PubMed:10209866 JSONTXT

{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":241,"end":248},"obj":"Glycan_Motif"},{"id":"T2","span":{"begin":316,"end":323},"obj":"Glycan_Motif"},{"id":"T3","span":{"begin":546,"end":553},"obj":"Glycan_Motif"},{"id":"T4","span":{"begin":557,"end":564},"obj":"Glycan_Motif"},{"id":"T5","span":{"begin":568,"end":577},"obj":"Glycan_Motif"},{"id":"T7","span":{"begin":1032,"end":1039},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG"},{"id":"A2","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG"},{"id":"A3","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG"},{"id":"A4","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ"},{"id":"A5","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G68158BT"},{"id":"A6","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G65889KE"},{"id":"A7","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG"}],"text":"Transglycosylation reactions of Bacillus stearothermophilus maltogenic amylase with acarbose and various acceptors.\nIt was observed that Bacillus stearothermophilus maltogenic amylase cleaved the first glycosidic bond of acarbose to produce glucose and a pseudotrisaccharide (PTS) that was transferred to C-6 of the glucose to give an alpha-(1--\u003e6) glycosidic linkage and the formation of isoacarbose. The addition of a number of different carbohydrates to the digest gave transfer products in which PTS was primarily attached alpha-(1--\u003e6) to D-glucose, D-mannose, D-galactose, and methyl alpha-D-glucopyranoside. With D-fructopyranose and D-xylopyranose, PTS was linked alpha-(1--\u003e5) and alpha-(1--\u003e4), respectively. PTS was primarily transferred to C-6 of the nonreducing residue of maltose, cellobiose, lactose, and gentiobiose. Lesser amounts of alpha-(1--\u003e3) and/or alpha-(1--\u003e4) transfer products were also observed for these carbohydrate acceptors. The major transfer product to sucrose gave PTS linked alpha-(1--\u003e4) to the glucose residue. alpha,alpha-Trehalose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4). Maltitol gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the glucopyranose residue. Raffinose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the D-galactopyranose residue. Maltotriose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the nonreducing end glucopyranose residue. Xylitol gave PTS linked alpha-(1--\u003e5) as the major product and D-glucitol gave PTS linked alpha-(1--\u003e6) as the only product. The structures of the transfer products were determined using thin-layer chromatography, high-performance ion chromatography, enzyme hydrolysis, methylation analysis and 13C NMR spectroscopy. The best acceptor was gentiobiose, followed closely by maltose and cellobiose, and the weakest acceptor was D-glucitol."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":115},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":116,"end":401},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":402,"end":614},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":615,"end":718},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":719,"end":832},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":833,"end":956},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":957,"end":1143},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1144,"end":1254},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1255,"end":1370},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":1371,"end":1500},"obj":"Sentence"},{"id":"TextSentencer_T11","span":{"begin":1501,"end":1625},"obj":"Sentence"},{"id":"TextSentencer_T12","span":{"begin":1626,"end":1817},"obj":"Sentence"},{"id":"TextSentencer_T13","span":{"begin":1818,"end":1937},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":115},"obj":"Sentence"},{"id":"T2","span":{"begin":116,"end":401},"obj":"Sentence"},{"id":"T3","span":{"begin":402,"end":614},"obj":"Sentence"},{"id":"T4","span":{"begin":615,"end":718},"obj":"Sentence"},{"id":"T5","span":{"begin":719,"end":832},"obj":"Sentence"},{"id":"T6","span":{"begin":833,"end":956},"obj":"Sentence"},{"id":"T7","span":{"begin":957,"end":1143},"obj":"Sentence"},{"id":"T8","span":{"begin":1144,"end":1254},"obj":"Sentence"},{"id":"T9","span":{"begin":1255,"end":1370},"obj":"Sentence"},{"id":"T10","span":{"begin":1371,"end":1500},"obj":"Sentence"},{"id":"T11","span":{"begin":1501,"end":1625},"obj":"Sentence"},{"id":"T12","span":{"begin":1626,"end":1817},"obj":"Sentence"},{"id":"T13","span":{"begin":1818,"end":1937},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Transglycosylation reactions of Bacillus stearothermophilus maltogenic amylase with acarbose and various acceptors.