PubMed:10358173 JSONTXT

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    jnlpba-st-training

    {"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":0,"end":5},"obj":"protein"},{"id":"T2","span":{"begin":14,"end":45},"obj":"protein"},{"id":"T3","span":{"begin":71,"end":73},"obj":"cell_type"},{"id":"T4","span":{"begin":78,"end":85},"obj":"cell_type"},{"id":"T5","span":{"begin":87,"end":92},"obj":"protein"},{"id":"T6","span":{"begin":98,"end":132},"obj":"protein"},{"id":"T7","span":{"begin":211,"end":221},"obj":"cell_type"},{"id":"T8","span":{"begin":225,"end":234},"obj":"cell_type"},{"id":"T9","span":{"begin":260,"end":265},"obj":"protein"},{"id":"T10","span":{"begin":342,"end":347},"obj":"protein"},{"id":"T11","span":{"begin":405,"end":410},"obj":"protein"},{"id":"T12","span":{"begin":433,"end":438},"obj":"DNA"},{"id":"T13","span":{"begin":444,"end":469},"obj":"DNA"},{"id":"T14","span":{"begin":515,"end":520},"obj":"protein"},{"id":"T15","span":{"begin":530,"end":540},"obj":"protein"},{"id":"T16","span":{"begin":542,"end":551},"obj":"protein"},{"id":"T17","span":{"begin":553,"end":559},"obj":"protein"},{"id":"T18","span":{"begin":565,"end":596},"obj":"protein"},{"id":"T19","span":{"begin":598,"end":603},"obj":"protein"},{"id":"T20","span":{"begin":609,"end":629},"obj":"protein"},{"id":"T21","span":{"begin":643,"end":647},"obj":"protein"},{"id":"T22","span":{"begin":675,"end":678},"obj":"cell_type"},{"id":"T23","span":{"begin":711,"end":716},"obj":"protein"},{"id":"T24","span":{"begin":739,"end":744},"obj":"protein"},{"id":"T25","span":{"begin":767,"end":776},"obj":"protein"},{"id":"T26","span":{"begin":793,"end":800},"obj":"cell_type"},{"id":"T27","span":{"begin":808,"end":816},"obj":"cell_type"},{"id":"T28","span":{"begin":833,"end":838},"obj":"protein"},{"id":"T29","span":{"begin":862,"end":867},"obj":"protein"},{"id":"T30","span":{"begin":923,"end":932},"obj":"protein"},{"id":"T31","span":{"begin":958,"end":962},"obj":"protein"},{"id":"T32","span":{"begin":967,"end":972},"obj":"protein"},{"id":"T33","span":{"begin":997,"end":1002},"obj":"protein"},{"id":"T34","span":{"begin":1012,"end":1021},"obj":"protein"},{"id":"T35","span":{"begin":1026,"end":1031},"obj":"protein"},{"id":"T36","span":{"begin":1062,"end":1067},"obj":"protein"},{"id":"T37","span":{"begin":1089,"end":1094},"obj":"protein"},{"id":"T38","span":{"begin":1132,"end":1137},"obj":"protein"},{"id":"T39","span":{"begin":1159,"end":1164},"obj":"protein"},{"id":"T40","span":{"begin":1197,"end":1221},"obj":"DNA"},{"id":"T41","span":{"begin":1235,"end":1240},"obj":"protein"},{"id":"T42","span":{"begin":1256,"end":1270},"obj":"DNA"},{"id":"T43","span":{"begin":1308,"end":1313},"obj":"protein"},{"id":"T44","span":{"begin":1335,"end":1340},"obj":"protein"},{"id":"T45","span":{"begin":1354,"end":1375},"obj":"cell_line"},{"id":"T46","span":{"begin":1381,"end":1385},"obj":"protein"},{"id":"T47","span":{"begin":1400,"end":1405},"obj":"protein"},{"id":"T48","span":{"begin":1433,"end":1438},"obj":"protein"},{"id":"T49","span":{"begin":1451,"end":1456},"obj":"protein"},{"id":"T50","span":{"begin":1492,"end":1497},"obj":"protein"},{"id":"T51","span":{"begin":1545,"end":1550},"obj":"protein"},{"id":"T52","span":{"begin":1569,"end":1572},"obj":"protein"},{"id":"T53","span":{"begin":1638,"end":1658},"obj":"protein"}],"text":"IL-12 induces IFN regulating factor-1 (IRF-1) gene expression in human NK and T cells.