PubMed:11815617 JSONTXT

{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":119},"obj":"Sentence"},{"id":"T2","span":{"begin":120,"end":275},"obj":"Sentence"},{"id":"T3","span":{"begin":276,"end":452},"obj":"Sentence"},{"id":"T4","span":{"begin":453,"end":587},"obj":"Sentence"},{"id":"T5","span":{"begin":588,"end":758},"obj":"Sentence"},{"id":"T6","span":{"begin":759,"end":816},"obj":"Sentence"},{"id":"T7","span":{"begin":817,"end":961},"obj":"Sentence"},{"id":"T8","span":{"begin":962,"end":1061},"obj":"Sentence"},{"id":"T9","span":{"begin":1062,"end":1191},"obj":"Sentence"},{"id":"T10","span":{"begin":1192,"end":1349},"obj":"Sentence"},{"id":"T11","span":{"begin":1350,"end":1519},"obj":"Sentence"},{"id":"T12","span":{"begin":1520,"end":1655},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Two proteins essential for apolipoprotein B mRNA editing are expressed from a single gene through alternative splicing.\nApolipoprotein B (apoB) mRNA editing involves site-specific deamination of cytidine to form uridine, resulting in the production of an in-frame stop codon. Protein translated from edited mRNA is associated with a reduced risk of atherosclerosis, and hence the protein factors that regulate hepatic apoB mRNA editing are of interest. A human protein essential for apoB mRNA editing and an eight-amino acid-longer variant of no known function have been recently cloned. We report that both proteins, henceforth referred to as ACF64 and ACF65, supported APOBEC-1 (the catalytic subunit of the editosome) equivalently in editing of apoB mRNA. They are encoded by a single 82-kb gene on chromosome 10. The transcripts are encoded by 15 exons that are expressed from a tissue-specific promoter minimally contained within the -0.33-kb DNA sequence. ACF64 and ACF65 mRNAs are expressed in both liver and intestinal cells in an approximate 1:4 ratio. Exon 11 is alternatively spliced to include or exclude 24 nucleotides of exon 12, thereby encoding ACF65 and ACF64, respectively. Recognition motifs for the serine/arginine-rich (SR) proteins SC35, SRp40, SRp55, and SF2/ASF involved in alternative RNA splicing were predicted in exon 12. Overexpression of these SR proteins in liver cells demonstrated that alternative splicing of a minigene-derived transcript to express ACF65 was enhanced 6-fold by SRp40. The data account for the expression of two editing factors and provide a possible explanation for their different levels of expression."}

{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":1006,"end":1011},"obj":"http://purl.obolibrary.org/obo/MAT_0000097"},{"id":"T2","span":{"begin":1016,"end":1026},"obj":"http://purl.obolibrary.org/obo/MAT_0000043"},{"id":"T3","span":{"begin":1389,"end":1394},"obj":"http://purl.obolibrary.org/obo/MAT_0000097"}],"text":"Two proteins essential for apolipoprotein B mRNA editing are expressed from a single gene through alternative splicing.\nApolipoprotein B (apoB) mRNA editing involves site-specific deamination of cytidine to form uridine, resulting in the production of an in-frame stop codon. Protein translated from edited mRNA is associated with a reduced risk of atherosclerosis, and hence the protein factors that regulate hepatic apoB mRNA editing are of interest. A human protein essential for apoB mRNA editing and an eight-amino acid-longer variant of no known function have been recently cloned. We report that both proteins, henceforth referred to as ACF64 and ACF65, supported APOBEC-1 (the catalytic subunit of the editosome) equivalently in editing of apoB mRNA. They are encoded by a single 82-kb gene on chromosome 10. The transcripts are encoded by 15 exons that are expressed from a tissue-specific promoter minimally contained within the -0.33-kb DNA sequence. ACF64 and ACF65 mRNAs are expressed in both liver and intestinal cells in an approximate 1:4 ratio. Exon 11 is alternatively spliced to include or exclude 24 nucleotides of exon 12, thereby encoding ACF65 and ACF64, respectively. Recognition motifs for the serine/arginine-rich (SR) proteins SC35, SRp40, SRp55, and SF2/ASF involved in alternative RNA splicing were predicted in exon 12. Overexpression of these SR proteins in liver cells demonstrated that alternative splicing of a minigene-derived transcript to express ACF65 was enhanced 6-fold by SRp40. The data account for the expression of two editing factors and provide a possible explanation for their different levels of expression."}

