PubMed:10426278 JSONTXT

{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":188,"end":191},"obj":"protein"},{"id":"T2","span":{"begin":227,"end":240},"obj":"protein"},{"id":"T3","span":{"begin":389,"end":402},"obj":"protein"},{"id":"T4","span":{"begin":429,"end":502},"obj":"cell_line"},{"id":"T5","span":{"begin":810,"end":816},"obj":"cell_type"},{"id":"T6","span":{"begin":886,"end":892},"obj":"cell_type"},{"id":"T7","span":{"begin":903,"end":915},"obj":"protein"},{"id":"T8","span":{"begin":931,"end":934},"obj":"cell_type"},{"id":"T9","span":{"begin":954,"end":987},"obj":"cell_line"},{"id":"T10","span":{"begin":991,"end":995},"obj":"cell_type"},{"id":"T11","span":{"begin":1058,"end":1064},"obj":"cell_type"},{"id":"T12","span":{"begin":1155,"end":1167},"obj":"cell_line"},{"id":"T13","span":{"begin":1186,"end":1189},"obj":"protein"}],"text":"MHC-peptide ligand interactions establish a functional threshold for antigen-specific T cell recognition.\nAntigen-specific T cell recognition is dependent on the functional density of the TCR-ligand, which consists of specific MHC molecules and a specifically bound peptide. We have examined the influence of the affinity and concentration of exogenous peptide and the density of specific MHC molecules on the proliferation of a CD4+, DQA1*0501/DQB1*0201 (DQ2.1)-restricted, HSV-2-specific T cell clone. Using antigen peptide analogs with different mutations of known DQ2-anchor residues, T cell response was reduced in an peptide-affinity and - concentration specific manner. The decrease using weaker binding peptides was gradual as stimulation with a peptide with intermediate affinity yielded intermediate T cell proliferation and the poorest binding peptide induced an even weaker T cell response. MHC class II density on the APC was modified using DQ2 homo- and heterozygous B-LCLs as APCs, however this variation of MHC concentration had no effect on T cell proliferation. We interpret this as a reflection of a low threshold for activation of the T cell clone, in which peptide-MHC avidity is the over-riding determinant of the strength of ligand signal."}

{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":0,"end":11},"obj":"peptide"},{"id":"T2","span":{"begin":69,"end":104},"obj":"other_name"},{"id":"T3","span":{"begin":106,"end":141},"obj":"other_name"},{"id":"T4","span":{"begin":188,"end":191},"obj":"protein_family_or_group"},{"id":"T5","span":{"begin":227,"end":240},"obj":"protein_family_or_group"},{"id":"T6","span":{"begin":247,"end":273},"obj":"peptide"},{"id":"T7","span":{"begin":343,"end":360},"obj":"peptide"},{"id":"T8","span":{"begin":389,"end":402},"obj":"protein_family_or_group"},{"id":"T9","span":{"begin":429,"end":474},"obj":"cell_line"},{"id":"T10","span":{"begin":475,"end":480},"obj":"virus"},{"id":"T11","span":{"begin":510,"end":533},"obj":"peptide"},{"id":"T12","span":{"begin":568,"end":587},"obj":"amino_acid_monomer"},{"id":"T13","span":{"begin":589,"end":604},"obj":"other_name"},{"id":"T14","span":{"begin":623,"end":675},"obj":"other_name"},{"id":"T15","span":{"begin":696,"end":719},"obj":"peptide"},{"id":"T16","span":{"begin":754,"end":761},"obj":"peptide"},{"id":"T17","span":{"begin":810,"end":816},"obj":"cell_type"},{"id":"T18","span":{"begin":839,"end":862},"obj":"peptide"},{"id":"T19","span":{"begin":886,"end":892},"obj":"cell_type"},{"id":"T20","span":{"begin":903,"end":915},"obj":"protein_family_or_group"},{"id":"T21","span":{"begin":931,"end":934},"obj":"cell_type"},{"id":"T22","span":{"begin":991,"end":995},"obj":"cell_type"},{"id":"T23","span":{"begin":1058,"end":1064},"obj":"cell_type"},{"id":"T24","span":{"begin":1155,"end":1161},"obj":"cell_type"},{"id":"T25","span":{"begin":1178,"end":1186},"obj":"other_name"},{"id":"T26","span":{"begin":1186,"end":1189},"obj":"protein_complex"},{"id":"T27","span":{"begin":1248,"end":1261},"obj":"other_name"}],"text":"MHC-peptide ligand interactions establish a functional threshold for antigen-specific T cell recognition.\nAntigen-specific T cell recognition is dependent on the functional density of the TCR-ligand, which consists of specific MHC molecules and a specifically bound peptide. We have examined the influence of the affinity and concentration of exogenous peptide and the density of specific MHC molecules on the proliferation of a CD4+, DQA1*0501/DQB1*0201 (DQ2.1)-restricted, HSV-2-specific T cell clone. Using antigen peptide analogs with different mutations of known DQ2-anchor residues, T cell response was reduced in an peptide-affinity and - concentration specific manner. The decrease using weaker binding peptides was gradual as stimulation with a peptide with intermediate affinity yielded intermediate T cell proliferation and the poorest binding peptide induced an even weaker T cell response. MHC class II density on the APC was modified using DQ2 homo- and heterozygous B-LCLs as APCs, however this variation of MHC concentration had no effect on T cell proliferation. We interpret this as a reflection of a low threshold for activation of the T cell clone, in which peptide-MHC avidity is the over-riding determinant of the strength of ligand signal."}

