PubMed:11939564 JSONTXT

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cloning of rice semidwarfing gene, sd-1: rice \"green revolution gene\" encodes a mutant enzyme involved in gibberellin synthesis.\nA rice semidwarfing gene, sd-1, known as the \"green revolution gene,\" was isolated by positional cloning and revealed to encode gibberellin 20-oxidase, the key enzyme in the gibberellin biosynthesis pathway. Analysis of 3477 segregants using several PCR-based marker technologies, including cleaved amplified polymorphic sequence, derived-CAPS, and single nucleotide polymorphisms revealed 1 ORF in a 6-kb candidate interval. Normal-type rice cultivars have an identical sequence in this region, consisting of 3 exons (558, 318, and 291 bp) and 2 introns (105 and 1471 bp). Dee-Geo-Woo-Gen-type sd-1 mutants have a 383-bp deletion from the genome (278-bp deletion from the expressed sequence), from the middle of exon 1 to upstream of exon 2, including a 105-bp intron, resulting in a frame-shift that produces a termination codon after the deletion site. The radiation-induced sd-1 mutant Calrose 76 has a 1-bp substitution in exon 2, causing an amino acid substitution (Leu [CTC] to Phe [TTC]). Expression analysis suggests the existence of at least one more locus of gibberellin 20-oxidase which may prevent severe dwarfism from developing in sd-1 mutants."}

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cloning of rice semidwarfing gene, sd-1: rice \"green revolution gene\" encodes a mutant enzyme involved in gibberellin synthesis.\nA rice semidwarfing gene, sd-1, known as the \"green revolution gene,\" was isolated by positional cloning and revealed to encode gibberellin 20-oxidase, the key enzyme in the gibberellin biosynthesis pathway. Analysis of 3477 segregants using several PCR-based marker technologies, including cleaved amplified polymorphic sequence, derived-CAPS, and single nucleotide polymorphisms revealed 1 ORF in a 6-kb candidate interval. Normal-type rice cultivars have an identical sequence in this region, consisting of 3 exons (558, 318, and 291 bp) and 2 introns (105 and 1471 bp). Dee-Geo-Woo-Gen-type sd-1 mutants have a 383-bp deletion from the genome (278-bp deletion from the expressed sequence), from the middle of exon 1 to upstream of exon 2, including a 105-bp intron, resulting in a frame-shift that produces a termination codon after the deletion site. The radiation-induced sd-1 mutant Calrose 76 has a 1-bp substitution in exon 2, causing an amino acid substitution (Leu [CTC] to Phe [TTC]). Expression analysis suggests the existence of at least one more locus of gibberellin 20-oxidase which may prevent severe dwarfism from developing in sd-1 mutants."}

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cloning of rice semidwarfing gene, sd-1: rice \"green revolution gene\" encodes a mutant enzyme involved in gibberellin synthesis.\nA rice semidwarfing gene, sd-1, known as the \"green revolution gene,\" was isolated by positional cloning and revealed to encode gibberellin 20-oxidase, the key enzyme in the gibberellin biosynthesis pathway. Analysis of 3477 segregants using several PCR-based marker technologies, including cleaved amplified polymorphic sequence, derived-CAPS, and single nucleotide polymorphisms revealed 1 ORF in a 6-kb candidate interval. Normal-type rice cultivars have an identical sequence in this region, consisting of 3 exons (558, 318, and 291 bp) and 2 introns (105 and 1471 bp). Dee-Geo-Woo-Gen-type sd-1 mutants have a 383-bp deletion from the genome (278-bp deletion from the expressed sequence), from the middle of exon 1 to upstream of exon 2, including a 105-bp intron, resulting in a frame-shift that produces a termination codon after the deletion site. The radiation-induced sd-1 mutant Calrose 76 has a 1-bp substitution in exon 2, causing an amino acid substitution (Leu [CTC] to Phe [TTC]). Expression analysis suggests the existence of at least one more locus of gibberellin 20-oxidase which may prevent severe dwarfism from developing in sd-1 mutants."}