{"project":"FSU-PRGE","denotations":[{"id":"T1","span":{"begin":27,"end":43},"obj":"protein"},{"id":"T2","span":{"begin":120,"end":136},"obj":"protein"},{"id":"T3","span":{"begin":138,"end":142},"obj":"protein"},{"id":"T4","span":{"begin":418,"end":422},"obj":"protein"},{"id":"T5","span":{"begin":483,"end":487},"obj":"protein"},{"id":"T6","span":{"begin":644,"end":649},"obj":"protein"},{"id":"T7","span":{"begin":654,"end":659},"obj":"protein"},{"id":"T8","span":{"begin":671,"end":679},"obj":"protein"},{"id":"T9","span":{"begin":748,"end":752},"obj":"protein"},{"id":"T10","span":{"begin":962,"end":967},"obj":"protein"},{"id":"T11","span":{"begin":972,"end":977},"obj":"protein"},{"id":"T12","span":{"begin":1161,"end":1166},"obj":"protein"},{"id":"T13","span":{"begin":1171,"end":1176},"obj":"protein"},{"id":"T14","span":{"begin":1254,"end":1258},"obj":"protein"},{"id":"T15","span":{"begin":1260,"end":1265},"obj":"protein"},{"id":"T16","span":{"begin":1267,"end":1272},"obj":"protein"},{"id":"T17","span":{"begin":1278,"end":1281},"obj":"protein"},{"id":"T18","span":{"begin":1282,"end":1285},"obj":"protein"},{"id":"T19","span":{"begin":1484,"end":1489},"obj":"protein"},{"id":"T20","span":{"begin":1513,"end":1518},"obj":"protein"}],"text":"Two proteins essential for apolipoprotein B mRNA editing are expressed from a single gene through alternative splicing.\nApolipoprotein B (apoB) mRNA editing involves site-specific deamination of cytidine to form uridine, resulting in the production of an in-frame stop codon. Protein translated from edited mRNA is associated with a reduced risk of atherosclerosis, and hence the protein factors that regulate hepatic apoB mRNA editing are of interest. A human protein essential for apoB mRNA editing and an eight-amino acid-longer variant of no known function have been recently cloned. We report that both proteins, henceforth referred to as ACF64 and ACF65, supported APOBEC-1 (the catalytic subunit of the editosome) equivalently in editing of apoB mRNA. They are encoded by a single 82-kb gene on chromosome 10. The transcripts are encoded by 15 exons that are expressed from a tissue-specific promoter minimally contained within the -0.33-kb DNA sequence. ACF64 and ACF65 mRNAs are expressed in both liver and intestinal cells in an approximate 1:4 ratio. Exon 11 is alternatively spliced to include or exclude 24 nucleotides of exon 12, thereby encoding ACF65 and ACF64, respectively. Recognition motifs for the serine/arginine-rich (SR) proteins SC35, SRp40, SRp55, and SF2/ASF involved in alternative RNA splicing were predicted in exon 12. Overexpression of these SR proteins in liver cells demonstrated that alternative splicing of a minigene-derived transcript to express ACF65 was enhanced 6-fold by SRp40. The data account for the expression of two editing factors and provide a possible explanation for their different levels of expression."}

{"project":"PIR-corpus2","denotations":[{"id":"T1","span":{"begin":27,"end":43},"obj":"protein"},{"id":"T2","span":{"begin":121,"end":143},"obj":"protein"},{"id":"T3","span":{"begin":410,"end":422},"obj":"protein"},{"id":"T4","span":{"begin":483,"end":487},"obj":"protein"},{"id":"T5","span":{"begin":644,"end":649},"obj":"protein"},{"id":"T6","span":{"begin":654,"end":659},"obj":"protein"},{"id":"T7","span":{"begin":671,"end":679},"obj":"protein"},{"id":"T8","span":{"begin":685,"end":719},"obj":"protein"},{"id":"T9","span":{"begin":748,"end":752},"obj":"protein"},{"id":"T10","span":{"begin":962,"end":967},"obj":"protein"},{"id":"T11","span":{"begin":972,"end":977},"obj":"protein"},{"id":"T12","span":{"begin":1161,"end":1166},"obj":"protein"},{"id":"T13","span":{"begin":1171,"end":1176},"obj":"protein"},{"id":"T14","span":{"begin":1219,"end":1258},"obj":"protein"},{"id":"T15","span":{"begin":1260,"end":1265},"obj":"protein"},{"id":"T16","span":{"begin":1267,"end":1272},"obj":"protein"},{"id":"T17","span":{"begin":1278,"end":1281},"obj":"protein"},{"id":"T18","span":{"begin":1282,"end":1285},"obj":"protein"},{"id":"T19","span":{"begin":1374,"end":1385},"obj":"protein"},{"id":"T20","span":{"begin":1484,"end":1489},"obj":"protein"},{"id":"T21","span":{"begin":1513,"end":1518},"obj":"protein"}],"text":"Two proteins essential for apolipoprotein B mRNA editing are expressed from a single gene through alternative splicing.