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PubMed:10362833 JSONTXT

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Glycan-Motif

Id Subject Object Predicate Lexical cue
T1 743-750 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 743-750 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 743-750 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-145 Sentence denotes Use of inhibitors to characterize intermediates in the processing of N-glycans synthesized by insect cells: a metabolic study with Sf9 cell line.
TextSentencer_T2 146-387 Sentence denotes The most frequent type of N-glycan synthesized by lepidopteran Sf9 cells appears to be fucosylated Man3GlcNAc2,and this has been a limitation for a large scale production and utilization of therapeutic glycoproteins in cultured insect cells.
TextSentencer_T3 388-548 Sentence denotes The current knowledge of the protein glycosylation pathway derived from structural studies on recombinant glyco-proteins expressed by using baculovirus vectors.
TextSentencer_T4 549-703 Sentence denotes In this work we provide more direct evidence for the sequential events occurring in the processing of endogenous N-glycoproteins of noninfected Sf9 cells.
TextSentencer_T5 704-957 Sentence denotes By metabolic labeling with radioactive mannose, we characterized the glycan structures which accumulated in the presence of processing inhibitors (castanospermine and swainsonine) and in the presence of an intracellular trafficking inhibitor (monensin).
TextSentencer_T6 958-1153 Sentence denotes We thus demonstrated that from the glycan precursor Glc3Man9GlcNAc2 to GlcNAcMan5(Fuc)GlcNAc2 intermediate, the processing pathway in Sf9 cells paralleled the one demonstrated in mammalian cells.
TextSentencer_T7 1154-1311 Sentence denotes By using monensin, we demonstrated the formation of Man3(Fuc)GlcNAc2 from GlcNAcMan3(Fuc)GlcNAc2, a reaction which has not been described in mammalian cells.
TextSentencer_T8 1312-1454 Sentence denotes Our results support the idea that the hexosaminidase activity is of physiological relevance to the glycosylation pathway and is Golgi located.
T1 0-145 Sentence denotes Use of inhibitors to characterize intermediates in the processing of N-glycans synthesized by insect cells: a metabolic study with Sf9 cell line.
T2 146-387 Sentence denotes The most frequent type of N-glycan synthesized by lepidopteran Sf9 cells appears to be fucosylated Man3GlcNAc2,and this has been a limitation for a large scale production and utilization of therapeutic glycoproteins in cultured insect cells.
T3 388-548 Sentence denotes The current knowledge of the protein glycosylation pathway derived from structural studies on recombinant glyco-proteins expressed by using baculovirus vectors.
T4 549-703 Sentence denotes In this work we provide more direct evidence for the sequential events occurring in the processing of endogenous N-glycoproteins of noninfected Sf9 cells.
T5 704-957 Sentence denotes By metabolic labeling with radioactive mannose, we characterized the glycan structures which accumulated in the presence of processing inhibitors (castanospermine and swainsonine) and in the presence of an intracellular trafficking inhibitor (monensin).
T6 958-1153 Sentence denotes We thus demonstrated that from the glycan precursor Glc3Man9GlcNAc2 to GlcNAcMan5(Fuc)GlcNAc2 intermediate, the processing pathway in Sf9 cells paralleled the one demonstrated in mammalian cells.
T7 1154-1311 Sentence denotes By using monensin, we demonstrated the formation of Man3(Fuc)GlcNAc2 from GlcNAcMan3(Fuc)GlcNAc2, a reaction which has not been described in mammalian cells.
T8 1312-1454 Sentence denotes Our results support the idea that the hexosaminidase activity is of physiological relevance to the glycosylation pathway and is Golgi located.
T1 0-145 Sentence denotes Use of inhibitors to characterize intermediates in the processing of N-glycans synthesized by insect cells: a metabolic study with Sf9 cell line.
T2 146-387 Sentence denotes The most frequent type of N-glycan synthesized by lepidopteran Sf9 cells appears to be fucosylated Man3GlcNAc2,and this has been a limitation for a large scale production and utilization of therapeutic glycoproteins in cultured insect cells.
T3 388-548 Sentence denotes The current knowledge of the protein glycosylation pathway derived from structural studies on recombinant glyco-proteins expressed by using baculovirus vectors.
T4 549-703 Sentence denotes In this work we provide more direct evidence for the sequential events occurring in the processing of endogenous N-glycoproteins of noninfected Sf9 cells.
T5 704-957 Sentence denotes By metabolic labeling with radioactive mannose, we characterized the glycan structures which accumulated in the presence of processing inhibitors (castanospermine and swainsonine) and in the presence of an intracellular trafficking inhibitor (monensin).
T6 958-1153 Sentence denotes We thus demonstrated that from the glycan precursor Glc3Man9GlcNAc2 to GlcNAcMan5(Fuc)GlcNAc2 intermediate, the processing pathway in Sf9 cells paralleled the one demonstrated in mammalian cells.
T7 1154-1311 Sentence denotes By using monensin, we demonstrated the formation of Man3(Fuc)GlcNAc2 from GlcNAcMan3(Fuc)GlcNAc2, a reaction which has not been described in mammalian cells.
T8 1312-1454 Sentence denotes Our results support the idea that the hexosaminidase activity is of physiological relevance to the glycosylation pathway and is Golgi located.

