PubMed:10814825 JSON TXT

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Oryza-OGER

OryzaGP_2021_v2

{"project":"OryzaGP_2021_v2","denotations":[{"id":"T1","span":{"begin":203,"end":207},"obj":"http://identifiers.org/oryzabase.gene/10420"},{"id":"T2","span":{"begin":806,"end":809},"obj":"http://identifiers.org/oryzabase.gene/2454"},{"id":"T3","span":{"begin":840,"end":844},"obj":"http://identifiers.org/oryzabase.gene/10420"},{"id":"T4","span":{"begin":903,"end":905},"obj":"http://identifiers.org/oryzabase.gene/546"},{"id":"T5","span":{"begin":1044,"end":1048},"obj":"http://identifiers.org/oryzabase.gene/10420"},{"id":"T6","span":{"begin":1305,"end":1309},"obj":"http://identifiers.org/oryzabase.gene/10420"},{"id":"T55594","span":{"begin":203,"end":207},"obj":"http://identifiers.org/rapdb.locus/Os12g0240900"},{"id":"T92965","span":{"begin":840,"end":844},"obj":"http://identifiers.org/rapdb.locus/Os12g0240900"},{"id":"T95111","span":{"begin":1044,"end":1048},"obj":"http://identifiers.org/rapdb.locus/Os12g0240900"},{"id":"T27468","span":{"begin":1305,"end":1309},"obj":"http://identifiers.org/rapdb.locus/Os12g0240900"}],"text":"Naringenin 7-O-methyltransferase involved in the biosynthesis of the flavanone phytoalexin sakuranetin from rice (Oryza sativa L.).\nAn inducible S-adenosyl-L-methionine:naringenin 7-O-methyltransferase (NOMT) catalyzing the methylation of naringenin to sakuranetin, a major rice phytoalexin was purified approximately 985-fold from ultraviolet (UV)-irradiated rice leaves. The enzyme is not found in healthy tissues and was purified to a nearly homogeneous preparation in one step using adenosine-agarose affinity chromatography, with 1 g rice leaves (UV-irradiated) as starting material. Gel filtration chromatography resulted in an almost pure enzyme, as evidenced by a major band migrating to a position corresponding to a molecular mass of approximately 41 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The purified NOMT was strongly inhibited by Mn(2+), Ni(2+), Cu(2+), Zn(2+), Hg(2+), and Cd(2+), and to a low degree by Co(2+), Mg(2+), Ba(2+), Ca(2+) and ethylenediamine tetraacetic acid. The amino acid sequence of a NOMT cyanogen bromide (CNBr)-cleavage peptide was highly homologous to that of a caffeic acid 3-O-methyltransferase from maize, and about 70% of the amino acid sequence was obtained after sequencing the peptides generated by CNBr and/or formic acid hydrolysis. NOMT was also shown to be induced in a time-dependent manner, and purified from rice leaves treated with jasmonic acid and copper chloride."}

21k_plant_trait_mention

OryzaGP_2021

OryzaGP_2022

{"project":"OryzaGP_2022","denotations":[{"id":"T1","span":{"begin":0,"end":32},"obj":"http://identifiers.org/oryzabase.gene/10420"},{"id":"T2","span":{"begin":182,"end":201},"obj":"http://identifiers.org/oryzabase.gene/18240"},{"id":"T3","span":{"begin":182,"end":201},"obj":"http://identifiers.org/oryzabase.gene/16788"},{"id":"T4","span":{"begin":535,"end":536},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T5","span":{"begin":1140,"end":1159},"obj":"http://identifiers.org/oryzabase.gene/18240"},{"id":"T6","span":{"begin":1140,"end":1159},"obj":"http://identifiers.org/oryzabase.gene/16788"}],"text":"Naringenin 7-O-methyltransferase involved in the biosynthesis of the flavanone phytoalexin sakuranetin from rice (Oryza sativa L.).\nAn inducible S-adenosyl-L-methionine:naringenin 7-O-methyltransferase (NOMT) catalyzing the methylation of naringenin to sakuranetin, a major rice phytoalexin was purified approximately 985-fold from ultraviolet (UV)-irradiated rice leaves. The enzyme is not found in healthy tissues and was purified to a nearly homogeneous preparation in one step using adenosine-agarose affinity chromatography, with 1 g rice leaves (UV-irradiated) as starting material. Gel filtration chromatography resulted in an almost pure enzyme, as evidenced by a major band migrating to a position corresponding to a molecular mass of approximately 41 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The purified NOMT was strongly inhibited by Mn(2+), Ni(2+), Cu(2+), Zn(2+), Hg(2+), and Cd(2+), and to a low degree by Co(2+), Mg(2+), Ba(2+), Ca(2+) and ethylenediamine tetraacetic acid. The amino acid sequence of a NOMT cyanogen bromide (CNBr)-cleavage peptide was highly homologous to that of a caffeic acid 3-O-methyltransferase from maize, and about 70% of the amino acid sequence was obtained after sequencing the peptides generated by CNBr and/or formic acid hydrolysis. NOMT was also shown to be induced in a time-dependent manner, and purified from rice leaves treated with jasmonic acid and copper chloride."}

pqqtest_sentence

OryzaGP_2021_FLAIR