PubMed:10613883 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10613883","sourcedb":"PubMed","sourceid":"10613883","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10613883","text":"Uptake of 2-oxoglutarate in Synechococcus strains transformed with the Escherichia coli kgtP gene.\nA number of cyanobacteria from different taxonomic groups exhibited very low levels of uptake of 2-[U-(14)C]oxoglutarate. Synechococcus sp. strain PCC 7942 was transformed with DNA constructs carrying the Escherichia coli kgtP gene encoding a 2-oxoglutarate permease and a kanamycin resistance gene cassette. The Synechococcus sp. strains bearing the kgtP gene incorporated 2-oxoglutarate into the cells through an active transport process. About 75% of the radioactivity from the 2-[U-(14)C]oxoglutarate taken up that was recovered in soluble metabolites was found as glutamate and glutamine. 2-Oxoglutarate was, however, detrimental to the growth of a Synechococcus sp. strain bearing the kgtP gene.","tracks":[{"project":"PMID_GLOBAL","denotations":[{"id":"T1","span":{"begin":246,"end":249},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"0004974"},{"id":"A2","pred":"mondo_id","subj":"T1","obj":"0022673"},{"subj":"T1","pred":"source","obj":"PMID_GLOBAL"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PMID_GLOBAL","color":"#ec93bd","default":true}]}]}}