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 34-47 FMAID:179268 denotes intermediates
_T2 34-47 FMAID:74531 denotes intermediates
_T3 34-50 FMAID:180048 denotes intermediates in
_T4 55-70 FMAID:67601 denotes processing of N
_T5 55-70 FMAID:165414 denotes processing of N
_T6 101-106 FMAID:169002 denotes cells
_T7 101-106 FMAID:68646 denotes cells
_T8 213-218 FMAID:68646 denotes cells
_T9 213-218 FMAID:169002 denotes cells
_T10 348-361 FMAID:62925 denotes glycoproteins
_T11 348-361 FMAID:167256 denotes glycoproteins
_T12 381-386 FMAID:169002 denotes cells
_T13 381-386 FMAID:68646 denotes cells
_T14 417-424 FMAID:67257 denotes protein
_T15 417-424 FMAID:165447 denotes protein
_T16 494-508 FMAID:167256 denotes glyco-proteins
_T17 494-508 FMAID:62925 denotes glyco-proteins
_T18 500-508 FMAID:165447 denotes proteins
_T19 500-508 FMAID:67257 denotes proteins
_T20 664-677 FMAID:167256 denotes glycoproteins
_T21 664-677 FMAID:62925 denotes glycoproteins
_T22 697-702 FMAID:68646 denotes cells
_T23 697-702 FMAID:169002 denotes cells
_T24 743-750 FMAID:196796 denotes mannose
_T25 743-750 FMAID:82801 denotes mannose
_T26 1052-1064 FMAID:179268 denotes intermediate
_T27 1052-1064 FMAID:74531 denotes intermediate
_T28 1096-1101 FMAID:169002 denotes cells
_T29 1096-1101 FMAID:68646 denotes cells
_T30 1147-1152 FMAID:169002 denotes cells
_T31 1147-1152 FMAID:68646 denotes cells
_T32 1305-1310 FMAID:169002 denotes cells
_T33 1305-1310 FMAID:68646 denotes cells

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 101-106 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T2 196-208 http://purl.bioontology.org/ontology/NCBITAXON/7088 denotes lepidopteran
T3 213-218 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T4 381-386 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T5 528-539 http://purl.bioontology.org/ontology/NCBITAXON/558017 denotes baculovirus
T6 613-619 http://purl.bioontology.org/ontology/STY/T051 denotes events
T7 697-702 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T8 1096-1101 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T9 1102-1112 http://purl.bioontology.org/ontology/NCBITAXON/871272 denotes paralleled
T10 1147-1152 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T11 1305-1310 http://purl.bioontology.org/ontology/STY/T025 denotes cells

GO-BP

Id Subject Object Predicate Lexical cue
T1 55-78 http://purl.obolibrary.org/obo/GO_0016256 denotes processing of N-glycans
T2 55-78 http://purl.obolibrary.org/obo/GO_0006491 denotes processing of N-glycans
T3 110-119 http://purl.obolibrary.org/obo/GO_0008152 denotes metabolic
T4 707-716 http://purl.obolibrary.org/obo/GO_0008152 denotes metabolic
T5 233-244 http://purl.obolibrary.org/obo/GO_0036065 denotes fucosylated
T6 417-438 http://purl.obolibrary.org/obo/GO_0006486 denotes protein glycosylation
T7 425-438 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation
T8 1411-1424 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation
T9 1193-1202 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T10 1350-1364 http://purl.obolibrary.org/obo/GO_0004563 denotes hexosaminidase
T11 1350-1373 http://purl.obolibrary.org/obo/GO_0015929 denotes hexosaminidase activity

