PubMed:10814696 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10814696","sourcedb":"PubMed","sourceid":"10814696","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10814696","text":"High-mannose-type oligosaccharides from human placental arylsulfatase A are core fucosylated as confirmed by MALDI MS.\nDespite numerous studies on arylsulfatase A, the structure of its glycans is not well understood. It has been shown that the concentration of arylsulfatase A increases in the body fluids of patients with some forms of cancer, and the carbohydrate component of arylsulfatase A synthesized in tumor tissues and transformed cells undergoes increased sialylation, phosphorylation and sulfation. To understand the significance of any changes in the glycosylation of arylsulfatase A in cancer, it is important to know the structure of its carbohydrate component in normal tissue. In the present study we have analyzed carbohydrate moieties of human placental arylsylfatase A using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by Western blotting on Immobilon P and on-blot deglycosylation using PNGase F for glycan release. Profiles of N-glycans were obtained by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Oligosaccharides were sequenced using specific exoglycosidases, and digestion products were analyzed by MALDI MS and the computer matching of the resulting masses with those derived from a sequence database. Fifty picomoles (6 microg) of arylsulfatase A applied to the gel were sufficient to characterize its oligosaccharide content. The results indicated that human placental arylsulfatase A possesses only high-mannose-type oligosaccharides, of which almost half are core fucosylated. In addition, there was a minor species of high-mannose-type glycan bearing six mannose residues with a core fucose. This structure was not expected since high-mannose-type oligosaccharides basically have not been recognized as a substrate for the alpha1,6-fucosyltransferase.","tracks":[{"project":"performance-test","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":416,"end":423},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"PD-UBERON-AE-B_T2","span":{"begin":685,"end":691},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"PD-UBERON-AE-B_T3","span":{"begin":46,"end":55},"obj":"http://purl.obolibrary.org/obo/UBERON_0001987"},{"id":"PD-UBERON-AE-B_T4","span":{"begin":762,"end":771},"obj":"http://purl.obolibrary.org/obo/UBERON_0001987"},{"id":"PD-UBERON-AE-B_T5","span":{"begin":1450,"end":1459},"obj":"http://purl.obolibrary.org/obo/UBERON_0001987"}],"attributes":[{"subj":"PD-UBERON-AE-B_T1","pred":"source","obj":"performance-test"},{"subj":"PD-UBERON-AE-B_T2","pred":"source","obj":"performance-test"},{"subj":"PD-UBERON-AE-B_T3","pred":"source","obj":"performance-test"},{"subj":"PD-UBERON-AE-B_T4","pred":"source","obj":"performance-test"},{"subj":"PD-UBERON-AE-B_T5","pred":"source","obj":"performance-test"}]},{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":337,"end":343},"obj":"HP_0002664"},{"id":"T2","span":{"begin":410,"end":415},"obj":"HP_0002664"},{"id":"T3","span":{"begin":599,"end":605},"obj":"HP_0002664"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"},{"subj":"T2","pred":"source","obj":"PubmedHPO"},{"subj":"T3","pred":"source","obj":"PubmedHPO"}]},{"project":"uniprot-human","denotations":[{"id":"T1","span":{"begin":115,"end":117},"obj":"http://www.uniprot.org/uniprot/Q99707"},{"id":"T2","span":{"begin":1078,"end":1080},"obj":"http://www.uniprot.org/uniprot/Q99707"},{"id":"T3","span":{"begin":1193,"end":1195},"obj":"http://www.uniprot.org/uniprot/Q99707"},{"id":"T4","span":{"begin":147,"end":162},"obj":"http://www.uniprot.org/uniprot/P15289"},{"id":"T5","span":{"begin":853,"end":856},"obj":"http://www.uniprot.org/uniprot/P20132"},{"id":"T6","span":{"begin":941,"end":947},"obj":"http://www.uniprot.org/uniprot/Q96IV0"}],"attributes":[{"subj":"T1","pred":"source","obj