PubMed:10523842 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10523842","sourcedb":"PubMed","sourceid":"10523842","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10523842","text":"Role of two upstream open reading frames in the translational control of oncogene mdm2.\nOverexpression of oncoprotein MDM2 has been found in a significant number of human soft tissue tumors. In a subset of these tumors, overexpression is a result of enhanced translation of mdm2 mRNA. There are two transcripts from the mdm2 gene that differ only in their 5' leaders: a long form (L-mdm2) and a short form (S-mdm2) that arise from the use of different promoters. L-mdm2 mRNA contains two upstream open reading frames (uORFs) and this mRNA was loaded with ribosomes inefficiently in comparison with S-mdm2. The 5' leader of L-mdm2 was sufficient to transfer translational repression to a reporter gene and the two uORFs acted synergistically to achieve full suppression. In contrast, the 5' leader of S-mdm2 allowed efficient translation of an attached reporter gene in the tumor cells. These results are consistent with a model in which overexpression of MDM2 in certain tumors results from a change in mRNA structure due to a switch in promoter usage.","tracks":[{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":183,"end":189},"obj":"HP_0002664"},{"id":"T2","span":{"begin":212,"end":218},"obj":"HP_0002664"},{"id":"T3","span":{"begin":873,"end":878},"obj":"HP_0002664"},{"id":"T4","span":{"begin":971,"end":977},"obj":"HP_0002664"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"},{"subj":"T2","pred":"source","obj":"PubmedHPO"},{"subj":"T3","pred":"source","obj":"PubmedHPO"},{"subj":"T4","pred":"source","obj":"PubmedHPO"}]},{"project":"bionlp-st-pc-2013-training","denotations":[{"id":"T1","span":{"begin":82,"end":86},"obj":"Gene_or_gene_product"},{"id":"T2","span":{"begin":118,"end":122},"obj":"Gene_or_gene_product"},{"id":"T3","span":{"begin":274,"end":278},"obj":"Gene_or_gene_product"},{"id":"T4","span":{"begin":320,"end":324},"obj":"Gene_or_gene_product"},{"id":"T5","span":{"begin":381,"end":387},"obj":"Gene_or_gene_product"},{"id":"T6","span":{"begin":407,"end":413},"obj":"Gene_or_gene_product"},{"id":"T7","span":{"begin":463,"end":469},"obj":"Gene_or_gene_product"},{"id":"T8","span":{"begin":598,"end":604},"obj":"Gene_or_gene_product"},{"id":"T9","span":{"begin":623,"end":629},"obj":"Gene_or_gene_product"},{"id":"T10","span":{"begin":800,"end":806},"obj":"Gene_or_gene_product"},{"id":"T11","span":{"begin":955,"end":959},"obj":"Gene_or_gene_product"}],"attributes":[{"subj":"T1","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T2","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T3","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T4","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T5","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T6","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T7","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T8","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T9","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T10","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T11","pred":"source","obj":"bionlp-st-pc-2013-training"}]},{"project":"DisGeNET5_gene_disease","denotations":[{"id":"10523842-1#18#34#gene4193","span":{"begin":106,"end":122},"obj":"gene4193"},{"id":"10523842-1#83#101#diseaseC0037579","span":{"begin":171,"end":189},"obj":"diseaseC0037579"}],"relations":[{"id":"18#34#gene419383#101#diseaseC0037579","pred":"associated_with","subj":"10523842-1#18#34#gene4193","obj":"10523842-1#83#101#diseaseC0037579"}],"attributes":[{"subj":"10523842-1#18#34#gene4193","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"10523842-1#83#101#diseaseC0037579","pred":"source","obj":"DisGeNET5_gene_disease"}]},{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":106,"end":122},"obj":"gene:4193"},{"id":"T1","span":{"begin":171,"end":189},"obj":"disease:C0037579"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"attributes":[{"subj":"T0","pred":"source","obj":"DisGeNET"},{"subj":"T1","pred":"source","obj":"DisGeNET"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PubmedHPO","color":"#ec93eb","default":true},{"id":"bionlp-st-pc-2013-training","color":"#93ecd3"},{"id":"DisGeNET5_gene_disease","color":"#ecb993"},{"id":"DisGeNET","color":"#9f93ec"}]}]}}