PubMed:11439191 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/11439191","sourcedb":"PubMed","sourceid":"11439191","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/11439191","text":"Novel sulfated lymphocyte homing receptors and their control by a Core1 extension beta 1,3-N-acetylglucosaminyltransferase.\nL-selectin mediates lymphocyte homing by facilitating lymphocyte adhesion to addressins expressed in the high endothelial venules (HEV) of secondary lymphoid organs. Peripheral node addressin recognized by the MECA-79 antibody is apparently part of the L-selectin ligand, but its chemical nature has been undefined. We now identify a sulfated extended core1 mucin-type O-glycan, Gal beta 1--\u003e4(sulfo--\u003e6)GlcNAc beta 1--\u003e3Gal beta 1--\u003e3GalNAc, as the MECA-79 epitope. Molecular cloning of a HEV-expressed core1-beta 1,3-N-acetylglucosaminyltransferase (Core1-beta 3GlcNAcT) enabled the construction of the 6-sulfo sialyl Lewis x on extended core1 O-glycans, recapitulating the potent L-selectin-mediated, shear-dependent adhesion observed with novel L-selectin ligands derived from core2 beta1,6-N-acetylglucosaminyltransferase-I null mice. These results identify Core1-beta 3GlcNAcT and its cognate extended core1 O-glycans as essential participants in the expression of the MECA-79-positive, HEV-specific L-selectin ligands required for lymphocyte homing.","tracks":[{"project":"GGDB-2020","denotations":[{"id":"T1","span":{"begin":744,"end":751},"obj":"https://acgg.asia/db/ggdb/info/gg004"},{"id":"T2","span":{"begin":744,"end":751},"obj":"https://acgg.asia/db/ggdb/info/gg005"},{"id":"T3","span":{"begin":744,"end":751},"obj":"https://acgg.asia/db/ggdb/info/gg006"},{"id":"T4","span":{"begin":744,"end":751},"obj":"https://acgg.asia/db/ggdb/info/gg007"},{"id":"T5","span":{"begin":744,"end":751},"obj":"https://acgg.asia/db/ggdb/info/gg008"},{"id":"T6","span":{"begin":744,"end":751},"obj":"https://acgg.asia/db/ggdb/info/gg003"},{"id":"T7","span":{"begin":911,"end":952},"obj":"https://acgg.asia/db/ggdb/info/gg111"}],"attributes":[{"subj":"T1","pred":"source","obj":"GGDB-2020"},{"subj":"T2","pred":"source","obj":"GGDB-2020"},{"subj":"T3","pred":"source","obj":"GGDB-2020"},{"subj":"T4","pred":"source","obj":"GGDB-2020"},{"subj":"T5","pred":"source","obj":"GGDB-2020"},{"subj":"T6","pred":"source","obj":"GGDB-2020"},{"subj":"T7","pred":"source","obj":"GGDB-2020"}]},{"project":"ggdb-test","denotations":[{"id":"T1","span":{"begin":744,"end":751},"obj":"https://acgg.asia/db/ggdb/info/gg003"},{"id":"T2","span":{"begin":744,"end":751},"obj":"https://acgg.asia/db/ggdb/info/gg004"},{"id":"T3","span":{"begin":744,"end":751},"obj":"https://acgg.asia/db/ggdb/info/gg005"},{"id":"T4","span":{"begin":744,"end":751},"obj":"https://acgg.asia/db/ggdb/info/gg006"},{"id":"T5","span":{"begin":744,"end":751},"obj":"https://acgg.asia/db/ggdb/info/gg007"},{"id":"T6","span":{"begin":744,"end":751},"obj":"https://acgg.asia/db/ggdb/info/gg008"},{"id":"T7","span":{"begin":911,"end":952},"obj":"https://acgg.asia/db/ggdb/info/gg111"}],"attributes":[{"subj":"T1","pred":"source","obj":"ggdb-test"},{"subj":"T2","pred":"source","obj":"ggdb-test"},{"subj":"T3","pred":"source","obj":"ggdb-test"},{"subj":"T4","pred":"source","obj":"ggdb-test"},{"subj":"T5","pred":"source","obj":"ggdb-test"},{"subj":"T6","pred":"source","obj":"ggdb-test"},{"subj":"T7","pred":"source","obj":"ggdb-test"}]},{"project":"glycogenes","denotations":[{"id":"PD-GlycoGenes20190927-B_T1","span":{"begin":420,"end":423},"obj":"https://acgg.asia/db/ggdb/info/gg135"}],"attributes":[{"subj":"PD-GlycoGenes20190927-B_T1","pred":"source","obj":"glycogenes"}]},{"project":"NGLY1-deficiency","denotations":[{"id":"PD-NGLY1-deficiency-B_T1","span":{"begin":528,"end":534},"obj":"chem:24139"}],"namespaces":[{"prefix":"hgnc","uri":"https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:"},{"prefix":"omim","uri":"https://www.omim.org/entry/"},{"prefix":"chem","uri":"https://pubchem.ncbi.nlm.nih.gov/compound/"}],"attributes":[{"subj":"PD-NGLY1-deficiency-B_T1","pred":"source","obj":"NGLY1-deficiency"}]},{"project":"GlyCosmos15-Glycan","denotations":[{"id":"T1","span":{"begin":744,"end":751},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G00051MO"},{"id":"A2","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00051MO"},{"subj":"T1","pred":"source","obj":"GlyCosmos15-Glycan"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":958,"end":962},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"10088"},{"subj":"T1","pred":"source","obj":"NCBITAXON"}]},{"project":"Glycosmos15-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":729,"end":751},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"}],"attributes":[{"id":"A1","pred":"glycoepitope_id","subj":"T1","obj":"http://www.glycoepitope.jp/epitopes/EP0014"},{"subj":"T1","pred":"source","obj":"Glycosmos15-GlycoEpitope"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":15,"end":25},"obj":"Body_part"},{"id":"T2","span":{"begin":72,"end":81},"obj":"Body_part"},{"id":"T3","span":{"begin":144,"end":154},"obj":"Body_part"},{"id":"T4","span":{"begin":178,"end":188},"obj":"Body_part"},{"id":"T5","span":{"begin":229,"end":253},"obj":"Body_part"},{"id":"T6","span":{"begin":255,"end":258},"obj":"Body_part"},{"id":"T7","span":{"begin":273,"end":288},"obj":"Body_part"},{"id":"T8","span":{"begin":301,"end":305},"obj":"Body_part"},{"id":"T9","span":{"begin":614,"end":617},"obj":"Body_part"},{"id":"T10","span":{"begin":1117,"end":1120},"obj":"Body_part"},{"id":"T11","span":{"begin":1162,"end":1172},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL_0000542"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_2000106"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL_0000542"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL_0000542"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_8410037"},{"id":"A6","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/UBERON_8410037"},{"id":"A7","pred":"uberon_i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