":"uniprot-human"},{"subj":"T2","pred":"source","obj":"uniprot-human"},{"subj":"T3","pred":"source","obj":"uniprot-human"},{"subj":"T4","pred":"source","obj":"uniprot-human"},{"subj":"T5","pred":"source","obj":"uniprot-human"},{"subj":"T6","pred":"source","obj":"uniprot-human"}]},{"project":"GlycoBiology-FMA","denotations":[{"id":"_T1","span":{"begin":5,"end":12},"obj":"FMAID:196796"},{"id":"_T2","span":{"begin":5,"end":12},"obj":"FMAID:82801"},{"id":"_T3","span":{"begin":18,"end":34},"obj":"FMAID:82742"},{"id":"_T4","span":{"begin":18,"end":34},"obj":"FMAID:196731"},{"id":"_T5","span":{"begin":294,"end":298},"obj":"FMAID:256135"},{"id":"_T6","span":{"begin":294,"end":298},"obj":"FMAID:256134"},{"id":"_T7","span":{"begin":294,"end":305},"obj":"FMAID:280617"},{"id":"_T8","span":{"begin":353,"end":365},"obj":"FMAID:197276"},{"id":"_T9","span":{"begin":353,"end":365},"obj":"FMAID:82737"},{"id":"_T10","span":{"begin":416,"end":423},"obj":"FMAID:256050"},{"id":"_T11","span":{"begin":440,"end":445},"obj":"FMAID:169002"},{"id":"_T12","span":{"begin":440,"end":445},"obj":"FMAID:68646"},{"id":"_T13","span":{"begin":652,"end":664},"obj":"FMAID:197276"},{"id":"_T14","span":{"begin":652,"end":664},"obj":"FMAID:82737"},{"id":"_T15","span":{"begin":685,"end":691},"obj":"FMAID:256050"},{"id":"_T16","span":{"begin":731,"end":743},"obj":"FMAID:197276"},{"id":"_T17","span":{"begin":731,"end":743},"obj":"FMAID:82737"},{"id":"_T18","span":{"begin":1083,"end":1099},"obj":"FMAID:82742"},{"id":"_T19","span":{"begin":1083,"end":1099},"obj":"FMAID:196731"},{"id":"_T20","span":{"begin":1392,"end":1407},"obj":"FMAID:196731"},{"id":"_T21","span":{"begin":1392,"end":1407},"obj":"FMAID:82742"},{"id":"_T22","span":{"begin":1496,"end":1503},"obj":"FMAID:196796"},{"id":"_T23","span":{"begin":1496,"end":1503},"obj":"FMAID:82801"},{"id":"_T24","span":{"begin":1509,"end":1525},"obj":"FMAID:82742"},{"id":"_T25","span":{"begin":1509,"end":1525},"obj":"FMAID:196731"},{"id":"_T26","span":{"begin":1617,"end":1624},"obj":"FMAID:82801"},{"id":"_T27","span":{"begin":1617,"end":1624},"obj":"FMAID:196796"},{"id":"_T28","span":{"begin":1649,"end":1656},"obj":"FMAID:82801"},{"id":"_T29","span":{"begin":1649,"end":1656},"obj":"FMAID:196796"},{"id":"_T30","span":{"begin":1678,"end":1684},"obj":"FMAID:196784"},{"id":"_T31","span":{"begin":1678,"end":1684},"obj":"FMAID:82790"},{"id":"_T32","span":{"begin":1729,"end":1736},"obj":"FMAID:82801"},{"id":"_T33","span":{"begin":1729,"end":1736},"obj":"FMAID:196796"},{"id":"_T34","span":{"begin":1742,"end":1758},"obj":"FMAID:82742"},{"id":"_T35","span":{"begin":1742,"end":1758},"obj":"FMAID:196731"}],"namespaces":[{"prefix":"FMAID","uri":"http://purl.org/sig/ont/fma/fma"}],"attributes":[{"subj":"_T1","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T2","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T3","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T4","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T5","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T6","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T7","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T8","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T9","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T10","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T11","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T12","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T13","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T14","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T15","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T16","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T17","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T18","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T19","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T20","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T21","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