\nIL-12 is a critical immunoregulatory cytokine that promotes cell-mediated immune responses and the differentiation of naive CD4+ cells to Th1 cells; however, relatively few IL-12 target genes have been identified. To better clarify the molecular basis of IL-12 action, we set out to characterize genes up-regulated by IL-12, first by contrasting IL-12- and IFN-alpha-inducible genes. We identified several genes up-regulated by IL-12, namely, MIP-1alpha, MIP-1beta, IL-1RA, and IFN regulatory factor-1 (IRF-1). IRF-1 is a transcription factor regulated by IFNs that is also essential for Th1 responses. We demonstrated that IL-12 directly up-regulates IRF-1 to the same extent as IFN-alpha in normal human T cells and in NK cells. We showed that IL-12 had a direct effect on IRF-1, an effect not mediated indirectly by the induction of IFN-gamma production. Furthermore, IL-2 and IL-12 synergistically induced IRF-1, whereas IFN-alpha and IL-12 did not. The participation of STAT4 in the regulation of IRF-1 was demonstrated in two ways. First, STAT4 was required for the IL-12-dependent transactivation of an IRF-1 reporter construct, and second, STAT4 binding to the IRF-1 promoter was shown using EMSA. In contrast to IL-12, no up-regulation of IRF-1 was found in IL-4-stimulated cells, and IL-4 did not block IL-12-dependent up-regulation of IRF-1. Therefore, IRF-1 may be an important contributor to IL-12 signaling, and we speculate that the defective IL-12 responses seen in IRF-1-/- mice might be attributable, in part, to the absence of this transcription factor."}

    pubmed-sentences-benchmark

    {"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":86},"obj":"Sentence"},{"id":"S2","span":{"begin":87,"end":300},"obj":"Sentence"},{"id":"S3","span":{"begin":301,"end":470},"obj":"Sentence"},{"id":"S4","span":{"begin":471,"end":597},"obj":"Sentence"},{"id":"S5","span":{"begin":598,"end":689},"obj":"Sentence"},{"id":"S6","span":{"begin":690,"end":817},"obj":"Sentence"},{"id":"S7","span":{"begin":818,"end":944},"obj":"Sentence"},{"id":"S8","span":{"begin":945,"end":1040},"obj":"Sentence"},{"id":"S9","span":{"begin":1041,"end":1124},"obj":"Sentence"},{"id":"S10","span":{"begin":1125,"end":1292},"obj":"Sentence"},{"id":"S11","span":{"begin":1293,"end":1439},"obj":"Sentence"},{"id":"S12","span":{"begin":1440,"end":1659},"obj":"Sentence"}],"text":"IL-12 induces IFN regulating factor-1 (IRF-1) gene expression in human NK and T cells.\nIL-12 is a critical immunoregulatory cytokine that promotes cell-mediated immune responses and the differentiation of naive CD4+ cells to Th1 cells; however, relatively few IL-12 target genes have been identified. To better clarify the molecular basis of IL-12 action, we set out to characterize genes up-regulated by IL-12, first by contrasting IL-12- and IFN-alpha-inducible genes. We identified several genes up-regulated by IL-12, namely, MIP-1alpha, MIP-1beta, IL-1RA, and IFN regulatory factor-1 (IRF-1). IRF-1 is a transcription factor regulated by IFNs that is also essential for Th1 responses. We demonstrated that IL-12 directly up-regulates IRF-1 to the same extent as IFN-alpha in normal human T cells and in NK cells. We showed that IL-12 had a direct effect on IRF-1, an effect not mediated indirectly by the induction of IFN-gamma production. Furthermore, IL-2 and IL-12 synergistically induced IRF-1, whereas IFN-alpha and IL-12 did not. The participation of STAT4 in the regulation of IRF-1 was demonstrated in two ways. First, STAT4 was required for the IL-12-dependent transactivation of an IRF-1 reporter construct, and second, STAT4 binding to the IRF-1 promoter was shown using EMSA. In contrast to IL-12, no up-regulation of IRF-1 was found in IL-4-stimulated cells, and IL-4 did not block IL-12-dependent up-regulation of IRF-1. Therefore, IRF-1 may be an important contributor to IL-12 signaling, and we speculate that the defective IL-12 responses seen in IRF-1-/- mice might be attributable, in part, to the absence of this transcription factor."}

