PubMed:10446065 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10446065","sourcedb":"PubMed","sourceid":"10446065","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10446065","text":"Transcriptional inhibition by interleukin-6 of the class A macrophage scavenger receptor in macrophages derived from human peripheral monocytes and the THP-1 monocytic cell line.\nExpression of the class A macrophage scavenger receptor (MSR) contributes to the uptake of modified low density lipoproteins (LDL) by macrophages and transformation of these cells into lipid-laden foam cells, which characterize atherosclerosis. Many environmental factors, in particular, proinflammatory cytokines and growth factors, can exert regulatory effects on MSR expression, whereas intracellular accumulation of cholesterol itself does not influence MSR levels to any considerable extent. In the present study, by using an in vitro model, we examined whether stimulation with interleukin-6 (IL-6), an immunoregulatory, multipotential cytokine, modulates the expression and activities of the MSR in macrophages. When treated with IL-6, macrophages derived from peripheral monocytes and phorbol 12-myristate 13-acetate (PMA)-differentiated THP-1 monocytic cells showed significantly reduced uptake and/or binding of the MSR ligand, acetylated LDL. This effect was paralleled by a reduction in the expression of MSR protein and mRNA. Analysis of MSR promoter activity in THP-1 cells transfected with an MSR promoter-reporter gene construct demonstrated decreased activity of the MSR promoter in IL-6-treated THP-1 macrophages. Electrophoretic mobility gel shift assay also showed a reduction in the binding of a transcription factor to the MSR promoter AP-1/ets elements in IL-6-treated cells. Thus, exposure to IL-6 may inhibit expression of the class A MSR in differentiated macrophages at transcriptional levels. This result suggests that this cytokine may modulate foam cell formation during atherogenesis.","tracks":[{"project":"genia-medco-coref","denotations":[{"id":"C21","span":{"begin":30,"end":43},"obj":"NP"},{"id":"C2","span":{"begin":47,"end":88},"obj":"NP"},{"id":"C3","span":{"begin":92,"end":177},"obj":"NP"},{"id":"C1","span":{"begin":193,"end":240},"obj":"NP"},{"id":"C23","span":{"begin":270,"end":309},"obj":"NP"},{"id":"C4","span":{"begin":313,"end":324},"obj":"NP"},{"id":"C24","span":{"begin":347,"end":358},"obj":"NP"},{"id":"C25","span":{"begin":364,"end":386},"obj":"NP"},{"id":"C26","span":{"begin":388,"end":393},"obj":"NP"},{"id":"C27","span":{"begin":424,"end":450},"obj":"NP"},{"id":"C28","span":{"begin":467,"end":511},"obj":"NP"},{"id":"C30","span":{"begin":569,"end":610},"obj":"NP"},{"id":"C29","span":{"begin":611,"end":617},"obj":"NP"},{"id":"C31","span":{"begin":763,"end":783},"obj":"NP"},{"id":"C32","span":{"begin":785,"end":829},"obj":"NP"},{"id":"C7","span":{"begin":874,"end":881},"obj":"NP"},{"id":"C8","span":{"begin":885,"end":896},"obj":"NP"},{"id":"C22","span":{"begin":916,"end":920},"obj":"NP"},{"id":"C9","span":{"begin":922,"end":1046},"obj":"NP"},{"id":"C33","span":{"begin":1101,"end":1115},"obj":"NP"},{"id":"C34","span":{"begin":1117,"end":1131},"obj":"NP"},{"id":"C16","span":{"begin":1287,"end":1300},"obj":"NP"},{"id":"C17","span":{"begin":1359,"end":1375},"obj":"NP"},{"id":"C35","span":{"begin":1379,"end":1384},"obj":"NP"},{"id":"C36","span":{"begin":1558,"end":1563},"obj":"NP"},{"id":"C37","span":{"begin":1596,"end":1600},"obj":"NP"},{"id":"C20","span":{"begin":1627,"end":1642},"obj":"NP"},{"id":"C38","span":{"begin":1726,"end":1739},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C1","obj":"C2"},{"id":"R2","pred":"coref-ident","subj":"C24","obj":"C23"},{"id":