\nApolipoprotein B (apoB) mRNA editing involves site-specific deamination of cytidine to form uridine, resulting in the production of an in-frame stop codon. Protein translated from edited mRNA is associated with a reduced risk of atherosclerosis, and hence the protein factors that regulate hepatic apoB mRNA editing are of interest. A human protein essential for apoB mRNA editing and an eight-amino acid-longer variant of no known function have been recently cloned. We report that both proteins, henceforth referred to as ACF64 and ACF65, supported APOBEC-1 (the catalytic subunit of the editosome) equivalently in editing of apoB mRNA. They are encoded by a single 82-kb gene on chromosome 10. The transcripts are encoded by 15 exons that are expressed from a tissue-specific promoter minimally contained within the -0.33-kb DNA sequence. ACF64 and ACF65 mRNAs are expressed in both liver and intestinal cells in an approximate 1:4 ratio. Exon 11 is alternatively spliced to include or exclude 24 nucleotides of exon 12, thereby encoding ACF65 and ACF64, respectively. Recognition motifs for the serine/arginine-rich (SR) proteins SC35, SRp40, SRp55, and SF2/ASF involved in alternative RNA splicing were predicted in exon 12. Overexpression of these SR proteins in liver cells demonstrated that alternative splicing of a minigene-derived transcript to express ACF65 was enhanced 6-fold by SRp40. The data account for the expression of two editing factors and provide a possible explanation for their different levels of expression."}

{"project":"PIR-corpus1","denotations":[{"id":"T1","span":{"begin":4,"end":12},"obj":"protein"},{"id":"T2","span":{"begin":121,"end":136},"obj":"protein"},{"id":"T3","span":{"begin":138,"end":142},"obj":"acronym"},{"id":"T4","span":{"begin":276,"end":283},"obj":"protein"},{"id":"T5","span":{"begin":380,"end":395},"obj":"protein"},{"id":"T6","span":{"begin":461,"end":468},"obj":"protein"},{"id":"T7","span":{"begin":608,"end":616},"obj":"protein"},{"id":"T8","span":{"begin":644,"end":649},"obj":"protein"},{"id":"T9","span":{"begin":654,"end":659},"obj":"protein"},{"id":"T10","span":{"begin":671,"end":679},"obj":"protein"},{"id":"T11","span":{"begin":685,"end":719},"obj":"protein"},{"id":"T12","span":{"begin":1161,"end":1166},"obj":"protein"},{"id":"T13","span":{"begin":1171,"end":1176},"obj":"protein"},{"id":"T14","span":{"begin":1219,"end":1253},"obj":"protein"},{"id":"T15","span":{"begin":1254,"end":1258},"obj":"protein"},{"id":"T16","span":{"begin":1260,"end":1265},"obj":"protein"},{"id":"T17","span":{"begin":1267,"end":1272},"obj":"protein"},{"id":"T18","span":{"begin":1278,"end":1285},"obj":"protein"},{"id":"T19","span":{"begin":1374,"end":1385},"obj":"protein"},{"id":"T20","span":{"begin":1484,"end":1489},"obj":"protein"},{"id":"T21","span":{"begin":1513,"end":1518},"obj":"protein"},{"id":"T22","span":{"begin":1563,"end":1578},"obj":"protein"}],"text":"Two proteins essential for apolipoprotein B mRNA editing are expressed from a single gene through alternative splicing.\nApolipoprotein B (apoB) mRNA editing involves site-specific deamination of cytidine to form uridine, resulting in the production of an in-frame stop codon. Protein translated from edited mRNA is associated with a reduced risk of atherosclerosis, and hence the protein factors that regulate hepatic apoB mRNA editing are of interest. A human protein essential for apoB mRNA editing and an eight-amino acid-longer variant of no known function have been recently cloned. We report that both proteins, henceforth referred to as ACF64 and ACF65, supported APOBEC-1 (the catalytic subunit of the editosome) equivalently in editing of apoB mRNA. They are encoded by a single 82-kb gene on chromosome 10. The transcripts are encoded by 15 exons that are expressed from a tissue-specific promoter minimally contained within the -0.33-kb DNA sequence. ACF64 and ACF65 mRNAs are expressed in both liver and intestinal cells in an approximate 1:4 ratio. Exon 11 is alternatively spliced to include or exclude 24 nucleotides of exon 12, thereby encoding ACF65 and ACF64, respectively. Recognition motifs for the serine/arginine-rich (SR) proteins SC35, SRp40, SRp55, and SF2/ASF involved in alternative RNA splicing were predicted in exon 12. Overexpression of these SR proteins in liver cells demonstrated that alternative splicing of a minigene-derived transcript to express ACF65 was enhanced 6-fold by SRp40. The data account for the expression of two editing factors and provide a possible explanation for their different levels of expression."}