\nIt was observed that Bacillus stearothermophilus maltogenic amylase cleaved the first glycosidic bond of acarbose to produce glucose and a pseudotrisaccharide (PTS) that was transferred to C-6 of the glucose to give an alpha-(1--\u003e6) glycosidic linkage and the formation of isoacarbose. The addition of a number of different carbohydrates to the digest gave transfer products in which PTS was primarily attached alpha-(1--\u003e6) to D-glucose, D-mannose, D-galactose, and methyl alpha-D-glucopyranoside. With D-fructopyranose and D-xylopyranose, PTS was linked alpha-(1--\u003e5) and alpha-(1--\u003e4), respectively. PTS was primarily transferred to C-6 of the nonreducing residue of maltose, cellobiose, lactose, and gentiobiose. Lesser amounts of alpha-(1--\u003e3) and/or alpha-(1--\u003e4) transfer products were also observed for these carbohydrate acceptors. The major transfer product to sucrose gave PTS linked alpha-(1--\u003e4) to the glucose residue. alpha,alpha-Trehalose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4). Maltitol gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the glucopyranose residue. Raffinose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the D-galactopyranose residue. Maltotriose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the nonreducing end glucopyranose residue. Xylitol gave PTS linked alpha-(1--\u003e5) as the major product and D-glucitol gave PTS linked alpha-(1--\u003e6) as the only product. The structures of the transfer products were determined using thin-layer chromatography, high-performance ion chromatography, enzyme hydrolysis, methylation analysis and 13C NMR spectroscopy. The best acceptor was gentiobiose, followed closely by maltose and cellobiose, and the weakest acceptor was D-glucitol."}

{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":241,"end":248},"obj":"https://glytoucan.org/Structures/Glycans/G15021LG"},{"id":"T2","span":{"begin":316,"end":323},"obj":"https://glytoucan.org/Structures/Glycans/G15021LG"},{"id":"T3","span":{"begin":546,"end":553},"obj":"https://glytoucan.org/Structures/Glycans/G15021LG"},{"id":"T4","span":{"begin":557,"end":564},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"},{"id":"T5","span":{"begin":568,"end":577},"obj":"https://glytoucan.org/Structures/Glycans/G65889KE"},{"id":"T6","span":{"begin":568,"end":577},"obj":"https://glytoucan.org/Structures/Glycans/G68158BT"},{"id":"T7","span":{"begin":1032,"end":1039},"obj":"https://glytoucan.org/Structures/Glycans/G15021LG"}],"text":"Transglycosylation reactions of Bacillus stearothermophilus maltogenic amylase with acarbose and various acceptors.\nIt was observed that Bacillus stearothermophilus maltogenic amylase cleaved the first glycosidic bond of acarbose to produce glucose and a pseudotrisaccharide (PTS) that was transferred to C-6 of the glucose to give an alpha-(1--\u003e6) glycosidic linkage and the formation of isoacarbose. The addition of a number of different carbohydrates to the digest gave transfer products in which PTS was primarily attached alpha-(1--\u003e6) to D-glucose, D-mannose, D-galactose, and methyl alpha-D-glucopyranoside. With D-fructopyranose and D-xylopyranose, PTS was linked alpha-(1--\u003e5) and alpha-(1--\u003e4), respectively. PTS was primarily transferred to C-6 of the nonreducing residue of maltose, cellobiose, lactose, and gentiobiose. Lesser amounts of alpha-(1--\u003e3) and/or alpha-(1--\u003e4) transfer products were also observed for these carbohydrate acceptors. The major transfer product to sucrose gave PTS linked