"R3","pred":"coref-relat","subj":"C26","obj":"C25"},{"id":"R4","pred":"coref-part-whole","subj":"C28","obj":"C27"},{"id":"R5","pred":"coref-pron","subj":"C29","obj":"C30"},{"id":"R6","pred":"coref-ident","subj":"C31","obj":"C21"},{"id":"R7","pred":"coref-appos","subj":"C32","obj":"C31"},{"id":"R8","pred":"coref-ident","subj":"C7","obj":"C1"},{"id":"R9","pred":"coref-ident","subj":"C8","obj":"C4"},{"id":"R10","pred":"coref-ident","subj":"C22","obj":"C31"},{"id":"R11","pred":"coref-ident","subj":"C9","obj":"C3"},{"id":"R12","pred":"coref-appos","subj":"C34","obj":"C33"},{"id":"R13","pred":"coref-ident","subj":"C17","obj":"C16"},{"id":"R14","pred":"coref-ident","subj":"C35","obj":"C22"},{"id":"R15","pred":"coref-ident","subj":"C36","obj":"C35"},{"id":"R16","pred":"coref-ident","subj":"C37","obj":"C36"},{"id":"R17","pred":"coref-ident","subj":"C20","obj":"C1"},{"id":"R18","pred":"coref-ident","subj":"C38","obj":"C37"}],"attributes":[{"subj":"C21","pred":"source","obj":"genia-medco-coref"},{"subj":"C2","pred":"source","obj":"genia-medco-coref"},{"subj":"C3","pred":"source","obj":"genia-medco-coref"},{"subj":"C1","pred":"source","obj":"genia-medco-coref"},{"subj":"C23","pred":"source","obj":"genia-medco-coref"},{"subj":"C4","pred":"source","obj":"genia-medco-coref"},{"subj":"C24","pred":"source","obj":"genia-medco-coref"},{"subj":"C25","pred":"source","obj":"genia-medco-coref"},{"subj":"C26","pred":"source","obj":"genia-medco-coref"},{"subj":"C27","pred":"source","obj":"genia-medco-coref"},{"subj":"C28","pred":"source","obj":"genia-medco-coref"},{"subj":"C30","pred":"source","obj":"genia-medco-coref"},{"subj":"C29","pred":"source","obj":"genia-medco-coref"},{"subj":"C31","pred":"source","obj":"genia-medco-coref"},{"subj":"C32","pred":"source","obj":"genia-medco-coref"},{"subj":"C7","pred":"source","obj":"genia-medco-coref"},{"subj":"C8","pred":"source","obj":"genia-medco-coref"},{"subj":"C22","pred":"source","obj":"genia-medco-coref"},{"subj":"C9","pred":"source","obj":"genia-medco-coref"},{"subj":"C33","pred":"source","obj":"genia-medco-coref"},{"subj":"C34","pred":"source","obj":"genia-medco-coref"},{"subj":"C16","pred":"source","obj":"genia-medco-coref"},{"subj":"C17","pred":"source","obj":"genia-medco-coref"},{"subj":"C35","pred":"source","obj":"genia-medco-coref"},{"subj":"C36","pred":"source","obj":"genia-medco-coref"},{"subj":"C37","pred":"source","obj":"genia-medco-coref"},{"subj":"C20","pred":"source","obj":"genia-medco-coref"},{"subj":"C38","pred":"source","obj":"genia-medco-coref"}]},{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":376,"end":386},"obj":"HP_0003651"},{"id":"T2","span":{"begin":407,"end":422},"obj":"HP_0002621"},{"id":"T3","span":{"begin":1753,"end":1762},"obj":"HP_0003651"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"},{"subj":"T2","pred":"source","obj":"PubmedHPO"},{"subj":"T3","pred":"source","obj":"PubmedHPO"}]},{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":30,"end":43},"obj":"protein"},{"id":"T2","span":{"begin":51,"end":88},"obj":"protein"},{"id":"T3","span":{"begin":92,"end":103},"obj":"cell_type"},{"id":"T4","span":{"begin":117,"end":143},"obj":"cell_type"},{"id":"T5","span":{"begin":152,"end":177},"obj":"cell_line"},{"id":"T6","span":{"begin":197,"end":240},"obj":"protein"},{"id":"T7","span":{"begin":270,"end":309},"obj":"protein"},{"id":"T8","span":{"begin":313,"end":324},"obj":"cell_type"},{"id":"T9","span":{"begin":364,"end":386},"obj":"cell_type"},{"id":"T10","span":{"begin":467,"end":492},"obj":"protein"},{"id":"T11","span":{"begin":497,"end":511},"obj":"protein"},{"id":"T12","span":{"begin":545,"end":548},"obj":"protein"},{"id":"T13","span":{"begin":637,"end":640},"obj":"protein"},{"id":