PubMed:10613883 JSONTXT

{"project":"PMID_GLOBAL","denotations":[{"id":"T1","span":{"begin":246,"end":249},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"0004974"},{"id":"A2","pred":"mondo_id","subj":"T1","obj":"0022673"}],"text":"Uptake of 2-oxoglutarate in Synechococcus strains transformed with the Escherichia coli kgtP gene.\nA number of cyanobacteria from different taxonomic groups exhibited very low levels of uptake of 2-[U-(14)C]oxoglutarate. Synechococcus sp. strain PCC 7942 was transformed with DNA constructs carrying the Escherichia coli kgtP gene encoding a 2-oxoglutarate permease and a kanamycin resistance gene cassette. The Synechococcus sp. strains bearing the kgtP gene incorporated 2-oxoglutarate into the cells through an active transport process. About 75% of the radioactivity from the 2-[U-(14)C]oxoglutarate taken up that was recovered in soluble metabolites was found as glutamate and glutamine. 2-Oxoglutarate was, however, detrimental to the growth of a Synechococcus sp. strain bearing the kgtP gene."}