{"project":"OryzaGP_2021_v2","denotations":[{"id":"T1","span":{"begin":46,"end":50},"obj":"http://identifiers.org/oryzabase.gene/470"},{"id":"T2","span":{"begin":166,"end":170},"obj":"http://identifiers.org/oryzabase.gene/470"},{"id":"T3","span":{"begin":735,"end":739},"obj":"http://identifiers.org/oryzabase.gene/470"},{"id":"T4","span":{"begin":1018,"end":1022},"obj":"http://identifiers.org/oryzabase.gene/470"},{"id":"T5","span":{"begin":1286,"end":1290},"obj":"http://identifiers.org/oryzabase.gene/470"},{"id":"T74334","span":{"begin":46,"end":50},"obj":"http://identifiers.org/rapdb.locus/Os01g0883800"},{"id":"T42675","span":{"begin":166,"end":170},"obj":"http://identifiers.org/rapdb.locus/Os01g0883800"},{"id":"T1431","span":{"begin":735,"end":739},"obj":"http://identifiers.org/rapdb.locus/Os01g0883800"},{"id":"T89881","span":{"begin":1018,"end":1022},"obj":"http://identifiers.org/rapdb.locus/Os01g0883800"},{"id":"T54146","span":{"begin":1286,"end":1290},"obj":"http://identifiers.org/rapdb.locus/Os01g0883800"}],"text":"Positional cloning of rice semidwarfing gene, sd-1: rice \"green revolution gene\" encodes a mutant enzyme involved in gibberellin synthesis.\nA rice semidwarfing gene, sd-1, known as the \"green revolution gene,\" was isolated by positional cloning and revealed to encode gibberellin 20-oxidase, the key enzyme in the gibberellin biosynthesis pathway. Analysis of 3477 segregants using several PCR-based marker technologies, including cleaved amplified polymorphic sequence, derived-CAPS, and single nucleotide polymorphisms revealed 1 ORF in a 6-kb candidate interval. Normal-type rice cultivars have an identical sequence in this region, consisting of 3 exons (558, 318, and 291 bp) and 2 introns (105 and 1471 bp). Dee-Geo-Woo-Gen-type sd-1 mutants have a 383-bp deletion from the genome (278-bp deletion from the expressed sequence), from the middle of exon 1 to upstream of exon 2, including a 105-bp intron, resulting in a frame-shift that produces a termination codon after the deletion site. The radiation-induced sd-1 mutant Calrose 76 has a 1-bp substitution in exon 2, causing an amino acid substitution (Leu [CTC] to Phe [TTC]). Expression analysis suggests the existence of at least one more locus of gibberellin 20-oxidase which may prevent severe dwarfism from developing in sd-1 mutants."}

{"project":"OryzaGP_2022","denotations":[{"id":"T1","span":{"begin":49,"end":50},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T2","span":{"begin":169,"end":170},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T3","span":{"begin":530,"end":531},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T4","span":{"begin":738,"end":739},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T5","span":{"begin":858,"end":859},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T6","span":{"begin":1021,"end":1022},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T7","span":{"begin":1047,"end":1048},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T8","span":{"begin":1289,"end":1290},"obj":"http://identifiers.org/oryzabase.gene/11216"}],"text":"Positional cloning of rice semidwarfing gene, sd-1: rice \"green revolution gene\" encodes a mutant enzyme involved in gibberellin synthesis.\nA rice semidwarfing gene, sd-1, known as the \"green revolution gene,\" was isolated by positional cloning and revealed to encode gibberellin 20-oxidase, the key enzyme in the gibberellin biosynthesis pathway. Analysis of 3477 segregants using several PCR-based marker technologies, including cleaved amplified polymorphic sequence, derived-CAPS, and single nucleotide polymorphisms revealed 1 ORF in a 6-kb candidate interval. Normal-type rice cultivars have an identical sequence in this region, consisting of 3 exons (558, 318, and 291 bp) and 2 introns (105 and 1471 bp). Dee-Geo-Woo-Gen-type sd-1 mutants have a 383-bp deletion from the genome (278-bp deletion from the expressed sequence), from the middle of exon 1 to upstream of exon 2, including a 105-bp intron, resulting in a frame-shift that produces a termination codon after the deletion site. The radiation-induced sd-1 mutant Calrose 76 has a 1-bp substitution in exon 2, causing an amino acid substitution (Leu [CTC] to Phe [TTC]). Expression analysis suggests the existence of at least one more locus of gibberellin 20-oxidase which may prevent severe dwarfism from developing in sd-1 mutants."}