    genia-medco-coref

    {"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":0,"end":5},"obj":"NP"},{"id":"C2","span":{"begin":87,"end":92},"obj":"NP"},{"id":"C3","span":{"begin":96,"end":132},"obj":"NP"},{"id":"C4","span":{"begin":133,"end":137},"obj":"NP"},{"id":"C5","span":{"begin":405,"end":410},"obj":"NP"},{"id":"C6","span":{"begin":515,"end":520},"obj":"NP"},{"id":"C7","span":{"begin":565,"end":596},"obj":"NP"},{"id":"C8","span":{"begin":598,"end":603},"obj":"NP"},{"id":"C9","span":{"begin":607,"end":647},"obj":"NP"},{"id":"C10","span":{"begin":648,"end":652},"obj":"NP"},{"id":"C11","span":{"begin":711,"end":716},"obj":"NP"},{"id":"C12","span":{"begin":739,"end":744},"obj":"NP"},{"id":"C13","span":{"begin":833,"end":838},"obj":"NP"},{"id":"C14","span":{"begin":862,"end":867},"obj":"NP"},{"id":"C15","span":{"begin":967,"end":972},"obj":"NP"},{"id":"C16","span":{"begin":997,"end":1002},"obj":"NP"},{"id":"C17","span":{"begin":1026,"end":1031},"obj":"NP"},{"id":"C18","span":{"begin":1062,"end":1067},"obj":"NP"},{"id":"C19","span":{"begin":1089,"end":1094},"obj":"NP"},{"id":"C20","span":{"begin":1132,"end":1137},"obj":"NP"},{"id":"C21","span":{"begin":1308,"end":1313},"obj":"NP"},{"id":"C22","span":{"begin":1335,"end":1340},"obj":"NP"},{"id":"C23","span":{"begin":1433,"end":1438},"obj":"NP"},{"id":"C24","span":{"begin":1451,"end":1456},"obj":"NP"},{"id":"C25","span":{"begin":1633,"end":1658},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C2","obj":"C1"},{"id":"R2","pred":"coref-relat","subj":"C4","obj":"C3"},{"id":"R3","pred":"coref-ident","subj":"C5","obj":"C2"},{"id":"R4","pred":"coref-ident","subj":"C6","obj":"C5"},{"id":"R5","pred":"coref-ident","subj":"C8","obj":"C7"},{"id":"R6","pred":"coref-relat","subj":"C10","obj":"C9"},{"id":"R7","pred":"coref-ident","subj":"C11","obj":"C6"},{"id":"R8","pred":"coref-ident","subj":"C12","obj":"C8"},{"id":"R9","pred":"coref-ident","subj":"C13","obj":"C11"},{"id":"R10","pred":"coref-ident","subj":"C14","obj":"C12"},{"id":"R11","pred":"coref-ident","subj":"C15","obj":"C13"},{"id":"R12","pred":"coref-ident","subj":"C16","obj":"C14"},{"id":"R13","pred":"coref-ident","subj":"C17","obj":"C15"},{"id":"R14","pred":"coref-ident","subj":"C19","obj":"C16"},{"id":"R15","pred":"coref-ident","subj":"C20","obj":"C18"},{"id":"R16","pred":"coref-ident","subj":"C21","obj":"C17"},{"id":"R17","pred":"coref-ident","subj":"C22","obj":"C19"},{"id":"R18","pred":"coref-ident","subj":"C23","obj":"C22"},{"id":"R19","pred":"coref-ident","subj":"C24","obj":"C23"},{"id":"R20","pred":"coref-ident","subj":"C25","obj":"C24"}],"text":"IL-12 induces IFN regulating factor-1 (IRF-1) gene expression in human NK and T cells.