alpha-(1--\u003e4) to the glucose residue. alpha,alpha-Trehalose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4). Maltitol gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the glucopyranose residue. Raffinose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the D-galactopyranose residue. Maltotriose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the nonreducing end glucopyranose residue. Xylitol gave PTS linked alpha-(1--\u003e5) as the major product and D-glucitol gave PTS linked alpha-(1--\u003e6) as the only product. The structures of the transfer products were determined using thin-layer chromatography, high-performance ion chromatography, enzyme hydrolysis, methylation analysis and 13C NMR spectroscopy. The best acceptor was gentiobiose, followed closely by maltose and cellobiose, and the weakest acceptor was D-glucitol."}

{"project":"bionlp-st-epi-2011-training","denotations":[{"id":"T1","span":{"begin":60,"end":78},"obj":"Protein"},{"id":"T2","span":{"begin":165,"end":183},"obj":"Protein"}],"text":"Transglycosylation reactions of Bacillus stearothermophilus maltogenic amylase with acarbose and various acceptors.\nIt was observed that Bacillus stearothermophilus maltogenic amylase cleaved the first glycosidic bond of acarbose to produce glucose and a pseudotrisaccharide (PTS) that was transferred to C-6 of the glucose to give an alpha-(1--\u003e6) glycosidic linkage and the formation of isoacarbose. The addition of a number of different carbohydrates to the digest gave transfer products in which PTS was primarily attached alpha-(1--\u003e6) to D-glucose, D-mannose, D-galactose, and methyl alpha-D-glucopyranoside. With D-fructopyranose and D-xylopyranose, PTS was linked alpha-(1--\u003e5) and alpha-(1--\u003e4), respectively. PTS was primarily transferred to C-6 of the nonreducing residue of maltose, cellobiose, lactose, and gentiobiose. Lesser amounts of alpha-(1--\u003e3) and/or alpha-(1--\u003e4) transfer products were also observed for these carbohydrate acceptors. The major transfer product to sucrose gave PTS linked alpha-(1--\u003e4) to the glucose residue. alpha,alpha-Trehalose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4). Maltitol gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the glucopyranose residue. Raffinose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the D-galactopyranose residue. Maltotriose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the nonreducing end glucopyranose residue. Xylitol gave PTS linked alpha-(1--\u003e5) as the major product and D-glucitol gave PTS linked alpha-(1--\u003e6) as the only product. The structures of the transfer products were determined using thin-layer chromatography, high-performance ion chromatography, enzyme hydrolysis, methylation analysis and 13C NMR spectroscopy. The best acceptor was gentiobiose, followed closely by maltose and cellobiose, and the weakest acceptor was D-glucitol."}

{"project":"Glycan-GlyCosmos","denotations":[{"id":"T1","span":{"begin":786,"end":793},"obj":"Glycan"},{"id":"T2","span":{"begin":795,"end":805},"obj":"Glycan"},{"id":"T3","span":{"begin":807,"end":814},"obj":"Glycan"},{"id":"T4","span":{"begin":820,"end":831},"obj":"Glycan"},{"id":"T5","span":{"begin":1840,"end":1851},"obj":"Glycan"},{"id":"T6","span":{"begin":1873,"end":1880},"obj":"Glycan"},{"id":"T7","span":{"begin":1885,"end":1895},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G44653LT"},{"id":"A8","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G55560AP"},{"id":"A9","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G55560AP"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G15541SE"},{"id":"A10","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE"},{"id":"A4","pred":"glycosmos_id","subj":"T4","obj":"https://glycosmos.org/glycans/show/G47329OU"},{"id":"A11","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G47329OU"},{"id":"A5","pred":"glycosmos_id","subj":"T5","obj":"https://glycosmos.org/glycans/show/G47329OU"},{"id":"A12","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G47329OU"},{"id":"A6","pred":"glycosmos_id","subj":"T6","obj":"https://glycosmos.org/glycans/show/G44653LT"},{"id":"A13","pred":"image","subj":"T6","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT"},{"id":"A7","pred":"glycosmos_id","subj":"T7","obj":"https://glycosmos.org/glycans/show/G55560AP"},{"id":"A14","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G55560AP"}],"text":"Transglycosylation reactions of Bacillus stearothermophilus maltogenic amylase with acarbose and various acceptors.