{"project":"HP-phenotype","denotations":[{"id":"T1","span":{"begin":349,"end":364},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0002621"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}],"text":"Two proteins essential for apolipoprotein B mRNA editing are expressed from a single gene through alternative splicing.\nApolipoprotein B (apoB) mRNA editing involves site-specific deamination of cytidine to form uridine, resulting in the production of an in-frame stop codon. Protein translated from edited mRNA is associated with a reduced risk of atherosclerosis, and hence the protein factors that regulate hepatic apoB mRNA editing are of interest. A human protein essential for apoB mRNA editing and an eight-amino acid-longer variant of no known function have been recently cloned. We report that both proteins, henceforth referred to as ACF64 and ACF65, supported APOBEC-1 (the catalytic subunit of the editosome) equivalently in editing of apoB mRNA. They are encoded by a single 82-kb gene on chromosome 10. The transcripts are encoded by 15 exons that are expressed from a tissue-specific promoter minimally contained within the -0.33-kb DNA sequence. ACF64 and ACF65 mRNAs are expressed in both liver and intestinal cells in an approximate 1:4 ratio. Exon 11 is alternatively spliced to include or exclude 24 nucleotides of exon 12, thereby encoding ACF65 and ACF64, respectively. Recognition motifs for the serine/arginine-rich (SR) proteins SC35, SRp40, SRp55, and SF2/ASF involved in alternative RNA splicing were predicted in exon 12. Overexpression of these SR proteins in liver cells demonstrated that alternative splicing of a minigene-derived transcript to express ACF65 was enhanced 6-fold by SRp40. The data account for the expression of two editing factors and provide a possible explanation for their different levels of expression."}

{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":349,"end":364},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0005311"}],"text":"Two proteins essential for apolipoprotein B mRNA editing are expressed from a single gene through alternative splicing.\nApolipoprotein B (apoB) mRNA editing involves site-specific deamination of cytidine to form uridine, resulting in the production of an in-frame stop codon. Protein translated from edited mRNA is associated with a reduced risk of atherosclerosis, and hence the protein factors that regulate hepatic apoB mRNA editing are of interest. A human protein essential for apoB mRNA editing and an eight-amino acid-longer variant of no known function have been recently cloned. We report that both proteins, henceforth referred to as ACF64 and ACF65, supported APOBEC-1 (the catalytic subunit of the editosome) equivalently in editing of apoB mRNA. They are encoded by a single 82-kb gene on chromosome 10. The transcripts are encoded by 15 exons that are expressed from a tissue-specific promoter minimally contained within the -0.33-kb DNA sequence. ACF64 and ACF65 mRNAs are expressed in both liver and intestinal cells in an approximate 1:4 ratio. Exon 11 is alternatively spliced to include or exclude 24 nucleotides of exon 12, thereby encoding ACF65 and ACF64, respectively. Recognition motifs for the serine/arginine-rich (SR) proteins SC35, SRp40, SRp55, and SF2/ASF involved in alternative RNA splicing were predicted in exon 12. Overexpression of these SR proteins in liver cells demonstrated that alternative splicing of a minigene-derived transcript to express ACF65 was enhanced 6-fold by SRp40. The data account for the expression of two editing factors and provide a possible explanation for their different levels of expression."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":455,"end":460},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"}],"text":"Two proteins essential for apolipoprotein B mRNA editing are expressed from a single gene through alternative splicing.\nApolipoprotein B (apoB) mRNA editing involves site-specific deamination of cytidine to form uridine, resulting in the production of an in-frame stop codon. Protein translated from edited mRNA is associated with a reduced risk of atherosclerosis, and hence the protein factors that regulate hepatic apoB mRNA editing are of interest. A human protein essential for apoB mRNA editing and an eight-amino acid-longer variant of no known function have been recently cloned. We report that both proteins, henceforth referred to as ACF64 and ACF65, supported APOBEC-1 (the catalytic subunit of the editosome) equivalently in editing of apoB mRNA. They are encoded by a single 82-kb gene on chromosome 10. The transcripts are encoded by 15 exons that are expressed from a tissue-specific promoter minimally contained within the -0.33-kb DNA sequence. ACF64 and ACF65 mRNAs are expressed in both liver and intestinal cells in an approximate 1:4 ratio. Exon 11 is alternatively spliced to include or exclude 24 nucleotides of exon 12, thereby encoding ACF65 and ACF64, respectively. Recognition motifs for the serine/arginine-rich (SR) proteins SC35, SRp40, SRp55, and SF2/ASF involved in alternative RNA splicing were predicted in exon 12. Overexpression of these SR proteins in liver cells demonstrated that alternative splicing of a minigene-derived transcript to express ACF65 was enhanced 6-fold by SRp40. The data account for the expression of two editing factors and provide a possible explanation for their different levels of expression."