T22","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T23","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T24","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T25","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T26","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T27","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T28","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T29","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T30","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T31","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T32","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T33","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T34","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T35","pred":"source","obj":"GlycoBiology-FMA"}]},{"project":"uniprot-mouse","denotations":[{"id":"T1","span":{"begin":115,"end":117},"obj":"http://www.uniprot.org/uniprot/A6H5Y3"},{"id":"T2","span":{"begin":1078,"end":1080},"obj":"http://www.uniprot.org/uniprot/A6H5Y3"},{"id":"T3","span":{"begin":1193,"end":1195},"obj":"http://www.uniprot.org/uniprot/A6H5Y3"},{"id":"T4","span":{"begin":147,"end":162},"obj":"http://www.uniprot.org/uniprot/P50428"},{"id":"T5","span":{"begin":941,"end":947},"obj":"http://www.uniprot.org/uniprot/Q9JI78"}],"attributes":[{"subj":"T1","pred":"source","obj":"uniprot-mouse"},{"subj":"T2","pred":"source","obj":"uniprot-mouse"},{"subj":"T3","pred":"source","obj":"uniprot-mouse"},{"subj":"T4","pred":"source","obj":"uniprot-mouse"},{"subj":"T5","pred":"source","obj":"uniprot-mouse"}]},{"project":"GlycoBiology-NCBITAXON","denotations":[{"id":"T1","span":{"begin":337,"end":343},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/6754"},{"id":"T2","span":{"begin":416,"end":423},"obj":"http://purl.bioontology.org/ontology/STY/T024"},{"id":"T3","span":{"begin":440,"end":445},"obj":"http://purl.bioontology.org/ontology/STY/T025"},{"id":"T4","span":{"begin":599,"end":605},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/6754"},{"id":"T5","span":{"begin":685,"end":691},"obj":"http://purl.bioontology.org/ontology/STY/T024"},{"id":"T6","span":{"begin":1025,"end":1030},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/62990"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T2","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T3","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T4","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T5","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T6","pred":"source","obj":"GlycoBiology-NCBITAXON"}]},{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":118},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":119,"end":216},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":217,"end":509},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":510,"end":692},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":693,"end":969},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":970,"end":1082},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":1083,"end":1290},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1291,"end":1416},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1417,"end":1569},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":1570,"end":1685},"obj":"Sentence"},{"id":"TextSentencer_T11","span":{"begin":1686,"end":1845},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":118},"obj":"Sentence"},{"id":"T2","span":{"begin":119,"end":216},"obj":"Sentence"},{"id":"T3","span":{"begin":217,"end":509},"obj":"Sentence"},{"id":"T4","span":{"begin":510,"end":692},"obj":"Sentence"},{"id":"T5","span":{"begin":693,"end":969},"obj":"Sentence"},{"id":"T6","span":{"begin":970,"end":1082},"obj":"Sentence"},{"id":"T7","span":{"begin":1083,"end":1290},"obj":"Sentence"},{"id":"T8","span":{"begin":1291,"end":1416},"obj":"Sentence"},{"id":"T9","span":{"begin":1417,"end":1569},"obj":"Sentence"},{"id":"T10","span":{"begin