PubMed:10814825 JSONTXT

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{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10814825","sourcedb":"PubMed","sourceid":"10814825","text":"Naringenin 7-O-methyltransferase involved in the biosynthesis of the flavanone phytoalexin sakuranetin from rice (Oryza sativa L.).\nAn inducible S-adenosyl-L-methionine:naringenin 7-O-methyltransferase (NOMT) catalyzing the methylation of naringenin to sakuranetin, a major rice phytoalexin was purified approximately 985-fold from ultraviolet (UV)-irradiated rice leaves. The enzyme is not found in healthy tissues and was purified to a nearly homogeneous preparation in one step using adenosine-agarose affinity chromatography, with 1 g rice leaves (UV-irradiated) as starting material. Gel filtration chromatography resulted in an almost pure enzyme, as evidenced by a major band migrating to a position corresponding to a molecular mass of approximately 41 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The purified NOMT was strongly inhibited by Mn(2+), Ni(2+), Cu(2+), Zn(2+), Hg(2+), and Cd(2+), and to a low degree by Co(2+), Mg(2+), Ba(2+), Ca(2+) and ethylenediamine tetraacetic acid. The amino acid sequence of a NOMT cyanogen bromide (CNBr)-cleavage peptide was highly homologous to that of a caffeic acid 3-O-methyltransferase from maize, and about 70% of the amino acid sequence was obtained after sequencing the peptides generated by CNBr and/or formic acid hydrolysis. NOMT was also shown to be induced in a time-dependent manner, and purified from rice leaves treated with jasmonic acid and copper chloride.","tracks":[{"project":"Oryza-OGER","denotations":[{"id":"T1","span":{"begin":0,"end":10},"obj":"http://purl.obolibrary.org/obo/CHEBI_50202"},{"id":"T2","span":{"begin":0,"end":10},"obj":"http://id.nlm.nih.gov/mesh/C005273"},{"id":"T3","span":{"begin":0,"end":10},"obj":"http://id.nlm.nih.gov/mesh/C005273"},{"id":"T4","span":{"begin":0,"end":10},"obj":"http://purl.obolibrary.org/obo/CHEBI_17846"},{"id":"T5","span":{"begin":0,"end":32},"obj":"http://id.nlm.nih.gov/mesh/C099725"},{"id":"T6","span":{"begin":0,"end":32},"obj":"NOMT"},{"id":"T7","span":{"begin":0,"end":32},"obj":"http://www.uniprot.org/uniprot/Q0IP69"},{"id":"T8","span":{"begin":49,"end":61},"obj":"http://purl.obolibrary.org/obo/GO_0009058"},{"id":"T9","span":{"begin":69,"end":78},"obj":"http://id.nlm.nih.gov/mesh/C028610"},{"id":"T10","span":{"begin":69,"end":78},"obj":"http://id.nlm.nih.gov/mesh/C028610"},{"id":"T11","span":{"begin":69,"end":78},"obj":"http://purl.obolibrary.org/obo/CHEBI_5070"},{"id":"T12","span":{"begin":69,"end":78},"obj":"http://id.nlm.nih.gov/mesh/C028610"},{"id":"T13","span":{"begin":69,"end":78},"obj":"http://id.nlm.nih.gov/mesh/C028610"},{"id":"T14","span":{"begin":79,"end":90},"obj":"http://id.nlm.nih.gov/mesh/C011991"},{"id":"T15","span":{"begin":79,"end":90},"obj":"http://id.nlm.nih.gov/mesh/C011991"},{"id":"T16","span":{"begin":79,"end":90},"obj":"http://purl.obolibrary.org/obo/CHEBI_26115"},{"id":"T17","span":{"begin":91,"end":102},"obj":"http://id.nlm.nih.gov/mesh/C099724"},{"id":"T18","span":{"begin":91,"end":102},"obj":"http://id.nlm.nih.gov/mesh/C099724"},{"id":"T19","span":{"begin":91,"end":102},"obj":"http://purl.obolibrary.org/obo/CHEBI_28927"},{"id":"T20","span":{"begin":108,"end":