\nIL-12 is a critical immunoregulatory cytokine that promotes cell-mediated immune responses and the differentiation of naive CD4+ cells to Th1 cells; however, relatively few IL-12 target genes have been identified. To better clarify the molecular basis of IL-12 action, we set out to characterize genes up-regulated by IL-12, first by contrasting IL-12- and IFN-alpha-inducible genes. We identified several genes up-regulated by IL-12, namely, MIP-1alpha, MIP-1beta, IL-1RA, and IFN regulatory factor-1 (IRF-1). IRF-1 is a transcription factor regulated by IFNs that is also essential for Th1 responses. We demonstrated that IL-12 directly up-regulates IRF-1 to the same extent as IFN-alpha in normal human T cells and in NK cells. We showed that IL-12 had a direct effect on IRF-1, an effect not mediated indirectly by the induction of IFN-gamma production. Furthermore, IL-2 and IL-12 synergistically induced IRF-1, whereas IFN-alpha and IL-12 did not. The participation of STAT4 in the regulation of IRF-1 was demonstrated in two ways. First, STAT4 was required for the IL-12-dependent transactivation of an IRF-1 reporter construct, and second, STAT4 binding to the IRF-1 promoter was shown using EMSA. In contrast to IL-12, no up-regulation of IRF-1 was found in IL-4-stimulated cells, and IL-4 did not block IL-12-dependent up-regulation of IRF-1. Therefore, IRF-1 may be an important contributor to IL-12 signaling, and we speculate that the defective IL-12 responses seen in IRF-1-/- mice might be attributable, in part, to the absence of this transcription factor."}

    GENIAcorpus

    {"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":0,"end":5},"obj":"protein_molecule"},{"id":"T2","span":{"begin":14,"end":39},"obj":"protein_molecule"},{"id":"T3","span":{"begin":39,"end":44},"obj":"protein_molecule"},{"id":"T4","span":{"begin":71,"end":73},"obj":"cell_type"},{"id":"T5","span":{"begin":78,"end":85},"obj":"cell_type"},{"id":"T6","span":{"begin":87,"end":92},"obj":"protein_molecule"},{"id":"T7","span":{"begin":98,"end":132},"obj":"protein_family_or_group"},{"id":"T8","span":{"begin":147,"end":177},"obj":"other_name"},{"id":"T9","span":{"begin":211,"end":221},"obj":"cell_type"},{"id":"T10","span":{"begin":225,"end":234},"obj":"cell_type"},{"id":"T11","span":{"begin":260,"end":265},"obj":"protein_molecule"},{"id":"T12","span":{"begin":342,"end":347},"obj":"protein_molecule"},{"id":"T13","span":{"begin":405,"end":410},"obj":"protein_molecule"},{"id":"T14","span":{"begin":433,"end":438},"obj":"protein_molecule"},{"id":"T15","span":{"begin":444,"end":447},"obj":"protein_family_or_group"},{"id":"T16","span":{"begin":515,"end":520},"obj":"protein_molecule"},{"id":"T17","span":{"begin":530,"end":540},"obj":"protein_molecule"},{"id":"T18","span":{"begin":542,"end":551},"obj":"protein_molecule"},{"id":"T19","span":{"begin":553,"end":559},"obj":"protein_molecule"},{"id":"T20","span":{"begin":565,"end":590},"obj":"protein_molecule"},{"id":"T21","span":{"begin":590,"end":595},"obj":"protein_molecule"},{"id":"T22","span":{"begin":598,"end":603},"obj":"protein_molecule"},{"id":"T23","span":{"begin":609,"end":629},"obj":"protein_family_or_group"},{"id":"T24","span":{"begin":643,"end":647},"obj":"protein_family_or_group"},{"id":"T25","span":{"begin":675,"end":678},"obj":"cell_type"},{"id":"T26","span":{"begin":711,"end":716},"obj":"protein_molecule"},{"id":"T27","span":{"begin":726,"end":738},"obj":"other_name"},{"id":"T28","span":{"begin":739,"end":744},"obj":"protein_molecule"},{"id":"T29","span":{"begin":767,"end":776},"obj":"protein_molecule"},{"id":"T30","span":{"begin":793,"end":800},"obj":"cell_type"},{"id":"T31","span":{"begin":808,"end":816},"obj":"cell_type"},{"id":"T32","span":{"begin":833,"end":838},"obj":"protein_molecule"},{"id":"T33","span":{"begin":862,"end":867},"obj":"protein_molecule"},{"id":"T34