"T14","span":{"begin":763,"end":783},"obj":"protein"},{"id":"T15","span":{"begin":788,"end":804},"obj":"protein"},{"id":"T16","span":{"begin":806,"end":829},"obj":"protein"},{"id":"T17","span":{"begin":878,"end":881},"obj":"protein"},{"id":"T18","span":{"begin":885,"end":896},"obj":"cell_type"},{"id":"T19","span":{"begin":916,"end":920},"obj":"protein"},{"id":"T20","span":{"begin":922,"end":933},"obj":"cell_type"},{"id":"T21","span":{"begin":947,"end":967},"obj":"cell_type"},{"id":"T22","span":{"begin":972,"end":1046},"obj":"cell_line"},{"id":"T23","span":{"begin":1105,"end":1108},"obj":"protein"},{"id":"T24","span":{"begin":1117,"end":1131},"obj":"protein"},{"id":"T25","span":{"begin":1196,"end":1199},"obj":"protein"},{"id":"T26","span":{"begin":1212,"end":1216},"obj":"RNA"},{"id":"T27","span":{"begin":1230,"end":1242},"obj":"DNA"},{"id":"T28","span":{"begin":1255,"end":1266},"obj":"cell_line"},{"id":"T29","span":{"begin":1287,"end":1323},"obj":"DNA"},{"id":"T30","span":{"begin":1363,"end":1375},"obj":"DNA"},{"id":"T31","span":{"begin":1379,"end":1383},"obj":"protein"},{"id":"T32","span":{"begin":1392,"end":1397},"obj":"cell_line"},{"id":"T33","span":{"begin":1398,"end":1409},"obj":"cell_type"},{"id":"T34","span":{"begin":1496,"end":1516},"obj":"protein"},{"id":"T35","span":{"begin":1524,"end":1554},"obj":"DNA"},{"id":"T36","span":{"begin":1558,"end":1562},"obj":"protein"},{"id":"T37","span":{"begin":1596,"end":1600},"obj":"protein"},{"id":"T38","span":{"begin":1631,"end":1642},"obj":"protein"},{"id":"T39","span":{"begin":1661,"end":1672},"obj":"cell_type"},{"id":"T40","span":{"begin":1731,"end":1739},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"jnlpba-st-training"},{"subj":"T2","pred":"source","obj":"jnlpba-st-training"},{"subj":"T3","pred":"source","obj":"jnlpba-st-training"},{"subj":"T4","pred":"source","obj":"jnlpba-st-training"},{"subj":"T5","pred":"source","obj":"jnlpba-st-training"},{"subj":"T6","pred":"source","obj":"jnlpba-st-training"},{"subj":"T7","pred":"source","obj":"jnlpba-st-training"},{"subj":"T8","pred":"source","obj":"jnlpba-st-training"},{"subj":"T9","pred":"source","obj":"jnlpba-st-training"},{"subj":"T10","pred":"source","obj":"jnlpba-st-training"},{"subj":"T11","pred":"source","obj":"jnlpba-st-training"},{"subj":"T12","pred":"source","obj":"jnlpba-st-training"},{"subj":"T13","pred":"source","obj":"jnlpba-st-training"},{"subj":"T14","pred":"source","obj":"jnlpba-st-training"},{"subj":"T15","pred":"source","obj":"jnlpba-st-training"},{"subj":"T16","pred":"source","obj":"jnlpba-st-training"},{"subj":"T17","pred":"source","obj":"jnlpba-st-training"},{"subj":"T18","pred":"source","obj":"jnlpba-st-training"},{"subj":"T19","pred":"source","obj":"jnlpba-st-training"},{"subj":"T20","pred":"source","obj":"jnlpba-st-training"},{"subj":"T21","pred":"source","obj":"jnlpba-st-training"},{"subj":"T22","pred":"source","obj":"jnlpba-st-training"},{"subj":"T23","pred":"source","obj":"jnlpba-st-training"},{"subj":"T24","pred":"source","obj":"jnlpba-st-training"},{"subj":"T25","pred":"source","obj":"jnlpba-st-training"},{"subj":"T26","pred":"source","obj":"jnlpba-st-training"},{"subj":"T27","pred":"source","obj":"jnlpba-st-training"},{"subj":"T28","pred":"source","obj":"jnlpba-st-training"},{"subj":"T29","pred":"source","obj":"jnlpba-st-training"},{"subj":"T30","pred":"source","obj":"jnlpba-st-training"},{"subj":"T31","pred":"source","obj":"jnlpba-st-training"},{"subj":"T32","pred":"source","obj":"jnlpba-st-training"},{"subj":"T33","pred":"source","obj":"jnlpba-st-training"},{"subj":"T34","pred":"source","obj":"jnlpba-st-training"},{"subj":"T35","pred":"source","obj":"jnlpba-st-training"},{"subj":"T36","pred":"source","obj":"jnlpba-