PubMed:10400605 JSONTXT

{"project":"PMID_GLOBAL","denotations":[{"id":"T1","span":{"begin":716,"end":719},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"0004739"}],"text":"Biochemical and genetic evidence for participation of DevR in a phosphorelay signal transduction pathway essential for heterocyst maturation in Nostoc punctiforme ATCC 29133.\nIn a test of the hypothesis that DevR is a response regulator protein that functions in a phosphorelay signal transduction system involved in heterocyst development in Nostoc punctiforme ATCC 29133, purified affinity-tagged DevR was shown to be phosphorylated in vitro by the noncognate sensor kinase EnvZ. Site-directed mutagenesis was used to generate N. punctiforme mutants with single amino acid substitutions at the putative phosphorylation site of DevR. These mutants exhibited a Fox- phenotype like the original devR insertion mutant UCD 311, consistent with a phosphotransferase role for DevR."}

{"project":"CyanoBase","denotations":[{"id":"T1","span":{"begin":54,"end":58},"obj":"protein"},{"id":"T2","span":{"begin":208,"end":212},"obj":"protein"},{"id":"T3","span":{"begin":399,"end":403},"obj":"protein"},{"id":"T4","span":{"begin":629,"end":633},"obj":"protein"},{"id":"T5","span":{"begin":694,"end":698},"obj":"protein"},{"id":"T6","span":{"begin":771,"end":775},"obj":"protein"}],"text":"Biochemical and genetic evidence for participation of DevR in a phosphorelay signal transduction pathway essential for heterocyst maturation in Nostoc punctiforme ATCC 29133.\nIn a test of the hypothesis that DevR is a response regulator protein that functions in a phosphorelay signal transduction system involved in heterocyst development in Nostoc punctiforme ATCC 29133, purified affinity-tagged DevR was shown to be phosphorylated in vitro by the noncognate sensor kinase EnvZ. Site-directed mutagenesis was used to generate N. punctiforme mutants with single amino acid substitutions at the putative phosphorylation site of DevR. These mutants exhibited a Fox- phenotype like the original devR insertion mutant UCD 311, consistent with a phosphotransferase role for DevR."}