| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-172 |
Sentence |
denotes |
Insulin-like growth factor-binding protein-3 modulates expression of Bax and Bcl-2 and potentiates p53-independent radiation-induced apoptosis in human breast cancer cells. |
| T2 |
173-377 |
Sentence |
denotes |
We report that transfection of insulin-like growth factor-binding protein-3 (IGFBP-3) cDNA in human breast cancer cell lines expressing either mutant p53 (T47D) or wild-type p53 (MCF-7) induces apoptosis. |
| T3 |
378-474 |
Sentence |
denotes |
IGFBP-3 also increases the ratio of pro-apoptotic to anti-apoptotic members of the Bcl-2 family. |
| T4 |
475-607 |
Sentence |
denotes |
In MCF-7, an increase in Bad and Bax protein expression and a decrease in Bcl-x(L) protein and Bcl-2 protein and mRNA were observed. |
| T5 |
608-702 |
Sentence |
denotes |
In T47D, Bax and Bad proteins were up-regulated; Bcl-2 protein is undetectable in these cells. |
| T6 |
703-835 |
Sentence |
denotes |
As T47D expresses mutant p53 protein, these modulations of pro-apoptotic proteins and induction of apoptosis are independent of p53. |
| T7 |
836-922 |
Sentence |
denotes |
The effect of IGFBP-3 on the response of T47D to ionizing radiation (IR) was examined. |
| T8 |
923-1006 |
Sentence |
denotes |
These cells do not G(1) arrest in response to IR and are relatively radioresistant. |
| T9 |
1007-1144 |
Sentence |
denotes |
Transfection of IGFBP-3 increased the radiosensitivity of T47D and increased IR-induced apoptosis but did not effect a rapid G(1) arrest. |
| T10 |
1145-1254 |
Sentence |
denotes |
IR also caused a much greater increase in Bax protein in IGFBP-3 transfectants compared with vector controls. |
| T11 |
1255-1504 |
Sentence |
denotes |
Thus, IGFBP-3 increases the expression of pro-apoptotic proteins and apoptosis both basally and in response to IR, suggesting it may be a p53-independent effector of apoptosis in breast cancer cells via its modulation of the Bax:Bcl-2 protein ratio. |