","span":{"begin":923,"end":932},"obj":"protein_molecule"},{"id":"T35","span":{"begin":958,"end":962},"obj":"protein_molecule"},{"id":"T36","span":{"begin":967,"end":972},"obj":"protein_molecule"},{"id":"T37","span":{"begin":997,"end":1002},"obj":"protein_molecule"},{"id":"T38","span":{"begin":1012,"end":1021},"obj":"protein_molecule"},{"id":"T39","span":{"begin":1026,"end":1031},"obj":"protein_molecule"},{"id":"T40","span":{"begin":1062,"end":1067},"obj":"protein_molecule"},{"id":"T41","span":{"begin":1089,"end":1094},"obj":"protein_molecule"},{"id":"T42","span":{"begin":1132,"end":1137},"obj":"protein_molecule"},{"id":"T43","span":{"begin":1159,"end":1164},"obj":"protein_molecule"},{"id":"T44","span":{"begin":1197,"end":1202},"obj":"protein_molecule"},{"id":"T45","span":{"begin":1235,"end":1240},"obj":"protein_molecule"},{"id":"T46","span":{"begin":1256,"end":1261},"obj":"protein_molecule"},{"id":"T47","span":{"begin":1287,"end":1291},"obj":"other_name"},{"id":"T48","span":{"begin":1308,"end":1313},"obj":"protein_molecule"},{"id":"T49","span":{"begin":1318,"end":1331},"obj":"other_name"},{"id":"T50","span":{"begin":1335,"end":1340},"obj":"protein_molecule"},{"id":"T51","span":{"begin":1354,"end":1358},"obj":"protein_molecule"},{"id":"T52","span":{"begin":1381,"end":1385},"obj":"protein_molecule"},{"id":"T53","span":{"begin":1400,"end":1405},"obj":"protein_molecule"},{"id":"T54","span":{"begin":1433,"end":1438},"obj":"protein_molecule"},{"id":"T55","span":{"begin":1451,"end":1456},"obj":"protein_molecule"},{"id":"T56","span":{"begin":1492,"end":1497},"obj":"protein_molecule"},{"id":"T57","span":{"begin":1545,"end":1550},"obj":"protein_molecule"},{"id":"T58","span":{"begin":1569,"end":1572},"obj":"protein_family_or_group"},{"id":"T59","span":{"begin":1638,"end":1658},"obj":"protein_family_or_group"}],"text":"IL-12 induces IFN regulating factor-1 (IRF-1) gene expression in human NK and T cells.\nIL-12 is a critical immunoregulatory cytokine that promotes cell-mediated immune responses and the differentiation of naive CD4+ cells to Th1 cells; however, relatively few IL-12 target genes have been identified. To better clarify the molecular basis of IL-12 action, we set out to characterize genes up-regulated by IL-12, first by contrasting IL-12- and IFN-alpha-inducible genes. We identified several genes up-regulated by IL-12, namely, MIP-1alpha, MIP-1beta, IL-1RA, and IFN regulatory factor-1 (IRF-1). IRF-1 is a transcription factor regulated by IFNs that is also essential for Th1 responses. We demonstrated that IL-12 directly up-regulates IRF-1 to the same extent as IFN-alpha in normal human T cells and in NK cells. We showed that IL-12 had a direct effect on IRF-1, an effect not mediated indirectly by the induction of IFN-gamma production. Furthermore, IL-2 and IL-12 synergistically induced IRF-1, whereas IFN-alpha and IL-12 did not. The participation of STAT4 in the regulation of IRF-1 was demonstrated in two ways. First, STAT4 was required for the IL-12-dependent transactivation of an IRF-1 reporter construct, and second, STAT4 binding to the IRF-1 promoter was shown using EMSA. In contrast to IL-12, no up-regulation of IRF-1 was found in IL-4-stimulated cells, and IL-4 did not block IL-12-dependent up-regulation of IRF-1. Therefore, IRF-1 may be an important contributor to IL-12 signaling, and we speculate that the defective IL-12 responses seen in IRF-1-/- mice might be attributable, in part, to the absence of this transcription factor."}