PubMed:10090338
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/10090338","sourcedb":"PubMed","sourceid":"10090338","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/10090338","text":"The relationship between Ca2+-ATPase and freely exchangeable Ca2+ in the dense tubules: a study in platelets from women.\nThe main aims of this work were to examine in women: the relationship between the freely exchangeable Ca2+ (FECa2+) in the dense tubules and the activity of the sarco(endo)plasmic reticulum (SER) Ca2+-ATPase (SERCA) in platelets, and the relationship of these parameters with blood pressure and serum lipoproteins. Platelets from 14 white and 13 black women in good health were studied. The FECa2+ was measured as the ionomycin-evoked Ca2+ release (in the presence of thapsigargin) in Ca2+-free medium. SERCA activity was measured as the thapsigargin sensitive, Ca2+ dependent and ouabain resistant, ATP hydrolyses in platelet membranes. Relative expressions of SERCA 2 and 3 isoforms and Ras-related protein (Rap) 1 in platelet membranes were determined by Western immunoblots. Highly significant correlations were observed for FECa2+ in the dense tubules with: 1) the maximal reaction velocity (Vmax) of the SERCA (r = 0.592, P = .0014), and 2) Rapl (r = 0.551, P = .0035). In addition, negative correlations were observed between FECa2+ in the dense tubules and age. No correlations were observed for these variables with blood pressure or serum lipoproteins. We conclude the FECa2+ and the Vmax of the SERCA are reliable indicators of Ca2+ load in platelets from women. However, in women, unlike previous observations in men, these platelet parameters are not correlated with blood pressure and serum lipoproteins.","tracks":[{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":41,"end":86},"obj":"NP"},{"id":"C3","span":{"begin":114,"end":119},"obj":"NP"},{"id":"C2","span":{"begin":99,"end":119},"obj":"NP"},{"id":"C4","span":{"begin":167,"end":172},"obj":"NP"},{"id":"C5","span":{"begin":199,"end":257},"obj":"NP"},{"id":"C6","span":{"begin":278,"end":336},"obj":"NP"},{"id":"C8","span":{"begin":397,"end":411},"obj":"NP"},{"id":"C9","span":{"begin":416,"end":434},"obj":"NP"},{"id":"C7","span":{"begin":397,"end":434},"obj":"NP"},{"id":"C10","span":{"begin":508,"end":518},"obj":"NP"},{"id":"C11","span":{"begin":739,"end":757},"obj":"NP"},{"id":"C12","span":{"begin":841,"end":859},"obj":"NP"},{"id":"C13","span":{"begin":950,"end":977},"obj":"NP"},{"id":"C15","span":{"begin":1027,"end":1036},"obj":"NP"},{"id":"C14","span":{"begin":987,"end":1036},"obj":"NP"},{"id":"C16","span":{"begin":1246,"end":1260},"obj":"NP"},{"id":"C17","span":{"begin":1264,"end":1282},"obj":"NP"},{"id":"C18","span":{"begin":1296,"end":1306},"obj":"NP"},{"id":"C19","span":{"begin":1311,"end":1332},"obj":"NP"},{"id":"C21","span":{"begin":1388,"end":1393},"obj":"NP"},{"id":"C20","span":{"begin":1373,"end":1393},"obj":"NP"},{"id":"C22","span":{"begin":1407,"end":1412},"obj":"NP"},{"id":"C24","span":{"begin":1501,"end":1515},"obj":"NP"},{"id":"C25","span":{"begin":1520,"end":1538},"obj":"NP"},{"id":"C23","span":{"begin":1501,"end":1538},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C4","obj":"C3"},{"id":"R2","pred":"coref-ident","subj":"C5","obj":"C1"},{"id":"R3","pred":"coref-ident","subj":"C12","obj":"C11"},{"id":"R4","pred":"coref-ident","subj":"C13","obj":"C5"},{"id":"R5","pred":"coref-ident","subj":"C15","obj":"C6"},{"id":"R6","pred":"coref-ident","subj":"C16","obj":"C8"},{"id":"R7","pred":"coref-ident","subj":"C17","obj":"C9"},{"id":"R8","pred":"coref-ident","subj":"C18","obj":"C10"},{"id":"R9","pred":"coref-ident","subj":"C19","obj":"C14"},{"id":"R10","pred":"coref-ident","subj":"C21","obj":"C4"},{"id":"R11","pred":"coref-ident","subj":"C20","obj":"C2"},{"id":"R12","pred":"coref-ident","subj":"C22","obj":"C21"},{"id":"R13","pred":"coref-ident","subj":"C24","obj":"C16"},{"id":"R14","pred":"coref-ident","subj":"C25","obj":"C17"},{"id":"R15","pred":"coref-ident","subj":"C23","obj":"C7"}],"attributes":[{"subj":"C1","pred":"source","obj":"genia-medco-coref"},{"subj":"C3","pred":"source","obj":"genia-medco-coref"},{"subj":"C2","pred":"source","obj":"genia-medco-coref"},{"subj":"C4","pred":"source","obj":"genia-medco-coref"},{"subj":"C5","pred":"source","obj":"genia-medco-coref"},{"subj":"C6","pred":"source","obj":"genia-medco-coref"},{"subj":"C8","pred":"source","obj":"genia-medco-coref"},{"subj":"C9","pred":"source","obj":"genia-medco-coref"},{"subj":"C7","pred":"source","obj":"genia-medco-coref"},{"subj":"C10","pred":"source","obj":"genia-medco-coref"},{"subj":"C11","pred":"source","obj":"genia-medco-coref"},{"subj":"C12","pred":"source","obj":"genia-medco-coref"},{"subj":"C13","pred":"source","obj":"genia-medco-coref"},{"subj":"C15","pred":"source","obj":"genia-medco-coref"},{"subj":"C14","pred":"source","obj":"genia-medco-coref"},{"subj":"C16","pred":"source","obj":"genia-medco-coref"},{"subj":"C17","pred":"source","obj":"genia-medco-coref"},{"subj":"C18","pred":"source","obj":"genia-medco-coref"},{"subj":"C19","pred":"source","obj":"genia-medco-coref"},{"subj":"C21","pred":"source","obj":"genia-medco-coref"},{"subj":"C20","pred":"source","obj":"genia-medco-coref"},{"subj":"C22","pred":"source","obj":"genia-medco-coref"},{"subj":"C24","pred":"source","obj":"genia-medco-coref"},{"subj":"C25","pred":"source","obj":"genia-medco-coref"},{"subj":"C23","pred":"source","obj":"genia-medco-coref"}]},{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":244,"end":249},"obj":"HP_0011088"},{"id":"T2","span":{"begin":964,"end":969},"obj":"HP_0011088"},{"id":"T3","span":{"begin":1168,"end":1173},"obj":"HP_0011088"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"},{"subj":"T2","pred":"source","obj":"PubmedHPO"},{"subj":"T3","pred":"source","obj":"PubmedHPO"}]},{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":25,"end":36},"obj":"protein"},{"id":"T2","span":{"begin":99,"end":108},"obj":"cell_type"},{"id":"T3","span":{"begin":282,"end":336},"obj":"protein"},{"id":"T4","span":{"begin":340,"end":349},"obj":"cell_type"},{"id":"T5","span":{"begin":416,"end":434},"obj":"protein"},{"id":"T6","span":{"begin":624,"end":629},"obj":"protein"},{"id":"T7","span":{"begin":783,"end":788},"obj":"protein"},{"id":"T8","span":{"begin":810,"end":837},"obj":"protein"},{"id":"T9","span":{"begin":1031,"end":1036},"obj":"protein"},{"id":"T10","span":{"begin":1068,"end":1072},"obj":"protein"},{"id":"T11","span":{"begin":1264,"end":1282},"obj":"protein"},{"id":"T12","span":{"begin":1327,"end":1332},"obj":"protein"},{"id":"T13","span":{"begin":1373,"end":1382},"obj":"cell_type"},{"id":"T14","span":{"begin":1520,"end":1538},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"jnlpba-st-training"},{"subj":"T2","pred":"source","obj":"jnlpba-st-training"},{"subj":"T3","pred":"source","obj":"jnlpba-st-training"},{"subj":"T4","pred":"source","obj":"jnlpba-st-training"},{"subj":"T5","pred":"source","obj":"jnlpba-st-training"},{"subj":"T6","pred":"source","obj":"jnlpba-st-training"},{"subj":"T7","pred":"source","obj":"jnlpba-st-training"},{"subj":"T8","pred":"source","obj":"jnlpba-st-training"},{"subj":"T9","pred":"source","obj":"jnlpba-st-training"},{"subj":"T10","pred":"source","obj":"jnlpba-st-training"},{"subj":"T11","pred":"source","obj":"jnlpba-st-training"},{"subj":"T12","pred":"source","obj":"jnlpba-st-training"},{"subj":"T13","pred":"source","obj":"jnlpba-st-training"},{"subj":"T14","pred":"source","obj":"jnlpba-st-training"}]},{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":120},"obj":"Sentence"},{"id":"S2","span":{"begin":121,"end":435},"obj":"Sentence"},{"id":"S3","span":{"begin":436,"end":507},"obj":"Sentence"},{"id":"S4","span":{"begin":508,"end":623},"obj":"Sentence"},{"id":"S5","span":{"begin":624,"end":758},"obj":"Sentence"},{"id":"S6","span":{"begin":759,"end":899},"obj":"Sentence"},{"id":"S7","span":{"begin":900,"end":1096},"obj":"Sentence"},{"id":"S8","span":{"begin":1097,"end":1190},"obj":"Sentence"},{"id":"S9","span":{"begin":1191,"end":1283},"obj":"Sentence"},{"id":"S10","span":{"begin":1284,"end":1394},"obj":"Sentence"},{"id":"S11","span":{"begin":1395,"end":1539},"obj":"Sentence"}],"attributes":[{"subj":"S1","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S2","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S3","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S4","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S5","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S6","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S7","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S8","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S9","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S10","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S11","pred":"source","obj":"pubmed-sentences-benchmark"}]},{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":25,"end":36},"obj":"protein_molecule"},{"id":"T2","span":{"begin":41,"end":65},"obj":"atom"},{"id":"T3","span":{"begin":99,"end":108},"obj":"cell_type"},{"id":"T4","span":{"begin":114,"end":119},"obj":"multi_cell"},{"id":"T5","span":{"begin":167,"end":172},"obj":"multi_cell"},{"id":"T6","span":{"begin":203,"end":227},"obj":"atom"},{"id":"T7","span":{"begin":229,"end":235},"obj":"atom"},{"id":"T8","span":{"begin":282,"end":310},"obj":"cell_component"},{"id":"T9","span":{"begin":312,"end":315},"obj":"cell_component"},{"id":"T10","span":{"begin":317,"end":328},"obj":"protein_family_or_group"},{"id":"T11","span":{"begin":330,"end":335},"obj":"protein_molecule"},{"id":"T12","span":{"begin":340,"end":349},"obj":"cell_type"},{"id":"T13","span":{"begin":397,"end":411},"obj":"other_name"},{"id":"T14","span":{"begin":416,"end":434},"obj":"protein_family_or_group"},{"id":"T15","span":{"begin":454,"end":459},"obj":"multi_cell"},{"id":"T16","span":{"begin":467,"end":478},"obj":"multi_cell"},{"id":"T17","span":{"begin":512,"end":518},"obj":"atom"},{"id":"T18","span":{"begin":539,"end":555},"obj":"other_name"},{"id":"T19","span":{"begin":556,"end":560},"obj":"atom"},{"id":"T20","span":{"begin":606,"end":610},"obj":"atom"},{"id":"T21","span":{"begin":624,"end":629},"obj":"protein_molecule"},{"id":"T22","span":{"begin":683,"end":687},"obj":"atom"},{"id":"T23","span":{"begin":702,"end":709},"obj":"other_organic_compound"},{"id":"T24","span":{"begin":739,"end":757},"obj":"cell_component"},{"id":"T25","span":{"begin":783,"end":788},"obj":"protein_molecule"},{"id":"T26","span":{"begin":810,"end":837},"obj":"protein_molecule"},{"id":"T27","span":{"begin":841,"end":859},"obj":"cell_component"},