{"project":"OryzaGP_2021_FLAIR","denotations":[{"id":"M_0","span":{"begin":714,"end":747},"obj":"hunflair:NA:Gene"},{"id":"M_1","span":{"begin":1258,"end":1266},"obj":"hunflair:NA:Disease"},{"id":"M_2","span":{"begin":1112,"end":1115},"obj":"hunflair:NA:Chemical"},{"id":"M_3","span":{"begin":46,"end":50},"obj":"hunflair:NA:Gene"},{"id":"M_4","span":{"begin":166,"end":170},"obj":"hunflair:NA:Gene"},{"id":"M_5","span":{"begin":735,"end":739},"obj":"hunflair:NA:Gene"},{"id":"M_6","span":{"begin":1018,"end":1022},"obj":"hunflair:NA:Gene"},{"id":"M_7","span":{"begin":1286,"end":1290},"obj":"hunflair:NA:Gene"},{"id":"M_8","span":{"begin":1087,"end":1097},"obj":"hunflair:NA:Chemical"},{"id":"M_9","span":{"begin":22,"end":26},"obj":"hunflair:NA:Species"},{"id":"M_10","span":{"begin":52,"end":56},"obj":"hunflair:NA:Species"},{"id":"M_11","span":{"begin":142,"end":146},"obj":"hunflair:NA:Species"},{"id":"M_12","span":{"begin":578,"end":582},"obj":"hunflair:NA:Species"},{"id":"M_13","span":{"begin":117,"end":128},"obj":"hunflair:NA:Chemical"},{"id":"M_14","span":{"begin":268,"end":279},"obj":"hunflair:NA:Chemical"},{"id":"M_15","span":{"begin":314,"end":325},"obj":"hunflair:NA:Chemical"},{"id":"M_16","span":{"begin":1210,"end":1221},"obj":"hunflair:NA:Chemical"},{"id":"M_17","span":{"begin":268,"end":290},"obj":"hunflair:NA:Gene"},{"id":"M_18","span":{"begin":1210,"end":1232},"obj":"hunflair:NA:Gene"},{"id":"M_19","span":{"begin":1125,"end":1128},"obj":"hunflair:NA:Chemical"},{"id":"M_20","span":{"begin":117,"end":128},"obj":"hunflair:NA:Gene"},{"id":"M_21","span":{"begin":268,"end":279},"obj":"hunflair:NA:Gene"},{"id":"M_22","span":{"begin":314,"end":325},"obj":"hunflair:NA:Gene"},{"id":"M_23","span":{"begin":1210,"end":1221},"obj":"hunflair:NA:Gene"}],"text":"Positional cloning of rice semidwarfing gene, sd-1: rice \"green revolution gene\" encodes a mutant enzyme involved in gibberellin synthesis.\nA rice semidwarfing gene, sd-1, known as the \"green revolution gene,\" was isolated by positional cloning and revealed to encode gibberellin 20-oxidase, the key enzyme in the gibberellin biosynthesis pathway. Analysis of 3477 segregants using several PCR-based marker technologies, including cleaved amplified polymorphic sequence, derived-CAPS, and single nucleotide polymorphisms revealed 1 ORF in a 6-kb candidate interval. Normal-type rice cultivars have an identical sequence in this region, consisting of 3 exons (558, 318, and 291 bp) and 2 introns (105 and 1471 bp). Dee-Geo-Woo-Gen-type sd-1 mutants have a 383-bp deletion from the genome (278-bp deletion from the expressed sequence), from the middle of exon 1 to upstream of exon 2, including a 105-bp intron, resulting in a frame-shift that produces a termination codon after the deletion site. The radiation-induced sd-1 mutant Calrose 76 has a 1-bp substitution in exon 2, causing an amino acid substitution (Leu [CTC] to Phe [TTC]). Expression analysis suggests the existence of at least one more locus of gibberellin 20-oxidase which may prevent severe dwarfism from developing in sd-1 mutants."}

{"project":"funRiceGenes-all","denotations":[{"id":"PTO-all_T1","span":{"begin":46,"end":48},"obj":"http://purl.obolibrary.org/obo/TO_0000280"},{"id":"PTO-all_T2","span":{"begin":166,"end":168},"obj":"http://purl.obolibrary.org/obo/TO_0000280"},{"id":"PTO-all_T3","span":{"begin":314,"end":338},"obj":"http://purl.obolibrary.org/obo/TO_0000992"},{"id":"PTO-all_T4","span":{"begin":735,"end":737},"obj":"http://purl.obolibrary.org/obo/TO_0000280"},{"id":"PTO-all_T5","span":{"begin":1018,"end":1020},"obj":"http://purl.obolibrary.org/obo/TO_0000280"},{"id":"PTO-all_T6","span":{"begin":1286,"end":1288},"obj":"http://purl.obolibrary.org/obo/TO_0000280"}],"text":"Positional cloning of rice semidwarfing gene, sd-1: rice \"green revolution gene\" encodes a mutant enzyme involved in gibberellin synthesis.\nA rice semidwarfing gene, sd-1, known as the \"green revolution gene,\" was isolated by positional cloning and revealed to encode gibberellin 20-oxidase, the key enzyme in the gibberellin biosynthesis pathway. Analysis of 3477 segregants using several PCR-based marker technologies, including cleaved amplified polymorphic sequence, derived-CAPS, and single nucleotide polymorphisms revealed 1 ORF in a 6-kb candidate interval. Normal-type rice cultivars have an identical sequence in this region, consisting of 3 exons (558, 318, and 291 bp) and 2 introns (105 and 1471 bp). Dee-Geo-Woo-Gen-type sd-1 mutants have a 383-bp deletion from the genome (278-bp deletion from the expressed sequence), from the middle of exon 1 to upstream of exon 2, including a 105-bp intron, resulting in a frame-shift that produces a termination codon after the deletion site. The radiation-induced sd-1 mutant Calrose 76 has a 1-bp substitution in exon 2, causing an amino acid substitution (Leu [CTC] to Phe [TTC]). Expression analysis suggests the existence of at least one more locus of gibberellin 20-oxidase which may prevent severe dwarfism from developing in sd-1 mutants."}