st-training"},{"subj":"T37","pred":"source","obj":"jnlpba-st-training"},{"subj":"T38","pred":"source","obj":"jnlpba-st-training"},{"subj":"T39","pred":"source","obj":"jnlpba-st-training"},{"subj":"T40","pred":"source","obj":"jnlpba-st-training"}]},{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":178},"obj":"Sentence"},{"id":"S2","span":{"begin":179,"end":423},"obj":"Sentence"},{"id":"S3","span":{"begin":424,"end":675},"obj":"Sentence"},{"id":"S4","span":{"begin":676,"end":897},"obj":"Sentence"},{"id":"S5","span":{"begin":898,"end":1132},"obj":"Sentence"},{"id":"S6","span":{"begin":1133,"end":1217},"obj":"Sentence"},{"id":"S7","span":{"begin":1218,"end":1410},"obj":"Sentence"},{"id":"S8","span":{"begin":1411,"end":1577},"obj":"Sentence"},{"id":"S9","span":{"begin":1578,"end":1699},"obj":"Sentence"},{"id":"S10","span":{"begin":1700,"end":1794},"obj":"Sentence"}],"attributes":[{"subj":"S1","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S2","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S3","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S4","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S5","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S6","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S7","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S8","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S9","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S10","pred":"source","obj":"pubmed-sentences-benchmark"}]},{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":0,"end":26},"obj":"other_name"},{"id":"T2","span":{"begin":30,"end":43},"obj":"protein_molecule"},{"id":"T3","span":{"begin":92,"end":103},"obj":"cell_type"},{"id":"T4","span":{"begin":117,"end":143},"obj":"cell_type"},{"id":"T5","span":{"begin":152,"end":157},"obj":"cell_line"},{"id":"T6","span":{"begin":197,"end":236},"obj":"protein_complex"},{"id":"T7","span":{"begin":236,"end":239},"obj":"protein_complex"},{"id":"T8","span":{"begin":270,"end":305},"obj":"protein_family_or_group"},{"id":"T9","span":{"begin":305,"end":308},"obj":"protein_family_or_group"},{"id":"T10","span":{"begin":313,"end":324},"obj":"cell_type"},{"id":"T11","span":{"begin":364,"end":386},"obj":"cell_type"},{"id":"T12","span":{"begin":407,"end":422},"obj":"other_name"},{"id":"T13","span":{"begin":467,"end":492},"obj":"protein_family_or_group"},{"id":"T14","span":{"begin":497,"end":511},"obj":"protein_family_or_group"},{"id":"T15","span":{"begin":545,"end":548},"obj":"protein_complex"},{"id":"T16","span":{"begin":637,"end":640},"obj":"protein_complex"},{"id":"T17","span":{"begin":763,"end":783},"obj":"protein_molecule"},{"id":"T18","span":{"begin":788,"end":804},"obj":"protein_family_or_group"},{"id":"T19","span":{"begin":806,"end":829},"obj":"protein_family_or_group"},{"id":"T20","span":{"begin":878,"end":881},"obj":"protein_complex"},{"id":"T21","span":{"begin":885,"end":896},"obj":"cell_type"},{"id":"T22","span":{"begin":916,"end":920},"obj":"protein_molecule"},{"id":"T23","span":{"begin":922,"end":933},"obj":"cell_type"},{"id":"T24","span":{"begin":947,"end":967},"obj":"cell_type"},{"id":"T25","span":{"begin":972,"end":1046},"obj":"cell_line"},{"id":"T26","span":{"begin":1105,"end":1108},"obj":"protein_complex"},{"id":"T27","span":{"begin":1117,"end":1127},"obj":"protein_family_or_group"},{"id":"T28","span":{"begin":1128,"end":1131},"obj":"protein_family_or_group"},{"id":"T29","span":{"begin":1196,"end":1199},"obj":"protein_complex"},{"id":"T30","span":{"begin":1212,"end":1216},"obj":"RNA_family_or_group"},{"id":"T31","span":{"begin":1230,"end":1233},"obj":"protein_complex"},{"id":"T32","span":{"begin":1255,"end":1260},"obj":"cell_line"},{"id":"T33","span":{"begin":1287,"end":1290},"obj":"protein_complex"},{"id":"T34","span":{"begin":1363,"end":1366},"obj":"protein_complex"},{"id":"T35","span":{"begin":1379,"end":1383},"obj":"protein_molecule"},{"id":"T36","span":{"begin":1392,"end":1397},"obj":"cell_line"},{"id":"T37","span":{"begin":1398,"end":1409},"obj":"cell_type"},{"id":"T38","span":{"begin":1411,"end":1451},"obj":"other_name"},{"id":"T39","span":{"begin":1496,"end":1516},"obj":"protein_family_or_group"},{"id":"T40","span":{"begin":1524,"end":1527},"obj":"protein_complex"},{"id":"T41","span":{"begin":1558,"end":1562},"obj":"protein_molecule"},{"id":"T42","span":{"begin":1596,"end":1600},"obj":"protein_molecule"},{"id":"T43","span":{"begin":1631,"end":1638},"obj":"protein_family_or_group"},{"id":"T44","span":{"begin":1639,"end":1642},"obj":"protein_complex"},{"id":"T45","span":{"begin":1661,"end":1672},"obj":"cell_type"},{"id":"T46","span":{"begin":1731,"end":1739},"obj":"protein_family_or_group"},{"id":"T47","span":{"begin":1780,"end":1793},"obj":"other_name"}],"attributes":[{"subj":"T1","pred":"source","obj":"GENIAcorpus"},{"subj":"T2","pred":"source","obj":"GENIAcorpus"},{"subj":"T3","pred":"source","obj":"GENIAcorpus"},{"subj":"T4","pred":"source","obj":"GENIAcorpus"},{"subj":"T5","pred":"source","obj":"GENIAcorpus"},{"subj":"T6","pred":"source","obj":"GENIAcorpus"},{"subj":"T7","pred":"source","obj":"GENIAcorpus"},{"subj":"T8","pred":"source","obj":"GENIAcorpus"},{"subj":"T9","pred":"source","obj":"GENIAcorpus"},{"subj":"T10","pred":"source","obj":"GENIAcorpus"},{"subj":"T11","pred":"source","obj":"GENIAcorpus"},{"subj":"T12","pred":"source","obj":"GENIAcorpus"},{"subj":"T13","pred":"source","obj":"GENIAcorpus"},{"subj":"T14","pred":"source","obj":"GENIAcorpus"},{"subj":"T15","pred":"source","obj":"GENIAcorpus"},{"subj":"T16","pred":"source","obj":"GENIAcorpus"},{"subj":"T17","pred":"source","obj":"GENIAcorpus"},{"subj":"T18","pred":"source","obj":"GENIAcorpus"},{"subj":"T19","pred":"source","obj":"GENIAcorpus"},{"subj":"T20","pred":"source","obj":"GENIAcorpus"},{"subj":"T21","pred":"source","obj":"GENIAcorpus"},{"subj":"T22","pred":"source","obj":"GENIAcorpus"},{"subj":"T23","pred":"source","obj":"GENIAcorpus"},{"subj":"T24","pred":"source","obj":"GENIAcorpus"},{"subj":"T25","pred":"source","obj":"GENIAcorpus"},{"subj":"T26","pred":"source","obj":"GENIAcorpus"},{"subj":"T27","pred":"source","obj":"GENIAcorpus"},{"subj":"T28","pred":"source","obj":"GENIAcorpus"},{"subj":"T29","pred":"source","obj":"GENIAcorpus"},{"subj":"T30","pred":"source","obj":"GENIAcorpus"},{"subj":"T31","pred":"source","obj":"GENIAcorpus"},{"subj":"T32","pred":"source","obj":"GENIAcorpus"},{"subj":"T33","pred":"source","obj":"GENIAcorpus"},{"subj":"T34","pred":"source","obj":"GENIAcorpus"},{"subj":"T35","pred":"source","obj":"GENIAcorpus"},{"subj":"T36","pred":"source","obj":"GENIAcorpus"},{"subj":"T37","pred":"source","obj":"GENIAcorpus"},{"subj":"T38","pred":"source","obj":"GENIAcorpus"},{"subj":"T39","pred":"source","obj":"GENIAcorpus"},{"subj":"T40","pred":"source","obj":"GENIAcorpus"},{"subj":"T41","pred":"source","obj":"GENIAcorpus"},{"subj":"T42","pred":"source","obj":"GENIAcorpus"},{"subj":"T43","pred":"source","obj":"GENIAcorpus"},{"subj":"T44","pred":"source","obj":"GENIAcorpus"},{"subj":"T45","pred":"source","obj":"GENIAcorpus"},{"subj":"T46","pred":"source","obj":"GENIAcorpus"},{"subj":"T47","pred":"source","obj":"GENIAcorpus"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"genia-medco-coref","color":"#93ecc9","default":true},{"id":"PubmedHPO","color":"#ecaf93"},{"id":"jnlpba-st-training","color":"#9493ec"},{"id":"pubmed-sentences-benchmark","color":"#abec93"},{"id":"GENIAcorpus","color":"#ec93c5"}]}]}}