PubMed:10229275 JSONTXT

{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":64,"end":92},"obj":"DNA"},{"id":"T2","span":{"begin":96,"end":124},"obj":"cell_type"},{"id":"T3","span":{"begin":192,"end":214},"obj":"protein"},{"id":"T4","span":{"begin":324,"end":352},"obj":"cell_type"},{"id":"T5","span":{"begin":375,"end":394},"obj":"DNA"},{"id":"T6","span":{"begin":406,"end":460},"obj":"DNA"},{"id":"T7","span":{"begin":486,"end":527},"obj":"DNA"},{"id":"T8","span":{"begin":551,"end":571},"obj":"protein"},{"id":"T9","span":{"begin":572,"end":581},"obj":"protein"},{"id":"T10","span":{"begin":681,"end":698},"obj":"DNA"},{"id":"T11","span":{"begin":732,"end":741},"obj":"protein"},{"id":"T12","span":{"begin":761,"end":770},"obj":"protein"},{"id":"T13","span":{"begin":815,"end":829},"obj":"protein"},{"id":"T14","span":{"begin":859,"end":887},"obj":"DNA"},{"id":"T15","span":{"begin":994,"end":1022},"obj":"cell_type"}],"text":"High molecular weight dextran sulfate increases the activity of NF-kappaB-regulated promoter in monocyte-derived macrophages.\nIt is known that sulfated polysaccharides can mimic the action of common T-cell mitogens. To investigate the molecular basis of the mitogenic effect of high molecular weight dextran sulfate (HMDS), monocyte-derived macrophages were transfected with recombinant plasmid containing chloramphenicol acetyl transferase (CAT) reporter gene under the control of the HIV-1 long terminal repeat (LTR) promoter, which is regulated by transcription factor NF-kappaB. We observed that HMDS, similar to bacterial lipopolysaccharide (LPS), increases the expression of CAT reporter gene suggesting increased activity of NF-kappaB. The activation of NF-kappaB correlated with the increased expression of B7.1 molecules. It was postulated that this NF-kappaB-regulated promoter might play a role in the activation of the accessory cells as well as the rate of replication of HIV-1 in monocyte-derived macrophages."}

{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":125},"obj":"Sentence"},{"id":"S2","span":{"begin":126,"end":215},"obj":"Sentence"},{"id":"S3","span":{"begin":216,"end":582},"obj":"Sentence"},{"id":"S4","span":{"begin":583,"end":742},"obj":"Sentence"},{"id":"S5","span":{"begin":743,"end":830},"obj":"Sentence"},{"id":"S6","span":{"begin":831,"end":1023},"obj":"Sentence"}],"text":"High molecular weight dextran sulfate increases the activity of NF-kappaB-regulated promoter in monocyte-derived macrophages.\nIt is known that sulfated polysaccharides can mimic the action of common T-cell mitogens. To investigate the molecular basis of the mitogenic effect of high molecular weight dextran sulfate (HMDS), monocyte-derived macrophages were transfected with recombinant plasmid containing chloramphenicol acetyl transferase (CAT) reporter gene under the control of the HIV-1 long terminal repeat (LTR) promoter, which is regulated by transcription factor NF-kappaB. We observed that HMDS, similar to bacterial lipopolysaccharide (LPS), increases the expression of CAT reporter gene suggesting increased activity of NF-kappaB. The activation of NF-kappaB correlated with the increased expression of B7.1 molecules. It was postulated that this NF-kappaB-regulated promoter might play a role in the activation of the accessory cells as well as the rate of replication of HIV-1 in monocyte-derived macrophages."}

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