112},"obj":"http://id.nlm.nih.gov/mesh/D012275"},{"id":"T21","span":{"begin":108,"end":112},"obj":"http://purl.bioontology.org/ontology/RXNORM/1311040"},{"id":"T22","span":{"begin":108,"end":112},"obj":"http://www.ncbi.nlm.nih.gov/taxonomy/4530"},{"id":"T23","span":{"begin":114,"end":119},"obj":"http://id.nlm.nih.gov/mesh/D012275"},{"id":"T24","span":{"begin":114,"end":126},"obj":"http://www.ncbi.nlm.nih.gov/taxonomy/4530"},{"id":"T25","span":{"begin":114,"end":126},"obj":"http://id.nlm.nih.gov/mesh/D012275"},{"id":"T26","span":{"begin":145,"end":168},"obj":"http://id.nlm.nih.gov/mesh/D012436"},{"id":"T27","span":{"begin":145,"end":168},"obj":"http://purl.obolibrary.org/obo/CHEBI_15414"},{"id":"T28","span":{"begin":145,"end":168},"obj":"http://purl.obolibrary.org/obo/CHEBI_59789"},{"id":"T29","span":{"begin":145,"end":168},"obj":"http://id.nlm.nih.gov/mesh/D012436"},{"id":"T30","span":{"begin":147,"end":155},"obj":"http://purl.obolibrary.org/obo/CHEBI_33505"},{"id":"T31","span":{"begin":156,"end":168},"obj":"http://id.nlm.nih.gov/mesh/D008715"},{"id":"T32","span":{"begin":156,"end":168},"obj":"http://purl.obolibrary.org/obo/CHEBI_16044"},{"id":"T33","span":{"begin":156,"end":168},"obj":"http://purl.obolibrary.org/obo/CHEBI_16643"},{"id":"T34","span":{"begin":156,"end":168},"obj":"http://purl.bioontology.org/ontology/RXNORM/6837"},{"id":"T35","span":{"begin":156,"end":168},"obj":"http://purl.obolibrary.org/obo/CHEBI_49038"},{"id":"T36","span":{"begin":156,"end":168},"obj":"http://id.nlm.nih.gov/mesh/D008715"},{"id":"T37","span":{"begin":156,"end":168},"obj":"http://purl.obolibrary.org/obo/CHEBI_49037"},{"id":"T38","span":{"begin":156,"end":168},"obj":"http://purl.obolibrary.org/obo/CHEBI_57844"},{"id":"T39","span":{"begin":158,"end":168},"obj":"http://id.nlm.nih.gov/mesh/D008715"},{"id":"T40","span":{"begin":158,"end":168},"obj":"http://purl.obolibrary.org/obo/CHEBI_64558"},{"id":"T41","span":{"begin":158,"end":168},"obj":"http://purl.bioontology.org/ontology/RXNORM/6837"},{"id":"T42","span":{"begin":158,"end":168},"obj":"http://purl.obolibrary.org/obo/CHEBI_16643"},{"id":"T43","span":{"begin":158,"end":168},"obj":"http://id.nlm.nih.gov/mesh/D008715"},{"id":"T44","span":{"begin":158,"end":168},"obj":"http://purl.obolibrary.org/obo/SO_0001442"},{"id":"T45","span":{"begin":158,"end":168},"obj":"http://purl.obolibrary.org/obo/CHEBI_16811"},{"id":"T46","span":{"begin":169,"end":179},"obj":"http://purl.obolibrary.org/obo/CHEBI_50202"},{"id":"T47","span":{"begin":169,"end":179},"obj":"http://id.nlm.nih.gov/mesh/C005273"},{"id":"T48","span":{"begin":169,"end":179},"obj":"http://id.nlm.nih.gov/mesh/C005273"},{"id":"T49","span":{"begin":169,"end":179},"obj":"http://purl.obolibrary.org/obo/CHEBI_17846"},{"id":"T50","span":{"begin":169,"end":201},"obj":"http://id.nlm.nih.gov/mesh/C099725"},{"id":"T51","span":{"begin":169,"end":201},"obj":"NOMT"},{"id":"T52","span":{"begin":169,"end":201},"obj":"http://www.uniprot.org/uniprot/Q0IP69"},{"id":"T53","span":{"begin":203,"end":207},"obj":"http://www.uniprot.org/uniprot/Q0IP69"},{"id":"T54","span":{"begin":224,"end":235},"obj":"http://purl.obolibrary.org/obo/MOP_0000364"},{"id":"T55","span":{"begin":224,"end":235},"obj":"http://purl.obolibrary.org/obo/GO_0032259"},{"id":"T56","span":{"begin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