\nIt was observed that Bacillus stearothermophilus maltogenic amylase cleaved the first glycosidic bond of acarbose to produce glucose and a pseudotrisaccharide (PTS) that was transferred to C-6 of the glucose to give an alpha-(1--\u003e6) glycosidic linkage and the formation of isoacarbose. The addition of a number of different carbohydrates to the digest gave transfer products in which PTS was primarily attached alpha-(1--\u003e6) to D-glucose, D-mannose, D-galactose, and methyl alpha-D-glucopyranoside. With D-fructopyranose and D-xylopyranose, PTS was linked alpha-(1--\u003e5) and alpha-(1--\u003e4), respectively. PTS was primarily transferred to C-6 of the nonreducing residue of maltose, cellobiose, lactose, and gentiobiose. Lesser amounts of alpha-(1--\u003e3) and/or alpha-(1--\u003e4) transfer products were also observed for these carbohydrate acceptors. The major transfer product to sucrose gave PTS linked alpha-(1--\u003e4) to the glucose residue. alpha,alpha-Trehalose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4). Maltitol gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the glucopyranose residue. Raffinose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the D-galactopyranose residue. Maltotriose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the nonreducing end glucopyranose residue. Xylitol gave PTS linked alpha-(1--\u003e5) as the major product and D-glucitol gave PTS linked alpha-(1--\u003e6) as the only product. The structures of the transfer products were determined using thin-layer chromatography, high-performance ion chromatography, enzyme hydrolysis, methylation analysis and 13C NMR spectroscopy. The best acceptor was gentiobiose, followed closely by maltose and cellobiose, and the weakest acceptor was D-glucitol."}

{"project":"GlyCosmos15-NCBITAXON","denotations":[{"id":"T1","span":{"begin":32,"end":59},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":137,"end":164},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"1422"},{"id":"A2","pred":"db_id","subj":"T2","obj":"1422"}],"text":"Transglycosylation reactions of Bacillus stearothermophilus maltogenic amylase with acarbose and various acceptors.\nIt was observed that Bacillus stearothermophilus maltogenic amylase cleaved the first glycosidic bond of acarbose to produce glucose and a pseudotrisaccharide (PTS) that was transferred to C-6 of the glucose to give an alpha-(1--\u003e6) glycosidic linkage and the formation of isoacarbose. The addition of a number of different carbohydrates to the digest gave transfer products in which PTS was primarily attached alpha-(1--\u003e6) to D-glucose, D-mannose, D-galactose, and methyl alpha-D-glucopyranoside. With D-fructopyranose and D-xylopyranose, PTS was linked alpha-(1--\u003e5) and alpha-(1--\u003e4), respectively. PTS was primarily transferred to C-6 of the nonreducing residue of maltose, cellobiose, lactose, and gentiobiose. Lesser amounts of alpha-(1--\u003e3) and/or alpha-(1--\u003e4) transfer products were also observed for these carbohydrate acceptors. The major transfer product to sucrose gave PTS linked alpha-(1--\u003e4) to the glucose residue. alpha,alpha-Trehalose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4). Maltitol gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the glucopyranose residue. Raffinose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the D-galactopyranose residue. Maltotriose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the nonreducing end glucopyranose residue. Xylitol gave PTS linked alpha-(1--\u003e5) as the major product and D-glucitol gave PTS linked alpha-(1--\u003e6) as the only product. The structures of the transfer products were determined using thin-layer chromatography, high-performance ion chromatography, enzyme hydrolysis, methylation analysis and 13C NMR spectroscopy. The best acceptor was gentiobiose, followed closely by maltose and cellobiose, and the weakest acceptor was D-glucitol."}