{"id":"T28","span":{"begin":879,"end":898},"obj":"other_name"},{"id":"T29","span":{"begin":950,"end":956},"obj":"atom"},{"id":"T30","span":{"begin":1031,"end":1036},"obj":"protein_molecule"},{"id":"T31","span":{"begin":1068,"end":1072},"obj":"protein_molecule"},{"id":"T32","span":{"begin":1154,"end":1160},"obj":"atom"},{"id":"T33","span":{"begin":1246,"end":1260},"obj":"other_name"},{"id":"T34","span":{"begin":1264,"end":1282},"obj":"protein_family_or_group"},{"id":"T35","span":{"begin":1300,"end":1306},"obj":"atom"},{"id":"T36","span":{"begin":1327,"end":1332},"obj":"protein_molecule"},{"id":"T37","span":{"begin":1360,"end":1364},"obj":"atom"},{"id":"T38","span":{"begin":1373,"end":1382},"obj":"cell_type"},{"id":"T39","span":{"begin":1388,"end":1393},"obj":"multi_cell"},{"id":"T40","span":{"begin":1407,"end":1412},"obj":"multi_cell"},{"id":"T41","span":{"begin":1446,"end":1449},"obj":"multi_cell"},{"id":"T42","span":{"begin":1501,"end":1515},"obj":"other_name"},{"id":"T43","span":{"begin":1520,"end":1538},"obj":"protein_family_or_group"}],"attributes":[{"subj":"T1","pred":"source","obj":"GENIAcorpus"},{"subj":"T2","pred":"source","obj":"GENIAcorpus"},{"subj":"T3","pred":"source","obj":"GENIAcorpus"},{"subj":"T4","pred":"source","obj":"GENIAcorpus"},{"subj":"T5","pred":"source","obj":"GENIAcorpus"},{"subj":"T6","pred":"source","obj":"GENIAcorpus"},{"subj":"T7","pred":"source","obj":"GENIAcorpus"},{"subj":"T8","pred":"source","obj":"GENIAcorpus"},{"subj":"T9","pred":"source","obj":"GENIAcorpus"},{"subj":"T10","pred":"source","obj":"GENIAcorpus"},{"subj":"T11","pred":"source","obj":"GENIAcorpus"},{"subj":"T12","pred":"source","obj":"GENIAcorpus"},{"subj":"T13","pred":"source","obj":"GENIAcorpus"},{"subj":"T14","pred":"source","obj":"GENIAcorpus"},{"subj":"T15","pred":"source","obj":"GENIAcorpus"},{"subj":"T16","pred":"source","obj":"GENIAcorpus"},{"subj":"T17","pred":"source","obj":"GENIAcorpus"},{"subj":"T18","pred":"source","obj":"GENIAcorpus"},{"subj":"T19","pred":"source","obj":"GENIAcorpus"},{"subj":"T20","pred":"source","obj":"GENIAcorpus"},{"subj":"T21","pred":"source","obj":"GENIAcorpus"},{"subj":"T22","pred":"source","obj":"GENIAcorpus"},{"subj":"T23","pred":"source","obj":"GENIAcorpus"},{"subj":"T24","pred":"source","obj":"GENIAcorpus"},{"subj":"T25","pred":"source","obj":"GENIAcorpus"},{"subj":"T26","pred":"source","obj":"GENIAcorpus"},{"subj":"T27","pred":"source","obj":"GENIAcorpus"},{"subj":"T28","pred":"source","obj":"GENIAcorpus"},{"subj":"T29","pred":"source","obj":"GENIAcorpus"},{"subj":"T30","pred":"source","obj":"GENIAcorpus"},{"subj":"T31","pred":"source","obj":"GENIAcorpus"},{"subj":"T32","pred":"source","obj":"GENIAcorpus"},{"subj":"T33","pred":"source","obj":"GENIAcorpus"},{"subj":"T34","pred":"source","obj":"GENIAcorpus"},{"subj":"T35","pred":"source","obj":"GENIAcorpus"},{"subj":"T36","pred":"source","obj":"GENIAcorpus"},{"subj":"T37","pred":"source","obj":"GENIAcorpus"},{"subj":"T38","pred":"source","obj":"GENIAcorpus"},{"subj":"T39","pred":"source","obj":"GENIAcorpus"},{"subj":"T40","pred":"source","obj":"GENIAcorpus"},{"subj":"T41","pred":"source","obj":"GENIAcorpus"},{"subj":"T42","pred":"source","obj":"GENIAcorpus"},{"subj":"T43","pred":"source","obj":"GENIAcorpus"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"genia-medco-coref","color":"#d8ec93","default":true},{"id":"PubmedHPO","color":"#e693ec"},{"id":"jnlpba-st-training","color":"#93eccc"},{"id":"pubmed-sentences-benchmark","color":"#ecb293"},{"id":"GENIAcorpus","color":"#9893ec"}]}]}}