PubMed:11274152 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/11274152","sourcedb":"PubMed","sourceid":"11274152","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/11274152","text":"Disruption of the interaction of mammalian protein synthesis eukaryotic initiation factor 4B with the poly(A)-binding protein by caspase- and viral protease-mediated cleavages.\nEukaryotic initiation factor (eIF) 4B interacts with several components of the initiation pathway and is targeted for cleavage during apoptosis. In a cell-free system, cleavage of eIF4B by caspase-3 coincides with a general inhibition of protein synthetic activity. Affinity chromatography demonstrates that mammalian eIF4B interacts with the poly(A)-binding protein and that a region consisting of the N-terminal 80 amino acids of eIF4B is both necessary and sufficient for such binding. This interaction is lost when eIF4B is cleaved by caspase-3, which removes the N-terminal 45 amino acids. Similarly, the association of eIF4B with the poly(A)-binding protein in vivo is reduced when cells are induced to undergo apoptosis. Cleavage of the poly(A)-binding protein itself, using human rhinovirus 3C protease, also eliminates the interaction with eIF4B. Thus, disruption of the association between mammalian eIF4B and the poly(A)-binding protein can occur during both apoptosis and picornaviral infection and is likely to contribute to the inhibition of translation observed under these conditions.","tracks":[{"project":"2015-BEL-Sample","denotations":[{"id":"T1","span":{"begin":322,"end":441},"obj":"cat(p(HGNC:CASP3)) increases deg(p(HGNC:EIF4B))"}],"attributes":[{"subj":"T1","pred":"source","obj":"2015-BEL-Sample"}]},{"project":"2015-BEL-Sample-2","denotations":[{"id":"BEL:20000284","span":{"begin":772,"end":903},"obj":"bp(GOBP:\"apoptotic process\") decreases complex(p(HGNC:EIF4B),p(HGNC:PABPN1))"},{"id":"BEL:20000286","span":{"begin":322,"end":441},"obj":"cat(p(HGNC:CASP3)) increases deg(p(HGNC:EIF4B))"},{"id":"BEL:20000282","span":{"begin":177,"end":320},"obj":"bp(GOBP:\"apoptotic process\") increases deg(p(HGNC:EIF4B))"},{"id":"BEL:20000284","span":{"begin":772,"end":903},"obj":"bp(GOBP:\"apoptotic process\") decreases 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