":1570,"end":1685},"obj":"Sentence"},{"id":"T11","span":{"begin":1686,"end":1845},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":118},"obj":"Sentence"},{"id":"T2","span":{"begin":119,"end":216},"obj":"Sentence"},{"id":"T3","span":{"begin":217,"end":509},"obj":"Sentence"},{"id":"T4","span":{"begin":510,"end":692},"obj":"Sentence"},{"id":"T5","span":{"begin":693,"end":969},"obj":"Sentence"},{"id":"T6","span":{"begin":970,"end":1082},"obj":"Sentence"},{"id":"T7","span":{"begin":1083,"end":1290},"obj":"Sentence"},{"id":"T8","span":{"begin":1291,"end":1416},"obj":"Sentence"},{"id":"T9","span":{"begin":1417,"end":1569},"obj":"Sentence"},{"id":"T10","span":{"begin":1570,"end":1685},"obj":"Sentence"},{"id":"T11","span":{"begin":1686,"end":1845},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T9","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T10","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T11","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"},{"subj":"T11","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"},{"subj":"T11","pred":"source","obj":"sentences"}]},{"project":"GO-BP","denotations":[{"id":"T1","span":{"begin":46,"end":55},"obj":"http://purl.obolibrary.org/obo/GO_0001890"},{"id":"T2","span":{"begin":762,"end":771},"obj":"http://purl.obolibrary.org/obo/GO_0001890"},{"id":"T3","span":{"begin":1450,"end":1459},"obj":"http://purl.obolibrary.org/obo/GO_0001890"},{"id":"T4","span":{"begin":81,"end":92},"obj":"http://purl.obolibrary.org/obo/GO_0036065"},{"id":"T5","span":{"begin":1557,"end":1568},"obj":"http://purl.obolibrary.org/obo/GO_0036065"},{"id":"T6","span":{"begin":466,"end":477},"obj":"http://purl.obolibrary.org/obo/GO_0097503"},{"id":"T7","span":{"begin":479,"end":494},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T8","span":{"begin":499,"end":508},"obj":"http://purl.obolibrary.org/obo/GO_0051923"},{"id":"T9","span":{"begin":809,"end":816},"obj":"http://purl.obolibrary.org/obo/GO_0051923"},{"id":"T10","span":{"begin":563,"end":576},"obj":"http://purl.obolibrary.org/obo/GO_0070085"},{"id":"T11","span":{"begin":941,"end":947},"obj":"http://purl.obolibrary.org/obo/GO_0000224"},{"id":"T12","span":{"begin":941,"end":949},"obj":"http://purl.obolibrary.org/obo/GO_0000224"},{"id":"T13","span":{"begin":1151,"end":1160},"obj":"http://purl.obolibrary.org/obo/GO_0007586"}],"attributes":[{"subj":"T1","pred":"source","obj":"GO-BP"},{"subj":"T2","pred":"source","obj":"GO-BP"},{"subj":"T3","pred":"source","obj":"GO-BP"},{"subj":"T4","pred":"source","obj":"GO-BP"},{"subj":"T5","pred":"source","obj":"GO-BP"},{"subj":"T6","pred":"source","obj":"GO-BP"},{"subj":"T7","pred":"source","obj":"GO-BP"},{"subj":"T8","pred":"source","obj":"GO-BP"},{"subj":"T9","pred":"source","obj":"GO-BP"},{"subj":"T10","pred":"source","obj":"GO-BP"},{"subj":"T11","pred":"source","obj":"GO-BP"},{"subj":"T12","pred":"source","obj":"GO-BP"},{"subj":"T13","pred":"source","obj":"GO-BP"}]},{"project":"GO-CC","denotations":[{"id":"T1","span":{"begin":76,"end":80},"obj":"http://purl.obolibrary.org/obo/GO_0019013"},{"id":"T2","span":{"begin":1552,"end":1556},"obj":"http://purl.obolibrary.org/obo/GO_0019013"},{"id":"T3","span":{"begin":1673,"end":1677},"obj":"http://purl.obolibrary.org/obo/GO_0019013"},{"id":"T4","span":{"begin":440,"end":445},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"attributes":[{"subj":"T1","pred":"source","obj":"GO-CC"},{"subj":"T2","pred":"source","obj":"GO-CC"},{"subj":"T3","pred":"source","obj":"GO-CC"},{"subj":"T4","pred":"source","obj":"GO-CC"}]},{"project":"UBERON-AE","denotations":[{"id":"T1","span":{"begin":416,"end":423},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T2","span":{"begin":685,"end":691},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"}],"attributes":[{"subj":"T1","pred":"source","obj":"UBERON-AE"},{"subj":"T2","pred":"source","obj":"UBERON-AE"}]},{"project":"EDAM-topics","denotations":[{"id":"T1","span":{"begin":