{"project":"GlyCosmos15-UBERON","denotations":[{"id":"T1","span":{"begin":1693,"end":1698},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000119"},{"id":"A2","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0022303"}],"text":"Transglycosylation reactions of Bacillus stearothermophilus maltogenic amylase with acarbose and various acceptors.\nIt was observed that Bacillus stearothermophilus maltogenic amylase cleaved the first glycosidic bond of acarbose to produce glucose and a pseudotrisaccharide (PTS) that was transferred to C-6 of the glucose to give an alpha-(1--\u003e6) glycosidic linkage and the formation of isoacarbose. The addition of a number of different carbohydrates to the digest gave transfer products in which PTS was primarily attached alpha-(1--\u003e6) to D-glucose, D-mannose, D-galactose, and methyl alpha-D-glucopyranoside. With D-fructopyranose and D-xylopyranose, PTS was linked alpha-(1--\u003e5) and alpha-(1--\u003e4), respectively. PTS was primarily transferred to C-6 of the nonreducing residue of maltose, cellobiose, lactose, and gentiobiose. Lesser amounts of alpha-(1--\u003e3) and/or alpha-(1--\u003e4) transfer products were also observed for these carbohydrate acceptors. The major transfer product to sucrose gave PTS linked alpha-(1--\u003e4) to the glucose residue. alpha,alpha-Trehalose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4). Maltitol gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the glucopyranose residue. Raffinose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the D-galactopyranose residue. Maltotriose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the nonreducing end glucopyranose residue. Xylitol gave PTS linked alpha-(1--\u003e5) as the major product and D-glucitol gave PTS linked alpha-(1--\u003e6) as the only product. The structures of the transfer products were determined using thin-layer chromatography, high-performance ion chromatography, enzyme hydrolysis, methylation analysis and 13C NMR spectroscopy. The best acceptor was gentiobiose, followed closely by maltose and cellobiose, and the weakest acceptor was D-glucitol."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":115},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":116,"end":401},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":402,"end":614},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":615,"end":718},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":719,"end":832},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":833,"end":956},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":957,"end":1143},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1144,"end":1254},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1255,"end":1370},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":1371,"end":1500},"obj":"Sentence"},{"id":"TextSentencer_T11","span":{"begin":1501,"end":1625},"obj":"Sentence"},{"id":"TextSentencer_T12","span":{"begin":1626,"end":1817},"obj":"Sentence"},{"id":"TextSentencer_T13","span":{"begin":1818,"end":1937},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":115},"obj":"Sentence"},{"id":"T2","span":{"begin":116,"end":401},"obj":"Sentence"},{"id":"T3","span":{"begin":402,"end":614},"obj":"Sentence"},{"id":"T4","span":{"begin":615,"end":718},"obj":"Sentence"},{"id":"T5","span":{"begin":719,"end":832},"obj":"Sentence"},{"id":"T6","span":{"begin":833,"end":956},"obj":"Sentence"},{"id":"T7","span":{"begin":957,"end":1143},"obj":"Sentence"},{"id":"T8","span":{"begin":1144,"end":1254},"obj":"Sentence"},{"id":"T9","span":{"begin":1255,"end":1370},"obj":"Sentence"},{"id":"T10","span":{"begin":1371,"end":1500},"obj":"Sentence"},{"id":"T11","span":{"begin":1501,"end":1625},"obj":"Sentence"},{"id":"T12","span":{"begin":1626,"end":1817},"obj":"Sentence"},{"id":"T13","span":{"begin":1818,"end":1937},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Transglycosylation reactions of Bacillus stearothermophilus maltogenic amylase with acarbose and various acceptors.