PubMed:10610624 JSONTXT

{"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T1","span":{"begin":102,"end":389},"obj":"BACKGROUND"},{"id":"T2","span":{"begin":401,"end":577},"obj":"OBJECTIVE"},{"id":"T3","span":{"begin":586,"end":605},"obj":"METHODS"},{"id":"T4","span":{"begin":615,"end":653},"obj":"METHODS"},{"id":"T5","span":{"begin":664,"end":998},"obj":"METHODS"},{"id":"T6","span":{"begin":1013,"end":1191},"obj":"METHODS"},{"id":"T7","span":{"begin":1201,"end":1759},"obj":"RESULTS"},{"id":"T8","span":{"begin":1773,"end":2011},"obj":"CONCLUSIONS"}],"text":"Guanylyl cyclase C messenger RNA is a biomarker for recurrent stage II colorectal cancer.\nBACKGROUND: Patients with stage II colorectal cancer and no histologic evidence of lymph node invasion develop recurrent disease, presumably because of undetected micrometastases. Guanylyl cyclase C is expressed by intestinal and colorectal cancer cells but not by extraintestinal tissues or tumors.\nOBJECTIVE: To examine the expression of guanylyl cyclase C messenger RNA (mRNA) in lymph nodes of patients with node-negative colorectal cancer who did and did not have recurrent disease.\nDESIGN: Case-control study.\nSETTING: Tertiary care academic medical center.\nPATIENTS: Paraffin-embedded lymph nodes were obtained from 21 patients with histologically confirmed node-negative colorectal cancer who had undergone resection. Controls included 11 patients without disease recurrence 6 or more years after resection, and case-patients included 10 patients whose disease recurred up to 3 years after resection.\nMEASUREMENTS: Sections of paraffin-embedded lymph nodes were obtained from each patient and were pooled, and their RNA was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR).\nRESULTS: Guanylyl cyclase C mRNA was expressed in lymph nodes from all patients with recurrent disease but not in those from patients without recurrent disease (P = 0.004). Nested RT-PCR that used primers for carcinoembryonic antigen, a marker for colorectal cancer, identified carcinoembryonic antigen mRNA in lymph nodes from only 1 of 10 patients with recurrent disease and those from 0 of 11 patients without recurrent disease. The odds ratio for death associated with expression of guanylyl cyclase C mRNA in regional lymph nodes was 15.0 (95% CI, 1.1 to 756.7).\nCONCLUSIONS: Expression of guanylyl cyclase C mRNA in lymph nodes is associated with recurrence of colorectal cancer in patients with stage II disease. Analysis of guanylyl cyclase mRNA expression by RT-PCR may be useful for colorectal cancer staging."}

{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":136,"end":142},"obj":"HP_0002664"},{"id":"T2","span":{"begin":331,"end":337},"obj":"HP_0002664"},{"id":"T3","span":{"begin":382,"end":388},"obj":"HP_0002664"}],"text":"Guanylyl cyclase C messenger RNA is a biomarker for recurrent stage II colorectal cancer.\nBACKGROUND: Patients with stage II colorectal cancer and no histologic evidence of lymph node invasion develop recurrent disease, presumably because of undetected micrometastases. Guanylyl cyclase C is expressed by intestinal and colorectal cancer cells but not by extraintestinal tissues or tumors.\nOBJECTIVE: To examine the expression of guanylyl cyclase C messenger RNA (mRNA) in lymph nodes of patients with node-negative colorectal cancer who did and did not have recurrent disease.\nDESIGN: Case-control study.\nSETTING: Tertiary care academic medical center.\nPATIENTS: Paraffin-embedded lymph nodes were obtained from 21 patients with histologically confirmed node-negative colorectal cancer who had undergone resection. Controls included 11 patients without disease recurrence 6 or more years after resection, and case-patients included 10 patients whose disease recurred up to 3 years after resection.\nMEASUREMENTS: Sections of paraffin-embedded lymph nodes were obtained from each patient and were pooled, and their RNA was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR).\nRESULTS: Guanylyl cyclase C mRNA was expressed in lymph nodes from all patients with recurrent disease but not in those from patients without recurrent disease (P = 0.004). Nested RT-PCR that used primers for carcinoembryonic antigen, a marker for colorectal cancer, identified carcinoembryonic antigen mRNA in lymph nodes from only 1 of 10 patients with recurrent disease and those from 0 of 11 patients without recurrent disease. The odds ratio for death associated with expression of guanylyl cyclase C mRNA in regional lymph nodes was 15.0 (95% CI, 1.1 to 756.7).\nCONCLUSIONS: Expression of guanylyl cyclase C mRNA in lymph nodes is associated with recurrence of colorectal cancer in patients with stage II disease. Analysis of guanylyl cyclase mRNA expression by RT-PCR may be useful for colorectal cancer staging."}