40,"end":45},"obj":"http://edamontology.org/topic_2815"},{"id":"T2","span":{"begin":136,"end":143},"obj":"http://edamontology.org/topic_3678"},{"id":"T3","span":{"begin":337,"end":343},"obj":"http://edamontology.org/topic_2640"},{"id":"T4","span":{"begin":353,"end":365},"obj":"http://edamontology.org/topic_0152"},{"id":"T5","span":{"begin":599,"end":605},"obj":"http://edamontology.org/topic_2640"},{"id":"T6","span":{"begin":652,"end":664},"obj":"http://edamontology.org/topic_0152"},{"id":"T7","span":{"begin":708,"end":713},"obj":"http://edamontology.org/topic_3678"},{"id":"T8","span":{"begin":731,"end":743},"obj":"http://edamontology.org/topic_0152"},{"id":"T9","span":{"begin":756,"end":761},"obj":"http://edamontology.org/topic_2815"},{"id":"T10","span":{"begin":1053,"end":1070},"obj":"http://edamontology.org/topic_0134"},{"id":"T11","span":{"begin":1053,"end":1070},"obj":"http://edamontology.org/topic_3520"},{"id":"T12","span":{"begin":1105,"end":1114},"obj":"http://edamontology.org/topic_3168"},{"id":"T13","span":{"begin":1105,"end":1114},"obj":"http://edamontology.org/topic_0080"},{"id":"T14","span":{"begin":1272,"end":1280},"obj":"http://edamontology.org/topic_3168"},{"id":"T15","span":{"begin":1272,"end":1280},"obj":"http://edamontology.org/topic_0080"},{"id":"T16","span":{"begin":1272,"end":1289},"obj":"http://edamontology.org/topic_3041"},{"id":"T17","span":{"begin":1272,"end":1289},"obj":"http://edamontology.org/topic_0080"},{"id":"T18","span":{"begin":1281,"end":1289},"obj":"http://edamontology.org/topic_3489"},{"id":"T19","span":{"begin":1444,"end":1449},"obj":"http://edamontology.org/topic_2815"}],"attributes":[{"subj":"T1","pred":"source","obj":"EDAM-topics"},{"subj":"T2","pred":"source","obj":"EDAM-topics"},{"subj":"T3","pred":"source","obj":"EDAM-topics"},{"subj":"T4","pred":"source","obj":"EDAM-topics"},{"subj":"T5","pred":"source","obj":"EDAM-topics"},{"subj":"T6","pred":"source","obj":"EDAM-topics"},{"subj":"T7","pred":"source","obj":"EDAM-topics"},{"subj":"T8","pred":"source","obj":"EDAM-topics"},{"subj":"T9","pred":"source","obj":"EDAM-topics"},{"subj":"T10","pred":"source","obj":"EDAM-topics"},{"subj":"T11","pred":"source","obj":"EDAM-topics"},{"subj":"T12","pred":"source","obj":"EDAM-topics"},{"subj":"T13","pred":"source","obj":"EDAM-topics"},{"subj":"T14","pred":"source","obj":"EDAM-topics"},{"subj":"T15","pred":"source","obj":"EDAM-topics"},{"subj":"T16","pred":"source","obj":"EDAM-topics"},{"subj":"T17","pred":"source","obj":"EDAM-topics"},{"subj":"T18","pred":"source","obj":"EDAM-topics"},{"subj":"T19","pred":"source","obj":"EDAM-topics"}]},{"project":"EDAM-DFO","denotations":[{"id":"T1","span":{"begin":168,"end":177},"obj":"http://edamontology.org/data_0883"},{"id":"T2","span":{"begin":244,"end":257},"obj":"http://edamontology.org/data_2140"},{"id":"T3","span":{"begin":635,"end":644},"obj":"http://edamontology.org/data_0883"},{"id":"T4","span":{"begin":678,"end":684},"obj":"http://edamontology.org/operation_3435"},{"id":"T5","span":{"begin":1009,"end":1015},"obj":"http://edamontology.org/data_2082"},{"id":"T6","span":{"begin":1053,"end":1070},"obj":"http://edamontology.org/data_2536"},{"id":"T7","span":{"begin":1053,"end":1070},"obj":"http://edamontology.org/data_3147"},{"id":"T8","span":{"begin":1105,"end":1114},"obj":"http://edamontology.org/data_2044"},{"id":"T9","span":{"begin":1105,"end":1114},"obj":"http://edamontology.org/operation_3218"},{"id":"T10","span":{"begin":1213,"end":1221},"obj":"http://edamontology.org/format_1990"},{"id":"T11","span":{"begin":1272,"end":1280},"obj":"http://edamontology.org/data_2044"},{"id":"T12","span":{"begin":1272,"end":1280},"obj":"http://edamontology.org/operation_3218"},{"id":"T13","span":{"begin":1272,"end":1289},"obj":"http://edamontology.org/data_1057"},{"id":"T14","span":{"begin":1272,"end":1289},"obj":"http://edamontology.org/data_0857"},{"id":"T15","span":{"begin":1272,"end":1289},"obj":"http://edamontology.