIPCs, as well as 2-hydroxy-tetracosanoic acid, the unusual 2,3-dihydroxy-tetracosanoic acid was characterized. In addition, structural analysis revealed that the amount of GIPC in the WT cells was fourfold less of that in the acapsular mutant."}

{"project":"GO-CC","denotations":[{"id":"T1","span":{"begin":1492,"end":1497},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Molecular analysis of a novel family of complex glycoinositolphosphoryl ceramides from Cryptococcus neoformans: structural differences between encapsulated and acapsular yeast forms.\nComplex glycoinositolphosphoryl ceramides (GIPCs) have been purified from a pathogenic encapsulated wild-type (WT) strain of Cryptococcus neoformans var. neoformans and from an acapsular mutant (Cap67). The structures of the GIPCs were determined by a combination of tandem mass spectrometry, nuclear magnetic resonance spectroscopy, methylation analysis, gas chromatography-mass spectrometry, and chemical degradation. The main GIPC from the WT strain had the structure Manp(alpha1-3)[Xylp(beta1-2)] Manp(alpha1-4)Galp(beta1-6)Manp(alpha1-2)Ins-1-phosphoryl ceramide (GIPC A), whereas the compounds from the acapsular mutant were more heterogeneous in their glycan chains, and variants with Manp(alpha1-6) (GIPC B), Manp(alpha1-6) Manp(alpha1-6) (GIPC C), and Manp(alpha1-2)Manp(alpha1-6)Manp(alpha1-6) (GIPC D) substituents linked to the nonreducing terminal mannose residue found in the WT GIPC A were abundant. The ceramide moieties of C. neoformans GIPCs were composed of a C(18) phytosphingosine long-chain base mainly N-acylated with 2-hydroxy-tetracosanoic acid in the WT GIPC while in the acapsular Cap67 mutant GIPCs, as well as 2-hydroxy-tetracosanoic acid, the unusual 2,3-dihydroxy-tetracosanoic acid was characterized. In addition, structural analysis revealed that the amount of GIPC in the WT cells was fourfold less of that in the acapsular mutant."}

{"project":"EDAM-topics","denotations":[{"id":"T1","span":{"begin":170,"end":175},"obj":"http://edamontology.org/topic_2817"},{"id":"T2","span":{"begin":170,"end":175},"obj":"http://edamontology.org/topic_0782"},{"id":"T3","span":{"begin":259,"end":269},"obj":"http://edamontology.org/topic_0783"},{"id":"T4","span":{"begin":457,"end":474},"obj":"http://edamontology.org/topic_0134"},{"id":"T5","span":{"begin":457,"end":474},"obj":"http://edamontology.org/topic_3520"},{"id":"T6","span":{"begin":476,"end":502},"obj":"http://edamontology.org/topic_3444"},{"id":"T7","span":{"begin":476,"end":515},"obj":"http://edamontology.org/topic_0593"},{"id":"T8","span":{"begin":503,"end":515},"obj":"http://edamontology.org/topic_0593"},{"id":"T9","span":{"begin":558,"end":575},"obj":"http://edamontology.org/topic_0134"},{"id":"T10","span":{"begin":558,"end":575},"obj":"http://edamontology.org/topic_3520"},{"id":"T11","span":{"begin":1035,"end":1043},"obj":"http://edamontology.org/topic_0749"},{"id":"T12","span":{"begin":1429,"end":1448},"obj":"http://edamontology.org/topic_0081"}],"text":"Molecular analysis of a novel family of complex glycoinositolphosphoryl ceramides from Cryptococcus neoformans: structural differences between encapsulated and acapsular yeast forms.