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":802,"end":812},"obj":"Body_part"},{"id":"T2","span":{"begin":883,"end":889},"obj":"Body_part"},{"id":"T3","span":{"begin":1006,"end":1011},"obj":"Body_part"},{"id":"T4","span":{"begin":1389,"end":1394},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/GO_0005694"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0002107"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0002107"}],"text":"Two proteins essential for apolipoprotein B mRNA editing are expressed from a single gene through alternative splicing.\nApolipoprotein B (apoB) mRNA editing involves site-specific deamination of cytidine to form uridine, resulting in the production of an in-frame stop codon. Protein translated from edited mRNA is associated with a reduced risk of atherosclerosis, and hence the protein factors that regulate hepatic apoB mRNA editing are of interest. A human protein essential for apoB mRNA editing and an eight-amino acid-longer variant of no known function have been recently cloned. We report that both proteins, henceforth referred to as ACF64 and ACF65, supported APOBEC-1 (the catalytic subunit of the editosome) equivalently in editing of apoB mRNA. They are encoded by a single 82-kb gene on chromosome 10. The transcripts are encoded by 15 exons that are expressed from a tissue-specific promoter minimally contained within the -0.33-kb DNA sequence. ACF64 and ACF65 mRNAs are expressed in both liver and intestinal cells in an approximate 1:4 ratio. Exon 11 is alternatively spliced to include or exclude 24 nucleotides of exon 12, thereby encoding ACF65 and ACF64, respectively. Recognition motifs for the serine/arginine-rich (SR) proteins SC35, SRp40, SRp55, and SF2/ASF involved in alternative RNA splicing were predicted in exon 12. Overexpression of these SR proteins in liver cells demonstrated that alternative splicing of a minigene-derived transcript to express ACF65 was enhanced 6-fold by SRp40. The data account for the expression of two editing factors and provide a possible explanation for their different levels of expression."}

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":1006,"end":1011},"obj":"Body_part"},{"id":"T2","span":{"begin":1016,"end":1026},"obj":"Body_part"},{"id":"T3","span":{"begin":1389,"end":1394},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000097"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000043"},{"id":"A3","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000097"}],"text":"Two proteins essential for apolipoprotein B mRNA editing are expressed from a single gene through alternative splicing.\nApolipoprotein B (apoB) mRNA editing involves site-specific deamination of cytidine to form uridine, resulting in the production of an in-frame stop codon. Protein translated from edited mRNA is associated with a reduced risk of atherosclerosis, and hence the protein factors that regulate hepatic apoB mRNA editing are of interest. A human protein essential for apoB mRNA editing and an eight-amino acid-longer variant of no known function have been recently cloned. We report that both proteins, henceforth referred to as ACF64 and ACF65, supported APOBEC-1 (the catalytic subunit of the editosome) equivalently in editing of apoB mRNA. They are encoded by a single 82-kb gene on chromosome 10. The transcripts are encoded by 15 exons that are expressed from a tissue-specific promoter minimally contained within the -0.33-kb DNA sequence. ACF64 and ACF65 mRNAs are expressed in both liver and intestinal cells in an approximate 1:4 ratio. Exon 11 is alternatively spliced to include or exclude 24 nucleotides of exon 12, thereby encoding ACF65 and ACF64, respectively. Recognition motifs for the serine/arginine-rich (SR) proteins SC35, SRp40, SRp55, and SF2/ASF involved in alternative RNA splicing were predicted in exon 12. Overexpression of these SR proteins in liver cells demonstrated that alternative splicing of a minigene-derived transcript to express ACF65 was enhanced 6-fold by SRp40. The data account for the expression of two editing factors and provide a possible explanation for their different levels of expression."}