org/data_2925"},{"id":"T16","span":{"begin":1281,"end":1289},"obj":"http://edamontology.org/data_0581"},{"id":"T17","span":{"begin":1657,"end":1665},"obj":"http://edamontology.org/data_1756"},{"id":"T18","span":{"begin":1691,"end":1700},"obj":"http://edamontology.org/data_0883"}],"attributes":[{"subj":"T1","pred":"source","obj":"EDAM-DFO"},{"subj":"T2","pred":"source","obj":"EDAM-DFO"},{"subj":"T3","pred":"source","obj":"EDAM-DFO"},{"subj":"T4","pred":"source","obj":"EDAM-DFO"},{"subj":"T5","pred":"source","obj":"EDAM-DFO"},{"subj":"T6","pred":"source","obj":"EDAM-DFO"},{"subj":"T7","pred":"source","obj":"EDAM-DFO"},{"subj":"T8","pred":"source","obj":"EDAM-DFO"},{"subj":"T9","pred":"source","obj":"EDAM-DFO"},{"subj":"T10","pred":"source","obj":"EDAM-DFO"},{"subj":"T11","pred":"source","obj":"EDAM-DFO"},{"subj":"T12","pred":"source","obj":"EDAM-DFO"},{"subj":"T13","pred":"source","obj":"EDAM-DFO"},{"subj":"T14","pred":"source","obj":"EDAM-DFO"},{"subj":"T15","pred":"source","obj":"EDAM-DFO"},{"subj":"T16","pred":"source","obj":"EDAM-DFO"},{"subj":"T17","pred":"source","obj":"EDAM-DFO"},{"subj":"T18","pred":"source","obj":"EDAM-DFO"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":40,"end":45},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":756,"end":761},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":1444,"end":1449},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9606"},{"id":"A3","pred":"db_id","subj":"T3","obj":"9606"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"},{"subj":"T3","pred":"source","obj":"NCBITAXON"}]},{"project":"GlycoBiology-Motifs","denotations":[{"id":"T1","span":{"begin":0,"end":12},"obj":"http://rdf.glycoinfo.org/glycan/G00028MO"},{"id":"T2","span":{"begin":1491,"end":1503},"obj":"http://rdf.glycoinfo.org/glycan/G00028MO"},{"id":"T3","span":{"begin":1612,"end":1624},"obj":"http://rdf.glycoinfo.org/glycan/G00028MO"},{"id":"T4","span":{"begin":1724,"end":1736},"obj":"http://rdf.glycoinfo.org/glycan/G00028MO"},{"id":"T5","span":{"begin":982,"end":991},"obj":"http://rdf.glycoinfo.org/glycan/G00027MO"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlycoBiology-Motifs"},{"subj":"T2","pred":"source","obj":"GlycoBiology-Motifs"},{"subj":"T3","pred":"source","obj":"GlycoBiology-Motifs"},{"subj":"T4","pred":"source","obj":"GlycoBiology-Motifs"},{"subj":"T5","pred":"source","obj":"GlycoBiology-Motifs"}]},{"project":"NGLY1-deficiency","denotations":[{"id":"PD-NGLY1-deficiency-B_T1","span":{"begin":941,"end":947},"obj":"hgnc:17646"}],"namespaces":[{"prefix":"hgnc","uri":"https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:"},{"prefix":"omim","uri":"https://www.omim.org/entry/"},{"prefix":"chem","uri":"https://pubchem.ncbi.nlm.nih.gov/compound/"}],"attributes":[{"subj":"PD-NGLY1-deficiency-B_T1","pred":"source","obj":"NGLY1-deficiency"}]},{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":337,"end":343},"obj":"Disease"},{"id":"T2","span":{"begin":410,"end":415},"obj":"Disease"},{"id":"T3","span":{"begin":599,"end":605},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0004992"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0005070"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0004992"},{"subj":"T1","pred":"source","obj":"mondo_disease"},{"subj":"T2","pred":"source","obj":"mondo_disease"},{"subj":"T3","pred":"source","obj":"mondo_disease"}]},{"project":"Glycan-Motif","denotations":[{"id":"T1","span":{"begin":5,"end":12},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"},{"id":"T2","span":{"begin":1496,"end":1503},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"},{"id":"T3","span":{"begin":1617,"end":1624},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"},{"id":"T4","span":{"begin":1649,"end":1656},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"},{"id":"T5","span":{"begin":1678,"end":1684},"obj":"https://glytoucan.org/Structures/Glycans/G82576YO"},{"id":"T6","span":{"begin":1729,"end":1736},