GO-CC

Id Subject Object Predicate Lexical cue
T1 101-106 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2 213-218 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3 381-386 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4 697-702 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5 1096-1101 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6 1147-1152 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7 135-139 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T8 910-923 http://purl.obolibrary.org/obo/GO_0005622 denotes intracellular
T9 1440-1445 http://purl.obolibrary.org/obo/GO_0005794 denotes Golgi

UBERON-AE

Id Subject Object Predicate Lexical cue
T1 300-305 http://purl.obolibrary.org/obo/UBERON_0002542 denotes scale

GlycoBiology-Motifs

Id Subject Object Predicate Lexical cue
T1 69-78 http://rdf.glycoinfo.org/glycan/G00027MO denotes N-glycans
T2 172-180 http://rdf.glycoinfo.org/glycan/G00027MO denotes N-glycan

GlycoBiology-Epitope

Id Subject Object Predicate Lexical cue
PD-GlycoEpitope-B_T1 717-725 id denotes labeling

GlyTouCan-IUPAC

Id Subject Object Predicate Lexical cue
GlycanIUPAC_T1 1040-1043 "http://rdf.glycoinfo.org/glycan/G49112ZN" denotes Fuc
GlycanIUPAC_T2 1211-1214 "http://rdf.glycoinfo.org/glycan/G49112ZN" denotes Fuc
GlycanIUPAC_T3 1239-1242 "http://rdf.glycoinfo.org/glycan/G49112ZN" denotes Fuc
GlycanIUPAC_T4 1040-1043 "http://rdf.glycoinfo.org/glycan/G50059AJ" denotes Fuc
GlycanIUPAC_T5 1211-1214 "http://rdf.glycoinfo.org/glycan/G50059AJ" denotes Fuc
GlycanIUPAC_T6 1239-1242 "http://rdf.glycoinfo.org/glycan/G50059AJ" denotes Fuc
GlycanIUPAC_T7 1040-1043 "http://rdf.glycoinfo.org/glycan/G76218YK" denotes Fuc
GlycanIUPAC_T8 1211-1214 "http://rdf.glycoinfo.org/glycan/G76218YK" denotes Fuc
GlycanIUPAC_T9 1239-1242 "http://rdf.glycoinfo.org/glycan/G76218YK" denotes Fuc
GlycanIUPAC_T10 1040-1043 "http://rdf.glycoinfo.org/glycan/G50102KR" denotes Fuc
GlycanIUPAC_T11 1211-1214 "http://rdf.glycoinfo.org/glycan/G50102KR" denotes Fuc
GlycanIUPAC_T12 1239-1242 "http://rdf.glycoinfo.org/glycan/G50102KR" denotes Fuc
GlycanIUPAC_T13 1040-1043 "http://rdf.glycoinfo.org/glycan/G10717VS" denotes Fuc
GlycanIUPAC_T14 1211-1214 "http://rdf.glycoinfo.org/glycan/G10717VS" denotes Fuc
GlycanIUPAC_T15 1239-1242 "http://rdf.glycoinfo.org/glycan/G10717VS" denotes Fuc
GlycanIUPAC_T16 1040-1043 "http://rdf.glycoinfo.org/glycan/G60524RK" denotes Fuc
GlycanIUPAC_T17 1211-1214 "http://rdf.glycoinfo.org/glycan/G60524RK" denotes Fuc
GlycanIUPAC_T18 1239-1242 "http://rdf.glycoinfo.org/glycan/G60524RK" denotes Fuc
GlycanIUPAC_T19 1040-1043 "http://rdf.glycoinfo.org/glycan/G84407TT" denotes Fuc
GlycanIUPAC_T20 1211-1214 "http://rdf.glycoinfo.org/glycan/G84407TT" denotes Fuc
GlycanIUPAC_T21 1239-1242 "http://rdf.glycoinfo.org/glycan/G84407TT" denotes Fuc
GlycanIUPAC_T22 1040-1043 "http://rdf.glycoinfo.org/glycan/G64717JT" denotes Fuc