\nComplex glycoinositolphosphoryl ceramides (GIPCs) have been purified from a pathogenic encapsulated wild-type (WT) strain of Cryptococcus neoformans var. neoformans and from an acapsular mutant (Cap67). The structures of the GIPCs were determined by a combination of tandem mass spectrometry, nuclear magnetic resonance spectroscopy, methylation analysis, gas chromatography-mass spectrometry, and chemical degradation. The main GIPC from the WT strain had the structure Manp(alpha1-3)[Xylp(beta1-2)] Manp(alpha1-4)Galp(beta1-6)Manp(alpha1-2)Ins-1-phosphoryl ceramide (GIPC A), whereas the compounds from the acapsular mutant were more heterogeneous in their glycan chains, and variants with Manp(alpha1-6) (GIPC B), Manp(alpha1-6) Manp(alpha1-6) (GIPC C), and Manp(alpha1-2)Manp(alpha1-6)Manp(alpha1-6) (GIPC D) substituents linked to the nonreducing terminal mannose residue found in the WT GIPC A were abundant. The ceramide moieties of C. neoformans GIPCs were composed of a C(18) phytosphingosine long-chain base mainly N-acylated with 2-hydroxy-tetracosanoic acid in the WT GIPC while in the acapsular Cap67 mutant GIPCs, as well as 2-hydroxy-tetracosanoic acid, the unusual 2,3-dihydroxy-tetracosanoic acid was characterized. In addition, structural analysis revealed that the amount of GIPC in the WT cells was fourfold less of that in the acapsular mutant."}

{"project":"EDAM-DFO","denotations":[{"id":"T1","span":{"begin":0,"end":18},"obj":"http://edamontology.org/operation_2948"},{"id":"T2","span":{"begin":0,"end":18},"obj":"http://edamontology.org/operation_3351"},{"id":"T3","span":{"begin":10,"end":18},"obj":"http://edamontology.org/operation_2945"},{"id":"T4","span":{"begin":112,"end":122},"obj":"http://edamontology.org/data_0883"},{"id":"T5","span":{"begin":259,"end":269},"obj":"http://edamontology.org/data_3718"},{"id":"T6","span":{"begin":390,"end":400},"obj":"http://edamontology.org/data_0883"},{"id":"T7","span":{"begin":457,"end":474},"obj":"http://edamontology.org/data_2536"},{"id":"T8","span":{"begin":457,"end":474},"obj":"http://edamontology.org/data_3147"},{"id":"T9","span":{"begin":517,"end":537},"obj":"http://edamontology.org/operation_3204"},{"id":"T10","span":{"begin":529,"end":537},"obj":"http://edamontology.org/operation_2945"},{"id":"T11","span":{"begin":558,"end":575},"obj":"http://edamontology.org/data_3147"},{"id":"T12","span":{"begin":558,"end":575},"obj":"http://edamontology.org/data_2536"},{"id":"T13","span":{"begin":644,"end":653},"obj":"http://edamontology.org/data_0883"},{"id":"T14","span":{"begin":1052,"end":1059},"obj":"http://edamontology.org/data_1756"},{"id":"T15","span":{"begin":1429,"end":1439},"obj":"http://edamontology.org/data_0883"},{"id":"T16","span":{"begin":1429,"end":1448},"obj":"http://edamontology.org/operation_2480"},{"id":"T17","span":{"begin":1440,"end":1448},"obj":"http://edamontology.org/operation_2945"}],"text":"Molecular analysis of a novel family of complex glycoinositolphosphoryl ceramides from Cryptococcus neoformans: structural differences between encapsulated and acapsular yeast forms.\nComplex glycoinositolphosphoryl ceramides (GIPCs) have been purified from a pathogenic encapsulated wild-type (WT) strain of Cryptococcus neoformans var. neoformans and from an acapsular mutant (Cap67). The structures of the GIPCs were determined by a combination of tandem mass spectrometry, nuclear magnetic resonance spectroscopy, methylation analysis, gas chromatography-mass spectrometry, and chemical degradation. The main GIPC from the WT strain had the structure Manp(alpha1-3)[Xylp(beta1-2)] Manp(alpha1-4)Galp(beta1-6)Manp(alpha1-2)Ins-1-phosphoryl ceramide (GIPC A), whereas the compounds from the acapsular mutant were more heterogeneous in their glycan chains, and variants with Manp(alpha1-6) (GIPC B), Manp(alpha1-6) Manp(alpha1-6) (GIPC C), and Manp(alpha1-2)Manp(alpha1-6)Manp(alpha1-6) (GIPC D) substituents linked to the nonreducing terminal mannose residue found in the WT GIPC A were abundant. The ceramide moieties of C. neoformans GIPCs were composed of a C(18) phytosphingosine long-chain base mainly N-acylated with 2-hydroxy-tetracosanoic acid in the WT GIPC while in the acapsular Cap67 mutant GIPCs, as well as 2-hydroxy-tetracosanoic acid, the unusual 2,3-dihydroxy-tetracosanoic acid was characterized. In addition, structural analysis revealed that the amount of GIPC in the WT cells was fourfold less of that in the acapsular mutant."