\nIt was observed that Bacillus stearothermophilus maltogenic amylase cleaved the first glycosidic bond of acarbose to produce glucose and a pseudotrisaccharide (PTS) that was transferred to C-6 of the glucose to give an alpha-(1--\u003e6) glycosidic linkage and the formation of isoacarbose. The addition of a number of different carbohydrates to the digest gave transfer products in which PTS was primarily attached alpha-(1--\u003e6) to D-glucose, D-mannose, D-galactose, and methyl alpha-D-glucopyranoside. With D-fructopyranose and D-xylopyranose, PTS was linked alpha-(1--\u003e5) and alpha-(1--\u003e4), respectively. PTS was primarily transferred to C-6 of the nonreducing residue of maltose, cellobiose, lactose, and gentiobiose. Lesser amounts of alpha-(1--\u003e3) and/or alpha-(1--\u003e4) transfer products were also observed for these carbohydrate acceptors. The major transfer product to sucrose gave PTS linked alpha-(1--\u003e4) to the glucose residue. alpha,alpha-Trehalose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4). Maltitol gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the glucopyranose residue. Raffinose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the D-galactopyranose residue. Maltotriose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the nonreducing end glucopyranose residue. Xylitol gave PTS linked alpha-(1--\u003e5) as the major product and D-glucitol gave PTS linked alpha-(1--\u003e6) as the only product. The structures of the transfer products were determined using thin-layer chromatography, high-performance ion chromatography, enzyme hydrolysis, methylation analysis and 13C NMR spectroscopy. The best acceptor was gentiobiose, followed closely by maltose and cellobiose, and the weakest acceptor was D-glucitol."}

{"project":"GlyCosmos15-Sentences","blocks":[{"id":"T1","span":{"begin":0,"end":115},"obj":"Sentence"},{"id":"T2","span":{"begin":116,"end":401},"obj":"Sentence"},{"id":"T3","span":{"begin":402,"end":614},"obj":"Sentence"},{"id":"T4","span":{"begin":615,"end":718},"obj":"Sentence"},{"id":"T5","span":{"begin":719,"end":832},"obj":"Sentence"},{"id":"T6","span":{"begin":833,"end":956},"obj":"Sentence"},{"id":"T7","span":{"begin":957,"end":1143},"obj":"Sentence"},{"id":"T8","span":{"begin":1144,"end":1254},"obj":"Sentence"},{"id":"T9","span":{"begin":1255,"end":1370},"obj":"Sentence"},{"id":"T10","span":{"begin":1371,"end":1500},"obj":"Sentence"},{"id":"T11","span":{"begin":1501,"end":1625},"obj":"Sentence"},{"id":"T12","span":{"begin":1626,"end":1817},"obj":"Sentence"},{"id":"T13","span":{"begin":1818,"end":1937},"obj":"Sentence"}],"text":"Transglycosylation reactions of Bacillus stearothermophilus maltogenic amylase with acarbose and various acceptors.\nIt was observed that Bacillus stearothermophilus maltogenic amylase cleaved the first glycosidic bond of acarbose to produce glucose and a pseudotrisaccharide (PTS) that was transferred to C-6 of the glucose to give an alpha-(1--\u003e6) glycosidic linkage and the formation of isoacarbose. The addition of a number of different carbohydrates to the digest gave transfer products in which PTS was primarily attached alpha-(1--\u003e6) to D-glucose, D-mannose, D-galactose, and methyl alpha-D-glucopyranoside. With D-fructopyranose and D-xylopyranose, PTS was linked alpha-(1--\u003e5) and alpha-(1--\u003e4), respectively. PTS was primarily transferred to C-6 of the nonreducing residue of maltose, cellobiose, lactose, and gentiobiose. Lesser amounts of alpha-(1--\u003e3) and/or alpha-(1--\u003e4) transfer products were also observed for these carbohydrate acceptors. The major transfer product to sucrose gave PTS linked alpha-(1--\u003e4) to the glucose residue. alpha,alpha-Trehalose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4). Maltitol gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the glucopyranose residue. Raffinose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the D-galactopyranose residue. Maltotriose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the nonreducing end glucopyranose residue. Xylitol gave PTS linked alpha-(1--\u003e5) as the major product and D-glucitol gave PTS linked alpha-(1--\u003e6) as the only product. The structures of the transfer products were determined using thin-layer chromatography, high-performance ion chromatography, enzyme hydrolysis, methylation analysis and 13C NMR spectroscopy. The best acceptor was gentiobiose, followed closely by maltose and cellobiose, and the weakest acceptor was D-glucitol."}

