PubMed:12039536 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/12039536","sourcedb":"PubMed","sourceid":"12039536","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/12039536","text":"Efficient chemoenzymatic synthesis of globotriose and its derivatives with a recombinant alpha-(1--\u003e4)-galactosyltransferase.\nA truncated alpha-(1--\u003e4)-galactosyltransferase (LgtC) gene from Neisseria meningitidis was cloned. The recombinant glycosyltransferase was expressed in Escherichia coli BL21 (DE3) strain with high specific activity (5 units/mg protein). Its acceptor specificity was carefully characterized. Then the purified enzyme was utilized in highly efficient syntheses of globotriose and a variety of alpha-(1--\u003e4)-galactosylated derivatives as potential antibacterial agents.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":125},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":126,"end":225},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":226,"end":363},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":364,"end":417},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":418,"end":593},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":125},"obj":"Sentence"},{"id":"T2","span":{"begin":126,"end":225},"obj":"Sentence"},{"id":"T3","span":{"begin":226,"end":363},"obj":"Sentence"},{"id":"T4","span":{"begin":364,"end":417},"obj":"Sentence"},{"id":"T5","span":{"begin":418,"end":593},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":191,"end":213},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":279,"end":295},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"487"},{"id":"A2","pred":"db_id","subj":"T2","obj":"562"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#d6ec93","default":true},{"id":"NCBITAXON","color":"#e893ec"}]}]}}