PubMed:10233875
Annnotations
jnlpba-st-training
{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":0,"end":9},"obj":"protein"},{"id":"T2","span":{"begin":37,"end":67},"obj":"DNA"},{"id":"T3","span":{"begin":82,"end":99},"obj":"cell_type"},{"id":"T4","span":{"begin":151,"end":164},"obj":"protein"},{"id":"T5","span":{"begin":166,"end":170},"obj":"protein"},{"id":"T6","span":{"begin":187,"end":221},"obj":"cell_type"},{"id":"T7","span":{"begin":223,"end":227},"obj":"cell_type"},{"id":"T8","span":{"begin":302,"end":306},"obj":"protein"},{"id":"T9","span":{"begin":358,"end":376},"obj":"cell_type"},{"id":"T10","span":{"begin":389,"end":393},"obj":"protein"},{"id":"T11","span":{"begin":439,"end":448},"obj":"RNA"},{"id":"T12","span":{"begin":495,"end":501},"obj":"protein"},{"id":"T13","span":{"begin":542,"end":546},"obj":"protein"},{"id":"T14","span":{"begin":555,"end":562},"obj":"protein"},{"id":"T15","span":{"begin":605,"end":624},"obj":"cell_line"},{"id":"T16","span":{"begin":738,"end":742},"obj":"protein"},{"id":"T17","span":{"begin":780,"end":786},"obj":"protein"},{"id":"T18","span":{"begin":808,"end":812},"obj":"protein"},{"id":"T19","span":{"begin":849,"end":874},"obj":"cell_line"},{"id":"T20","span":{"begin":912,"end":927},"obj":"DNA"},{"id":"T21","span":{"begin":933,"end":945},"obj":"protein"},{"id":"T22","span":{"begin":950,"end":971},"obj":"DNA"},{"id":"T23","span":{"begin":985,"end":998},"obj":"DNA"},{"id":"T24","span":{"begin":1058,"end":1070},"obj":"DNA"},{"id":"T25","span":{"begin":1096,"end":1136},"obj":"DNA"},{"id":"T26","span":{"begin":1226,"end":1237},"obj":"protein"},{"id":"T27","span":{"begin":1261,"end":1273},"obj":"protein"},{"id":"T28","span":{"begin":1318,"end":1322},"obj":"protein"},{"id":"T29","span":{"begin":1395,"end":1409},"obj":"cell_line"},{"id":"T30","span":{"begin":1438,"end":1447},"obj":"protein"},{"id":"T31","span":{"begin":1487,"end":1491},"obj":"protein"}],"text":"NF-kappaB activation is required for C5a-induced interleukin-8 gene expression in mononuclear cells.\nC5a, a potent peptide chemoattractant, stimulates interleukin-8 (IL-8) secretion from peripheral blood mononuclear cells (PBMC). Experiments were conducted to understand the mechanisms for C5a-induced IL-8 production, which was 14-fold greater than that in unstimulated cells by 2 hours. IL-8 secretion was accompanied by accumulation of IL-8 mRNA in the cytosol and by nuclear expression of a kappaB DNA binding activity within 30 minutes. AP-1 but not NF-IL-6 DNA binding activity was also detected in C5a-stimulated PBMC; however, its delayed expression (maximal at 4 hours) suggested a less important role in the rapid production of IL-8. The correlation between C5a-induced kappaB binding activity and IL-8 gene expression was examined in the RAW264.7 macrophage cells using reporter genes directed by the kappaB sequence from IkappaBalpha and IL-8 promoter regions. C5a-induced reporter gene expression was abolished by introducing mutations into the kappaB sites and by coexpression of a dominant negative IkappaBalpha construct resistant to agonist-induced phosphorylation. Pertussis toxin, which ADP-ribosylates the Gi proteins known to couple to the C5a receptor, produced minimal inhibition of C5a-induced IL-8 expression and had little effect on C5a-induced calcium mobilization in RAW264.7 cells. These results suggest that NF-kappaB activation is required for C5a-induced IL-8 gene expression and that this response is mediated primarily through a pertussis toxin-insensitive pathway."}
pubmed-sentences-benchmark
{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":100},"obj":"Sentence"},{"id":"S2","span":{"begin":101,"end":229},"obj":"Sentence"},{"id":"S3","span":{"begin":230,"end":388},"obj":"Sentence"},{"id":"S4","span":{"begin":389,"end":541},"obj":"Sentence"},{"id":"S5","span":{"begin":542,"end":743},"obj":"Sentence"},{"id":"S6","span":{"begin":744,"end":972},"obj":"Sentence"},{"id":"S7","span":{"begin":973,"end":1182},"obj":"Sentence"},{"id":"S8","span":{"begin":1183,"end":1410},"obj":"Sentence"},{"id":"S9","span":{"begin":1411,"end":1599},"obj":"Sentence"}],"text":"NF-kappaB activation is required for C5a-induced interleukin-8 gene expression in mononuclear cells.