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"}],"attributes":[{"subj":"T1","pred":"source","obj":"Glycan-Motif"},{"subj":"T2","pred":"source","obj":"Glycan-Motif"},{"subj":"T3","pred":"source","obj":"Glycan-Motif"},{"subj":"T4","pred":"source","obj":"Glycan-Motif"},{"subj":"T5","pred":"source","obj":"Glycan-Motif"},{"subj":"T6","pred":"source","obj":"Glycan-Motif"}]},{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":5,"end":12},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"},{"id":"T2","span":{"begin":1496,"end":1503},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"},{"id":"T3","span":{"begin":1617,"end":1624},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"},{"id":"T4","span":{"begin":1649,"end":1656},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"},{"id":"T5","span":{"begin":1678,"end":1684},"obj":"https://glytoucan.org/Structures/Glycans/G82576YO"},{"id":"T6","span":{"begin":1729,"end":1736},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T4","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T5","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T6","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"}]},{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":5,"end":12},"obj":"Glycan_Motif"},{"id":"T2","span":{"begin":1496,"end":1503},"obj":"Glycan_Motif"},{"id":"T3","span":{"begin":1617,"end":1624},"obj":"Glycan_Motif"},{"id":"T4","span":{"begin":1649,"end":1656},"obj":"Glycan_Motif"},{"id":"T5","span":{"begin":1678,"end":1684},"obj":"Glycan_Motif"},{"id":"T6","span":{"begin":1729,"end":1736},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ"},{"id":"A2","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ"},{"id":"A3","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ"},{"id":"A4","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ"},{"id":"A5","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO"},{"id":"A6","pred":"image","subj":"T6","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ"},{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T4","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T5","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T6","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":294,"end":305},"obj":"Body_part"},{"id":"T2","span":{"begin":685,"end":691},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0006314"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T2","pred":"source","obj":"Anatomy-UBERON"}]},{"project":"HP-phenotype","denotations":[{"id":"T1","span":{"begin":337,"end":343},"obj":"Phenotype"},{"id":"T2","span":{"begin":410,"end":415},"obj":"Phenotype"},{"id":"T3","span":{"begin":599,"end":605},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0002664"},{"id":"A2","pred":"hp_id","subj":"T2","obj":"HP:0002664"},{"id":"A3","pred":"hp_id","subj":"T3","obj":"HP:0002664"},{"subj":"T1","pred":"source","obj":"HP-phenotype"},{"subj":"T2","pred":"source","obj":"HP-phenotype"},{"subj":"T3","pred":"source","obj":"HP-phenotype"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"performance-test","color":"#e793ec","default":true},{"id":"PubmedHPO","color":"#93eccd"},{"id":"uniprot-human","color":"#ecb393"},{"id":"GlycoBiology-FMA","color":"#9993ec"},{"id":"uniprot-mouse","color":"#a6ec93"},{"id":"GlycoBiology-NCBITAXON","color":"#ec93c0"},{"id":"sentences","color":"#93daec"},{"id":"GO-BP","color":"#ece493"},{"id":"GO-CC","color":"#ca93ec"},{"id":"UBERON-AE","color":"#93ecaf"},{"id":"EDAM-topics","color":"#ec9593"},{"id":"EDAM-DFO","color":"#93aaec"},{"id":"NCBITAXON","color":"#c4ec93"},{"id":"GlycoBiology-Motifs","color":"#ec93de"},{"id":"NGLY1-deficiency","color":"#93ece0"},{"id":"mondo_disease","color":"#ecc693"},{"id":"Glycan-Motif","color":"#ac93ec"},{"id":"GlyCosmos6-Glycan-Motif-Structure","color":"#94ec93"},{"id":"GlyCosmos6-Glycan-Motif-Image","color":"#ec93ae"},{"id":"Anatomy-UBERON","color":"#93c8ec"},{"id":"HP-phenotype","color":"#e2ec93"}]}]}}