{"project":"GlyCosmos15-Glycan","denotations":[{"id":"T1","span":{"begin":786,"end":793},"obj":"Glycan"},{"id":"T2","span":{"begin":795,"end":805},"obj":"Glycan"},{"id":"T3","span":{"begin":807,"end":814},"obj":"Glycan"},{"id":"T4","span":{"begin":820,"end":831},"obj":"Glycan"},{"id":"T5","span":{"begin":1840,"end":1851},"obj":"Glycan"},{"id":"T6","span":{"begin":1873,"end":1880},"obj":"Glycan"},{"id":"T7","span":{"begin":1885,"end":1895},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G44653LT"},{"id":"A8","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G55560AP"},{"id":"A9","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G55560AP"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G15541SE"},{"id":"A10","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE"},{"id":"A4","pred":"glycosmos_id","subj":"T4","obj":"https://glycosmos.org/glycans/show/G47329OU"},{"id":"A11","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G47329OU"},{"id":"A5","pred":"glycosmos_id","subj":"T5","obj":"https://glycosmos.org/glycans/show/G47329OU"},{"id":"A12","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G47329OU"},{"id":"A6","pred":"glycosmos_id","subj":"T6","obj":"https://glycosmos.org/glycans/show/G44653LT"},{"id":"A13","pred":"image","subj":"T6","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT"},{"id":"A7","pred":"glycosmos_id","subj":"T7","obj":"https://glycosmos.org/glycans/show/G55560AP"},{"id":"A14","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G55560AP"}],"text":"Transglycosylation reactions of Bacillus stearothermophilus maltogenic amylase with acarbose and various acceptors.\nIt was observed that Bacillus stearothermophilus maltogenic amylase cleaved the first glycosidic bond of acarbose to produce glucose and a pseudotrisaccharide (PTS) that was transferred to C-6 of the glucose to give an alpha-(1--\u003e6) glycosidic linkage and the formation of isoacarbose. The addition of a number of different carbohydrates to the digest gave transfer products in which PTS was primarily attached alpha-(1--\u003e6) to D-glucose, D-mannose, D-galactose, and methyl alpha-D-glucopyranoside. With D-fructopyranose and D-xylopyranose, PTS was linked alpha-(1--\u003e5) and alpha-(1--\u003e4), respectively. PTS was primarily transferred to C-6 of the nonreducing residue of maltose, cellobiose, lactose, and gentiobiose. Lesser amounts of alpha-(1--\u003e3) and/or alpha-(1--\u003e4) transfer products were also observed for these carbohydrate acceptors. The major transfer product to sucrose gave PTS linked alpha-(1--\u003e4) to the glucose residue. alpha,alpha-Trehalose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4). Maltitol gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the glucopyranose residue. Raffinose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the D-galactopyranose residue. Maltotriose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the nonreducing end glucopyranose residue. Xylitol gave PTS linked alpha-(1--\u003e5) as the major product and D-glucitol gave PTS linked alpha-(1--\u003e6) as the only product. The structures of the transfer products were determined using thin-layer chromatography, high-performance ion chromatography, enzyme hydrolysis, methylation analysis and 13C NMR spectroscopy. The best acceptor was gentiobiose, followed closely by maltose and cellobiose, and the weakest acceptor was D-glucitol."