{"project":"DisGeNET5_gene_disease","denotations":[{"id":"10610624-0#0#18#gene2984","span":{"begin":270,"end":288},"obj":"gene2984"},{"id":"10610624-0#62#88#diseaseC0677948","span":{"begin":1559,"end":1876},"obj":"diseaseC0677948"},{"id":"10610624-0#62#88#diseaseC3146252","span":{"begin":1559,"end":1876},"obj":"diseaseC3146252"},{"id":"10610624-10#36#60#gene1048","span":{"begin":1401,"end":1425},"obj":"gene1048"},{"id":"10610624-10#75#92#diseaseC0009402","span":{"begin":1440,"end":1457},"obj":"diseaseC0009402"},{"id":"10610624-10#75#92#diseaseC1527249","span":{"begin":1440,"end":1457},"obj":"diseaseC1527249"},{"id":"10610624-12#14#32#gene2984","span":{"begin":1787,"end":1805},"obj":"gene2984"},{"id":"10610624-12#86#103#diseaseC0009402","span":{"begin":1859,"end":1876},"obj":"diseaseC0009402"},{"id":"10610624-12#86#103#diseaseC1527249","span":{"begin":1859,"end":1876},"obj":"diseaseC1527249"}],"relations":[{"id":"0#18#gene298462#88#diseaseC0677948","pred":"associated_with","subj":"10610624-0#0#18#gene2984","obj":"10610624-0#62#88#diseaseC0677948"},{"id":"0#18#gene298462#88#diseaseC3146252","pred":"associated_with","subj":"10610624-0#0#18#gene2984","obj":"10610624-0#62#88#diseaseC3146252"},{"id":"36#60#gene104875#92#diseaseC0009402","pred":"associated_with","subj":"10610624-10#36#60#gene1048","obj":"10610624-10#75#92#diseaseC0009402"},{"id":"36#60#gene104875#92#diseaseC1527249","pred":"associated_with","subj":"10610624-10#36#60#gene1048","obj":"10610624-10#75#92#diseaseC1527249"},{"id":"14#32#gene298486#103#diseaseC0009402","pred":"associated_with","subj":"10610624-12#14#32#gene2984","obj":"10610624-12#86#103#diseaseC0009402"},{"id":"14#32#gene298486#103#diseaseC1527249","pred":"associated_with","subj":"10610624-12#14#32#gene2984","obj":"10610624-12#86#103#diseaseC1527249"}],"text":"Guanylyl cyclase C messenger RNA is a biomarker for recurrent stage II colorectal cancer.\nBACKGROUND: Patients with stage II colorectal cancer and no histologic evidence of lymph node invasion develop recurrent disease, presumably because of undetected micrometastases. Guanylyl cyclase C is expressed by intestinal and colorectal cancer cells but not by extraintestinal tissues or tumors.\nOBJECTIVE: To examine the expression of guanylyl cyclase C messenger RNA (mRNA) in lymph nodes of patients with node-negative colorectal cancer who did and did not have recurrent disease.\nDESIGN: Case-control study.\nSETTING: Tertiary care academic medical center.\nPATIENTS: Paraffin-embedded lymph nodes were obtained from 21 patients with histologically confirmed node-negative colorectal cancer who had undergone resection. Controls included 11 patients without disease recurrence 6 or more years after resection, and case-patients included 10 patients whose disease recurred up to 3 years after resection.\nMEASUREMENTS: Sections of paraffin-embedded lymph nodes were obtained from each patient and were pooled, and their RNA was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR).\nRESULTS: Guanylyl cyclase C mRNA was expressed in lymph nodes from all patients with recurrent disease but not in those from patients without recurrent disease (P = 0.004). Nested RT-PCR that used primers for carcinoembryonic antigen, a marker for colorectal cancer, identified carcinoembryonic antigen mRNA in lymph nodes from only 1 of 10 patients with recurrent disease and those from 0 of 11 patients without recurrent disease. The odds ratio for death associated with expression of guanylyl cyclase C mRNA in regional lymph nodes was 15.0 (95% CI, 1.1 to 756.7).\nCONCLUSIONS: Expression of guanylyl cyclase C mRNA in lymph nodes is associated with recurrence of colorectal cancer in patients with stage II disease. Analysis of guanylyl cyclase mRNA expression by RT-PCR may be useful for colorectal cancer staging."}

{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":270,"end":288},"obj":"gene:2984"},{"id":"T1","span":{"begin":320,"end":337},"obj":"disease:C0009402"},{"id":"T2","span":{"begin":270,"end":288},"obj":"gene:2984"},{"id":"T3","span":{"begin":320,"end":337},"obj":"disease:C1527249"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"text":"Guanylyl cyclase C messenger RNA is a biomarker for recurrent stage II colorectal cancer.\nBACKGROUND: Patients with stage II colorectal cancer and no histologic evidence of lymph node invasion develop recurrent disease, presumably because of undetected micrometastases. Guanylyl cyclase C is expressed by intestinal and colorectal cancer cells but not by extraintestinal tissues or tumors.\nOBJECTIVE: To examine the expression of guanylyl cyclase C messenger RNA (mRNA) in lymph nodes of patients with node-negative colorectal cancer who did and did not have recurrent disease.\nDESIGN: Case-control study.\nSETTING: Tertiary care academic medical center.\nPATIENTS: Paraffin-embedded lymph nodes were obtained from 21 patients with histologically confirmed node-negative colorectal cancer who had undergone resection. Controls included 11 patients without disease recurrence 6 or more years after resection, and case-patients included 10 patients whose disease recurred up to 3 years after resection.\nMEASUREMENTS: Sections of paraffin-embedded lymph nodes were obtained from each patient and were pooled, and their RNA was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR).\nRESULTS: Guanylyl cyclase C mRNA was expressed in lymph nodes from all patients with recurrent disease but not in those from patients without recurrent disease (P = 0.004). Nested RT-PCR that used primers for carcinoembryonic antigen, a marker for colorectal cancer, identified carcinoembryonic antigen mRNA in lymph nodes from only 1 of 10 patients with recurrent disease and those from 0 of 11 patients without recurrent disease. The odds ratio for death associated with expression of guanylyl cyclase C mRNA in regional lymph nodes was 15.0 (95% CI, 1.1 to 756.7).\nCONCLUSIONS: Expression of guanylyl cyclase C mRNA in lymph nodes is associated with recurrence of colorectal cancer in patients with stage II disease. Analysis of guanylyl cyclase mRNA expression by RT-PCR may be useful for colorectal cancer staging."}