PubMed:10369669 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10369669","sourcedb":"PubMed","sourceid":"10369669","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10369669","text":"Glycoprotein reglucosylation and nucleotide sugar utilization in the secretory pathway: identification of a nucleoside diphosphatase in the endoplasmic reticulum.\nUDP is generated in the lumen of the endoplasmic reticulum (ER) as a product of the UDP-glucose-dependent glycoprotein reglucosylation in the calnexin/calreticulin cycle. We describe here the identification, purification and characterization of an ER enzyme that hydrolyzes UDP to UMP. This nucleoside diphosphatase is a ubiquitously expressed, soluble 45 kDa glycoprotein devoid of transmembrane domains and KDEL-related ER localization sequences. It requires divalent cations for activity and hydrolyzes UDP, GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. By eliminating UDP, which is an inhibitory product of the UDP-Glc:glycoprotein glucosyltransferase, it is likely to promote reglucosylation reactions involved in glycoprotein folding and quality control in the ER.","tracks":[{"project":"PMID_GLOBAL","denotations":[{"id":"T1","span":{"begin":0,"end":162},"obj":"Sentence"},{"id":"T2","span":{"begin":163,"end":333},"obj":"Sentence"},{"id":"T3","span":{"begin":334,"end":448},"obj":"Sentence"},{"id":"T4","span":{"begin":449,"end":611},"obj":"Sentence"},{"id":"T5","span":{"begin":612,"end":771},"obj":"Sentence"},{"id":"T6","span":{"begin":772,"end":985},"obj":"Sentence"}],"attributes":[{"subj":"T1","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T2","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T3","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T4","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T5","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T6","pred":"source","obj":"PMID_GLOBAL"}]},{"project":"bionlp-st-pc-2013-training","denotations":[{"id":"T1","span":{"begin":108,"end":132},"obj":"Gene_or_gene_product"},{"id":"T2","span":{"begin":140,"end":161},"obj":"Cellular_component"},{"id":"T3","span":{"begin":163,"end":166},"obj":"Simple_chemical"},{"id":"T4","span":{"begin":187,"end":192},"obj":"Cellular_component"},{"id":"T5","span":{"begin":200,"end":221},"obj":"Cellular_component"},{"id":"T6","span":{"begin":223,"end":225},"obj":"Cellular_component"},{"id":"T7","span":{"begin":247,"end":258},"obj":"Simple_chemical"},{"id":"T8","span":{"begin":305,"end":313},"obj":"Gene_or_gene_product"},{"id":"T9","span":{"begin":314,"end":326},"obj":"Gene_or_gene_product"},{"id":"T10","span":{"begin":411,"end":413},"obj":"Cellular_component"},{"id":"T11","span":{"begin":437,"end":440},"obj":"Simple_chemical"},{"id":"T12","span":{"begin":444,"end":447},"obj":"Simple_chemical"},{"id":"T13","span":{"begin":454,"end":478},"obj":"Gene_or_gene_product"},{"id":"T14","span":{"begin":572,"end":576},"obj":"Gene_or_gene_product"},{"id":"T15","span":{"begin":585,"end":587},"obj":"Cellular_component"},{"id":"T16","span":{"begin":624,"end":640},"obj":"Simple_chemical"},{"id":"T17","span":{"begin":669,"end":672},"obj":"Simple_chemical"},{"id":"T18","span":{"begin":674,"end":677},"obj":"Simple_chemical"},{"id":"T19","span":{"begin":682,"end":685},"obj":"Simple_chemical"},{"id":"T20","span":{"begin":704,"end":718},"obj":"Simple_chemical"},{"id":"T21","span":{"begin":720,"end":725},"obj":"Simple_chemical"},{"id":"T22","span":{"begin":729,"end":742},"obj":"Simple_chemical"},{"id":"T23","span":{"begin":748,"end":770},"obj":"Simple_chemical"},{"id":"T24","span":{"begin":787,"end":790},"obj":"Simple_chemical"},{"id":"T25","span":{"begin":830,"end":837},"obj":"Simple_chemical"},{"id":"T26","span":{"begin":838,"end":870},"obj":"Gene_or_gene_product"},{"id":"T27","span":{"begin":982,"end":984},"obj":"Cellular_component"}],"attributes":[{"subj":"T1","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T2","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T3","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T4","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T5","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T6","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T7","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T8","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T9","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T10","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T11","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T12","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T13","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T14","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T15","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T16","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T17","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T18","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T19","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T20","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T21","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T22","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T23","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T24","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T25","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T26","pred":"source","obj":"bionlp-st-pc-2013-training"},{"subj":"T27","pred":"source","obj":"bionlp-st-pc-2013-training"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PMID_GLOBAL","color":"#ecb293","default":true},{"id":"bionlp-st-pc-2013-training","color":"#9893ec"}]}]}}