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":32,"end":59},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":137,"end":164},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"1422"},{"id":"A2","pred":"db_id","subj":"T2","obj":"1422"}],"text":"Transglycosylation reactions of Bacillus stearothermophilus maltogenic amylase with acarbose and various acceptors.\nIt was observed that Bacillus stearothermophilus maltogenic amylase cleaved the first glycosidic bond of acarbose to produce glucose and a pseudotrisaccharide (PTS) that was transferred to C-6 of the glucose to give an alpha-(1--\u003e6) glycosidic linkage and the formation of isoacarbose. The addition of a number of different carbohydrates to the digest gave transfer products in which PTS was primarily attached alpha-(1--\u003e6) to D-glucose, D-mannose, D-galactose, and methyl alpha-D-glucopyranoside. With D-fructopyranose and D-xylopyranose, PTS was linked alpha-(1--\u003e5) and alpha-(1--\u003e4), respectively. PTS was primarily transferred to C-6 of the nonreducing residue of maltose, cellobiose, lactose, and gentiobiose. Lesser amounts of alpha-(1--\u003e3) and/or alpha-(1--\u003e4) transfer products were also observed for these carbohydrate acceptors. The major transfer product to sucrose gave PTS linked alpha-(1--\u003e4) to the glucose residue. alpha,alpha-Trehalose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4). Maltitol gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the glucopyranose residue. Raffinose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the D-galactopyranose residue. Maltotriose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the nonreducing end glucopyranose residue. Xylitol gave PTS linked alpha-(1--\u003e5) as the major product and D-glucitol gave PTS linked alpha-(1--\u003e6) as the only product. The structures of the transfer products were determined using thin-layer chromatography, high-performance ion chromatography, enzyme hydrolysis, methylation analysis and 13C NMR spectroscopy. The best acceptor was gentiobiose, followed closely by maltose and cellobiose, and the weakest acceptor was D-glucitol."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":1693,"end":1698},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000119"},{"id":"A2","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0022303"}],"text":"Transglycosylation reactions of Bacillus stearothermophilus maltogenic amylase with acarbose and various acceptors.\nIt was observed that Bacillus stearothermophilus maltogenic amylase cleaved the first glycosidic bond of acarbose to produce glucose and a pseudotrisaccharide (PTS) that was transferred to C-6 of the glucose to give an alpha-(1--\u003e6) glycosidic linkage and the formation of isoacarbose. The addition of a number of different carbohydrates to the digest gave transfer products in which PTS was primarily attached alpha-(1--\u003e6) to D-glucose, D-mannose, D-galactose, and methyl alpha-D-glucopyranoside. With D-fructopyranose and D-xylopyranose, PTS was linked alpha-(1--\u003e5) and alpha-(1--\u003e4), respectively. PTS was primarily transferred to C-6 of the nonreducing residue of maltose, cellobiose, lactose, and gentiobiose. Lesser amounts of alpha-(1--\u003e3) and/or alpha-(1--\u003e4) transfer products were also observed for these carbohydrate acceptors. The major transfer product to sucrose gave PTS linked alpha-(1--\u003e4) to the glucose residue. alpha,alpha-Trehalose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4). Maltitol gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the glucopyranose residue. Raffinose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the D-galactopyranose residue. Maltotriose gave two major products with PTS linked alpha-(1--\u003e6) and alpha-(1--\u003e4) to the nonreducing end glucopyranose residue. Xylitol gave PTS linked alpha-(1--\u003e5) as the major product and D-glucitol gave PTS linked alpha-(1--\u003e6) as the only product. The structures of the transfer products were determined using thin-layer chromatography, high-performance ion chromatography, enzyme hydrolysis, methylation analysis and 13C NMR spectroscopy. The best acceptor was gentiobiose, followed closely by maltose and cellobiose, and the weakest acceptor was D-glucitol."}