\nC5a, a potent peptide chemoattractant, stimulates interleukin-8 (IL-8) secretion from peripheral blood mononuclear cells (PBMC). Experiments were conducted to understand the mechanisms for C5a-induced IL-8 production, which was 14-fold greater than that in unstimulated cells by 2 hours. IL-8 secretion was accompanied by accumulation of IL-8 mRNA in the cytosol and by nuclear expression of a kappaB DNA binding activity within 30 minutes. AP-1 but not NF-IL-6 DNA binding activity was also detected in C5a-stimulated PBMC; however, its delayed expression (maximal at 4 hours) suggested a less important role in the rapid production of IL-8. The correlation between C5a-induced kappaB binding activity and IL-8 gene expression was examined in the RAW264.7 macrophage cells using reporter genes directed by the kappaB sequence from IkappaBalpha and IL-8 promoter regions. C5a-induced reporter gene expression was abolished by introducing mutations into the kappaB sites and by coexpression of a dominant negative IkappaBalpha construct resistant to agonist-induced phosphorylation. Pertussis toxin, which ADP-ribosylates the Gi proteins known to couple to the C5a receptor, produced minimal inhibition of C5a-induced IL-8 expression and had little effect on C5a-induced calcium mobilization in RAW264.7 cells. These results suggest that NF-kappaB activation is required for C5a-induced IL-8 gene expression and that this response is mediated primarily through a pertussis toxin-insensitive pathway."}
genia-medco-coref
{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":0,"end":20},"obj":"NP"},{"id":"C2","span":{"begin":101,"end":104},"obj":"NP"},{"id":"C3","span":{"begin":106,"end":138},"obj":"NP"},{"id":"C4","span":{"begin":151,"end":181},"obj":"NP"},{"id":"C5","span":{"begin":290,"end":317},"obj":"NP"},{"id":"C6","span":{"begin":319,"end":324},"obj":"NP"},{"id":"C7","span":{"begin":389,"end":403},"obj":"NP"},{"id":"C8","span":{"begin":542,"end":583},"obj":"NP"},{"id":"C9","span":{"begin":635,"end":638},"obj":"NP"},{"id":"C10","span":{"begin":845,"end":874},"obj":"NP"},{"id":"C11","span":{"begin":1183,"end":1198},"obj":"NP"},{"id":"C12","span":{"begin":1200,"end":1205},"obj":"NP"},{"id":"C13","span":{"begin":1395,"end":1409},"obj":"NP"},{"id":"C14","span":{"begin":1438,"end":1458},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-appos","subj":"C3","obj":"C2"},{"id":"R2","pred":"coref-relat","subj":"C6","obj":"C5"},{"id":"R3","pred":"coref-ident","subj":"C7","obj":"C4"},{"id":"R4","pred":"coref-pron","subj":"C9","obj":"C8"},{"id":"R5","pred":"coref-relat","subj":"C12","obj":"C11"},{"id":"R6","pred":"coref-ident","subj":"C13","obj":"C10"},{"id":"R7","pred":"coref-ident","subj":"C14","obj":"C1"}],"text":"NF-kappaB activation is required for C5a-induced interleukin-8 gene expression in mononuclear cells.\nC5a, a potent peptide chemoattractant, stimulates interleukin-8 (IL-8) secretion from peripheral blood mononuclear cells (PBMC). Experiments were conducted to understand the mechanisms for C5a-induced IL-8 production, which was 14-fold greater than that in unstimulated cells by 2 hours. IL-8 secretion was accompanied by accumulation of IL-8 mRNA in the cytosol and by nuclear expression of a kappaB DNA binding activity within 30 minutes. AP-1 but not NF-IL-6 DNA binding activity was also detected in C5a-stimulated PBMC; however, its delayed expression (maximal at 4 hours) suggested a less important role in the rapid production of IL-8. The correlation between C5a-induced kappaB binding activity and IL-8 gene expression was examined in the RAW264.7 macrophage cells using reporter genes directed by the kappaB sequence from IkappaBalpha and IL-8 promoter regions. C5a-induced reporter gene expression was abolished by introducing mutations into the kappaB sites and by coexpression of a dominant negative IkappaBalpha construct resistant to agonist-induced phosphorylation. Pertussis toxin, which ADP-ribosylates the Gi proteins known to couple to the C5a receptor, produced minimal inhibition of C5a-induced IL-8 expression and had little effect on C5a-induced calcium mobilization in RAW264.7 cells. These results suggest that NF-kappaB activation is required for C5a-induced IL-8 gene expression and that this response is mediated primarily through a pertussis toxin-insensitive pathway."}
GENIAcorpus