GlycanIUPAC_T23 1211-1214 "http://rdf.glycoinfo.org/glycan/G64717JT" denotes Fuc
GlycanIUPAC_T24 1239-1242 "http://rdf.glycoinfo.org/glycan/G64717JT" denotes Fuc
GlycanIUPAC_T25 1040-1043 "http://rdf.glycoinfo.org/glycan/G45003TT" denotes Fuc
GlycanIUPAC_T26 1211-1214 "http://rdf.glycoinfo.org/glycan/G45003TT" denotes Fuc
GlycanIUPAC_T27 1239-1242 "http://rdf.glycoinfo.org/glycan/G45003TT" denotes Fuc
GlycanIUPAC_T28 1040-1043 "http://rdf.glycoinfo.org/glycan/G73923FP" denotes Fuc
GlycanIUPAC_T29 1211-1214 "http://rdf.glycoinfo.org/glycan/G73923FP" denotes Fuc
GlycanIUPAC_T30 1239-1242 "http://rdf.glycoinfo.org/glycan/G73923FP" denotes Fuc
GlycanIUPAC_T31 1040-1043 "http://rdf.glycoinfo.org/glycan/G78231MB" denotes Fuc
GlycanIUPAC_T32 1211-1214 "http://rdf.glycoinfo.org/glycan/G78231MB" denotes Fuc
GlycanIUPAC_T33 1239-1242 "http://rdf.glycoinfo.org/glycan/G78231MB" denotes Fuc
GlycanIUPAC_T34 1040-1043 "http://rdf.glycoinfo.org/glycan/G80487UG" denotes Fuc
GlycanIUPAC_T35 1211-1214 "http://rdf.glycoinfo.org/glycan/G80487UG" denotes Fuc
GlycanIUPAC_T36 1239-1242 "http://rdf.glycoinfo.org/glycan/G80487UG" denotes Fuc
GlycanIUPAC_T37 1040-1043 "http://rdf.glycoinfo.org/glycan/G29758MI" denotes Fuc
GlycanIUPAC_T38 1211-1214 "http://rdf.glycoinfo.org/glycan/G29758MI" denotes Fuc
GlycanIUPAC_T39 1239-1242 "http://rdf.glycoinfo.org/glycan/G29758MI" denotes Fuc
GlycanIUPAC_T40 1040-1043 "http://rdf.glycoinfo.org/glycan/G02671KD" denotes Fuc
GlycanIUPAC_T41 1211-1214 "http://rdf.glycoinfo.org/glycan/G02671KD" denotes Fuc
GlycanIUPAC_T42 1239-1242 "http://rdf.glycoinfo.org/glycan/G02671KD" denotes Fuc
GlycanIUPAC_T43 1040-1043 "http://rdf.glycoinfo.org/glycan/G57926TZ" denotes Fuc
GlycanIUPAC_T44 1211-1214 "http://rdf.glycoinfo.org/glycan/G57926TZ" denotes Fuc
GlycanIUPAC_T45 1239-1242 "http://rdf.glycoinfo.org/glycan/G57926TZ" denotes Fuc
GlycanIUPAC_T46 1040-1043 "http://rdf.glycoinfo.org/glycan/G41718FD" denotes Fuc
GlycanIUPAC_T47 1211-1214 "http://rdf.glycoinfo.org/glycan/G41718FD" denotes Fuc
GlycanIUPAC_T48 1239-1242 "http://rdf.glycoinfo.org/glycan/G41718FD" denotes Fuc
GlycanIUPAC_T49 1040-1043 "http://rdf.glycoinfo.org/glycan/G99840FL" denotes Fuc
GlycanIUPAC_T50 1211-1214 "http://rdf.glycoinfo.org/glycan/G99840FL" denotes Fuc
GlycanIUPAC_T51 1239-1242 "http://rdf.glycoinfo.org/glycan/G99840FL" denotes Fuc
GlycanIUPAC_T52 1040-1043 "http://rdf.glycoinfo.org/glycan/G64321UX" denotes Fuc
GlycanIUPAC_T53 1211-1214 "http://rdf.glycoinfo.org/glycan/G64321UX" denotes Fuc
GlycanIUPAC_T54 1239-1242 "http://rdf.glycoinfo.org/glycan/G64321UX" denotes Fuc
GlycanIUPAC_T55 1040-1043 "http://rdf.glycoinfo.org/glycan/G11231EG" denotes Fuc
GlycanIUPAC_T56 1211-1214 "http://rdf.glycoinfo.org/glycan/G11231EG" denotes Fuc
GlycanIUPAC_T57 1239-1242 "http://rdf.glycoinfo.org/glycan/G11231EG" denotes Fuc
GlycanIUPAC_T58 1040-1043 "http://rdf.glycoinfo.org/glycan/G62741TN" denotes Fuc