{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":69,"end":104},"obj":"NP"},{"id":"C2","span":{"begin":106,"end":141},"obj":"NP"},{"id":"C3","span":{"begin":184,"end":198},"obj":"NP"},{"id":"C4","span":{"begin":200,"end":205},"obj":"NP"},{"id":"C5","span":{"begin":1151,"end":1167},"obj":"NP"},{"id":"C6","span":{"begin":1172,"end":1177},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C2","obj":"C1"},{"id":"R2","pred":"coref-relat","subj":"C4","obj":"C3"},{"id":"R3","pred":"coref-relat","subj":"C6","obj":"C5"}],"text":"MHC-peptide ligand interactions establish a functional threshold for antigen-specific T cell recognition.\nAntigen-specific T cell recognition is dependent on the functional density of the TCR-ligand, which consists of specific MHC molecules and a specifically bound peptide. We have examined the influence of the affinity and concentration of exogenous peptide and the density of specific MHC molecules on the proliferation of a CD4+, DQA1*0501/DQB1*0201 (DQ2.1)-restricted, HSV-2-specific T cell clone. Using antigen peptide analogs with different mutations of known DQ2-anchor residues, T cell response was reduced in an peptide-affinity and - concentration specific manner. The decrease using weaker binding peptides was gradual as stimulation with a peptide with intermediate affinity yielded intermediate T cell proliferation and the poorest binding peptide induced an even weaker T cell response. MHC class II density on the APC was modified using DQ2 homo- and heterozygous B-LCLs as APCs, however this variation of MHC concentration had no effect on T cell proliferation. We interpret this as a reflection of a low threshold for activation of the T cell clone, in which peptide-MHC avidity is the over-riding determinant of the strength of ligand signal."}

{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":105},"obj":"Sentence"},{"id":"S2","span":{"begin":106,"end":274},"obj":"Sentence"},{"id":"S3","span":{"begin":275,"end":503},"obj":"Sentence"},{"id":"S4","span":{"begin":504,"end":676},"obj":"Sentence"},{"id":"S5","span":{"begin":677,"end":902},"obj":"Sentence"},{"id":"S6","span":{"begin":903,"end":1079},"obj":"Sentence"},{"id":"S7","span":{"begin":1080,"end":1262},"obj":"Sentence"}],"text":"MHC-peptide ligand interactions establish a functional threshold for antigen-specific T cell recognition.\nAntigen-specific T cell recognition is dependent on the functional density of the TCR-ligand, which consists of specific MHC molecules and a specifically bound peptide. We have examined the influence of the affinity and concentration of exogenous peptide and the density of specific MHC molecules on the proliferation of a CD4+, DQA1*0501/DQB1*0201 (DQ2.1)-restricted, HSV-2-specific T cell clone. Using antigen peptide analogs with different mutations of known DQ2-anchor residues, T cell response was reduced in an peptide-affinity and - concentration specific manner. The decrease using weaker binding peptides was gradual as stimulation with a peptide with intermediate affinity yielded intermediate T cell proliferation and the poorest binding peptide induced an even weaker T cell response. MHC class II density on the APC was modified using DQ2 homo- and heterozygous B-LCLs as APCs, however this variation of MHC concentration had no effect on T cell proliferation. We interpret this as a reflection of a low threshold for activation of the T cell clone, in which peptide-MHC avidity is the over-riding determinant of the strength of ligand signal."}