{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":0,"end":9},"obj":"protein_complex"},{"id":"T2","span":{"begin":37,"end":40},"obj":"peptide"},{"id":"T3","span":{"begin":82,"end":99},"obj":"cell_type"},{"id":"T4","span":{"begin":101,"end":104},"obj":"peptide"},{"id":"T5","span":{"begin":108,"end":122},"obj":"peptide"},{"id":"T6","span":{"begin":151,"end":164},"obj":"protein_molecule"},{"id":"T7","span":{"begin":166,"end":170},"obj":"protein_molecule"},{"id":"T8","span":{"begin":187,"end":203},"obj":"cell_type"},{"id":"T9","span":{"begin":204,"end":221},"obj":"cell_type"},{"id":"T10","span":{"begin":223,"end":227},"obj":"cell_type"},{"id":"T11","span":{"begin":290,"end":293},"obj":"peptide"},{"id":"T12","span":{"begin":302,"end":306},"obj":"protein_molecule"},{"id":"T13","span":{"begin":358,"end":376},"obj":"cell_type"},{"id":"T14","span":{"begin":389,"end":393},"obj":"protein_molecule"},{"id":"T15","span":{"begin":439,"end":443},"obj":"protein_molecule"},{"id":"T16","span":{"begin":471,"end":489},"obj":"other_name"},{"id":"T17","span":{"begin":495,"end":501},"obj":"protein_molecule"},{"id":"T18","span":{"begin":542,"end":546},"obj":"protein_molecule"},{"id":"T19","span":{"begin":555,"end":562},"obj":"protein_molecule"},{"id":"T20","span":{"begin":605,"end":608},"obj":"peptide"},{"id":"T21","span":{"begin":620,"end":624},"obj":"cell_type"},{"id":"T22","span":{"begin":738,"end":742},"obj":"protein_molecule"},{"id":"T23","span":{"begin":768,"end":771},"obj":"peptide"},{"id":"T24","span":{"begin":780,"end":786},"obj":"protein_molecule"},{"id":"T25","span":{"begin":808,"end":812},"obj":"protein_molecule"},{"id":"T26","span":{"begin":849,"end":874},"obj":"cell_line"},{"id":"T27","span":{"begin":912,"end":927},"obj":"DNA_domain_or_region"},{"id":"T28","span":{"begin":933,"end":945},"obj":"protein_molecule"},{"id":"T29","span":{"begin":950,"end":954},"obj":"protein_molecule"},{"id":"T30","span":{"begin":973,"end":976},"obj":"peptide"},{"id":"T31","span":{"begin":985,"end":998},"obj":"DNA_family_or_group"},{"id":"T32","span":{"begin":1058,"end":1070},"obj":"DNA_domain_or_region"},{"id":"T33","span":{"begin":1096,"end":1113},"obj":"DNA_molecule"},{"id":"T34","span":{"begin":1114,"end":1126},"obj":"protein_molecule"},{"id":"T35","span":{"begin":1150,"end":1181},"obj":"other_name"},{"id":"T36","span":{"begin":1183,"end":1198},"obj":"other_organic_compound"},{"id":"T37","span":{"begin":1206,"end":1209},"obj":"nucleotide"},{"id":"T38","span":{"begin":1226,"end":1237},"obj":"protein_family_or_group"},{"id":"T39","span":{"begin":1261,"end":1264},"obj":"peptide"},{"id":"T40","span":{"begin":1306,"end":1309},"obj":"peptide"},{"id":"T41","span":{"begin":1318,"end":1322},"obj":"protein_molecule"},{"id":"T42","span":{"begin":1359,"end":1362},"obj":"peptide"},{"id":"T43","span":{"begin":1371,"end":1378},"obj":"atom"},{"id":"T44","span":{"begin":1395,"end":1409},"obj":"cell_line"},{"id":"T45","span":{"begin":1438,"end":1447},"obj":"protein_complex"},{"id":"T46","span":{"begin":1475,"end":1478},"obj":"peptide"},{"id":"T47","span":{"begin":1487,"end":1491},"obj":"protein_molecule"},{"id":"T48","span":{"begin":1563,"end":1578},"obj":"other_organic_compound"}],"text":"NF-kappaB activation is required for C5a-induced interleukin-8 gene expression in mononuclear cells.\nC5a, a potent peptide chemoattractant, stimulates interleukin-8 (IL-8) secretion from peripheral blood mononuclear cells (PBMC). Experiments were conducted to understand the mechanisms for C5a-induced IL-8 production, which was 14-fold greater than that in unstimulated cells by 2 hours. IL-8 secretion was accompanied by accumulation of IL-8 mRNA in the cytosol and by nuclear expression of a kappaB DNA binding activity within 30 minutes. AP-1 but not NF-IL-6 DNA binding activity was also detected in C5a-stimulated PBMC; however, its delayed expression (maximal at 4 hours) suggested a less important role in the rapid production of IL-8. The correlation between C5a-induced kappaB binding activity and IL-8 gene expression was examined in the RAW264.7 macrophage cells using reporter genes directed by the kappaB sequence from IkappaBalpha and IL-8 promoter regions. C5a-induced reporter gene expression was abolished by introducing mutations into the kappaB sites and by coexpression of a dominant negative IkappaBalpha construct resistant to agonist-induced phosphorylation. Pertussis toxin, which ADP-ribosylates the Gi proteins known to couple to the C5a receptor, produced minimal inhibition of C5a-induced IL-8 expression and had little effect on C5a-induced calcium mobilization in RAW264.7 cells. These results suggest that NF-kappaB activation is required for C5a-induced IL-8 gene expression and that this response is mediated primarily through a pertussis toxin-insensitive pathway."}