GlycanIUPAC_T59 1211-1214 "http://rdf.glycoinfo.org/glycan/G62741TN" denotes Fuc
GlycanIUPAC_T60 1239-1242 "http://rdf.glycoinfo.org/glycan/G62741TN" denotes Fuc
GlycanIUPAC_T61 1040-1043 "http://rdf.glycoinfo.org/glycan/G51576ZQ" denotes Fuc
GlycanIUPAC_T62 1211-1214 "http://rdf.glycoinfo.org/glycan/G51576ZQ" denotes Fuc
GlycanIUPAC_T63 1239-1242 "http://rdf.glycoinfo.org/glycan/G51576ZQ" denotes Fuc
GlycanIUPAC_T64 1040-1043 "http://rdf.glycoinfo.org/glycan/G66056LD" denotes Fuc
GlycanIUPAC_T65 1211-1214 "http://rdf.glycoinfo.org/glycan/G66056LD" denotes Fuc
GlycanIUPAC_T66 1239-1242 "http://rdf.glycoinfo.org/glycan/G66056LD" denotes Fuc
GlycanIUPAC_T67 1040-1043 "http://rdf.glycoinfo.org/glycan/G02768BF" denotes Fuc
GlycanIUPAC_T68 1211-1214 "http://rdf.glycoinfo.org/glycan/G02768BF" denotes Fuc
GlycanIUPAC_T69 1239-1242 "http://rdf.glycoinfo.org/glycan/G02768BF" denotes Fuc
GlycanIUPAC_T70 1040-1043 "http://rdf.glycoinfo.org/glycan/G26168RO" denotes Fuc
GlycanIUPAC_T71 1211-1214 "http://rdf.glycoinfo.org/glycan/G26168RO" denotes Fuc
GlycanIUPAC_T72 1239-1242 "http://rdf.glycoinfo.org/glycan/G26168RO" denotes Fuc
GlycanIUPAC_T73 1040-1043 "http://rdf.glycoinfo.org/glycan/G42890HL" denotes Fuc
GlycanIUPAC_T74 1211-1214 "http://rdf.glycoinfo.org/glycan/G42890HL" denotes Fuc
GlycanIUPAC_T75 1239-1242 "http://rdf.glycoinfo.org/glycan/G42890HL" denotes Fuc
GlycanIUPAC_T76 1040-1043 "http://rdf.glycoinfo.org/glycan/G17533VU" denotes Fuc
GlycanIUPAC_T77 1211-1214 "http://rdf.glycoinfo.org/glycan/G17533VU" denotes Fuc
GlycanIUPAC_T78 1239-1242 "http://rdf.glycoinfo.org/glycan/G17533VU" denotes Fuc
GlycanIUPAC_T79 1040-1043 "http://rdf.glycoinfo.org/glycan/G34306RG" denotes Fuc
GlycanIUPAC_T80 1211-1214 "http://rdf.glycoinfo.org/glycan/G34306RG" denotes Fuc
GlycanIUPAC_T81 1239-1242 "http://rdf.glycoinfo.org/glycan/G34306RG" denotes Fuc
GlycanIUPAC_T82 1040-1043 "http://rdf.glycoinfo.org/glycan/G88735KU" denotes Fuc
GlycanIUPAC_T83 1211-1214 "http://rdf.glycoinfo.org/glycan/G88735KU" denotes Fuc
GlycanIUPAC_T84 1239-1242 "http://rdf.glycoinfo.org/glycan/G88735KU" denotes Fuc
GlycanIUPAC_T85 1040-1043 "http://rdf.glycoinfo.org/glycan/G82605UA" denotes Fuc
GlycanIUPAC_T86 1211-1214 "http://rdf.glycoinfo.org/glycan/G82605UA" denotes Fuc
GlycanIUPAC_T87 1239-1242 "http://rdf.glycoinfo.org/glycan/G82605UA" denotes Fuc

performance-test

Id Subject Object Predicate Lexical cue
PD-UBERON-AE-B_T1 300-305 http://purl.obolibrary.org/obo/UBERON_0002542 denotes scale

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 1336-1340 OrganismTaxon denotes idea 76236

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 300-305 Body_part denotes scale http://purl.obolibrary.org/obo/UBERON_0002542
T2 910-923 Body_part denotes intracellular http://purl.obolibrary.org/obo/GO_0005622
T3 1440-1445 Body_part denotes Golgi http://purl.obolibrary.org/obo/GO_0005794