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":87,"end":110},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":308,"end":347},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"5207"},{"id":"A2","pred":"db_id","subj":"T2","obj":"40410"}],"text":"Molecular analysis of a novel family of complex glycoinositolphosphoryl ceramides from Cryptococcus neoformans: structural differences between encapsulated and acapsular yeast forms.\nComplex glycoinositolphosphoryl ceramides (GIPCs) have been purified from a pathogenic encapsulated wild-type (WT) strain of Cryptococcus neoformans var. neoformans and from an acapsular mutant (Cap67). The structures of the GIPCs were determined by a combination of tandem mass spectrometry, nuclear magnetic resonance spectroscopy, methylation analysis, gas chromatography-mass spectrometry, and chemical degradation. The main GIPC from the WT strain had the structure Manp(alpha1-3)[Xylp(beta1-2)] Manp(alpha1-4)Galp(beta1-6)Manp(alpha1-2)Ins-1-phosphoryl ceramide (GIPC A), whereas the compounds from the acapsular mutant were more heterogeneous in their glycan chains, and variants with Manp(alpha1-6) (GIPC B), Manp(alpha1-6) Manp(alpha1-6) (GIPC C), and Manp(alpha1-2)Manp(alpha1-6)Manp(alpha1-6) (GIPC D) substituents linked to the nonreducing terminal mannose residue found in the WT GIPC A were abundant. The ceramide moieties of C. neoformans GIPCs were composed of a C(18) phytosphingosine long-chain base mainly N-acylated with 2-hydroxy-tetracosanoic acid in the WT GIPC while in the acapsular Cap67 mutant GIPCs, as well as 2-hydroxy-tetracosanoic acid, the unusual 2,3-dihydroxy-tetracosanoic acid was characterized. In addition, structural analysis revealed that the amount of GIPC in the WT cells was fourfold less of that in the acapsular mutant."}

{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":170,"end":181},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000334"}],"text":"Molecular analysis of a novel family of complex glycoinositolphosphoryl ceramides from Cryptococcus neoformans: structural differences between encapsulated and acapsular yeast forms.\nComplex glycoinositolphosphoryl ceramides (GIPCs) have been purified from a pathogenic encapsulated wild-type (WT) strain of Cryptococcus neoformans var. neoformans and from an acapsular mutant (Cap67). The structures of the GIPCs were determined by a combination of tandem mass spectrometry, nuclear magnetic resonance spectroscopy, methylation analysis, gas chromatography-mass spectrometry, and chemical degradation. The main GIPC from the WT strain had the structure Manp(alpha1-3)[Xylp(beta1-2)] Manp(alpha1-4)Galp(beta1-6)Manp(alpha1-2)Ins-1-phosphoryl ceramide (GIPC A), whereas the compounds from the acapsular mutant were more heterogeneous in their glycan chains, and variants with Manp(alpha1-6) (GIPC B), Manp(alpha1-6) Manp(alpha1-6) (GIPC C), and Manp(alpha1-2)Manp(alpha1-6)Manp(alpha1-6) (GIPC D) substituents linked to the nonreducing terminal mannose residue found in the WT GIPC A were abundant. The ceramide moieties of C. neoformans GIPCs were composed of a C(18) phytosphingosine long-chain base mainly N-acylated with 2-hydroxy-tetracosanoic acid in the WT GIPC while in the acapsular Cap67 mutant GIPCs, as well as 2-hydroxy-tetracosanoic acid, the unusual 2,3-dihydroxy-tetracosanoic acid was characterized. In addition, structural analysis revealed that the amount of GIPC in the WT cells was fourfold less of that in the acapsular mutant."}